Restriction map for
pF1KB4718
(Circular) (Six-base) MAPSORT of: pF1KB4718.seq Check: 8390 from: 1 to: 3746
pF1KB4718 3746 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 290 784 290
Size: 494 3252
Acc65I G'GTAC_C
Cuts at: 689 762 689
Size: 73 3673
AclI AA'CG_TT
Cuts at: 3586 3586
Size: 3746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 2003 2356 3018 1571
Size: 432 353 662 2299
Fragments arranged by size:
2299 662 432 353
AflII C'TTAA_G
Cuts at: 385 385
Size: 3746
AflIII A'CryG_T
Cuts at: 1098 2898 3179 1098
Size: 1800 281 1665
Fragments arranged by size:
1800 1665 281
AlwNI CAG_nnn'CTG
Cuts at: 414 1051 2489 414
Size: 637 1438 1671
Fragments arranged by size:
1671 1438 637
ApaI G_GGCC'C
Cuts at: 275 705 275
Size: 430 3316
ApaLI G'TGCA_C
Cuts at: 177 2584 177
Size: 2407 1339
ApoI r'AATT_y
Cuts at: 750 3099 3355 750
Size: 2349 256 1141
Fragments arranged by size:
2349 1141 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
AvaI C'yCGr_G
Cuts at: 121 766 121
Size: 645 3101
BamHI G'GATC_C
Cuts at: 771 771
Size: 3746
BanI G'GyrC_C
Cuts at: 166 689 762 1176 1424 1459 166
Size: 523 73 414 248 35 2453
Fragments arranged by size:
2453 523 414 248 73 35
BanII G_rGCy'C
Cuts at: 128 275 705 760 1790 128
Size: 147 430 55 1030 2084
Fragments arranged by size:
2084 1030 430 147 55
BbeI G_GCGC'C
Cuts at: 170 1180 1428 170
Size: 1010 248 2488
Fragments arranged by size:
2488 1010 248
BciVI GTATCCnnnnn_n'
Cuts at: 1644 2700 3746 1644
Size: 1056 1046 1644
Fragments arranged by size:
1644 1056 1046
BclI T'GATC_A
Cuts at: 1266 1266
Size: 3746
BglII A'GATC_T
Cuts at: 1261 3298 1261
Size: 2037 1709
BlpI GC'TnA_GC
Cuts at: 858 858
Size: 3746
Bme1580I G_kGCm'C
Cuts at: 181 275 705 1371 1464 2588 181
Size: 94 430 666 93 1124 1339
Fragments arranged by size:
1339 1124 666 430 94 93
BmrI ACTGGGnnnn_n'
Cuts at: 171 958 1039 1374 3518 171
Size: 787 81 335 2144 399
Fragments arranged by size:
2144 787 399 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 3746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 412 168
Size: 244 3502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1801 2515 2813 922
Size: 879 714 298 1855
Fragments arranged by size:
1855 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3450 3450
Size: 3746
BsaAI yAC'GTr
Cuts at: 1099 1099
Size: 3746
BsaBI GATnn'nnATC
Cuts at: 6 1260 3297 6
Size: 1254 2037 455
Fragments arranged by size:
2037 1254 455
BsaHI Gr'CG_yC
Cuts at: 167 206 1177 1425 2127 3623 167
Size: 39 971 248 702 1496 290
Fragments arranged by size:
1496 971 702 290 248 39
BsaWI w'CCGG_w
Cuts at: 935 1456 2134 2545 2692 2966 935
Size: 521 678 411 147 274 1715
Fragments arranged by size:
1715 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 598 628 598
Size: 30 3716
BseYI C'CCAG_C
Cuts at: 190 497 1171 1929 2594 3002 190
Size: 307 674 758 665 408 934
Fragments arranged by size:
934 758 674 665 408 307
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 364
Size: 3746
BsiEI CG_ry'CG
Cuts at: 104 1288 1334 2564 104
Size: 1184 46 1230 1286
Fragments arranged by size:
1286 1230 1184 46
BsiHKAI G_wGCw'C
Cuts at: 137 181 760 1538 1728 2588 137
Size: 44 579 778 190 860 1295
Fragments arranged by size:
1295 860 778 579 190 44
Bsp1286I G_dGCh'C
Cuts at: 128 137 181 275 705 760 1371 1464 1538
Size: 9 44 94 430 55 611 93 74
Cuts at: 1538 1728 1790 2588 128
Size: 190 62 798 1286
Fragments arranged by size:
1286 798 611 430 190 94 93 74
62 55 44 9
BspHI T'CATG_A
Cuts at: 2178 2178
Size: 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 422 572 796 1010 1312 1693 422
Size: 150 224 214 302 381 2475
Fragments arranged by size:
2475 381 302 224 214 150
BsrBI CCG'CTC
Cuts at: 50 2038 2092 3576 3741 50
Size: 1988 54 1484 165 55
Fragments arranged by size:
1988 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1658 1658
Size: 3746
BsrFI r'CCGG_y
Cuts at: 1744 1925 1744
Size: 181 3565
BsrGI T'GTAC_A
Cuts at: 446 446
Size: 3746
BssSI C'ACGA_G
Cuts at: 2017 2725 2017
Size: 708 3038
BstAPI GCAn_nnn'nTGC
Cuts at: 1123 1123
Size: 3746
BstBI TT'CG_AA
Cuts at: 2107 2107
Size: 3746
BstEII G'GTnAC_C
Cuts at: 625 625
Size: 3746
BstXI CCAn_nnnn'nTGG
Cuts at: 246 246
Size: 3746
BstYI r'GATC_y
Cuts at: 1 771 1238 1261 1596 1842 2148 2160 2246
Size: 770 467 23 335 246 306 12 86
Cuts at: 2246 3298 1
Size: 1052 449
Fragments arranged by size:
1052 770 467 449 335 306 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1696 1877 2040 1696
Size: 181 163 3402
Fragments arranged by size:
3402 181 163
BtsI GCAGTG_nn'
Cuts at: 989 989
Size: 3746
DraI TTT'AAA
Cuts at: 745 745
Size: 3746
DrdI GACnn_nn'nnGTC
Cuts at: 1452 2796 1452
Size: 1344 2402
EaeI y'GGCC_r
Cuts at: 1331 1505 1896 1923 3268 1331
Size: 174 391 27 1345 1809
Fragments arranged by size:
1809 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1331 1331
Size: 3746
EarI CTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1954 2696 2842 3556 1954
Size: 742 146 714 2144
Fragments arranged by size:
2144 742 714 146
EcoICRI GAG'CTC
Cuts at: 758 758
Size: 3746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 412 1571 2003 2356 3018 168
Size: 244 1159 432 353 662 896
Fragments arranged by size:
1159 896 662 432 353 244
EcoO109I rG'GnC_Cy
Cuts at: 271 272 702 885 271
Size: 1 430 183 3132
Fragments arranged by size:
3132 430 183 1
EcoRI G'AATT_C
Cuts at: 750 750
Size: 3746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1765 1765
Size: 3746
FspI TGC'GCA
Cuts at: 1527 1527
Size: 3746
HaeII r_GCGC'y
Cuts at: 170 1180 1428 2658 3429 170
Size: 1010 248 1230 771 487
Fragments arranged by size:
1230 1010 771 487 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 598 630 1588 1618 1620 1650 598
Size: 32 958 30 2 30 2694
Fragments arranged by size:
2694 958 32 30 30 2
HincII GTy'rAC
Cuts at: 399 785 399
Size: 386 3360
HindIII A'AGCT_T
Cuts at: 362 362
Size: 3746
KasI G'GCGC_C
Cuts at: 166 1176 1424 166
Size: 1010 248 2488
Fragments arranged by size:
2488 1010 248
KpnI G_GTAC'C
Cuts at: 693 766 693
Size: 73 3673
MluI A'CGCG_T
Cuts at: 3179 3179
Size: 3746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 245 2505 2689 245
Size: 2260 184 1302
Fragments arranged by size:
2260 1302 184
MscI TGG'CCA
Cuts at: 1507 1507
Size: 3746
MslI CAynn'nnrTG
Cuts at: 331 1735 1862 331
Size: 1404 127 2215
Fragments arranged by size:
2215 1404 127
MspA1I CmG'CkG
Cuts at: 146 686 857 1171 1531 2315 2560 146
Size: 540 171 314 360 784 245 1332
Fragments arranged by size:
1332 784 540 360 314 245 171
NaeI GCC'GGC
Cuts at: 1927 1927
Size: 3746
NarI GG'CG_CC
Cuts at: 167 1177 1425 167
Size: 1010 248 2488
Fragments arranged by size:
2488 1010 248
NgoMIV G'CCGG_C
Cuts at: 1925 1925
Size: 3746
NspI r_CATG'y
Cuts at: 185 612 799 1299 2902 185
Size: 427 187 500 1603 1029
Fragments arranged by size:
1603 1029 500 427 187
PciI A'CATG_T
Cuts at: 2898 2898
Size: 3746
PmeI GTTT'AAAC
Cuts at: 745 745
Size: 3746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2192 2224 2192
Size: 32 3714
PspOMI G'GGCC_C
Cuts at: 271 701 271
Size: 430 3316
PstI C_TGCA'G
Cuts at: 685 793 1478 685
Size: 108 685 2953
Fragments arranged by size:
2953 685 108
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3746
PvuII CAG'CTG
Cuts at: 146 686 1171 1531 146
Size: 540 485 360 2361
Fragments arranged by size:
2361 540 485 360
RsrII CG'GwC_CG
Cuts at: 1941 1941
Size: 3746
SacI G_AGCT'C
Cuts at: 760 760
Size: 3746
SalI G'TCGA_C
Cuts at: 783 783
Size: 3746
SapI GCTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
SbfI CC_TGCA'GG
Cuts at: 793 793
Size: 3746
ScaI AGT'ACT
Cuts at: 3263 3263
Size: 3746
SfcI C'TryA_G
Cuts at: 33 681 789 1474 2442 2633 33
Size: 648 108 685 968 191 1146
Fragments arranged by size:
1146 968 685 648 191 108
SfoI GGC'GCC
Cuts at: 168 1178 1426 168
Size: 1010 248 2488
Fragments arranged by size:
2488 1010 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
SmaI CCC'GGG
Cuts at: 768 768
Size: 3746
SmlI C'TyrA_G
Cuts at: 385 901 1816 2530 2792 385
Size: 516 915 714 262 1339
Fragments arranged by size:
1339 915 714 516 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3746
SphI G_CATG'C
Cuts at: 799 1299 799
Size: 500 3246
StyI C'CwwG_G
Cuts at: 115 239 880 1234 115
Size: 124 641 354 2627
Fragments arranged by size:
2627 641 354 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 619 1329 1462 1921 2130 619
Size: 710 133 459 209 2235
Fragments arranged by size:
2235 710 459 209 133
TatI w'GTAC_w
Cuts at: 347 446 3261 347
Size: 99 2815 832
Fragments arranged by size:
2815 832 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 3714
Tth111I GACn'n_nGTC
Cuts at: 1543 1543
Size: 3746
XbaI T'CTAG_A
Cuts at: 777 777
Size: 3746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1241 1241
Size: 3746
XmaI C'CCGG_G
Cuts at: 766 766
Size: 3746
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BseRI BsiWI BsmI BsmBI BspEI
BssHII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI