Restriction map for
pF1KB4707
(Circular) (Six-base) MAPSORT of: pF1KB4707.seq Check: 2286 from: 1 to: 4199
pF1KB4707 4199 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 287 287
Size: 4199
AccI GT'mk_AC
Cuts at: 1237 1237
Size: 4199
Acc65I G'GTAC_C
Cuts at: 1215 1215
Size: 4199
AclI AA'CG_TT
Cuts at: 4039 4039
Size: 4199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 698 1051 2024 2456 3203 3471 679
Size: 19 353 973 432 747 268 1407
Fragments arranged by size:
1407 973 747 432 353 268 19
AflIII A'CryG_T
Cuts at: 522 1551 2655 3632 522
Size: 1029 1104 977 1089
Fragments arranged by size:
1104 1089 1029 977
AgeI A'CCGG_T
Cuts at: 2587 2587
Size: 4199
AleI CACnn'nnGTG
Cuts at: 136 615 136
Size: 479 3720
AlwNI CAG_nnn'CTG
Cuts at: 384 1504 3071 384
Size: 1120 1567 1512
Fragments arranged by size:
1567 1512 1120
ApaLI G'TGCA_C
Cuts at: 2969 2969
Size: 4199
ApoI r'AATT_y
Cuts at: 1203 3552 3808 1203
Size: 2349 256 1594
Fragments arranged by size:
2349 1594 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4199
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
AvaI C'yCGr_G
Cuts at: 537 1219 537
Size: 682 3517
BamHI G'GATC_C
Cuts at: 1224 1224
Size: 4199
BanI G'GyrC_C
Cuts at: 1215 1629 1877 1912 1215
Size: 414 248 35 3502
Fragments arranged by size:
3502 414 248 35
BanII G_rGCy'C
Cuts at: 470 758 1213 2243 470
Size: 288 455 1030 2426
Fragments arranged by size:
2426 1030 455 288
BbeI G_GCGC'C
Cuts at: 1633 1881 1633
Size: 248 3951
BbsI GAAGACnn'nnnn_
Cuts at: 319 319
Size: 4199
BbvCI CC'TCA_GC
Cuts at: 738 738
Size: 4199
BciVI GTATCCnnnnn_n'
Cuts at: 733 2097 2858 4199 733
Size: 1364 761 1341 733
Fragments arranged by size:
1364 1341 761 733
BclI T'GATC_A
Cuts at: 1719 1719
Size: 4199
BfrBI ATG'CAT
Cuts at: 232 232
Size: 4199
BglII A'GATC_T
Cuts at: 1714 3751 1714
Size: 2037 2162
BlpI GC'TnA_GC
Cuts at: 1311 1311
Size: 4199
Bme1580I G_kGCm'C
Cuts at: 1824 1917 2973 1824
Size: 93 1056 3050
Fragments arranged by size:
3050 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1411 1492 1827 3971 1411
Size: 81 335 2144 1639
Fragments arranged by size:
2144 1639 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 4199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 772
Size: 4199
Bpu10I CC'TnA_GC
Cuts at: 738 738
Size: 4199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 929 1085 1375 2254 2746 3044 228
Size: 701 156 290 879 492 298 1383
Fragments arranged by size:
1383 879 701 492 298 290 156
BsaI GGTCTCn'nnnn_
Cuts at: 921 3903 921
Size: 2982 1217
BsaAI yAC'GTr
Cuts at: 523 1552 523
Size: 1029 3170
BsaBI GATnn'nnATC
Cuts at: 6 1713 3750 6
Size: 1707 2037 455
Fragments arranged by size:
2037 1707 455
BsaHI Gr'CG_yC
Cuts at: 284 1630 1878 2580 4076 284
Size: 1346 248 702 1496 407
Fragments arranged by size:
1496 1346 702 407 248
BsaWI w'CCGG_w
Cuts at: 1388 1909 2587 2861 3008 3419 1388
Size: 521 678 274 147 411 2168
Fragments arranged by size:
2168 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 880 1013 880
Size: 133 4066
BseYI C'CCAG_C
Cuts at: 1624 2382 2959 3455 1624
Size: 758 577 496 2368
Fragments arranged by size:
2368 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1741 1787 2995 104
Size: 1637 46 1208 1308
Fragments arranged by size:
1637 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 758 1213 1991 2181 2973 758
Size: 455 778 190 792 1984
Fragments arranged by size:
1984 792 778 455 190
BsmI GAATG_Cn'
Cuts at: 945 945
Size: 4199
Bsp1286I G_dGCh'C
Cuts at: 470 758 1213 1824 1917 1991 2181 2243 2973
Size: 288 455 611 93 74 190 62 730
Cuts at: 2973 470
Size: 1696
Fragments arranged by size:
1696 730 611 455 288 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3419 3419
Size: 4199
BspHI T'CATG_A
Cuts at: 3375 3375
Size: 4199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 939 1249 1463 1765 2146 939
Size: 310 214 302 381 2992
Fragments arranged by size:
2992 381 310 302 214
BsrBI CCG'CTC
Cuts at: 50 2491 2545 4029 4194 50
Size: 2441 54 1484 165 55
Fragments arranged by size:
2441 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2111 2111
Size: 4199
BsrFI r'CCGG_y
Cuts at: 2197 2378 2587 2197
Size: 181 209 3809
Fragments arranged by size:
3809 209 181
BssSI C'ACGA_G
Cuts at: 2470 2828 2470
Size: 358 3841
BstAPI GCAn_nnn'nTGC
Cuts at: 1576 1576
Size: 4199
BstBI TT'CG_AA
Cuts at: 2560 2560
Size: 4199
BstXI CCAn_nnnn'nTGG
Cuts at: 750 750
Size: 4199
BstYI r'GATC_y
Cuts at: 1 1138 1224 1691 1714 2049 2295 3307 3393
Size: 1137 86 467 23 335 246 1012 86
Cuts at: 3393 3405 3751 1
Size: 12 346 449
Fragments arranged by size:
1137 1012 467 449 346 335 246 86
86 23 12
BtgI C'CryG_G
Cuts at: 462 782 462
Size: 320 3879
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2149 2330 2493 2149
Size: 181 163 3855
Fragments arranged by size:
3855 181 163
BtsI GCAGTG_nn'
Cuts at: 1442 1442
Size: 4199
DraI TTT'AAA
Cuts at: 1198 1198
Size: 4199
DrdI GACnn_nn'nnGTC
Cuts at: 550 1905 2763 550
Size: 1355 858 1986
Fragments arranged by size:
1986 1355 858
EaeI y'GGCC_r
Cuts at: 1784 1958 2349 2376 3721 1784
Size: 174 391 27 1345 2262
Fragments arranged by size:
2262 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1784 1784
Size: 4199
EarI CTCTTCn'nnn_
Cuts at: 833 2222 2432 833
Size: 1389 210 2600
Fragments arranged by size:
2600 1389 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2407 2717 2863 4009 2407
Size: 310 146 1146 2597
Fragments arranged by size:
2597 1146 310 146
EcoICRI GAG'CTC
Cuts at: 756 1211 756
Size: 455 3744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 698 772 1051 2024 2456 3203 3471 679
Size: 19 74 279 973 432 747 268 1407
Fragments arranged by size:
1407 973 747 432 279 268 74 19
EcoO109I rG'GnC_Cy
Cuts at: 1338 1338
Size: 4199
EcoRI G'AATT_C
Cuts at: 1203 1203
Size: 4199
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 4199
FspI TGC'GCA
Cuts at: 1980 1980
Size: 4199
HaeII r_GCGC'y
Cuts at: 1633 1881 2903 3882 1633
Size: 248 1022 979 1950
Fragments arranged by size:
1950 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 618 650 1000 1032 1081 1113 1123 1155 2041
Size: 32 350 32 49 32 10 32 886
Cuts at: 2041 2071 2073 2103 618
Size: 30 2 30 2714
Fragments arranged by size:
2714 886 350 49 32 32 32 32
30 30 10 2
HincII GTy'rAC
Cuts at: 1238 1238
Size: 4199
HindIII A'AGCT_T
Cuts at: 246 246
Size: 4199
KasI G'GCGC_C
Cuts at: 1629 1877 1629
Size: 248 3951
KpnI G_GTAC'C
Cuts at: 1219 1219
Size: 4199
MfeI C'AATT_G
Cuts at: 317 317
Size: 4199
MluI A'CGCG_T
Cuts at: 3632 3632
Size: 4199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2870 3054 2870
Size: 184 4015
MscI TGG'CCA
Cuts at: 1960 1960
Size: 4199
MslI CAynn'nnrTG
Cuts at: 136 275 615 2188 2315 136
Size: 139 340 1573 127 2020
Fragments arranged by size:
2020 1573 340 139 127
MspA1I CmG'CkG
Cuts at: 160 1191 1310 1624 1984 2997 3242 160
Size: 1031 119 314 360 1013 245 1117
Fragments arranged by size:
1117 1031 1013 360 314 245 119
NaeI GCC'GGC
Cuts at: 2380 2380
Size: 4199
NarI GG'CG_CC
Cuts at: 1630 1878 1630
Size: 248 3951
NcoI C'CATG_G
Cuts at: 782 782
Size: 4199
NdeI CA'TA_TG
Cuts at: 1176 1176
Size: 4199
NgoMIV G'CCGG_C
Cuts at: 2378 2378
Size: 4199
NsiI A_TGCA'T
Cuts at: 234 234
Size: 4199
NspI r_CATG'y
Cuts at: 1252 1752 2659 1252
Size: 500 907 2792
Fragments arranged by size:
2792 907 500
PciI A'CATG_T
Cuts at: 2655 2655
Size: 4199
PmeI GTTT'AAAC
Cuts at: 1198 1198
Size: 4199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3338 3370 3338
Size: 32 4167
PstI C_TGCA'G
Cuts at: 1146 1246 1931 1146
Size: 100 685 3414
Fragments arranged by size:
3414 685 100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4199
PvuII CAG'CTG
Cuts at: 160 1191 1624 1984 160
Size: 1031 433 360 2375
Fragments arranged by size:
2375 1031 433 360
RsrII CG'GwC_CG
Cuts at: 2394 2394
Size: 4199
SacI G_AGCT'C
Cuts at: 758 1213 758
Size: 455 3744
SalI G'TCGA_C
Cuts at: 1236 1236
Size: 4199
SapI GCTCTTCn'nnn_
Cuts at: 2222 2432 2222
Size: 210 3989
SbfI CC_TGCA'GG
Cuts at: 1246 1246
Size: 4199
ScaI AGT'ACT
Cuts at: 3716 3716
Size: 4199
SfcI C'TryA_G
Cuts at: 33 1142 1242 1927 2920 3111 33
Size: 1109 100 685 993 191 1121
Fragments arranged by size:
1121 1109 993 685 191 100
SfoI GGC'GCC
Cuts at: 1631 1879 1631
Size: 248 3951
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
SmaI CCC'GGG
Cuts at: 1221 1221
Size: 4199
SmlI C'TyrA_G
Cuts at: 243 944 1100 1354 2269 2761 3023 243
Size: 701 156 254 915 492 262 1419
Fragments arranged by size:
1419 915 701 492 262 254 156
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4199
SphI G_CATG'C
Cuts at: 1252 1752 1252
Size: 500 3699
StuI AGG'CCT
Cuts at: 703 703
Size: 4199
StyI C'CwwG_G
Cuts at: 782 1333 1687 782
Size: 551 354 3294
Fragments arranged by size:
3294 551 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 898 1782 1915 2374 2583 898
Size: 884 133 459 209 2514
Fragments arranged by size:
2514 884 459 209 133
TatI w'GTAC_w
Cuts at: 3714 3714
Size: 4199
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3716 3748 3716
Size: 32 4167
Tth111I GACn'n_nGTC
Cuts at: 1996 1996
Size: 4199
XbaI T'CTAG_A
Cuts at: 1230 1230
Size: 4199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1694 1694
Size: 4199
XmaI C'CCGG_G
Cuts at: 1219 1219
Size: 4199
ZraI GAC'GTC
Cuts at: 285 285
Size: 4199
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI