Restriction map for
pF1KB4675
(Circular) (Six-base) MAPSORT of: pF1KB4675.seq Check: 7223 from: 1 to: 4595
pF1KB4675 4595 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 508 508
Size: 4595
AccI GT'mk_AC
Cuts at: 1633 1633
Size: 4595
Acc65I G'GTAC_C
Cuts at: 1611 1611
Size: 4595
AclI AA'CG_TT
Cuts at: 1018 4435 1018
Size: 3417 1178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 533 908 910 973 1388 2420 2852 3599
Size: 15 375 2 63 415 1032 432 747
Cuts at: 3599 3867 518
Size: 268 1246
Fragments arranged by size:
1246 1032 747 432 415 375 268 63
15 2
AflII C'TTAA_G
Cuts at: 1138 1138
Size: 4595
AflIII A'CryG_T
Cuts at: 1947 3051 4028 1947
Size: 1104 977 2514
Fragments arranged by size:
2514 1104 977
AgeI A'CCGG_T
Cuts at: 2983 2983
Size: 4595
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1046 1046
Size: 4595
AlwNI CAG_nnn'CTG
Cuts at: 886 1900 3467 886
Size: 1014 1567 2014
Fragments arranged by size:
2014 1567 1014
ApaLI G'TGCA_C
Cuts at: 3365 3365
Size: 4595
ApoI r'AATT_y
Cuts at: 148 1150 1225 1599 3948 4204 148
Size: 1002 75 374 2349 256 539
Fragments arranged by size:
2349 1002 539 374 256 75
AseI AT'TA_AT
Cuts at: 20 20
Size: 4595
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4595
AvaI C'yCGr_G
Cuts at: 1615 1615
Size: 4595
BamHI G'GATC_C
Cuts at: 1620 1620
Size: 4595
BanI G'GyrC_C
Cuts at: 520 1611 2025 2273 2308 520
Size: 1091 414 248 35 2807
Fragments arranged by size:
2807 1091 414 248 35
BanII G_rGCy'C
Cuts at: 1609 2639 1609
Size: 1030 3565
BbeI G_GCGC'C
Cuts at: 2029 2277 2029
Size: 248 4347
BbsI GAAGACnn'nnnn_
Cuts at: 360 383 360
Size: 23 4572
BbvCI CC'TCA_GC
Cuts at: 354 354
Size: 4595
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4561
BciVI GTATCCnnnnn_n'
Cuts at: 713 1348 2493 3254 4595 713
Size: 635 1145 761 1341 713
Fragments arranged by size:
1341 1145 761 713 635
BclI T'GATC_A
Cuts at: 2115 2115
Size: 4595
BglII A'GATC_T
Cuts at: 2110 4147 2110
Size: 2037 2558
BlpI GC'TnA_GC
Cuts at: 200 931 1707 200
Size: 731 776 3088
Fragments arranged by size:
3088 776 731
Bme1580I G_kGCm'C
Cuts at: 2220 2313 3369 2220
Size: 93 1056 3446
Fragments arranged by size:
3446 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1807 1888 2223 4367 1807
Size: 81 335 2144 2035
Fragments arranged by size:
2144 2035 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 804 2962 804
Size: 2158 2437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 239
Size: 4595
Bpu10I CC'TnA_GC
Cuts at: 354 354
Size: 4595
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 1771 2650 3142 3440 546
Size: 1225 879 492 298 1701
Fragments arranged by size:
1701 1225 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4299 4299
Size: 4595
BsaAI yAC'GTr
Cuts at: 1948 1948
Size: 4595
BsaBI GATnn'nnATC
Cuts at: 6 2109 4146 6
Size: 2103 2037 455
Fragments arranged by size:
2103 2037 455
BsaHI Gr'CG_yC
Cuts at: 2026 2274 2976 4472 2026
Size: 248 702 1496 2149
Fragments arranged by size:
2149 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1784 2305 2983 3257 3404 3815 1784
Size: 521 678 274 147 411 2564
Fragments arranged by size:
2564 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 780 780
Size: 4595
BseYI C'CCAG_C
Cuts at: 2020 2778 3355 3851 2020
Size: 758 577 496 2764
Fragments arranged by size:
2764 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2137 2183 3391 104
Size: 2033 46 1208 1308
Fragments arranged by size:
2033 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1609 2387 2577 3369 1609
Size: 778 190 792 2835
Fragments arranged by size:
2835 792 778 190
BsmI GAATG_Cn'
Cuts at: 462 1131 462
Size: 669 3926
Bsp1286I G_dGCh'C
Cuts at: 1609 2220 2313 2387 2577 2639 3369 1609
Size: 611 93 74 190 62 730 2835
Fragments arranged by size:
2835 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3815 3815
Size: 4595
BspHI T'CATG_A
Cuts at: 3771 3771
Size: 4595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 508 1645 1859 2161 2542 508
Size: 1137 214 302 381 2561
Fragments arranged by size:
2561 1137 381 302 214
BsrBI CCG'CTC
Cuts at: 50 114 2887 2941 4425 4590 50
Size: 64 2773 54 1484 165 55
Fragments arranged by size:
2773 1484 165 64 55 54
BsrDI GCAATG_nn'
Cuts at: 2507 2507
Size: 4595
BsrFI r'CCGG_y
Cuts at: 1061 2593 2774 2983 1061
Size: 1532 181 209 2673
Fragments arranged by size:
2673 1532 209 181
BsrGI T'GTAC_A
Cuts at: 877 1183 877
Size: 306 4289
BssSI C'ACGA_G
Cuts at: 2866 3224 2866
Size: 358 4237
BstAPI GCAn_nnn'nTGC
Cuts at: 642 1972 642
Size: 1330 3265
BstBI TT'CG_AA
Cuts at: 2956 2956
Size: 4595
BstXI CCAn_nnnn'nTGG
Cuts at: 1089 1089
Size: 4595
BstYI r'GATC_y
Cuts at: 1 1233 1620 2087 2110 2445 2691 3703 3789
Size: 1232 387 467 23 335 246 1012 86
Cuts at: 3789 3801 4147 1
Size: 12 346 449
Fragments arranged by size:
1232 1012 467 449 387 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 593 593
Size: 4595
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2545 2726 2889 2545
Size: 181 163 4251
Fragments arranged by size:
4251 181 163
BtsI GCAGTG_nn'
Cuts at: 1838 1838
Size: 4595
ClaI AT'CG_AT
Cuts at: 836 836
Size: 4595
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1332 1367 1332
Size: 35 4560
DraI TTT'AAA
Cuts at: 1594 1594
Size: 4595
DrdI GACnn_nn'nnGTC
Cuts at: 2301 3159 2301
Size: 858 3737
EaeI y'GGCC_r
Cuts at: 2180 2354 2745 2772 4117 2180
Size: 174 391 27 1345 2658
Fragments arranged by size:
2658 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2180 2180
Size: 4595
EarI CTCTTCn'nnn_
Cuts at: 344 1568 2618 2828 344
Size: 1224 1050 210 2111
Fragments arranged by size:
2111 1224 1050 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2803 3113 3259 4405 2803
Size: 310 146 1146 2993
Fragments arranged by size:
2993 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1607 1607
Size: 4595
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 518 533 908 910 973 1388 2420 2852
Size: 279 15 375 2 63 415 1032 432
Cuts at: 2852 3599 3867 239
Size: 747 268 967
Fragments arranged by size:
1032 967 747 432 415 375 279 268
63 15 2
EcoO109I rG'GnC_Cy
Cuts at: 1734 1734
Size: 4595
EcoRI G'AATT_C
Cuts at: 1599 1599
Size: 4595
EcoRV GAT'ATC
Cuts at: 1494 1494
Size: 4595
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 520 2614 520
Size: 2094 2501
FspI TGC'GCA
Cuts at: 2376 2376
Size: 4595
HaeII r_GCGC'y
Cuts at: 2029 2277 3299 4278 2029
Size: 248 1022 979 2346
Fragments arranged by size:
2346 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 778 810 1102 1134 1324 1356 2437 2467 2469
Size: 32 292 32 190 32 1081 30 2
Cuts at: 2469 2499 778
Size: 30 2874
Fragments arranged by size:
2874 1081 292 190 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 215 1634 215
Size: 1419 3176
KasI G'GCGC_C
Cuts at: 2025 2273 2025
Size: 248 4347
KpnI G_GTAC'C
Cuts at: 1615 1615
Size: 4595
MluI A'CGCG_T
Cuts at: 4028 4028
Size: 4595
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 697 1039 1145 3266 3450 697
Size: 342 106 2121 184 1842
Fragments arranged by size:
2121 1842 342 184 106
MscI TGG'CCA
Cuts at: 2356 2356
Size: 4595
MslI CAynn'nnrTG
Cuts at: 1087 2584 2711 1087
Size: 1497 127 2971
Fragments arranged by size:
2971 1497 127
MspA1I CmG'CkG
Cuts at: 204 1706 2020 2380 3393 3638 204
Size: 1502 314 360 1013 245 1161
Fragments arranged by size:
1502 1161 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1063 2776 1063
Size: 1713 2882
NarI GG'CG_CC
Cuts at: 2026 2274 2026
Size: 248 4347
NcoI C'CATG_G
Cuts at: 593 593
Size: 4595
NgoMIV G'CCGG_C
Cuts at: 1061 2774 1061
Size: 1713 2882
NspI r_CATG'y
Cuts at: 1648 2148 3055 1648
Size: 500 907 3188
Fragments arranged by size:
3188 907 500
PciI A'CATG_T
Cuts at: 3051 3051
Size: 4595
PmeI GTTT'AAAC
Cuts at: 1594 1594
Size: 4595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 3734
Size: 32 4563
PshAI GACnn'nnGTC
Cuts at: 220 220
Size: 4595
PsiI TTA'TAA
Cuts at: 1106 1106
Size: 4595
PstI C_TGCA'G
Cuts at: 570 638 1642 2327 570
Size: 68 1004 685 2838
Fragments arranged by size:
2838 1004 685 68
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4595
PvuII CAG'CTG
Cuts at: 204 2020 2380 204
Size: 1816 360 2419
Fragments arranged by size:
2419 1816 360
RsrII CG'GwC_CG
Cuts at: 2790 2790
Size: 4595
SacI G_AGCT'C
Cuts at: 1609 1609
Size: 4595
SalI G'TCGA_C
Cuts at: 1632 1632
Size: 4595
SapI GCTCTTCn'nnn_
Cuts at: 344 2618 2828 344
Size: 2274 210 2111
Fragments arranged by size:
2274 2111 210
SbfI CC_TGCA'GG
Cuts at: 1642 1642
Size: 4595
ScaI AGT'ACT
Cuts at: 1376 4112 1376
Size: 2736 1859
SfcI C'TryA_G
Cuts at: 33 566 634 1638 2323 3316 3507 33
Size: 533 68 1004 685 993 191 1121
Fragments arranged by size:
1121 1004 993 685 533 191 68
SfoI GGC'GCC
Cuts at: 2027 2275 2027
Size: 248 4347
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4595
SmaI CCC'GGG
Cuts at: 1617 1617
Size: 4595
SmlI C'TyrA_G
Cuts at: 525 1138 1750 2665 3157 3419 525
Size: 613 612 915 492 262 1701
Fragments arranged by size:
1701 915 613 612 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4595
SphI G_CATG'C
Cuts at: 1648 2148 1648
Size: 500 4095
StyI C'CwwG_G
Cuts at: 435 593 1729 2083 435
Size: 158 1136 354 2947
Fragments arranged by size:
2947 1136 354 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1413 2178 2311 2770 2979 1413
Size: 765 133 459 209 3029
Fragments arranged by size:
3029 765 459 209 133
TatI w'GTAC_w
Cuts at: 877 1183 1374 4110 877
Size: 306 191 2736 1362
Fragments arranged by size:
2736 1362 306 191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4112 4144 4112
Size: 32 4563
Tth111I GACn'n_nGTC
Cuts at: 1468 2392 1468
Size: 924 3671
XbaI T'CTAG_A
Cuts at: 1236 1626 1236
Size: 390 4205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2090 2090
Size: 4595
XmaI C'CCGG_G
Cuts at: 1615 1615
Size: 4595
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PshAI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI ApaI AscI AvrII
BaeI BfrBI BglI BmgBI BmtI BsaXI BsgI BsiWI
BsmBI BssHII BstEII BstZ17I Bsu36I DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI