Restriction map for
pF1KB4671
(Circular) (Six-base) MAPSORT of: pF1KB4671.seq Check: 4149 from: 1 to: 4049
pF1KB4671 4049 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 1087 1087
Size: 4049
Acc65I G'GTAC_C
Cuts at: 1065 1065
Size: 4049
AclI AA'CG_TT
Cuts at: 3889 3889
Size: 4049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1874 2306 3053 3321 1874
Size: 432 747 268 2602
Fragments arranged by size:
2602 747 432 268
AflIII A'CryG_T
Cuts at: 538 1401 2505 3482 538
Size: 863 1104 977 1105
Fragments arranged by size:
1105 1104 977 863
AgeI A'CCGG_T
Cuts at: 2437 2437
Size: 4049
AlwNI CAG_nnn'CTG
Cuts at: 1354 2921 1354
Size: 1567 2482
ApaLI G'TGCA_C
Cuts at: 2819 2819
Size: 4049
ApoI r'AATT_y
Cuts at: 808 1053 3402 3658 808
Size: 245 2349 256 1199
Fragments arranged by size:
2349 1199 256 245
AseI AT'TA_AT
Cuts at: 20 20
Size: 4049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 1069 1069
Size: 4049
BamHI G'GATC_C
Cuts at: 1074 1074
Size: 4049
BanI G'GyrC_C
Cuts at: 1065 1479 1727 1762 1065
Size: 414 248 35 3352
Fragments arranged by size:
3352 414 248 35
BanII G_rGCy'C
Cuts at: 1063 2093 1063
Size: 1030 3019
BbeI G_GCGC'C
Cuts at: 1483 1731 1483
Size: 248 3801
BbvCI CC'TCA_GC
Cuts at: 596 596
Size: 4049
BciVI GTATCCnnnnn_n'
Cuts at: 1947 2708 4049 1947
Size: 761 1341 1947
Fragments arranged by size:
1947 1341 761
BclI T'GATC_A
Cuts at: 252 366 1569 252
Size: 114 1203 2732
Fragments arranged by size:
2732 1203 114
BglII A'GATC_T
Cuts at: 684 1564 3601 684
Size: 880 2037 1132
Fragments arranged by size:
2037 1132 880
BlpI GC'TnA_GC
Cuts at: 1161 1161
Size: 4049
Bme1580I G_kGCm'C
Cuts at: 1674 1767 2823 1674
Size: 93 1056 2900
Fragments arranged by size:
2900 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1261 1342 1677 3821 1261
Size: 81 335 2144 1489
Fragments arranged by size:
2144 1489 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 435 435
Size: 4049
Bpu10I CC'TnA_GC
Cuts at: 305 596 305
Size: 291 3758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 1225 2104 2596 2894 859
Size: 366 879 492 298 2014
Fragments arranged by size:
2014 879 492 366 298
BsaI GGTCTCn'nnnn_
Cuts at: 3753 3753
Size: 4049
BsaAI yAC'GTr
Cuts at: 539 1402 539
Size: 863 3186
BsaBI GATnn'nnATC
Cuts at: 6 1563 3600 6
Size: 1557 2037 455
Fragments arranged by size:
2037 1557 455
BsaHI Gr'CG_yC
Cuts at: 1480 1728 2430 3926 1480
Size: 248 702 1496 1603
Fragments arranged by size:
1603 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1238 1759 2437 2711 2858 3269 1238
Size: 521 678 274 147 411 2018
Fragments arranged by size:
2018 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 280 310 410 440 967 997 280
Size: 30 100 30 527 30 3332
Fragments arranged by size:
3332 527 100 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 653 679 653
Size: 26 4023
BseYI C'CCAG_C
Cuts at: 1474 2232 2809 3305 1474
Size: 758 577 496 2218
Fragments arranged by size:
2218 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1591 1637 2845 104
Size: 1487 46 1208 1308
Fragments arranged by size:
1487 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 151 1063 1841 2031 2823 151
Size: 912 778 190 792 1377
Fragments arranged by size:
1377 912 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 151 1063 1674 1767 1841 2031 2093 2823 151
Size: 912 611 93 74 190 62 730 1377
Fragments arranged by size:
1377 912 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3269 3269
Size: 4049
BspHI T'CATG_A
Cuts at: 3225 3225
Size: 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1099 1313 1615 1996 1099
Size: 214 302 381 3152
Fragments arranged by size:
3152 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2341 2395 3879 4044 50
Size: 2291 54 1484 165 55
Fragments arranged by size:
2291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1961 1961
Size: 4049
BsrFI r'CCGG_y
Cuts at: 2047 2228 2437 2047
Size: 181 209 3659
Fragments arranged by size:
3659 209 181
BsrGI T'GTAC_A
Cuts at: 541 541
Size: 4049
BssSI C'ACGA_G
Cuts at: 622 2320 2678 622
Size: 1698 358 1993
Fragments arranged by size:
1993 1698 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1426 1426
Size: 4049
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstXI CCAn_nnnn'nTGG
Cuts at: 993 993
Size: 4049
BstYI r'GATC_y
Cuts at: 1 684 1074 1541 1564 1899 2145 3157 3243
Size: 683 390 467 23 335 246 1012 86
Cuts at: 3243 3255 3601 1
Size: 12 346 449
Fragments arranged by size:
1012 683 467 449 390 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4049
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1999 2180 2343 1999
Size: 181 163 3705
Fragments arranged by size:
3705 181 163
BtsI GCAGTG_nn'
Cuts at: 500 1292 500
Size: 792 3257
DraI TTT'AAA
Cuts at: 384 1048 384
Size: 664 3385
DrdI GACnn_nn'nnGTC
Cuts at: 1755 2613 1755
Size: 858 3191
EaeI y'GGCC_r
Cuts at: 390 1634 1808 2199 2226 3571 390
Size: 1244 174 391 27 1345 868
Fragments arranged by size:
1345 1244 868 391 174 27
EagI C'GGCC_G
Cuts at: 1634 1634
Size: 4049
EarI CTCTTCn'nnn_
Cuts at: 337 2072 2282 337
Size: 1735 210 2104
Fragments arranged by size:
2104 1735 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2257 2567 2713 3859 2257
Size: 310 146 1146 2447
Fragments arranged by size:
2447 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1061 1061
Size: 4049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 435 1874 2306 3053 3321 435
Size: 1439 432 747 268 1163
Fragments arranged by size:
1439 1163 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1188 1188
Size: 4049
EcoRI G'AATT_C
Cuts at: 1053 1053
Size: 4049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 4049
FspI TGC'GCA
Cuts at: 1830 1830
Size: 4049
HaeII r_GCGC'y
Cuts at: 1483 1731 2753 3732 1483
Size: 248 1022 979 1800
Fragments arranged by size:
1800 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 179 195 211 280 312 319 351 906
Size: 16 16 16 69 32 7 32 555
Cuts at: 906 938 967 999 1891 1921 1923 1953 163
Size: 32 29 32 892 30 2 30 2259
Fragments arranged by size:
2259 892 555 69 32 32 32 32
30 30 29 16 16 16 7 2
HincII GTy'rAC
Cuts at: 1088 1088
Size: 4049
KasI G'GCGC_C
Cuts at: 1479 1727 1479
Size: 248 3801
KpnI G_GTAC'C
Cuts at: 1069 1069
Size: 4049
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 135 624 2720 2904 135
Size: 489 2096 184 1280
Fragments arranged by size:
2096 1280 489 184
MscI TGG'CCA
Cuts at: 392 1810 392
Size: 1418 2631
MslI CAynn'nnrTG
Cuts at: 2038 2165 2038
Size: 127 3922
MspA1I CmG'CkG
Cuts at: 1160 1474 1834 2847 3092 1160
Size: 314 360 1013 245 2117
Fragments arranged by size:
2117 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 4049
NarI GG'CG_CC
Cuts at: 1480 1728 1480
Size: 248 3801
NcoI C'CATG_G
Cuts at: 107 107
Size: 4049
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 4049
NspI r_CATG'y
Cuts at: 830 841 1102 1602 2509 830
Size: 11 261 500 907 2370
Fragments arranged by size:
2370 907 500 261 11
PciI A'CATG_T
Cuts at: 2505 2505
Size: 4049
PfoI T'CCnGG_A
Cuts at: 414 414
Size: 4049
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 928 960 3188 3220 928
Size: 32 2228 32 1757
Fragments arranged by size:
2228 1757 32 32
PstI C_TGCA'G
Cuts at: 1096 1781 1096
Size: 685 3364
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4049
PvuII CAG'CTG
Cuts at: 1474 1834 1474
Size: 360 3689
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 1063 1063
Size: 4049
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SapI GCTCTTCn'nnn_
Cuts at: 337 2072 2282 337
Size: 1735 210 2104
Fragments arranged by size:
2104 1735 210
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 3566 3566
Size: 4049
SfcI C'TryA_G
Cuts at: 33 747 1092 1777 2770 2961 33
Size: 714 345 685 993 191 1121
Fragments arranged by size:
1121 993 714 685 345 191
SfoI GGC'GCC
Cuts at: 1481 1729 1481
Size: 248 3801
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 1071 1071
Size: 4049
SmlI C'TyrA_G
Cuts at: 874 1204 2119 2611 2873 874
Size: 330 915 492 262 2050
Fragments arranged by size:
2050 915 492 330 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4049
SphI G_CATG'C
Cuts at: 830 1102 1602 830
Size: 272 500 3277
Fragments arranged by size:
3277 500 272
StyI C'CwwG_G
Cuts at: 107 1183 1537 107
Size: 1076 354 2619
Fragments arranged by size:
2619 1076 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 562 1632 1765 2224 2433 562
Size: 1070 133 459 209 2178
Fragments arranged by size:
2178 1070 459 209 133
TatI w'GTAC_w
Cuts at: 541 3564 541
Size: 3023 1026
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 410 442 3566 3598 410
Size: 32 3124 32 861
Fragments arranged by size:
3124 861 32 32
Tth111I GACn'n_nGTC
Cuts at: 1846 1846
Size: 4049
XbaI T'CTAG_A
Cuts at: 613 1080 613
Size: 467 3582
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1544 1544
Size: 4049
XmaI C'CCGG_G
Cuts at: 1069 1069
Size: 4049
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI