Restriction map for
pF1KB4667
(Circular) (Six-base) MAPSORT of: pF1KB4667.seq Check: 1901 from: 1 to: 4178
pF1KB4667 4178 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 182 1090 182
Size: 908 3270
AatII G_ACGT'C
Cuts at: 1136 1136
Size: 4178
AccI GT'mk_AC
Cuts at: 1013 1216 1013
Size: 203 3975
Acc65I G'GTAC_C
Cuts at: 1194 1194
Size: 4178
AclI AA'CG_TT
Cuts at: 4018 4018
Size: 4178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 2003 2435 2788 3450 513
Size: 1490 432 353 662 1241
Fragments arranged by size:
1490 1241 662 432 353
AflIII A'CryG_T
Cuts at: 992 1530 3330 3611 992
Size: 538 1800 281 1559
Fragments arranged by size:
1800 1559 538 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 446 540 446
Size: 94 4084
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 658 690 658
Size: 32 4146
AlwNI CAG_nnn'CTG
Cuts at: 1483 2921 1483
Size: 1438 2740
ApaI G_GGCC'C
Cuts at: 369 445 369
Size: 76 4102
ApaLI G'TGCA_C
Cuts at: 3016 3016
Size: 4178
ApoI r'AATT_y
Cuts at: 1182 3531 3787 1182
Size: 2349 256 1573
Fragments arranged by size:
2349 1573 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4178
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
AvaI C'yCGr_G
Cuts at: 472 641 973 1198 472
Size: 169 332 225 3452
Fragments arranged by size:
3452 332 225 169
BamHI G'GATC_C
Cuts at: 743 1203 743
Size: 460 3718
BanI G'GyrC_C
Cuts at: 226 1194 1608 1856 1891 226
Size: 968 414 248 35 2513
Fragments arranged by size:
2513 968 414 248 35
BanII G_rGCy'C
Cuts at: 369 445 692 791 1192 2222 369
Size: 76 247 99 401 1030 2325
Fragments arranged by size:
2325 1030 401 247 99 76
BbeI G_GCGC'C
Cuts at: 1612 1860 1612
Size: 248 3930
BbsI GAAGACnn'nnnn_
Cuts at: 1040 1107 1040
Size: 67 4111
BciVI GTATCCnnnnn_n'
Cuts at: 2076 3132 4178 2076
Size: 1056 1046 2076
Fragments arranged by size:
2076 1056 1046
BclI T'GATC_A
Cuts at: 1698 1698
Size: 4178
BglII A'GATC_T
Cuts at: 1064 1693 3730 1064
Size: 629 2037 1512
Fragments arranged by size:
2037 1512 629
BlpI GC'TnA_GC
Cuts at: 790 1290 790
Size: 500 3678
Bme1580I G_kGCm'C
Cuts at: 229 369 445 519 1803 1896 3020 229
Size: 140 76 74 1284 93 1124 1387
Fragments arranged by size:
1387 1284 1124 140 93 76 74
BmgBI CAC'GTC
Cuts at: 711 711
Size: 4178
BmrI ACTGGGnnnn_n'
Cuts at: 1390 1471 1806 3950 1390
Size: 81 335 2144 1618
Fragments arranged by size:
2144 1618 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1086 2545 1086
Size: 1459 2719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 522 574 607 316
Size: 206 52 33 3887
Fragments arranged by size:
3887 206 52 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 1354 2233 2947 3245 1138
Size: 216 879 714 298 2071
Fragments arranged by size:
2071 879 714 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 1048 3882 1048
Size: 2834 1344
BsaAI yAC'GTr
Cuts at: 995 1531 995
Size: 536 3642
BsaBI GATnn'nnATC
Cuts at: 6 268 1692 3729 6
Size: 262 1424 2037 455
Fragments arranged by size:
2037 1424 455 262
BsaHI Gr'CG_yC
Cuts at: 761 1133 1609 1857 2559 4055 761
Size: 372 476 248 702 1496 884
Fragments arranged by size:
1496 884 702 476 372 248
BsaWI w'CCGG_w
Cuts at: 1367 1888 2566 2977 3124 3398 1367
Size: 521 678 411 147 274 2147
Fragments arranged by size:
2147 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 658 688 658
Size: 30 4148
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1015 1015
Size: 4178
BseYI C'CCAG_C
Cuts at: 222 480 1603 2361 3026 3434 222
Size: 258 1123 758 665 408 966
Fragments arranged by size:
1123 966 758 665 408 258
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 442
Size: 4178
BsiEI CG_ry'CG
Cuts at: 104 405 1720 1766 2996 104
Size: 301 1315 46 1230 1286
Fragments arranged by size:
1315 1286 1230 301 46
BsiHKAI G_wGCw'C
Cuts at: 250 692 791 1192 1970 2160 3020 250
Size: 442 99 401 778 190 860 1408
Fragments arranged by size:
1408 860 778 442 401 190 99
BsiWI C'GTAC_G
Cuts at: 995 995
Size: 4178
Bsp1286I G_dGCh'C
Cuts at: 229 250 369 445 519 692 791 1192 1803
Size: 21 119 76 74 173 99 401 611
Cuts at: 1803 1896 1970 2160 2222 3020 229
Size: 93 74 190 62 798 1387
Fragments arranged by size:
1387 798 611 401 190 173 119 99
93 76 74 74 62 21
BspHI T'CATG_A
Cuts at: 2610 2610
Size: 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 182 935 1090 1228 1442 1744 2125 182
Size: 753 155 138 214 302 381 2235
Fragments arranged by size:
2235 753 381 302 214 155 138
BsrBI CCG'CTC
Cuts at: 50 536 957 2470 2524 4008 4173 50
Size: 486 421 1513 54 1484 165 55
Fragments arranged by size:
1513 1484 486 421 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2090 2090
Size: 4178
BsrFI r'CCGG_y
Cuts at: 2176 2357 2176
Size: 181 3997
BssSI C'ACGA_G
Cuts at: 2449 3157 2449
Size: 708 3470
BstAPI GCAn_nnn'nTGC
Cuts at: 1555 1555
Size: 4178
BstBI TT'CG_AA
Cuts at: 2539 2539
Size: 4178
BstYI r'GATC_y
Cuts at: 1 743 941 1064 1203 1670 1693 2028 2274
Size: 742 198 123 139 467 23 335 246
Cuts at: 2274 2580 2592 2678 3730 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 742 467 449 335 306 246 198
139 123 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 379 379
Size: 4178
BtgI C'CryG_G
Cuts at: 107 653 107
Size: 546 3632
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 579 2128 2309 2472 579
Size: 1549 181 163 2285
Fragments arranged by size:
2285 1549 181 163
BtsI GCAGTG_nn'
Cuts at: 747 1421 747
Size: 674 3504
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 642 677 642
Size: 35 4143
DraI TTT'AAA
Cuts at: 1177 1177
Size: 4178
DrdI GACnn_nn'nnGTC
Cuts at: 1884 3228 1884
Size: 1344 2834
EaeI y'GGCC_r
Cuts at: 371 1763 1937 2328 2355 3700 371
Size: 1392 174 391 27 1345 849
Fragments arranged by size:
1392 1345 849 391 174 27
EagI C'GGCC_G
Cuts at: 1763 1763
Size: 4178
EarI CTCTTCn'nnn_
Cuts at: 913 2201 2411 913
Size: 1288 210 2680
Fragments arranged by size:
2680 1288 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 934 2386 3128 3274 3988 934
Size: 1452 742 146 714 1124
Fragments arranged by size:
1452 1124 742 714 146
EcoICRI GAG'CTC
Cuts at: 690 789 1190 690
Size: 99 401 3678
Fragments arranged by size:
3678 401 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 513 522 574 607 2003 2435 2788 3450
Size: 197 9 52 33 1396 432 353 662
Cuts at: 3450 316
Size: 1044
Fragments arranged by size:
1396 1044 662 432 353 197 52 33
9
EcoO109I rG'GnC_Cy
Cuts at: 366 442 924 1317 366
Size: 76 482 393 3227
Fragments arranged by size:
3227 482 393 76
EcoRI G'AATT_C
Cuts at: 1182 1182
Size: 4178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 696 2197 696
Size: 1501 2677
FspI TGC'GCA
Cuts at: 1959 1959
Size: 4178
HaeII r_GCGC'y
Cuts at: 1612 1860 3090 3861 1612
Size: 248 1230 771 1929
Fragments arranged by size:
1929 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2020 2050 2052 2082 2020
Size: 30 2 30 4116
Fragments arranged by size:
4116 30 30 2
HincII GTy'rAC
Cuts at: 1217 1217
Size: 4178
KasI G'GCGC_C
Cuts at: 1608 1856 1608
Size: 248 3930
KpnI G_GTAC'C
Cuts at: 1198 1198
Size: 4178
MluI A'CGCG_T
Cuts at: 3611 3611
Size: 4178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 814 2937 3121 814
Size: 2123 184 1871
Fragments arranged by size:
2123 1871 184
MscI TGG'CCA
Cuts at: 1939 1939
Size: 4178
MslI CAynn'nnrTG
Cuts at: 2167 2294 2167
Size: 127 4051
MspA1I CmG'CkG
Cuts at: 192 281 448 604 794 1009 1023 1289 1603
Size: 89 167 156 190 215 14 266 314
Cuts at: 1603 1963 2747 2992 192
Size: 360 784 245 1378
Fragments arranged by size:
1378 784 360 314 266 245 215 190
167 156 89 14
NaeI GCC'GGC
Cuts at: 2359 2359
Size: 4178
NarI GG'CG_CC
Cuts at: 1609 1857 1609
Size: 248 3930
NcoI C'CATG_G
Cuts at: 107 107
Size: 4178
NgoMIV G'CCGG_C
Cuts at: 2357 2357
Size: 4178
NspI r_CATG'y
Cuts at: 1231 1731 3334 1231
Size: 500 1603 2075
Fragments arranged by size:
2075 1603 500
PasI CC'CwG_GG
Cuts at: 511 511
Size: 4178
PciI A'CATG_T
Cuts at: 3330 3330
Size: 4178
PfoI T'CCnGG_A
Cuts at: 154 154
Size: 4178
PmeI GTTT'AAAC
Cuts at: 1177 1177
Size: 4178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 658 690 2624 2656 658
Size: 32 1934 32 2180
Fragments arranged by size:
2180 1934 32 32
PpuMI rG'GwC_Cy
Cuts at: 924 924
Size: 4178
PshAI GACnn'nnGTC
Cuts at: 1132 1132
Size: 4178
PspOMI G'GGCC_C
Cuts at: 365 441 365
Size: 76 4102
PstI C_TGCA'G
Cuts at: 1225 1910 1225
Size: 685 3493
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4178
PvuII CAG'CTG
Cuts at: 604 1603 1963 604
Size: 999 360 2819
Fragments arranged by size:
2819 999 360
RsrII CG'GwC_CG
Cuts at: 2373 2373
Size: 4178
SacI G_AGCT'C
Cuts at: 692 791 1192 692
Size: 99 401 3678
Fragments arranged by size:
3678 401 99
SalI G'TCGA_C
Cuts at: 1215 1215
Size: 4178
SapI GCTCTTCn'nnn_
Cuts at: 2201 2411 2201
Size: 210 3968
SbfI CC_TGCA'GG
Cuts at: 1225 1225
Size: 4178
ScaI AGT'ACT
Cuts at: 3695 3695
Size: 4178
SfcI C'TryA_G
Cuts at: 33 1221 1906 2874 3065 33
Size: 1188 685 968 191 1146
Fragments arranged by size:
1188 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1610 1858 1610
Size: 248 3930
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
SmaI CCC'GGG
Cuts at: 643 1200 643
Size: 557 3621
SmlI C'TyrA_G
Cuts at: 1153 1333 2248 2962 3224 1153
Size: 180 915 714 262 2107
Fragments arranged by size:
2107 915 714 262 180
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4178
SphI G_CATG'C
Cuts at: 1231 1731 1231
Size: 500 3678
StuI AGG'CCT
Cuts at: 301 523 301
Size: 222 3956
StyI C'CwwG_G
Cuts at: 107 1058 1312 1666 107
Size: 951 254 354 2619
Fragments arranged by size:
2619 951 354 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 472 1761 1894 2353 2562 472
Size: 1289 133 459 209 2088
Fragments arranged by size:
2088 1289 459 209 133
TatI w'GTAC_w
Cuts at: 311 977 1157 3693 311
Size: 666 180 2536 796
Fragments arranged by size:
2536 796 666 180
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4146
Tth111I GACn'n_nGTC
Cuts at: 1975 1975
Size: 4178
XbaI T'CTAG_A
Cuts at: 1209 1209
Size: 4178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 660 1673 660
Size: 1013 3165
XmaI C'CCGG_G
Cuts at: 641 1198 641
Size: 557 3621
XmnI GAAnn'nnTTC
Cuts at: 673 673
Size: 4178
ZraI GAC'GTC
Cuts at: 1134 1134
Size: 4178
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI Bpu10I BsmI BsmBI
BspEI BsrGI BssHII BstEII BstXI BstZ17I ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI