Restriction map for
pF1KB4662
(Circular) (Six-base) MAPSORT of: pF1KB4662.seq Check: 8037 from: 1 to: 4295
pF1KB4662 4295 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 578 1027 1333 578
Size: 449 306 3540
Fragments arranged by size:
3540 449 306
Acc65I G'GTAC_C
Cuts at: 1311 1311
Size: 4295
AclI AA'CG_TT
Cuts at: 4135 4135
Size: 4295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2120 2552 2905 3567 2120
Size: 432 353 662 2848
Fragments arranged by size:
2848 662 432 353
AflIII A'CryG_T
Cuts at: 1647 3447 3728 1647
Size: 1800 281 2214
Fragments arranged by size:
2214 1800 281
AleI CACnn'nnGTG
Cuts at: 244 244
Size: 4295
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 185 217 185
Size: 32 4263
AlwNI CAG_nnn'CTG
Cuts at: 804 1600 3038 804
Size: 796 1438 2061
Fragments arranged by size:
2061 1438 796
ApaLI G'TGCA_C
Cuts at: 3133 3133
Size: 4295
ApoI r'AATT_y
Cuts at: 994 1123 1299 3648 3904 994
Size: 129 176 2349 256 1385
Fragments arranged by size:
2349 1385 256 176 129
AseI AT'TA_AT
Cuts at: 20 20
Size: 4295
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
AvaI C'yCGr_G
Cuts at: 1315 1315
Size: 4295
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1222 1255 1222
Size: 33 4262
BamHI G'GATC_C
Cuts at: 1320 1320
Size: 4295
BanI G'GyrC_C
Cuts at: 148 1311 1725 1973 2008 148
Size: 1163 414 248 35 2435
Fragments arranged by size:
2435 1163 414 248 35
BanII G_rGCy'C
Cuts at: 1309 2339 1309
Size: 1030 3265
BbeI G_GCGC'C
Cuts at: 1729 1977 1729
Size: 248 4047
BbsI GAAGACnn'nnnn_
Cuts at: 225 1148 225
Size: 923 3372
BciVI GTATCCnnnnn_n'
Cuts at: 703 1046 2193 3249 4295 703
Size: 343 1147 1056 1046 703
Fragments arranged by size:
1147 1056 1046 703 343
BclI T'GATC_A
Cuts at: 917 1815 917
Size: 898 3397
BglI GCCn_nnn'nGGC
Cuts at: 529 529
Size: 4295
BglII A'GATC_T
Cuts at: 988 1810 3847 988
Size: 822 2037 1436
Fragments arranged by size:
2037 1436 822
BlpI GC'TnA_GC
Cuts at: 1407 1407
Size: 4295
Bme1580I G_kGCm'C
Cuts at: 1920 2013 3137 1920
Size: 93 1124 3078
Fragments arranged by size:
3078 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 604 1507 1588 1923 4067 604
Size: 903 81 335 2144 832
Fragments arranged by size:
2144 903 832 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 4295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 1040 605
Size: 435 3860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 1471 2350 3064 3362 536
Size: 935 879 714 298 1469
Fragments arranged by size:
1469 935 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3999 3999
Size: 4295
BsaAI yAC'GTr
Cuts at: 774 1648 774
Size: 874 3421
BsaBI GATnn'nnATC
Cuts at: 6 1809 3846 6
Size: 1803 2037 455
Fragments arranged by size:
2037 1803 455
BsaHI Gr'CG_yC
Cuts at: 1726 1974 2676 4172 1726
Size: 248 702 1496 1849
Fragments arranged by size:
1849 1496 702 248
BsaWI w'CCGG_w
Cuts at: 508 1484 2005 2683 3094 3241 3515 508
Size: 976 521 678 411 147 274 1288
Fragments arranged by size:
1288 976 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 426 456 578 608 923 953 426
Size: 30 122 30 315 30 3768
Fragments arranged by size:
3768 315 122 30 30 30
BseYI C'CCAG_C
Cuts at: 204 661 1720 2478 3143 3551 204
Size: 457 1059 758 665 408 948
Fragments arranged by size:
1059 948 758 665 457 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1202 1202
Size: 4295
BsiEI CG_ry'CG
Cuts at: 104 1837 1883 3113 104
Size: 1733 46 1230 1286
Fragments arranged by size:
1733 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1309 2087 2277 3137 1309
Size: 778 190 860 2467
Fragments arranged by size:
2467 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 1309 1920 2013 2087 2277 2339 3137 1309
Size: 611 93 74 190 62 798 2467
Fragments arranged by size:
2467 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1199 2727 1199
Size: 1528 2767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1345 1559 1861 2242 1345
Size: 214 302 381 3398
Fragments arranged by size:
3398 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2587 2641 4125 4290 50
Size: 2537 54 1484 165 55
Fragments arranged by size:
2537 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2207 2207
Size: 4295
BsrFI r'CCGG_y
Cuts at: 2293 2474 2293
Size: 181 4114
BssSI C'ACGA_G
Cuts at: 2566 3274 2566
Size: 708 3587
BstAPI GCAn_nnn'nTGC
Cuts at: 1672 1672
Size: 4295
BstBI TT'CG_AA
Cuts at: 2656 2656
Size: 4295
BstXI CCAn_nnnn'nTGG
Cuts at: 246 651 246
Size: 405 3890
BstYI r'GATC_y
Cuts at: 1 267 988 1130 1320 1787 1810 2145 2391
Size: 266 721 142 190 467 23 335 246
Cuts at: 2391 2697 2709 2795 3847 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 721 467 449 335 306 266 246
190 142 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4295
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2245 2426 2589 2245
Size: 181 163 3951
Fragments arranged by size:
3951 181 163
BtsI GCAGTG_nn'
Cuts at: 1538 1538
Size: 4295
DraI TTT'AAA
Cuts at: 1294 1294
Size: 4295
DraIII CAC_nnn'GTG
Cuts at: 1011 1011
Size: 4295
DrdI GACnn_nn'nnGTC
Cuts at: 2001 3345 2001
Size: 1344 2951
EaeI y'GGCC_r
Cuts at: 1880 2054 2445 2472 3817 1880
Size: 174 391 27 1345 2358
Fragments arranged by size:
2358 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1880 1880
Size: 4295
EarI CTCTTCn'nnn_
Cuts at: 2318 2528 2318
Size: 210 4085
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2503 3245 3391 4105 2503
Size: 742 146 714 2693
Fragments arranged by size:
2693 742 714 146
EcoICRI GAG'CTC
Cuts at: 1307 1307
Size: 4295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 1040 2120 2552 2905 3567 605
Size: 435 1080 432 353 662 1333
Fragments arranged by size:
1333 1080 662 435 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1434 1434
Size: 4295
EcoRI G'AATT_C
Cuts at: 994 1299 994
Size: 305 3990
EcoRV GAT'ATC
Cuts at: 682 1197 682
Size: 515 3780
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 4295
FspI TGC'GCA
Cuts at: 2076 2076
Size: 4295
HaeII r_GCGC'y
Cuts at: 1729 1977 3207 3978 1729
Size: 248 1230 771 2046
Fragments arranged by size:
2046 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 212 244 671 703 845 877 973 1005 2137
Size: 32 427 32 142 32 96 32 1132
Cuts at: 2137 2167 2169 2199 212
Size: 30 2 30 2308
Fragments arranged by size:
2308 1132 427 142 96 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 318 1334 318
Size: 1016 3279
HindIII A'AGCT_T
Cuts at: 896 896
Size: 4295
HpaI GTT'AAC
Cuts at: 318 318
Size: 4295
KasI G'GCGC_C
Cuts at: 1725 1973 1725
Size: 248 4047
KpnI G_GTAC'C
Cuts at: 1315 1315
Size: 4295
MluI A'CGCG_T
Cuts at: 3728 3728
Size: 4295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 195 1037 3054 3238 195
Size: 842 2017 184 1252
Fragments arranged by size:
2017 1252 842 184
MscI TGG'CCA
Cuts at: 2056 2056
Size: 4295
MslI CAynn'nnrTG
Cuts at: 244 2284 2411 244
Size: 2040 127 2128
Fragments arranged by size:
2128 2040 127
MspA1I CmG'CkG
Cuts at: 973 1406 1720 2080 2864 3109 973
Size: 433 314 360 784 245 2159
Fragments arranged by size:
2159 784 433 360 314 245
NaeI GCC'GGC
Cuts at: 2476 2476
Size: 4295
NarI GG'CG_CC
Cuts at: 1726 1974 1726
Size: 248 4047
NcoI C'CATG_G
Cuts at: 107 107
Size: 4295
NgoMIV G'CCGG_C
Cuts at: 2474 2474
Size: 4295
NspI r_CATG'y
Cuts at: 1348 1848 3451 1348
Size: 500 1603 2192
Fragments arranged by size:
2192 1603 500
PciI A'CATG_T
Cuts at: 3447 3447
Size: 4295
PmeI GTTT'AAAC
Cuts at: 1294 1294
Size: 4295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2741 2773 2741
Size: 32 4263
PstI C_TGCA'G
Cuts at: 1342 2027 1342
Size: 685 3610
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4295
PvuII CAG'CTG
Cuts at: 973 1720 2080 973
Size: 747 360 3188
Fragments arranged by size:
3188 747 360
RsrII CG'GwC_CG
Cuts at: 2490 2490
Size: 4295
SacI G_AGCT'C
Cuts at: 1309 1309
Size: 4295
SalI G'TCGA_C
Cuts at: 1332 1332
Size: 4295
SapI GCTCTTCn'nnn_
Cuts at: 2318 2528 2318
Size: 210 4085
SbfI CC_TGCA'GG
Cuts at: 1342 1342
Size: 4295
ScaI AGT'ACT
Cuts at: 3812 3812
Size: 4295
SfcI C'TryA_G
Cuts at: 33 1338 2023 2991 3182 33
Size: 1305 685 968 191 1146
Fragments arranged by size:
1305 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1727 1975 1727
Size: 248 4047
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
SmaI CCC'GGG
Cuts at: 1317 1317
Size: 4295
SmlI C'TyrA_G
Cuts at: 551 1450 2365 3079 3341 551
Size: 899 915 714 262 1505
Fragments arranged by size:
1505 915 899 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4295
SphI G_CATG'C
Cuts at: 1348 1848 1348
Size: 500 3795
SspI AAT'ATT
Cuts at: 786 786
Size: 4295
StyI C'CwwG_G
Cuts at: 107 1429 1783 107
Size: 1322 354 2619
Fragments arranged by size:
2619 1322 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1165 1878 2011 2470 2679 1165
Size: 713 133 459 209 2781
Fragments arranged by size:
2781 713 459 209 133
TatI w'GTAC_w
Cuts at: 3810 3810
Size: 4295
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 426 458 578 610 3812 3844 426
Size: 32 120 32 3202 32 877
Fragments arranged by size:
3202 877 120 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2092 2092
Size: 4295
XbaI T'CTAG_A
Cuts at: 1326 1326
Size: 4295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1790 1790
Size: 4295
XmaI C'CCGG_G
Cuts at: 1315 1315
Size: 4295
XmnI GAAnn'nnTTC
Cuts at: 743 743
Size: 4295
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI ApaI
AscI AvrII BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BseRI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoNI FseI FspAI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI