Restriction map for
pF1KB4651
(Circular) (Six-base) MAPSORT of: pF1KB4651.seq Check: 4068 from: 1 to: 3947
pF1KB4651 3947 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 197 197
Size: 3947
AccI GT'mk_AC
Cuts at: 246 985 246
Size: 739 3208
Acc65I G'GTAC_C
Cuts at: 963 963
Size: 3947
AclI AA'CG_TT
Cuts at: 3787 3787
Size: 3947
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1772 2204 2951 3219 1772
Size: 432 747 268 2500
Fragments arranged by size:
2500 747 432 268
AflIII A'CryG_T
Cuts at: 1299 2403 3380 1299
Size: 1104 977 1866
Fragments arranged by size:
1866 1104 977
AgeI A'CCGG_T
Cuts at: 2335 2335
Size: 3947
AlwNI CAG_nnn'CTG
Cuts at: 135 1252 2819 135
Size: 1117 1567 1263
Fragments arranged by size:
1567 1263 1117
ApaLI G'TGCA_C
Cuts at: 2717 2717
Size: 3947
ApoI r'AATT_y
Cuts at: 638 683 911 951 3300 3556 638
Size: 45 228 40 2349 256 1029
Fragments arranged by size:
2349 1029 256 228 45 40
AseI AT'TA_AT
Cuts at: 20 20
Size: 3947
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3947
AvaI C'yCGr_G
Cuts at: 967 967
Size: 3947
BamHI G'GATC_C
Cuts at: 972 972
Size: 3947
BanI G'GyrC_C
Cuts at: 963 1377 1625 1660 963
Size: 414 248 35 3250
Fragments arranged by size:
3250 414 248 35
BanII G_rGCy'C
Cuts at: 747 961 1991 747
Size: 214 1030 2703
Fragments arranged by size:
2703 1030 214
BbeI G_GCGC'C
Cuts at: 1381 1629 1381
Size: 248 3699
BbvCI CC'TCA_GC
Cuts at: 692 692
Size: 3947
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3913
BciVI GTATCCnnnnn_n'
Cuts at: 1845 2606 3947 1845
Size: 761 1341 1845
Fragments arranged by size:
1845 1341 761
BclI T'GATC_A
Cuts at: 1467 1467
Size: 3947
BglI GCCn_nnn'nGGC
Cuts at: 295 295
Size: 3947
BglII A'GATC_T
Cuts at: 1462 3499 1462
Size: 2037 1910
BlpI GC'TnA_GC
Cuts at: 1059 1059
Size: 3947
Bme1580I G_kGCm'C
Cuts at: 1572 1665 2721 1572
Size: 93 1056 2798
Fragments arranged by size:
2798 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1159 1240 1575 3719 1159
Size: 81 335 2144 1387
Fragments arranged by size:
2144 1387 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 3947
Bpu10I CC'TnA_GC
Cuts at: 692 692
Size: 3947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1123 2002 2494 2792 1123
Size: 879 492 298 2278
Fragments arranged by size:
2278 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 192 3651 192
Size: 3459 488
BsaAI yAC'GTr
Cuts at: 1300 1300
Size: 3947
BsaBI GATnn'nnATC
Cuts at: 6 1461 3498 6
Size: 1455 2037 455
Fragments arranged by size:
2037 1455 455
BsaHI Gr'CG_yC
Cuts at: 1378 1626 2328 3824 1378
Size: 248 702 1496 1501
Fragments arranged by size:
1501 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1136 1657 2335 2609 2756 3167 1136
Size: 521 678 274 147 411 1916
Fragments arranged by size:
1916 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 734 764 734
Size: 30 3917
BseYI C'CCAG_C
Cuts at: 149 254 587 1372 2130 2707 3203 149
Size: 105 333 785 758 577 496 893
Fragments arranged by size:
893 785 758 577 496 333 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 231
Size: 3947
BsiEI CG_ry'CG
Cuts at: 104 1489 1535 2743 104
Size: 1385 46 1208 1308
Fragments arranged by size:
1385 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 961 1739 1929 2721 961
Size: 778 190 792 2187
Fragments arranged by size:
2187 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 747 961 1572 1665 1739 1929 1991 2721 747
Size: 214 611 93 74 190 62 730 1973
Fragments arranged by size:
1973 730 611 214 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3167 3167
Size: 3947
BspHI T'CATG_A
Cuts at: 3123 3123
Size: 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 197 465 997 1211 1513 1894 197
Size: 268 532 214 302 381 2250
Fragments arranged by size:
2250 532 381 302 268 214
BsrBI CCG'CTC
Cuts at: 50 2239 2293 3777 3942 50
Size: 2189 54 1484 165 55
Fragments arranged by size:
2189 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 318 1859 318
Size: 1541 2406
BsrFI r'CCGG_y
Cuts at: 1945 2126 2335 1945
Size: 181 209 3557
Fragments arranged by size:
3557 209 181
BssSI C'ACGA_G
Cuts at: 2218 2576 2218
Size: 358 3589
BstAPI GCAn_nnn'nTGC
Cuts at: 1324 1324
Size: 3947
BstBI TT'CG_AA
Cuts at: 2308 2308
Size: 3947
BstYI r'GATC_y
Cuts at: 1 217 366 972 1439 1462 1797 2043 3055
Size: 216 149 606 467 23 335 246 1012
Cuts at: 3055 3141 3153 3499 1
Size: 86 12 346 449
Fragments arranged by size:
1012 606 467 449 346 335 246 216
149 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1897 2078 2241 1897
Size: 181 163 3603
Fragments arranged by size:
3603 181 163
BtsI GCAGTG_nn'
Cuts at: 467 1190 467
Size: 723 3224
DraI TTT'AAA
Cuts at: 644 864 946 644
Size: 220 82 3645
Fragments arranged by size:
3645 220 82
DrdI GACnn_nn'nnGTC
Cuts at: 1653 2511 1653
Size: 858 3089
EaeI y'GGCC_r
Cuts at: 1532 1706 2097 2124 3469 1532
Size: 174 391 27 1345 2010
Fragments arranged by size:
2010 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1532 1532
Size: 3947
EarI CTCTTCn'nnn_
Cuts at: 1970 2180 1970
Size: 210 3737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2155 2465 2611 3757 2155
Size: 310 146 1146 2345
Fragments arranged by size:
2345 1146 310 146
EcoICRI GAG'CTC
Cuts at: 959 959
Size: 3947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1772 2204 2951 3219 1772
Size: 432 747 268 2500
Fragments arranged by size:
2500 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1086 1086
Size: 3947
EcoRI G'AATT_C
Cuts at: 683 951 683
Size: 268 3679
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1966 1966
Size: 3947
FspI TGC'GCA
Cuts at: 1728 1728
Size: 3947
HaeII r_GCGC'y
Cuts at: 1381 1629 2651 3630 1381
Size: 248 1022 979 1698
Fragments arranged by size:
1698 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 504 536 654 686 703 734 735 766 1789
Size: 32 118 32 17 31 1 31 1023
Cuts at: 1789 1819 1821 1851 504
Size: 30 2 30 2600
Fragments arranged by size:
2600 1023 118 32 32 31 31 30
30 17 2 1
HincII GTy'rAC
Cuts at: 801 986 801
Size: 185 3762
HpaI GTT'AAC
Cuts at: 801 801
Size: 3947
KasI G'GCGC_C
Cuts at: 1377 1625 1377
Size: 248 3699
KpnI G_GTAC'C
Cuts at: 967 967
Size: 3947
MluI A'CGCG_T
Cuts at: 3380 3380
Size: 3947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2618 2802 2618
Size: 184 3763
MscI TGG'CCA
Cuts at: 1708 1708
Size: 3947
MslI CAynn'nnrTG
Cuts at: 207 1936 2063 207
Size: 1729 127 2091
Fragments arranged by size:
2091 1729 127
MspA1I CmG'CkG
Cuts at: 187 445 691 1058 1372 1732 2745 2990 187
Size: 258 246 367 314 360 1013 245 1144
Fragments arranged by size:
1144 1013 367 360 314 258 246 245
NaeI GCC'GGC
Cuts at: 2128 2128
Size: 3947
NarI GG'CG_CC
Cuts at: 1378 1626 1378
Size: 248 3699
NgoMIV G'CCGG_C
Cuts at: 2126 2126
Size: 3947
NspI r_CATG'y
Cuts at: 302 1000 1500 2407 302
Size: 698 500 907 1842
Fragments arranged by size:
1842 907 698 500
PciI A'CATG_T
Cuts at: 2403 2403
Size: 3947
PmeI GTTT'AAAC
Cuts at: 946 946
Size: 3947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3086 3118 3086
Size: 32 3915
PstI C_TGCA'G
Cuts at: 411 994 1679 411
Size: 583 685 2679
Fragments arranged by size:
2679 685 583
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3947
PvuII CAG'CTG
Cuts at: 187 445 691 1372 1732 187
Size: 258 246 681 360 2402
Fragments arranged by size:
2402 681 360 258 246
RsrII CG'GwC_CG
Cuts at: 2142 2142
Size: 3947
SacI G_AGCT'C
Cuts at: 961 961
Size: 3947
SalI G'TCGA_C
Cuts at: 984 984
Size: 3947
SapI GCTCTTCn'nnn_
Cuts at: 1970 2180 1970
Size: 210 3737
SbfI CC_TGCA'GG
Cuts at: 994 994
Size: 3947
ScaI AGT'ACT
Cuts at: 3464 3464
Size: 3947
SfcI C'TryA_G
Cuts at: 33 124 407 990 1675 2668 2859 33
Size: 91 283 583 685 993 191 1121
Fragments arranged by size:
1121 993 685 583 283 191 91
SfoI GGC'GCC
Cuts at: 1379 1627 1379
Size: 248 3699
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3947
SmaI CCC'GGG
Cuts at: 969 969
Size: 3947
SmlI C'TyrA_G
Cuts at: 1102 2017 2509 2771 1102
Size: 915 492 262 2278
Fragments arranged by size:
2278 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3947
SphI G_CATG'C
Cuts at: 1000 1500 1000
Size: 500 3447
SspI AAT'ATT
Cuts at: 889 889
Size: 3947
StuI AGG'CCT
Cuts at: 241 241
Size: 3947
StyI C'CwwG_G
Cuts at: 1081 1435 1081
Size: 354 3593
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 183 246 817 1530 1663 2122 2331 183
Size: 63 571 713 133 459 209 1799
Fragments arranged by size:
1799 713 571 459 209 133 63
TatI w'GTAC_w
Cuts at: 283 3462 283
Size: 3179 768
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3464 3496 3464
Size: 32 3915
Tth111I GACn'n_nGTC
Cuts at: 1744 1744
Size: 3947
XbaI T'CTAG_A
Cuts at: 978 978
Size: 3947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1442 1442
Size: 3947
XmaI C'CCGG_G
Cuts at: 967 967
Size: 3947
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BmgBI BmtI BpmI
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI