Restriction map for
pF1KB4636
(Circular) (Six-base) MAPSORT of: pF1KB4636.seq Check: 818 from: 1 to: 6491
pF1KB4636 6491 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 879 1160 1551 2100 879
Size: 281 391 549 5270
Fragments arranged by size:
5270 549 391 281
AccI GT'mk_AC
Cuts at: 3529 3529
Size: 6491
Acc65I G'GTAC_C
Cuts at: 1684 3507 1684
Size: 1823 4668
AclI AA'CG_TT
Cuts at: 6331 6331
Size: 6491
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 1888 2839 3083 4316 4748 5495 5763 307
Size: 1581 951 244 1233 432 747 268 1035
Fragments arranged by size:
1581 1233 1035 951 747 432 268 244
AfeI AGC'GCT
Cuts at: 976 3448 976
Size: 2472 4019
AflIII A'CryG_T
Cuts at: 3843 4947 5924 3843
Size: 1104 977 4410
Fragments arranged by size:
4410 1104 977
AgeI A'CCGG_T
Cuts at: 4879 4879
Size: 6491
AleI CACnn'nnGTG
Cuts at: 1436 1436
Size: 6491
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 263 3265 263
Size: 3002 3489
AlwNI CAG_nnn'CTG
Cuts at: 945 1657 1792 1920 1954 3016 3286 3796 5363
Size: 712 135 128 34 1062 270 510 1567
Cuts at: 5363 945
Size: 2073
Fragments arranged by size:
2073 1567 1062 712 510 270 135 128
34
ApaI G_GGCC'C
Cuts at: 213 674 865 1649 2331 2679 3265 213
Size: 461 191 784 682 348 586 3439
Fragments arranged by size:
3439 784 682 586 461 348 191
ApaLI G'TGCA_C
Cuts at: 5261 5261
Size: 6491
ApoI r'AATT_y
Cuts at: 2608 2783 3495 5844 6100 2608
Size: 175 712 2349 256 2999
Fragments arranged by size:
2999 2349 712 256 175
AseI AT'TA_AT
Cuts at: 20 20
Size: 6491
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6491
AvaI C'yCGr_G
Cuts at: 458 593 779 1410 1589 2243 3044 3077 3334
Size: 135 186 631 179 654 801 33 257
Cuts at: 3334 3511 458
Size: 177 3438
Fragments arranged by size:
3438 801 654 631 257 186 179 177
135 33
BamHI G'GATC_C
Cuts at: 442 2293 3516 442
Size: 1851 1223 3417
Fragments arranged by size:
3417 1851 1223
BanI G'GyrC_C
Cuts at: 1293 1563 1672 1684 1816 3360 3507 3921 4169
Size: 270 109 12 132 1544 147 414 248
Cuts at: 4169 4204 1293
Size: 35 3580
Fragments arranged by size:
3580 1544 414 270 248 147 132 109
35 12
BanII G_rGCy'C
Cuts at: 213 372 593 674 742 788 839 865 1371
Size: 159 221 81 68 46 51 26 506
Cuts at: 1371 1649 2233 2244 2331 2486 2679 3076 3265
Size: 278 584 11 87 155 193 397 189
Cuts at: 3265 3318 3334 3505 4535 213
Size: 53 16 171 1030 2169
Fragments arranged by size:
2169 1030 584 506 397 278 221 193
189 171 159 155 87 81 68 53
51 46 26 16 11
BbeI G_GCGC'C
Cuts at: 1676 3925 4173 1676
Size: 2249 248 3994
Fragments arranged by size:
3994 2249 248
BbsI GAAGACnn'nnnn_
Cuts at: 178 1328 3431 178
Size: 1150 2103 3238
Fragments arranged by size:
3238 2103 1150
BbvCI CC'TCA_GC
Cuts at: 423 1356 1361 2990 423
Size: 933 5 1629 3924
Fragments arranged by size:
3924 1629 933 5
BciVI GTATCCnnnnn_n'
Cuts at: 4389 5150 6491 4389
Size: 761 1341 4389
Fragments arranged by size:
4389 1341 761
BclI T'GATC_A
Cuts at: 623 1055 2522 4011 623
Size: 432 1467 1489 3103
Fragments arranged by size:
3103 1489 1467 432
BglI GCCn_nnn'nGGC
Cuts at: 1699 1944 3466 1699
Size: 245 1522 4724
Fragments arranged by size:
4724 1522 245
BglII A'GATC_T
Cuts at: 4006 6043 4006
Size: 2037 4454
BlpI GC'TnA_GC
Cuts at: 614 2215 3603 614
Size: 1601 1388 3502
Fragments arranged by size:
3502 1601 1388
Bme1580I G_kGCm'C
Cuts at: 213 674 819 865 1649 2331 2679 3265 4116
Size: 461 145 46 784 682 348 586 851
Cuts at: 4116 4209 5265 213
Size: 93 1056 1439
Fragments arranged by size:
1439 1056 851 784 682 586 461 348
145 93 46
BmrI ACTGGGnnnn_n'
Cuts at: 1645 2486 3703 3784 4119 6263 1645
Size: 841 1217 81 335 2144 1873
Fragments arranged by size:
2144 1873 1217 841 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3090 4858 3090
Size: 1768 4723
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 913 943 967 1183 1661 1976 2174 2336 2416
Size: 30 24 216 478 315 198 162 80
Cuts at: 2416 2707 2887 3376 913
Size: 291 180 489 4028
Fragments arranged by size:
4028 489 478 315 291 216 198 180
162 80 30 24
Bpu10I CC'TnA_GC
Cuts at: 423 1356 1361 1757 2984 2990 3302 3343 423
Size: 933 5 396 1227 6 312 41 3571
Fragments arranged by size:
3571 1227 933 396 312 41 6 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 756 2188 3667 4546 5038 5336 756
Size: 1432 1479 879 492 298 1911
Fragments arranged by size:
1911 1479 1432 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6195 6195
Size: 6491
BsaAI yAC'GTr
Cuts at: 3844 3844
Size: 6491
BsaBI GATnn'nnATC
Cuts at: 6 4005 6042 6
Size: 3999 2037 455
Fragments arranged by size:
3999 2037 455
BsaHI Gr'CG_yC
Cuts at: 1673 3922 4170 4872 6368 1673
Size: 2249 248 702 1496 1796
Fragments arranged by size:
2249 1796 1496 702 248
BsaWI w'CCGG_w
Cuts at: 485 1421 2181 2501 2594 3395 3680 4201 4879
Size: 936 760 320 93 801 285 521 678
Cuts at: 4879 5153 5300 5711 485
Size: 274 147 411 1265
Fragments arranged by size:
1265 936 801 760 678 521 411 320
285 274 147 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2043 2073 2414 2444 2043
Size: 30 341 30 6090
Fragments arranged by size:
6090 341 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 362 365 680 823 1054 1604 2066 2959
Size: 205 3 315 143 231 550 462 893
Cuts at: 2959 157
Size: 3689
Fragments arranged by size:
3689 893 550 462 315 231 205 143
3
BseYI C'CCAG_C
Cuts at: 819 849 855 1086 1865 2226 2438 2930 3009
Size: 30 6 231 779 361 212 492 79
Cuts at: 3009 3266 3443 3461 3916 4674 5251 5747 819
Size: 257 177 18 455 758 577 496 1563
Fragments arranged by size:
1563 779 758 577 496 492 455 361
257 231 212 177 79 30 18 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1119 2224 2587 2716 1119
Size: 1105 363 129 4894
Fragments arranged by size:
4894 1105 363 129
BsiEI CG_ry'CG
Cuts at: 104 4033 4079 5287 104
Size: 3929 46 1208 1308
Fragments arranged by size:
3929 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 242 742 839 929 1199 1512 2216 2873 3318
Size: 500 97 90 270 313 704 657 445
Cuts at: 3318 3505 4283 4473 5265 242
Size: 187 778 190 792 1468
Fragments arranged by size:
1468 792 778 704 657 500 445 313
270 190 187 97 90
BsmI GAATG_Cn'
Cuts at: 495 2719 3006 495
Size: 2224 287 3980
Fragments arranged by size:
3980 2224 287
BsmBI CGTCTCn'nnnn_
Cuts at: 3231 3231
Size: 6491
Bsp1286I G_dGCh'C
Cuts at: 213 242 372 593 674 742 788 819 839
Size: 29 130 221 81 68 46 31 20
Cuts at: 839 865 929 1199 1371 1512 1649 2216 2233
Size: 26 64 270 172 141 137 567 17
Cuts at: 2233 2244 2331 2486 2679 2873 3076 3265 3318
Size: 11 87 155 193 194 203 189 53
Cuts at: 3318 3334 3505 4116 4209 4283 4473 4535 5265
Size: 16 171 611 93 74 190 62 730
Cuts at: 5265 213
Size: 1439
Fragments arranged by size:
1439 730 611 567 270 221 203 194
193 190 189 172 171 155 141 137
130 93 87 81 74 68 64 62
53 46 31 29 26 20 17 16
11
BspEI T'CCGG_A
Cuts at: 1421 2501 2594 5711 1421
Size: 1080 93 3117 2201
Fragments arranged by size:
3117 2201 1080 93
BspHI T'CATG_A
Cuts at: 480 1454 5667 480
Size: 974 4213 1304
Fragments arranged by size:
4213 1304 974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 879 1124 1160 1551 1937 2100 2559 2738 3541
Size: 245 36 391 386 163 459 179 803
Cuts at: 3541 3755 4057 4438 879
Size: 214 302 381 2932
Fragments arranged by size:
2932 803 459 391 386 381 302 245
214 179 163 36
BsrBI CCG'CTC
Cuts at: 50 1715 1849 4783 4837 6321 6486 50
Size: 1665 134 2934 54 1484 165 55
Fragments arranged by size:
2934 1665 1484 165 134 55 54
BsrDI GCAATG_nn'
Cuts at: 242 2633 2652 4403 242
Size: 2391 19 1751 2330
Fragments arranged by size:
2391 2330 1751 19
BsrFI r'CCGG_y
Cuts at: 708 1675 4489 4670 4879 708
Size: 967 2814 181 209 2320
Fragments arranged by size:
2814 2320 967 209 181
BssSI C'ACGA_G
Cuts at: 1027 2798 4762 5120 1027
Size: 1771 1964 358 2398
Fragments arranged by size:
2398 1964 1771 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2112 2211 2649 3868 2112
Size: 99 438 1219 4735
Fragments arranged by size:
4735 1219 438 99
BstBI TT'CG_AA
Cuts at: 4852 4852
Size: 6491
BstXI CCAn_nnnn'nTGG
Cuts at: 1191 1989 2841 1191
Size: 798 852 4841
Fragments arranged by size:
4841 852 798
BstYI r'GATC_y
Cuts at: 1 442 2293 3516 3983 4006 4341 4587 5599
Size: 441 1851 1223 467 23 335 246 1012
Cuts at: 5599 5685 5697 6043 1
Size: 86 12 346 449
Fragments arranged by size:
1851 1223 1012 467 449 441 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2942 3065 2942
Size: 123 6368
BtgI C'CryG_G
Cuts at: 107 788 1007 1190 1325 1448 1892 2403 107
Size: 681 219 183 135 123 444 511 4195
Fragments arranged by size:
4195 681 511 444 219 183 135 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2008 4441 4622 4785 2008
Size: 2433 181 163 3714
Fragments arranged by size:
3714 2433 181 163
BtsI GCAGTG_nn'
Cuts at: 1663 1796 2262 2733 3734 1663
Size: 133 466 471 1001 4420
Fragments arranged by size:
4420 1001 471 466 133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1574 1609 1574
Size: 35 6456
DraI TTT'AAA
Cuts at: 3490 3490
Size: 6491
DraIII CAC_nnn'GTG
Cuts at: 360 360
Size: 6491
DrdI GACnn_nn'nnGTC
Cuts at: 1535 4197 5055 1535
Size: 2662 858 2971
Fragments arranged by size:
2971 2662 858
EaeI y'GGCC_r
Cuts at: 1542 2778 4076 4250 4641 4668 6013 1542
Size: 1236 1298 174 391 27 1345 2020
Fragments arranged by size:
2020 1345 1298 1236 391 174 27
EagI C'GGCC_G
Cuts at: 4076 4076
Size: 6491
EarI CTCTTCn'nnn_
Cuts at: 2363 2531 3026 4514 4724 2363
Size: 168 495 1488 210 4130
Fragments arranged by size:
4130 1488 495 210 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4699 5009 5155 6301 4699
Size: 310 146 1146 4889
Fragments arranged by size:
4889 1146 310 146
EcoICRI GAG'CTC
Cuts at: 740 837 3316 3503 740
Size: 97 2479 187 3728
Fragments arranged by size:
3728 2479 187 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 913 943 967 1183 1661 1888 1976 2174
Size: 606 30 24 216 478 227 88 198
Cuts at: 2174 2336 2416 2707 2839 2887 3083 3376 4316
Size: 162 80 291 132 48 196 293 940
Cuts at: 4316 4748 5495 5763 307
Size: 432 747 268 1035
Fragments arranged by size:
1035 940 747 606 478 432 293 291
268 227 216 198 196 162 132 88
80 48 30 24
EcoO109I rG'GnC_Cy
Cuts at: 209 463 470 573 670 671 1528 1645 1646
Size: 254 7 103 97 1 857 117 1
Cuts at: 1646 1763 1858 1936 2327 2328 2345 2675 2884
Size: 117 95 78 391 1 17 330 209
Cuts at: 2884 3144 3261 3262 3351 3630 209
Size: 260 117 1 89 279 3070
Fragments arranged by size:
3070 857 391 330 279 260 254 209
117 117 117 103 97 95 89 78
17 7 1 1 1 1
EcoRI G'AATT_C
Cuts at: 2783 3495 2783
Size: 712 5779
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4510 4510
Size: 6491
FspI TGC'GCA
Cuts at: 2642 4272 2642
Size: 1630 4861
HaeII r_GCGC'y
Cuts at: 978 1676 3450 3925 4173 5195 6174 978
Size: 698 1774 475 248 1022 979 1295
Fragments arranged by size:
1774 1295 1022 979 698 475 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 645 677 2043 2075 2161 2193 3025 3057 4333
Size: 32 1366 32 86 32 832 32 1276
Cuts at: 4333 4363 4365 4395 645
Size: 30 2 30 2741
Fragments arranged by size:
2741 1366 1276 832 86 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3530 3530
Size: 6491
HindIII A'AGCT_T
Cuts at: 319 319
Size: 6491
KasI G'GCGC_C
Cuts at: 1672 3921 4169 1672
Size: 2249 248 3994
Fragments arranged by size:
3994 2249 248
KpnI G_GTAC'C
Cuts at: 1688 3511 1688
Size: 1823 4668
MluI A'CGCG_T
Cuts at: 5924 5924
Size: 6491
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 492 1609 5162 5346 492
Size: 1117 3553 184 1637
Fragments arranged by size:
3553 1637 1117 184
MscI TGG'CCA
Cuts at: 1544 4252 1544
Size: 2708 3783
MslI CAynn'nnrTG
Cuts at: 562 1154 1189 1436 1987 2503 2839 4480 4607
Size: 592 35 247 551 516 336 1641 127
Cuts at: 4607 562
Size: 2446
Fragments arranged by size:
2446 1641 592 551 516 336 247 127
35
MspA1I CmG'CkG
Cuts at: 136 859 942 1050 1360 1840 2442 2994 3103
Size: 723 83 108 310 480 602 552 109
Cuts at: 3103 3124 3177 3270 3465 3602 3916 4276 5289
Size: 21 53 93 195 137 314 360 1013
Cuts at: 5289 5534 136
Size: 245 1093
Fragments arranged by size:
1093 1013 723 602 552 480 360 314
310 245 195 137 109 108 93 83
53 21
NaeI GCC'GGC
Cuts at: 4672 4672
Size: 6491
NarI GG'CG_CC
Cuts at: 1673 3922 4170 1673
Size: 2249 248 3994
Fragments arranged by size:
3994 2249 248
NcoI C'CATG_G
Cuts at: 107 788 1190 1892 2403 107
Size: 681 402 702 511 4195
Fragments arranged by size:
4195 702 681 511 402
NgoMIV G'CCGG_C
Cuts at: 4670 4670
Size: 6491
NspI r_CATG'y
Cuts at: 1159 2647 2877 3544 4044 4951 1159
Size: 1488 230 667 500 907 2699
Fragments arranged by size:
2699 1488 907 667 500 230
PasI CC'CwG_GG
Cuts at: 1524 1551 2235 1524
Size: 27 684 5780
Fragments arranged by size:
5780 684 27
PciI A'CATG_T
Cuts at: 4947 4947
Size: 6491
PflMI CCAn_nnn'nTGG
Cuts at: 360 1440 1551 1657 1954 3016 360
Size: 1080 111 106 297 1062 3835
Fragments arranged by size:
3835 1080 1062 297 111 106
PfoI T'CCnGG_A
Cuts at: 649 929 1638 2151 649
Size: 280 709 513 4989
Fragments arranged by size:
4989 709 513 280
PmeI GTTT'AAAC
Cuts at: 3490 3490
Size: 6491
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5630 5662 5630
Size: 32 6459
PpuMI rG'GwC_Cy
Cuts at: 1528 1763 3144 3351 1528
Size: 235 1381 207 4668
Fragments arranged by size:
4668 1381 235 207
PspOMI G'GGCC_C
Cuts at: 209 670 861 1645 2327 2675 3261 209
Size: 461 191 784 682 348 586 3439
Fragments arranged by size:
3439 784 682 586 461 348 191
PstI C_TGCA'G
Cuts at: 1670 1944 3129 3422 3538 4223 1670
Size: 274 1185 293 116 685 3938
Fragments arranged by size:
3938 1185 685 293 274 116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6491
PvuII CAG'CTG
Cuts at: 1360 1840 2442 3103 3124 3270 3916 4276 1360
Size: 480 602 661 21 146 646 360 3575
Fragments arranged by size:
3575 661 646 602 480 360 146 21
RsrII CG'GwC_CG
Cuts at: 4686 4686
Size: 6491
SacI G_AGCT'C
Cuts at: 742 839 3318 3505 742
Size: 97 2479 187 3728
Fragments arranged by size:
3728 2479 187 97
SalI G'TCGA_C
Cuts at: 3528 3528
Size: 6491
SanDI GG'GwC_CC
Cuts at: 1528 1763 3144 1528
Size: 235 1381 4875
Fragments arranged by size:
4875 1381 235
SapI GCTCTTCn'nnn_
Cuts at: 4514 4724 4514
Size: 210 6281
SbfI CC_TGCA'GG
Cuts at: 1944 3538 1944
Size: 1594 4897
ScaI AGT'ACT
Cuts at: 6008 6008
Size: 6491
SexAI A'CCwGG_T
Cuts at: 356 356
Size: 6491
SfcI C'TryA_G
Cuts at: 33 937 1666 1940 2879 3125 3381 3418 3435
Size: 904 729 274 939 246 256 37 17
Cuts at: 3435 3534 4219 5212 5403 33
Size: 99 685 993 191 1121
Fragments arranged by size:
1121 993 939 904 729 685 274 256
246 191 99 37 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 1944 1944
Size: 6491
SfoI GGC'GCC
Cuts at: 1674 3923 4171 1674
Size: 2249 248 3994
Fragments arranged by size:
3994 2249 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6491
SmaI CCC'GGG
Cuts at: 595 781 1591 3079 3513 595
Size: 186 810 1488 434 3573
Fragments arranged by size:
3573 1488 810 434 186
SmlI C'TyrA_G
Cuts at: 735 2167 3646 4561 5053 5315 735
Size: 1432 1479 915 492 262 1911
Fragments arranged by size:
1911 1479 1432 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6491
SphI G_CATG'C
Cuts at: 3544 4044 3544
Size: 500 5991
StuI AGG'CCT
Cuts at: 1318 2159 2705 1318
Size: 841 546 5104
Fragments arranged by size:
5104 841 546
StyI C'CwwG_G
Cuts at: 107 176 788 1190 1892 2019 2331 2403 2561
Size: 69 612 402 702 127 312 72 158
Cuts at: 2561 2700 2816 3115 3625 3979 107
Size: 139 116 299 510 354 2619
Fragments arranged by size:
2619 702 612 510 402 354 312 299
158 139 127 116 72 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 906 982 1890 2461 2505 4074 4207 4666 4875
Size: 76 908 571 44 1569 133 459 209
Cuts at: 4875 906
Size: 2522
Fragments arranged by size:
2522 1569 908 571 459 209 133 76
44
TatI w'GTAC_w
Cuts at: 6006 6006
Size: 6491
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1226 1258 1547 1579 6008 6040 1226
Size: 32 289 32 4429 32 1677
Fragments arranged by size:
4429 1677 289 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 414 4288 414
Size: 3874 2617
XbaI T'CTAG_A
Cuts at: 3522 3522
Size: 6491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 135 1521 1695 1986 3986 135
Size: 1386 174 291 2000 2640
Fragments arranged by size:
2640 2000 1386 291 174
XmaI C'CCGG_G
Cuts at: 593 779 1589 3077 3511 593
Size: 186 810 1488 434 3573
Fragments arranged by size:
3573 1488 810 434 186
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BcgI
BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I
ClaI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI