Restriction map for
pF1KB4616
(Circular) (Six-base) MAPSORT of: pF1KB4616.seq Check: 566 from: 1 to: 4145
pF1KB4616 4145 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 1183 1183
Size: 4145
Acc65I G'GTAC_C
Cuts at: 1161 1161
Size: 4145
AclI AA'CG_TT
Cuts at: 3985 3985
Size: 4145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1970 2402 3149 3417 817
Size: 1153 432 747 268 1545
Fragments arranged by size:
1545 1153 747 432 268
AflIII A'CryG_T
Cuts at: 157 1497 2601 3578 157
Size: 1340 1104 977 724
Fragments arranged by size:
1340 1104 977 724
AgeI A'CCGG_T
Cuts at: 2533 2533
Size: 4145
AlwNI CAG_nnn'CTG
Cuts at: 1450 3017 1450
Size: 1567 2578
ApaLI G'TGCA_C
Cuts at: 2915 2915
Size: 4145
ApoI r'AATT_y
Cuts at: 592 892 1149 3498 3754 592
Size: 300 257 2349 256 983
Fragments arranged by size:
2349 983 300 257 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4145
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
AvaI C'yCGr_G
Cuts at: 860 1165 860
Size: 305 3840
BamHI G'GATC_C
Cuts at: 1170 1170
Size: 4145
BanI G'GyrC_C
Cuts at: 1117 1161 1575 1823 1858 1117
Size: 44 414 248 35 3404
Fragments arranged by size:
3404 414 248 44 35
BanII G_rGCy'C
Cuts at: 176 726 844 1159 2189 176
Size: 550 118 315 1030 2132
Fragments arranged by size:
2132 1030 550 315 118
BbeI G_GCGC'C
Cuts at: 1579 1827 1579
Size: 248 3897
BbsI GAAGACnn'nnnn_
Cuts at: 494 542 494
Size: 48 4097
BciVI GTATCCnnnnn_n'
Cuts at: 2043 2804 4145 2043
Size: 761 1341 2043
Fragments arranged by size:
2043 1341 761
BclI T'GATC_A
Cuts at: 1665 1665
Size: 4145
BglII A'GATC_T
Cuts at: 1660 3697 1660
Size: 2037 2108
BlpI GC'TnA_GC
Cuts at: 1079 1257 1079
Size: 178 3967
Bme1580I G_kGCm'C
Cuts at: 1770 1863 2919 1770
Size: 93 1056 2996
Fragments arranged by size:
2996 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1106 1357 1438 1773 3917 1106
Size: 251 81 335 2144 1334
Fragments arranged by size:
2144 1334 335 251 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 4145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 137
Size: 4145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1321 2200 2692 2990 1321
Size: 879 492 298 2476
Fragments arranged by size:
2476 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3849 3849
Size: 4145
BsaAI yAC'GTr
Cuts at: 158 1498 158
Size: 1340 2805
BsaBI GATnn'nnATC
Cuts at: 6 1659 3696 6
Size: 1653 2037 455
Fragments arranged by size:
2037 1653 455
BsaHI Gr'CG_yC
Cuts at: 1576 1824 2526 4022 1576
Size: 248 702 1496 1699
Fragments arranged by size:
1699 1496 702 248
BsaWI w'CCGG_w
Cuts at: 645 1334 1855 2533 2807 2954 3365 645
Size: 689 521 678 274 147 411 1425
Fragments arranged by size:
1425 689 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 727 757 727
Size: 30 4115
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 490 940 490
Size: 450 3695
BseYI C'CCAG_C
Cuts at: 1570 2328 2905 3401 1570
Size: 758 577 496 2314
Fragments arranged by size:
2314 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1687 1733 2941 104
Size: 1583 46 1208 1308
Fragments arranged by size:
1583 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 844 1159 1937 2127 2919 844
Size: 315 778 190 792 2070
Fragments arranged by size:
2070 792 778 315 190
BsmI GAATG_Cn'
Cuts at: 217 217
Size: 4145
Bsp1286I G_dGCh'C
Cuts at: 176 726 844 1159 1770 1863 1937 2127 2189
Size: 550 118 315 611 93 74 190 62
Cuts at: 2189 2919 176
Size: 730 1402
Fragments arranged by size:
1402 730 611 550 315 190 118 93
74 62
BspEI T'CCGG_A
Cuts at: 645 3365 645
Size: 2720 1425
BspHI T'CATG_A
Cuts at: 3321 3321
Size: 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1195 1409 1711 2092 1195
Size: 214 302 381 3248
Fragments arranged by size:
3248 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2437 2491 3975 4140 50
Size: 2387 54 1484 165 55
Fragments arranged by size:
2387 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 971 2057 971
Size: 1086 3059
BsrFI r'CCGG_y
Cuts at: 2143 2324 2533 2143
Size: 181 209 3755
Fragments arranged by size:
3755 209 181
BssSI C'ACGA_G
Cuts at: 2416 2774 2416
Size: 358 3787
BstAPI GCAn_nnn'nTGC
Cuts at: 1522 1522
Size: 4145
BstBI TT'CG_AA
Cuts at: 2506 2506
Size: 4145
BstYI r'GATC_y
Cuts at: 1 1170 1637 1660 1995 2241 3253 3339 3351
Size: 1169 467 23 335 246 1012 86 12
Cuts at: 3351 3697 1
Size: 346 449
Fragments arranged by size:
1169 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 291 651 107
Size: 184 360 3601
Fragments arranged by size:
3601 360 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 485 1112 2095 2276 2439 485
Size: 627 983 181 163 2191
Fragments arranged by size:
2191 983 627 181 163
BtsI GCAGTG_nn'
Cuts at: 1388 1388
Size: 4145
DraI TTT'AAA
Cuts at: 1144 1144
Size: 4145
DrdI GACnn_nn'nnGTC
Cuts at: 1851 2709 1851
Size: 858 3287
EaeI y'GGCC_r
Cuts at: 278 629 968 1730 1904 2295 2322 3667 278
Size: 351 339 762 174 391 27 1345 756
Fragments arranged by size:
1345 762 756 391 351 339 174 27
EagI C'GGCC_G
Cuts at: 1730 1730
Size: 4145
EarI CTCTTCn'nnn_
Cuts at: 392 410 428 446 1124 2168 2378 392
Size: 18 18 18 678 1044 210 2159
Fragments arranged by size:
2159 1044 678 210 18 18 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 580 2353 2663 2809 3955 580
Size: 1773 310 146 1146 770
Fragments arranged by size:
1773 1146 770 310 146
EcoICRI GAG'CTC
Cuts at: 842 1157 842
Size: 315 3830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 817 1970 2402 3149 3417 137
Size: 680 1153 432 747 268 865
Fragments arranged by size:
1153 865 747 680 432 268
EcoO109I rG'GnC_Cy
Cuts at: 936 1108 1284 936
Size: 172 176 3797
Fragments arranged by size:
3797 176 172
EcoRI G'AATT_C
Cuts at: 1149 1149
Size: 4145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 4145
FspI TGC'GCA
Cuts at: 1926 1926
Size: 4145
HaeII r_GCGC'y
Cuts at: 1579 1827 2849 3828 1579
Size: 248 1022 979 1896
Fragments arranged by size:
1896 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 391 409 423 441 627 659 991 1023 1987
Size: 18 14 18 186 32 332 32 964
Cuts at: 1987 2017 2019 2049 391
Size: 30 2 30 2487
Fragments arranged by size:
2487 964 332 186 32 32 30 30
18 18 14 2
HincII GTy'rAC
Cuts at: 1184 1184
Size: 4145
KasI G'GCGC_C
Cuts at: 1575 1823 1575
Size: 248 3897
KpnI G_GTAC'C
Cuts at: 1165 1165
Size: 4145
MluI A'CGCG_T
Cuts at: 3578 3578
Size: 4145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 389 2816 3000 389
Size: 2427 184 1534
Fragments arranged by size:
2427 1534 184
MscI TGG'CCA
Cuts at: 631 970 1906 631
Size: 339 936 2870
Fragments arranged by size:
2870 936 339
MslI CAynn'nnrTG
Cuts at: 2134 2261 2134
Size: 127 4018
MspA1I CmG'CkG
Cuts at: 319 1078 1256 1570 1930 2943 3188 319
Size: 759 178 314 360 1013 245 1276
Fragments arranged by size:
1276 1013 759 360 314 245 178
NaeI GCC'GGC
Cuts at: 2326 2326
Size: 4145
NarI GG'CG_CC
Cuts at: 1576 1824 1576
Size: 248 3897
NcoI C'CATG_G
Cuts at: 107 651 107
Size: 544 3601
NgoMIV G'CCGG_C
Cuts at: 2324 2324
Size: 4145
NspI r_CATG'y
Cuts at: 1198 1698 2605 1198
Size: 500 907 2738
Fragments arranged by size:
2738 907 500
PciI A'CATG_T
Cuts at: 2601 2601
Size: 4145
PflMI CCAn_nnn'nTGG
Cuts at: 718 718
Size: 4145
PmeI GTTT'AAAC
Cuts at: 1144 1144
Size: 4145
PmlI CAC'GTG
Cuts at: 158 158
Size: 4145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 4113
PpuMI rG'GwC_Cy
Cuts at: 1108 1108
Size: 4145
PstI C_TGCA'G
Cuts at: 1192 1877 1192
Size: 685 3460
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4145
PvuII CAG'CTG
Cuts at: 319 1078 1570 1930 319
Size: 759 492 360 2534
Fragments arranged by size:
2534 759 492 360
RsrII CG'GwC_CG
Cuts at: 2340 2340
Size: 4145
SacI G_AGCT'C
Cuts at: 844 1159 844
Size: 315 3830
SalI G'TCGA_C
Cuts at: 1182 1182
Size: 4145
SapI GCTCTTCn'nnn_
Cuts at: 2168 2378 2168
Size: 210 3935
SbfI CC_TGCA'GG
Cuts at: 1192 1192
Size: 4145
ScaI AGT'ACT
Cuts at: 3662 3662
Size: 4145
SfcI C'TryA_G
Cuts at: 33 1188 1873 2866 3057 33
Size: 1155 685 993 191 1121
Fragments arranged by size:
1155 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1577 1825 1577
Size: 248 3897
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
SmaI CCC'GGG
Cuts at: 1167 1167
Size: 4145
SmlI C'TyrA_G
Cuts at: 1300 2215 2707 2969 1300
Size: 915 492 262 2476
Fragments arranged by size:
2476 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4145
SphI G_CATG'C
Cuts at: 1198 1698 1198
Size: 500 3645
StuI AGG'CCT
Cuts at: 304 304
Size: 4145
StyI C'CwwG_G
Cuts at: 107 651 1046 1279 1633 107
Size: 544 395 233 354 2619
Fragments arranged by size:
2619 544 395 354 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1728 1861 2320 2529 1728
Size: 133 459 209 3344
Fragments arranged by size:
3344 459 209 133
TatI w'GTAC_w
Cuts at: 3660 3660
Size: 4145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 727 759 3662 3694 727
Size: 32 2903 32 1178
Fragments arranged by size:
2903 1178 32 32
Tth111I GACn'n_nGTC
Cuts at: 1942 1942
Size: 4145
XbaI T'CTAG_A
Cuts at: 1176 1176
Size: 4145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1640 1640
Size: 4145
XmaI C'CCGG_G
Cuts at: 1165 1165
Size: 4145
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI