Restriction map for
pF1KB4609
(Circular) (Six-base) MAPSORT of: pF1KB4609.seq Check: 7351 from: 1 to: 3947
pF1KB4609 3947 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 985 985
Size: 3947
Acc65I G'GTAC_C
Cuts at: 963 963
Size: 3947
AclI AA'CG_TT
Cuts at: 3787 3787
Size: 3947
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 669 894 1772 2204 2557 3219 412
Size: 257 225 878 432 353 662 1140
Fragments arranged by size:
1140 878 662 432 353 257 225
AfeI AGC'GCT
Cuts at: 200 532 200
Size: 332 3615
AflIII A'CryG_T
Cuts at: 1299 3099 3380 1299
Size: 1800 281 1866
Fragments arranged by size:
1866 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 157 157
Size: 3947
AleI CACnn'nnGTG
Cuts at: 166 166
Size: 3947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 808 808
Size: 3947
AlwNI CAG_nnn'CTG
Cuts at: 272 594 1252 2690 272
Size: 322 658 1438 1529
Fragments arranged by size:
1529 1438 658 322
ApaLI G'TGCA_C
Cuts at: 697 727 2785 697
Size: 30 2058 1859
Fragments arranged by size:
2058 1859 30
ApoI r'AATT_y
Cuts at: 951 3300 3556 951
Size: 2349 256 1342
Fragments arranged by size:
2349 1342 256
AseI AT'TA_AT
Cuts at: 20 377 20
Size: 357 3590
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3947
AvaI C'yCGr_G
Cuts at: 967 967
Size: 3947
BamHI G'GATC_C
Cuts at: 306 972 306
Size: 666 3281
BanI G'GyrC_C
Cuts at: 514 963 1377 1625 1660 514
Size: 449 414 248 35 2801
Fragments arranged by size:
2801 449 414 248 35
BanII G_rGCy'C
Cuts at: 245 961 1991 245
Size: 716 1030 2201
Fragments arranged by size:
2201 1030 716
BbeI G_GCGC'C
Cuts at: 518 1381 1629 518
Size: 863 248 2836
Fragments arranged by size:
2836 863 248
BbvCI CC'TCA_GC
Cuts at: 324 324
Size: 3947
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 873 907 90
Size: 34 749 34 3130
Fragments arranged by size:
3130 749 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 330 1845 2901 3947 330
Size: 1515 1056 1046 330
Fragments arranged by size:
1515 1056 1046 330
BclI T'GATC_A
Cuts at: 1467 1467
Size: 3947
BfrBI ATG'CAT
Cuts at: 738 738
Size: 3947
BglI GCCn_nnn'nGGC
Cuts at: 422 476 422
Size: 54 3893
BglII A'GATC_T
Cuts at: 1462 3499 1462
Size: 2037 1910
BlpI GC'TnA_GC
Cuts at: 1059 1059
Size: 3947
Bme1580I G_kGCm'C
Cuts at: 701 731 1572 1665 2789 701
Size: 30 841 93 1124 1859
Fragments arranged by size:
1859 1124 841 93 30
BmrI ACTGGGnnnn_n'
Cuts at: 1159 1240 1575 3719 1159
Size: 81 335 2144 1387
Fragments arranged by size:
2144 1387 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 3947
Bpu10I CC'TnA_GC
Cuts at: 118 324 118
Size: 206 3741
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1123 2002 2716 3014 1123
Size: 879 714 298 2056
Fragments arranged by size:
2056 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3651 3651
Size: 3947
BsaAI yAC'GTr
Cuts at: 726 1300 726
Size: 574 3373
BsaBI GATnn'nnATC
Cuts at: 6 1461 3498 6
Size: 1455 2037 455
Fragments arranged by size:
2037 1455 455
BsaHI Gr'CG_yC
Cuts at: 515 1378 1626 2328 3824 515
Size: 863 248 702 1496 638
Fragments arranged by size:
1496 863 702 638 248
BsaWI w'CCGG_w
Cuts at: 1136 1657 2335 2746 2893 3167 1136
Size: 521 678 411 147 274 1916
Fragments arranged by size:
1916 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 775 1372 2130 2795 3203 775
Size: 597 758 665 408 1519
Fragments arranged by size:
1519 758 665 597 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 553
Size: 3947
BsiEI CG_ry'CG
Cuts at: 104 342 647 1489 1535 2765 104
Size: 238 305 842 46 1230 1286
Fragments arranged by size:
1286 1230 842 305 238 46
BsiHKAI G_wGCw'C
Cuts at: 245 701 731 961 1739 1929 2789 245
Size: 456 30 230 778 190 860 1403
Fragments arranged by size:
1403 860 778 456 230 190 30
BsiWI C'GTAC_G
Cuts at: 722 722
Size: 3947
BsmBI CGTCTCn'nnnn_
Cuts at: 712 712
Size: 3947
Bsp1286I G_dGCh'C
Cuts at: 245 701 731 961 1572 1665 1739 1929 1991
Size: 456 30 230 611 93 74 190 62
Cuts at: 1991 2789 245
Size: 798 1403
Fragments arranged by size:
1403 798 611 456 230 190 93 74
62 30
BspHI T'CATG_A
Cuts at: 2379 2379
Size: 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 997 1211 1513 1894 997
Size: 214 302 381 3050
Fragments arranged by size:
3050 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2239 2293 3777 3942 50
Size: 2189 54 1484 165 55
Fragments arranged by size:
2189 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1859 1859
Size: 3947
BsrFI r'CCGG_y
Cuts at: 646 1945 2126 646
Size: 1299 181 2467
Fragments arranged by size:
2467 1299 181
BssSI C'ACGA_G
Cuts at: 2218 2926 2218
Size: 708 3239
BstAPI GCAn_nnn'nTGC
Cuts at: 735 1324 735
Size: 589 3358
BstBI TT'CG_AA
Cuts at: 602 2308 602
Size: 1706 2241
BstEII G'GTnAC_C
Cuts at: 159 159
Size: 3947
BstYI r'GATC_y
Cuts at: 1 227 306 972 1439 1462 1797 2043 2349
Size: 226 79 666 467 23 335 246 306
Cuts at: 2349 2361 2447 3499 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 666 467 449 335 306 246 226
86 79 23 12
Bsu36I CC'TnA_GG
Cuts at: 473 473
Size: 3947
BtgI C'CryG_G
Cuts at: 164 164
Size: 3947
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1897 2078 2241 1897
Size: 181 163 3603
Fragments arranged by size:
3603 181 163
BtsI GCAGTG_nn'
Cuts at: 118 1190 118
Size: 1072 2875
DraI TTT'AAA
Cuts at: 946 946
Size: 3947
DrdI GACnn_nn'nnGTC
Cuts at: 1653 2997 1653
Size: 1344 2603
EaeI y'GGCC_r
Cuts at: 263 339 648 915 1532 1706 2097 2124 3469
Size: 76 309 267 617 174 391 27 1345
Cuts at: 3469 263
Size: 741
Fragments arranged by size:
1345 741 617 391 309 267 174 76
27
EagI C'GGCC_G
Cuts at: 339 1532 339
Size: 1193 2754
EarI CTCTTCn'nnn_
Cuts at: 336 604 856 1970 2180 336
Size: 268 252 1114 210 2103
Fragments arranged by size:
2103 1114 268 252 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2155 2897 3043 3757 2155
Size: 742 146 714 2345
Fragments arranged by size:
2345 742 714 146
EcoICRI GAG'CTC
Cuts at: 243 959 243
Size: 716 3231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 669 894 1772 2204 2557 3219 412
Size: 257 225 878 432 353 662 1140
Fragments arranged by size:
1140 878 662 432 353 257 225
EcoNI CCTnn'n_nnAGG
Cuts at: 925 925
Size: 3947
EcoO109I rG'GnC_Cy
Cuts at: 184 297 408 451 1086 184
Size: 113 111 43 635 3045
Fragments arranged by size:
3045 635 113 111 43
EcoRI G'AATT_C
Cuts at: 951 951
Size: 3947
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 552 1966 552
Size: 1414 2533
FspI TGC'GCA
Cuts at: 1728 1728
Size: 3947
HaeII r_GCGC'y
Cuts at: 202 518 534 861 1381 1629 2859 3630 202
Size: 316 16 327 520 248 1230 771 519
Fragments arranged by size:
1230 771 520 519 327 316 248 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 143 175 1789 1819 1821 1851 143
Size: 32 1614 30 2 30 2239
Fragments arranged by size:
2239 1614 32 30 30 2
HincII GTy'rAC
Cuts at: 986 986
Size: 3947
KasI G'GCGC_C
Cuts at: 514 1377 1625 514
Size: 863 248 2836
Fragments arranged by size:
2836 863 248
KpnI G_GTAC'C
Cuts at: 967 967
Size: 3947
MluI A'CGCG_T
Cuts at: 3380 3380
Size: 3947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2706 2890 2706
Size: 184 3763
MscI TGG'CCA
Cuts at: 265 1708 265
Size: 1443 2504
MslI CAynn'nnrTG
Cuts at: 166 1936 2063 166
Size: 1770 127 2050
Fragments arranged by size:
2050 1770 127
MspA1I CmG'CkG
Cuts at: 269 575 704 914 1058 1372 1732 2516 2761
Size: 306 129 210 144 314 360 784 245
Cuts at: 2761 269
Size: 1455
Fragments arranged by size:
1455 784 360 314 306 245 210 144
129
NaeI GCC'GGC
Cuts at: 2128 2128
Size: 3947
NarI GG'CG_CC
Cuts at: 515 1378 1626 515
Size: 863 248 2836
Fragments arranged by size:
2836 863 248
NcoI C'CATG_G
Cuts at: 164 164
Size: 3947
NgoMIV G'CCGG_C
Cuts at: 2126 2126
Size: 3947
NsiI A_TGCA'T
Cuts at: 740 740
Size: 3947
NspI r_CATG'y
Cuts at: 1000 1500 3103 1000
Size: 500 1603 1844
Fragments arranged by size:
1844 1603 500
PasI CC'CwG_GG
Cuts at: 180 180
Size: 3947
PciI A'CATG_T
Cuts at: 3099 3099
Size: 3947
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 3947
PmeI GTTT'AAAC
Cuts at: 946 946
Size: 3947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2393 2425 2393
Size: 32 3915
PpuMI rG'GwC_Cy
Cuts at: 184 297 408 451 184
Size: 113 111 43 3680
Fragments arranged by size:
3680 113 111 43
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 862 894 862
Size: 32 3915
PstI C_TGCA'G
Cuts at: 994 1679 994
Size: 685 3262
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3947
PvuII CAG'CTG
Cuts at: 704 1372 1732 704
Size: 668 360 2919
Fragments arranged by size:
2919 668 360
RsrII CG'GwC_CG
Cuts at: 2142 2142
Size: 3947
SacI G_AGCT'C
Cuts at: 245 961 245
Size: 716 3231
SalI G'TCGA_C
Cuts at: 984 984
Size: 3947
SanDI GG'GwC_CC
Cuts at: 184 408 451 184
Size: 224 43 3680
Fragments arranged by size:
3680 224 43
SapI GCTCTTCn'nnn_
Cuts at: 604 856 1970 2180 604
Size: 252 1114 210 2371
Fragments arranged by size:
2371 1114 252 210
SbfI CC_TGCA'GG
Cuts at: 994 994
Size: 3947
ScaI AGT'ACT
Cuts at: 3464 3464
Size: 3947
SfcI C'TryA_G
Cuts at: 33 879 990 1675 2643 2834 33
Size: 846 111 685 968 191 1146
Fragments arranged by size:
1146 968 846 685 191 111
SfiI GGCCn_nnn'nGGCC
Cuts at: 422 422
Size: 3947
SfoI GGC'GCC
Cuts at: 516 1379 1627 516
Size: 863 248 2836
Fragments arranged by size:
2836 863 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3947
SmaI CCC'GGG
Cuts at: 969 969
Size: 3947
SmlI C'TyrA_G
Cuts at: 1102 2017 2731 2993 1102
Size: 915 714 262 2056
Fragments arranged by size:
2056 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3947
SphI G_CATG'C
Cuts at: 1000 1500 1000
Size: 500 3447
StyI C'CwwG_G
Cuts at: 164 456 1081 1435 164
Size: 292 625 354 2676
Fragments arranged by size:
2676 625 354 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 617 831 1530 1663 2122 2331 617
Size: 214 699 133 459 209 2233
Fragments arranged by size:
2233 699 459 214 209 133
TatI w'GTAC_w
Cuts at: 3462 3462
Size: 3947
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3464 3496 3464
Size: 32 3915
Tth111I GACn'n_nGTC
Cuts at: 835 1744 835
Size: 909 3038
XbaI T'CTAG_A
Cuts at: 978 978
Size: 3947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 463 1442 463
Size: 979 2968
XmaI C'CCGG_G
Cuts at: 967 967
Size: 3947
XmnI GAAnn'nnTTC
Cuts at: 532 532
Size: 3947
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AloI ApaI AscI AvrII
BaeI BbsI BmgBI BmtI BpmI BsaXI BseRI BsmI
BspEI BsrGI BssHII BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PfoI PmlI PshAI PsiI PspOMI
PspXI SacII SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI