Restriction map for
pF1KB4594
(Circular) (Six-base) MAPSORT of: pF1KB4594.seq Check: 6996 from: 1 to: 5231
pF1KB4594 5231 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1350 2060 1350
Size: 710 4521
AccI GT'mk_AC
Cuts at: 2269 2269
Size: 5231
Acc65I G'GTAC_C
Cuts at: 2247 2247
Size: 5231
AclI AA'CG_TT
Cuts at: 5071 5071
Size: 5231
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2017 2062 3056 3488 4235 4503 2017
Size: 45 994 432 747 268 2745
Fragments arranged by size:
2745 994 747 432 268 45
AflIII A'CryG_T
Cuts at: 2583 3687 4664 2583
Size: 1104 977 3150
Fragments arranged by size:
3150 1104 977
AgeI A'CCGG_T
Cuts at: 1813 3619 1813
Size: 1806 3425
AhdI GACnn_n'nnGTC
Cuts at: 1383 1383
Size: 5231
AleI CACnn'nnGTG
Cuts at: 932 1958 1982 2170 932
Size: 1026 24 188 3993
Fragments arranged by size:
3993 1026 188 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1587 1587
Size: 5231
AlwNI CAG_nnn'CTG
Cuts at: 823 2040 2194 2536 4103 823
Size: 1217 154 342 1567 1951
Fragments arranged by size:
1951 1567 1217 342 154
ApaI G_GGCC'C
Cuts at: 1851 1851
Size: 5231
ApaLI G'TGCA_C
Cuts at: 1530 4001 1530
Size: 2471 2760
ApoI r'AATT_y
Cuts at: 1889 2235 4584 4840 1889
Size: 346 2349 256 2280
Fragments arranged by size:
2349 2280 346 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5231
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5231
AvaI C'yCGr_G
Cuts at: 2251 2251
Size: 5231
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 114 147 114
Size: 33 5198
BamHI G'GATC_C
Cuts at: 2256 2256
Size: 5231
BanI G'GyrC_C
Cuts at: 2014 2027 2152 2247 2661 2909 2944 2014
Size: 13 125 95 414 248 35 4301
Fragments arranged by size:
4301 414 248 125 95 35 13
BanII G_rGCy'C
Cuts at: 243 1061 1108 1207 1477 1851 2213 2245 3275
Size: 818 47 99 270 374 362 32 1030
Cuts at: 3275 243
Size: 2199
Fragments arranged by size:
2199 1030 818 374 362 270 99 47
32
BbeI G_GCGC'C
Cuts at: 2156 2665 2913 2156
Size: 509 248 4474
Fragments arranged by size:
4474 509 248
BbvCI CC'TCA_GC
Cuts at: 941 966 941
Size: 25 5206
BciVI GTATCCnnnnn_n'
Cuts at: 142 3129 3890 5231 142
Size: 2987 761 1341 142
Fragments arranged by size:
2987 1341 761 142
BclI T'GATC_A
Cuts at: 320 2751 320
Size: 2431 2800
BglI GCCn_nnn'nGGC
Cuts at: 118 1333 118
Size: 1215 4016
BglII A'GATC_T
Cuts at: 2746 4783 2746
Size: 2037 3194
BlpI GC'TnA_GC
Cuts at: 1301 2343 1301
Size: 1042 4189
Bme1580I G_kGCm'C
Cuts at: 795 1534 1851 1965 2856 2949 4005 795
Size: 739 317 114 891 93 1056 2021
Fragments arranged by size:
2021 1056 891 739 317 114 93
BmgBI CAC'GTC
Cuts at: 1535 1535
Size: 5231
BmrI ACTGGGnnnn_n'
Cuts at: 1614 1845 2443 2524 2859 5003 1614
Size: 231 598 81 335 2144 1842
Fragments arranged by size:
2144 1842 598 335 231 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1041 1518 1716 3598 1041
Size: 477 198 1882 2674
Fragments arranged by size:
2674 1882 477 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 1178 1366 1919 1159
Size: 19 188 553 4471
Fragments arranged by size:
4471 553 188 19
Bpu10I CC'TnA_GC
Cuts at: 941 966 1292 941
Size: 25 326 4880
Fragments arranged by size:
4880 326 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1690 2407 3286 3778 4076 1690
Size: 717 879 492 298 2845
Fragments arranged by size:
2845 879 717 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1014 1609 4935 1014
Size: 595 3326 1310
Fragments arranged by size:
3326 1310 595
BsaAI yAC'GTr
Cuts at: 2584 2584
Size: 5231
BsaBI GATnn'nnATC
Cuts at: 6 2745 4782 6
Size: 2739 2037 455
Fragments arranged by size:
2739 2037 455
BsaHI Gr'CG_yC
Cuts at: 2153 2662 2910 3612 5108 2153
Size: 509 248 702 1496 2276
Fragments arranged by size:
2276 1496 702 509 248
BsaWI w'CCGG_w
Cuts at: 182 755 1079 1813 2205 2420 2941 3619 3893
Size: 573 324 734 392 215 521 678 274
Cuts at: 3893 4040 4451 182
Size: 147 411 962
Fragments arranged by size:
962 734 678 573 521 411 392 324
274 215 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1461 1491 1461
Size: 30 5201
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 512 630 715 754 1017 1618 1708 512
Size: 118 85 39 263 601 90 4035
Fragments arranged by size:
4035 601 263 118 90 85 39
BseYI C'CCAG_C
Cuts at: 652 1305 2656 3414 3991 4487 652
Size: 653 1351 758 577 496 1396
Fragments arranged by size:
1396 1351 758 653 577 496
BsiEI CG_ry'CG
Cuts at: 104 841 2773 2819 4027 104
Size: 737 1932 46 1208 1308
Fragments arranged by size:
1932 1308 1208 737 46
BsiHKAI G_wGCw'C
Cuts at: 1256 1534 2213 2245 3023 3213 4005 1256
Size: 278 679 32 778 190 792 2482
Fragments arranged by size:
2482 792 778 679 278 190 32
BsmI GAATG_Cn'
Cuts at: 1167 1278 1167
Size: 111 5120
BsmBI CGTCTCn'nnnn_
Cuts at: 184 184
Size: 5231
Bsp1286I G_dGCh'C
Cuts at: 243 795 1061 1108 1207 1256 1477 1534 1851
Size: 552 266 47 99 49 221 57 317
Cuts at: 1851 1965 2213 2245 2856 2949 3023 3213 3275
Size: 114 248 32 611 93 74 190 62
Cuts at: 3275 4005 243
Size: 730 1469
Fragments arranged by size:
1469 730 611 552 317 266 248 221
190 114 99 93 74 62 57 49
47 32
BspEI T'CCGG_A
Cuts at: 182 755 2205 4451 182
Size: 573 1450 2246 962
Fragments arranged by size:
2246 1450 962 573
BspHI T'CATG_A
Cuts at: 4407 4407
Size: 5231
BspMI ACCTGCnnnn'nnnn_
Cuts at: 449 614 963 1350 1933 2060 2108 2207 2281
Size: 165 349 387 583 127 48 99 74
Cuts at: 2281 2495 2797 3178 449
Size: 214 302 381 2502
Fragments arranged by size:
2502 583 387 381 349 302 214 165
127 99 74 48
BsrBI CCG'CTC
Cuts at: 50 789 3523 3577 5061 5226 50
Size: 739 2734 54 1484 165 55
Fragments arranged by size:
2734 1484 739 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1674 3143 1674
Size: 1469 3762
BsrFI r'CCGG_y
Cuts at: 117 836 1439 1813 1859 3229 3410 3619 117
Size: 719 603 374 46 1370 181 209 1729
Fragments arranged by size:
1729 1370 719 603 374 209 181 46
BsrGI T'GTAC_A
Cuts at: 377 659 698 1941 377
Size: 282 39 1243 3667
Fragments arranged by size:
3667 1243 282 39
BssSI C'ACGA_G
Cuts at: 524 1322 3502 3860 524
Size: 798 2180 358 1895
Fragments arranged by size:
2180 1895 798 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1538 1719 2608 1538
Size: 181 889 4161
Fragments arranged by size:
4161 889 181
BstBI TT'CG_AA
Cuts at: 3592 3592
Size: 5231
BstXI CCAn_nnnn'nTGG
Cuts at: 1183 1585 2149 1183
Size: 402 564 4265
Fragments arranged by size:
4265 564 402
BstYI r'GATC_y
Cuts at: 1 330 2256 2723 2746 3081 3327 4339 4425
Size: 329 1926 467 23 335 246 1012 86
Cuts at: 4425 4437 4783 1
Size: 12 346 449
Fragments arranged by size:
1926 1012 467 449 346 335 329 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 347 1601 347
Size: 1254 3977
BtgI C'CryG_G
Cuts at: 107 428 1358 107
Size: 321 930 3980
Fragments arranged by size:
3980 930 321
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3181 3362 3525 3181
Size: 181 163 4887
Fragments arranged by size:
4887 181 163
BtsI GCAGTG_nn'
Cuts at: 273 2474 273
Size: 2201 3030
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1824 1859 1824
Size: 35 5196
DraI TTT'AAA
Cuts at: 2230 2230
Size: 5231
DraIII CAC_nnn'GTG
Cuts at: 919 919
Size: 5231
DrdI GACnn_nn'nnGTC
Cuts at: 2937 3795 2937
Size: 858 4373
EaeI y'GGCC_r
Cuts at: 425 767 838 1861 2816 2990 3381 3408 4753
Size: 342 71 1023 955 174 391 27 1345
Cuts at: 4753 425
Size: 903
Fragments arranged by size:
1345 1023 955 903 391 342 174 71
27
EagI C'GGCC_G
Cuts at: 838 2816 838
Size: 1978 3253
EarI CTCTTCn'nnn_
Cuts at: 524 733 902 1010 2038 3254 3464 524
Size: 209 169 108 1028 1216 210 2291
Fragments arranged by size:
2291 1216 1028 210 209 169 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3439 3749 3895 5041 3439
Size: 310 146 1146 3629
Fragments arranged by size:
3629 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2211 2243 2211
Size: 32 5199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 1178 1366 1919 2017 2062 3056 3488 4235
Size: 19 188 553 98 45 994 432 747
Cuts at: 4235 4503 1159
Size: 268 1887
Fragments arranged by size:
1887 994 747 553 432 268 188 98
45 19
EcoO109I rG'GnC_Cy
Cuts at: 945 1288 1655 1847 2370 945
Size: 343 367 192 523 3806
Fragments arranged by size:
3806 523 367 343 192
EcoRI G'AATT_C
Cuts at: 1889 2235 1889
Size: 346 4885
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3250 3250
Size: 5231
FspI TGC'GCA
Cuts at: 3012 3012
Size: 5231
HaeII r_GCGC'y
Cuts at: 623 2156 2665 2913 3935 4914 623
Size: 1533 509 248 1022 979 940
Fragments arranged by size:
1533 1022 979 940 509 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 344 376 1369 1401 1461 1493 1606 1638 3073
Size: 32 993 32 60 32 113 32 1435
Cuts at: 3073 3103 3105 3135 344
Size: 30 2 30 2440
Fragments arranged by size:
2440 1435 993 113 60 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1458 2270 1458
Size: 812 4419
HpaI GTT'AAC
Cuts at: 1458 1458
Size: 5231
KasI G'GCGC_C
Cuts at: 2152 2661 2909 2152
Size: 509 248 4474
Fragments arranged by size:
4474 509 248
KpnI G_GTAC'C
Cuts at: 2251 2251
Size: 5231
MluI A'CGCG_T
Cuts at: 4664 4664
Size: 5231
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3902 4086 3902
Size: 184 5047
MscI TGG'CCA
Cuts at: 427 769 2992 427
Size: 342 2223 2666
Fragments arranged by size:
2666 2223 342
MslI CAynn'nnrTG
Cuts at: 152 217 418 433 798 932 1814 1958 1982
Size: 65 201 15 365 134 882 144 24
Cuts at: 1982 2170 3220 3347 152
Size: 188 1050 127 2036
Fragments arranged by size:
2036 1050 882 365 201 188 144 134
127 65 24 15
MspA1I CmG'CkG
Cuts at: 128 1409 1585 1757 2124 2342 2656 3016 4029
Size: 1281 176 172 367 218 314 360 1013
Cuts at: 4029 4274 128
Size: 245 1085
Fragments arranged by size:
1281 1085 1013 367 360 314 245 218
176 172
NaeI GCC'GGC
Cuts at: 119 838 1441 3412 119
Size: 719 603 1971 1938
Fragments arranged by size:
1971 1938 719 603
NarI GG'CG_CC
Cuts at: 2153 2662 2910 2153
Size: 509 248 4474
Fragments arranged by size:
4474 509 248
NcoI C'CATG_G
Cuts at: 107 1358 107
Size: 1251 3980
NgoMIV G'CCGG_C
Cuts at: 117 836 1439 3410 117
Size: 719 603 1971 1938
Fragments arranged by size:
1971 1938 719 603
NspI r_CATG'y
Cuts at: 457 1518 1564 2284 2784 3691 457
Size: 1061 46 720 500 907 1997
Fragments arranged by size:
1997 1061 907 720 500 46
PasI CC'CwG_GG
Cuts at: 625 625
Size: 5231
PciI A'CATG_T
Cuts at: 3687 3687
Size: 5231
PflMI CCAn_nnn'nTGG
Cuts at: 823 823
Size: 5231
PfoI T'CCnGG_A
Cuts at: 365 365
Size: 5231
PmeI GTTT'AAAC
Cuts at: 2230 2230
Size: 5231
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4402 4370
Size: 32 5199
PspOMI G'GGCC_C
Cuts at: 1847 1847
Size: 5231
PstI C_TGCA'G
Cuts at: 268 446 1153 1724 2278 2963 268
Size: 178 707 571 554 685 2536
Fragments arranged by size:
2536 707 685 571 554 178
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5231
PvuII CAG'CTG
Cuts at: 128 2124 2656 3016 128
Size: 1996 532 360 2343
Fragments arranged by size:
2343 1996 532 360
RsrII CG'GwC_CG
Cuts at: 3426 3426
Size: 5231
SacI G_AGCT'C
Cuts at: 2213 2245 2213
Size: 32 5199
SalI G'TCGA_C
Cuts at: 2268 2268
Size: 5231
SapI GCTCTTCn'nnn_
Cuts at: 2038 3254 3464 2038
Size: 1216 210 3805
Fragments arranged by size:
3805 1216 210
SbfI CC_TGCA'GG
Cuts at: 2278 2278
Size: 5231
ScaI AGT'ACT
Cuts at: 1630 4748 1630
Size: 3118 2113
SfcI C'TryA_G
Cuts at: 33 264 279 442 930 1149 1720 2274 2959
Size: 231 15 163 488 219 571 554 685
Cuts at: 2959 3952 4143 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 571 554 488 231 219
191 163 15
SfoI GGC'GCC
Cuts at: 2154 2663 2911 2154
Size: 509 248 4474
Fragments arranged by size:
4474 509 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5231
SmaI CCC'GGG
Cuts at: 2253 2253
Size: 5231
SmlI C'TyrA_G
Cuts at: 1705 2386 3301 3793 4055 1705
Size: 681 915 492 262 2881
Fragments arranged by size:
2881 915 681 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5231
SphI G_CATG'C
Cuts at: 1564 2284 2784 1564
Size: 720 500 4011
Fragments arranged by size:
4011 720 500
StuI AGG'CCT
Cuts at: 1699 1765 1699
Size: 66 5165
StyI C'CwwG_G
Cuts at: 107 782 890 1358 2168 2365 2719 107
Size: 675 108 468 810 197 354 2619
Fragments arranged by size:
2619 810 675 468 354 197 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 617 903 1371 2814 2947 3406 3615 617
Size: 286 468 1443 133 459 209 2233
Fragments arranged by size:
2233 1443 468 459 286 209 133
TatI w'GTAC_w
Cuts at: 377 659 698 830 1628 1941 4746 377
Size: 282 39 132 798 313 2805 862
Fragments arranged by size:
2805 862 798 313 282 132 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4748 4780 4748
Size: 32 5199
Tth111I GACn'n_nGTC
Cuts at: 1620 3028 1620
Size: 1408 3823
XbaI T'CTAG_A
Cuts at: 327 2262 327
Size: 1935 3296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2726 2726
Size: 5231
XmaI C'CCGG_G
Cuts at: 2251 2251
Size: 5231
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI AvrII BbsI BcgI
BfrBI BmtI BsgI BsiWI BssHII BstEII BstZ17I ClaI
EcoNI EcoRV FseI FspAI HindIII MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI