Restriction map for
pF1KB4593
(Circular) (Six-base) MAPSORT of: pF1KB4593.seq Check: 7133 from: 1 to: 5453
pF1KB4593 5453 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 223 2491 223
Size: 2268 3185
Acc65I G'GTAC_C
Cuts at: 2469 2469
Size: 5453
AclI AA'CG_TT
Cuts at: 574 5293 574
Size: 4719 734
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1205 1781 1863 2267 2420 3278 3710 4063 4725
Size: 576 82 404 153 858 432 353 662
Cuts at: 4725 1205
Size: 1933
Fragments arranged by size:
1933 858 662 576 432 404 353 153
82
AfeI AGC'GCT
Cuts at: 541 541
Size: 5453
AflIII A'CryG_T
Cuts at: 1704 2805 4605 4886 1704
Size: 1101 1800 281 2271
Fragments arranged by size:
2271 1800 1101 281
AleI CACnn'nnGTG
Cuts at: 1243 1243
Size: 5453
AlwNI CAG_nnn'CTG
Cuts at: 262 2002 2095 2758 4196 262
Size: 1740 93 663 1438 1519
Fragments arranged by size:
1740 1519 1438 663 93
ApaLI G'TGCA_C
Cuts at: 4291 4291
Size: 5453
ApoI r'AATT_y
Cuts at: 133 381 511 590 649 852 883 1013 1404
Size: 248 130 79 59 203 31 130 391
Cuts at: 1404 1584 1653 2457 4806 5062 133
Size: 180 69 804 2349 256 524
Fragments arranged by size:
2349 804 524 391 256 248 203 180
130 130 79 69 59 31
AseI AT'TA_AT
Cuts at: 20 20
Size: 5453
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5453
AvaI C'yCGr_G
Cuts at: 194 2473 194
Size: 2279 3174
BamHI G'GATC_C
Cuts at: 2478 2478
Size: 5453
BanI G'GyrC_C
Cuts at: 823 2469 2883 3131 3166 823
Size: 1646 414 248 35 3110
Fragments arranged by size:
3110 1646 414 248 35
BanII G_rGCy'C
Cuts at: 1026 2467 3497 1026
Size: 1441 1030 2982
Fragments arranged by size:
2982 1441 1030
BbeI G_GCGC'C
Cuts at: 2887 3135 2887
Size: 248 5205
BbsI GAAGACnn'nnnn_
Cuts at: 569 569
Size: 5453
BciVI GTATCCnnnnn_n'
Cuts at: 3351 4407 5453 3351
Size: 1056 1046 3351
Fragments arranged by size:
3351 1056 1046
BclI T'GATC_A
Cuts at: 318 488 2280 2973 318
Size: 170 1792 693 2798
Fragments arranged by size:
2798 1792 693 170
BglII A'GATC_T
Cuts at: 1557 2968 5005 1557
Size: 1411 2037 2005
Fragments arranged by size:
2037 2005 1411
BlpI GC'TnA_GC
Cuts at: 2565 2565
Size: 5453
Bme1580I G_kGCm'C
Cuts at: 3078 3171 4295 3078
Size: 93 1124 4236
Fragments arranged by size:
4236 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2665 2746 3081 5225 2665
Size: 81 335 2144 2893
Fragments arranged by size:
2893 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3820 3820
Size: 5453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 284
Size: 5453
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 2629 3508 4222 4520 410
Size: 2219 879 714 298 1343
Fragments arranged by size:
2219 1343 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5157 5157
Size: 5453
BsaAI yAC'GTr
Cuts at: 848 2806 848
Size: 1958 3495
BsaBI GATnn'nnATC
Cuts at: 6 2967 5004 6
Size: 2961 2037 455
Fragments arranged by size:
2961 2037 455
BsaHI Gr'CG_yC
Cuts at: 395 2884 3132 3834 5330 395
Size: 2489 248 702 1496 518
Fragments arranged by size:
2489 1496 702 518 248
BsaWI w'CCGG_w
Cuts at: 1176 2642 3163 3841 4252 4399 4673 1176
Size: 1466 521 678 411 147 274 1956
Fragments arranged by size:
1956 1466 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1197 1227 1197
Size: 30 5423
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1094 1097 2120 1094
Size: 3 1023 4427
Fragments arranged by size:
4427 1023 3
BseYI C'CCAG_C
Cuts at: 865 2878 3636 4301 4709 865
Size: 2013 758 665 408 1609
Fragments arranged by size:
2013 1609 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 824 824
Size: 5453
BsiEI CG_ry'CG
Cuts at: 104 2995 3041 4271 104
Size: 2891 46 1230 1286
Fragments arranged by size:
2891 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1026 2467 3245 3435 4295 1026
Size: 1441 778 190 860 2184
Fragments arranged by size:
2184 1441 860 778 190
BsmI GAATG_Cn'
Cuts at: 927 927
Size: 5453
Bsp1286I G_dGCh'C
Cuts at: 1026 2467 3078 3171 3245 3435 3497 4295 1026
Size: 1441 611 93 74 190 62 798 2184
Fragments arranged by size:
2184 1441 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1857 3885 1857
Size: 2028 3425
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2503 2717 3019 3400 2503
Size: 214 302 381 4556
Fragments arranged by size:
4556 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3745 3799 5283 5448 50
Size: 3695 54 1484 165 55
Fragments arranged by size:
3695 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 795 2054 3365 795
Size: 1259 1311 2883
Fragments arranged by size:
2883 1311 1259
BsrFI r'CCGG_y
Cuts at: 3451 3632 3451
Size: 181 5272
BssSI C'ACGA_G
Cuts at: 3724 4432 3724
Size: 708 4745
BstAPI GCAn_nnn'nTGC
Cuts at: 2830 2830
Size: 5453
BstBI TT'CG_AA
Cuts at: 3814 3814
Size: 5453
BstYI r'GATC_y
Cuts at: 1 122 1557 2427 2478 2945 2968 3303 3549
Size: 121 1435 870 51 467 23 335 246
Cuts at: 3549 3855 3867 3953 5005 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1435 1052 870 467 449 335 306 246
121 86 51 23 12
Bsu36I CC'TnA_GG
Cuts at: 127 127
Size: 5453
BtgI C'CryG_G
Cuts at: 107 107
Size: 5453
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3403 3584 3747 3403
Size: 181 163 5109
Fragments arranged by size:
5109 181 163
BtsI GCAGTG_nn'
Cuts at: 807 1608 2696 807
Size: 801 1088 3564
Fragments arranged by size:
3564 1088 801
ClaI AT'CG_AT
Cuts at: 2400 2400
Size: 5453
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1184 1219 2367 2402 1184
Size: 35 1148 35 4235
Fragments arranged by size:
4235 1148 35 35
DraI TTT'AAA
Cuts at: 588 2452 588
Size: 1864 3589
DrdI GACnn_nn'nnGTC
Cuts at: 3159 4503 3159
Size: 1344 4109
EaeI y'GGCC_r
Cuts at: 697 3038 3212 3603 3630 4975 697
Size: 2341 174 391 27 1345 1175
Fragments arranged by size:
2341 1345 1175 391 174 27
EagI C'GGCC_G
Cuts at: 3038 3038
Size: 5453
EarI CTCTTCn'nnn_
Cuts at: 154 1882 3476 3686 154
Size: 1728 1594 210 1921
Fragments arranged by size:
1921 1728 1594 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3661 4403 4549 5263 3661
Size: 742 146 714 3851
Fragments arranged by size:
3851 742 714 146
EcoICRI GAG'CTC
Cuts at: 1024 2465 1024
Size: 1441 4012
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 1205 1781 1863 2267 2420 3278 3710 4063
Size: 921 576 82 404 153 858 432 353
Cuts at: 4063 4725 284
Size: 662 1012
Fragments arranged by size:
1012 921 858 662 576 432 404 353
153 82
EcoO109I rG'GnC_Cy
Cuts at: 430 1124 1428 2592 430
Size: 694 304 1164 3291
Fragments arranged by size:
3291 1164 694 304
EcoRI G'AATT_C
Cuts at: 1584 1653 2457 1584
Size: 69 804 4580
Fragments arranged by size:
4580 804 69
EcoRV GAT'ATC
Cuts at: 1779 1779
Size: 5453
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3472 3472
Size: 5453
FspI TGC'GCA
Cuts at: 3234 3234
Size: 5453
HaeII r_GCGC'y
Cuts at: 543 2887 3135 4365 5136 543
Size: 2344 248 1230 771 860
Fragments arranged by size:
2344 1230 860 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 747 779 1197 1229 1516 1548 1912 1944 3295
Size: 32 418 32 287 32 364 32 1351
Cuts at: 3295 3325 3327 3357 747
Size: 30 2 30 2843
Fragments arranged by size:
2843 1351 418 364 287 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2492 2492
Size: 5453
HindIII A'AGCT_T
Cuts at: 747 747
Size: 5453
KasI G'GCGC_C
Cuts at: 2883 3131 2883
Size: 248 5205
KpnI G_GTAC'C
Cuts at: 2473 2473
Size: 5453
MfeI C'AATT_G
Cuts at: 1373 1373
Size: 5453
MluI A'CGCG_T
Cuts at: 4886 4886
Size: 5453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1255 2392 4212 4396 1255
Size: 1137 1820 184 2312
Fragments arranged by size:
2312 1820 1137 184
MscI TGG'CCA
Cuts at: 699 3214 699
Size: 2515 2938
MslI CAynn'nnrTG
Cuts at: 496 776 800 1243 1916 2090 3442 3569 496
Size: 280 24 443 673 174 1352 127 2380
Fragments arranged by size:
2380 1352 673 443 280 174 127 24
MspA1I CmG'CkG
Cuts at: 2290 2564 2878 3238 4022 4267 2290
Size: 274 314 360 784 245 3476
Fragments arranged by size:
3476 784 360 314 274 245
NaeI GCC'GGC
Cuts at: 3634 3634
Size: 5453
NarI GG'CG_CC
Cuts at: 2884 3132 2884
Size: 248 5205
NcoI C'CATG_G
Cuts at: 107 107
Size: 5453
NdeI CA'TA_TG
Cuts at: 414 1289 414
Size: 875 4578
NgoMIV G'CCGG_C
Cuts at: 3632 3632
Size: 5453
NspI r_CATG'y
Cuts at: 1489 1708 2506 3006 4609 1489
Size: 219 798 500 1603 2333
Fragments arranged by size:
2333 1603 798 500 219
PciI A'CATG_T
Cuts at: 1704 4605 1704
Size: 2901 2552
PmeI GTTT'AAAC
Cuts at: 2452 2452
Size: 5453
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 3899
Size: 32 5421
PpuMI rG'GwC_Cy
Cuts at: 430 1124 1428 430
Size: 694 304 4455
Fragments arranged by size:
4455 694 304
PsiI TTA'TAA
Cuts at: 388 388
Size: 5453
PstI C_TGCA'G
Cuts at: 936 1172 1603 2500 3185 936
Size: 236 431 897 685 3204
Fragments arranged by size:
3204 897 685 431 236
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5453
PvuII CAG'CTG
Cuts at: 2878 3238 2878
Size: 360 5093
RsrII CG'GwC_CG
Cuts at: 3648 3648
Size: 5453
SacI G_AGCT'C
Cuts at: 1026 2467 1026
Size: 1441 4012
SalI G'TCGA_C
Cuts at: 2490 2490
Size: 5453
SapI GCTCTTCn'nnn_
Cuts at: 3476 3686 3476
Size: 210 5243
SbfI CC_TGCA'GG
Cuts at: 2500 2500
Size: 5453
ScaI AGT'ACT
Cuts at: 2016 4970 2016
Size: 2954 2499
SfcI C'TryA_G
Cuts at: 33 932 1168 1332 1599 2496 3181 4149 4340
Size: 899 236 164 267 897 685 968 191
Cuts at: 4340 33
Size: 1146
Fragments arranged by size:
1146 968 899 897 685 267 236 191
164
SfoI GGC'GCC
Cuts at: 2885 3133 2885
Size: 248 5205
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5453
SmaI CCC'GGG
Cuts at: 196 2475 196
Size: 2279 3174
SmlI C'TyrA_G
Cuts at: 425 2608 3523 4237 4499 425
Size: 2183 915 714 262 1379
Fragments arranged by size:
2183 1379 915 714 262
SpeI A'CTAG_T
Cuts at: 67 2011 67
Size: 1944 3509
SphI G_CATG'C
Cuts at: 2506 3006 2506
Size: 500 4953
SspI AAT'ATT
Cuts at: 1977 1977
Size: 5453
StyI C'CwwG_G
Cuts at: 107 1120 1669 2587 2941 107
Size: 1013 549 918 354 2619
Fragments arranged by size:
2619 1013 918 549 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1212 3036 3169 3628 3837 1212
Size: 1824 133 459 209 2828
Fragments arranged by size:
2828 1824 459 209 133
TatI w'GTAC_w
Cuts at: 936 2014 4968 936
Size: 1078 2954 1421
Fragments arranged by size:
2954 1421 1078
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 519 551 4970 5002 519
Size: 32 4419 32 970
Fragments arranged by size:
4419 970 32 32
Tth111I GACn'n_nGTC
Cuts at: 3250 3250
Size: 5453
XbaI T'CTAG_A
Cuts at: 2484 2484
Size: 5453
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2438 2948 114
Size: 2324 510 2619
Fragments arranged by size:
2619 2324 510
XmaI C'CCGG_G
Cuts at: 194 2473 194
Size: 2279 3174
XmnI GAAnn'nnTTC
Cuts at: 779 962 779
Size: 183 5270
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstXI BstZ17I DraIII EcoNI FseI FspAI HpaI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI