Restriction map for
pF1KB4569
(Circular) (Six-base) MAPSORT of: pF1KB4569.seq Check: 1402 from: 1 to: 4757
pF1KB4569 4757 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 459 1504 1708 459
Size: 1045 204 3508
Fragments arranged by size:
3508 1045 204
AccI GT'mk_AC
Cuts at: 1460 1795 1460
Size: 335 4422
Acc65I G'GTAC_C
Cuts at: 1773 1773
Size: 4757
AclI AA'CG_TT
Cuts at: 4597 4597
Size: 4757
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 586 2582 3014 3761 4029 182
Size: 404 1996 432 747 268 910
Fragments arranged by size:
1996 910 747 432 404 268
AflIII A'CryG_T
Cuts at: 2109 3213 4190 2109
Size: 1104 977 2676
Fragments arranged by size:
2676 1104 977
AgeI A'CCGG_T
Cuts at: 3145 3145
Size: 4757
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1369 1369
Size: 4757
AlwNI CAG_nnn'CTG
Cuts at: 1595 2062 3629 1595
Size: 467 1567 2723
Fragments arranged by size:
2723 1567 467
ApaI G_GGCC'C
Cuts at: 1482 1482
Size: 4757
ApaLI G'TGCA_C
Cuts at: 3527 3527
Size: 4757
ApoI r'AATT_y
Cuts at: 151 349 1761 4110 4366 151
Size: 198 1412 2349 256 542
Fragments arranged by size:
2349 1412 542 256 198
AseI AT'TA_AT
Cuts at: 20 20
Size: 4757
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
AvaI C'yCGr_G
Cuts at: 1777 1777
Size: 4757
BamHI G'GATC_C
Cuts at: 632 1782 632
Size: 1150 3607
BanI G'GyrC_C
Cuts at: 429 1492 1773 2187 2435 2470 429
Size: 1063 281 414 248 35 2716
Fragments arranged by size:
2716 1063 414 281 248 35
BanII G_rGCy'C
Cuts at: 969 1482 1771 2801 969
Size: 513 289 1030 2925
Fragments arranged by size:
2925 1030 513 289
BbeI G_GCGC'C
Cuts at: 2191 2439 2191
Size: 248 4509
BbsI GAAGACnn'nnnn_
Cuts at: 1013 1315 1013
Size: 302 4455
BbvCI CC'TCA_GC
Cuts at: 418 418
Size: 4757
BciVI GTATCCnnnnn_n'
Cuts at: 2655 3416 4757 2655
Size: 761 1341 2655
Fragments arranged by size:
2655 1341 761
BclI T'GATC_A
Cuts at: 2277 2277
Size: 4757
BfrBI ATG'CAT
Cuts at: 1090 1090
Size: 4757
BglI GCCn_nnn'nGGC
Cuts at: 656 656
Size: 4757
BglII A'GATC_T
Cuts at: 2272 4309 2272
Size: 2037 2720
BlpI GC'TnA_GC
Cuts at: 1869 1869
Size: 4757
Bme1580I G_kGCm'C
Cuts at: 278 1482 1495 2382 2475 3531 278
Size: 1204 13 887 93 1056 1504
Fragments arranged by size:
1504 1204 1056 887 93 13
BmrI ACTGGGnnnn_n'
Cuts at: 675 1969 2050 2385 4529 675
Size: 1294 81 335 2144 903
Fragments arranged by size:
2144 1294 903 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3124 3124
Size: 4757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 981 1143 1442 981
Size: 162 299 4296
Fragments arranged by size:
4296 299 162
Bpu10I CC'TnA_GC
Cuts at: 130 418 130
Size: 288 4469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1933 2812 3304 3602 1933
Size: 879 492 298 3088
Fragments arranged by size:
3088 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1633 4461 1633
Size: 2828 1929
BsaAI yAC'GTr
Cuts at: 234 2110 234
Size: 1876 2881
BsaBI GATnn'nnATC
Cuts at: 6 412 2271 4308 6
Size: 406 1859 2037 455
Fragments arranged by size:
2037 1859 455 406
BsaHI Gr'CG_yC
Cuts at: 2188 2436 3138 4634 2188
Size: 248 702 1496 2311
Fragments arranged by size:
2311 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1946 2467 3145 3419 3566 3977 1946
Size: 521 678 274 147 411 2726
Fragments arranged by size:
2726 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 686 841 844 1231 127
Size: 559 155 3 387 3653
Fragments arranged by size:
3653 559 387 155 3
BseYI C'CCAG_C
Cuts at: 805 978 2182 2940 3517 4013 805
Size: 173 1204 758 577 496 1549
Fragments arranged by size:
1549 1204 758 577 496 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1736 1736
Size: 4757
BsiEI CG_ry'CG
Cuts at: 104 1701 2299 2345 3553 104
Size: 1597 598 46 1208 1308
Fragments arranged by size:
1597 1308 1208 598 46
BsiHKAI G_wGCw'C
Cuts at: 644 969 1385 1524 1771 2549 2739 3531 644
Size: 325 416 139 247 778 190 792 1870
Fragments arranged by size:
1870 792 778 416 325 247 190 139
BsmI GAATG_Cn'
Cuts at: 1090 1712 1090
Size: 622 4135
Bsp1286I G_dGCh'C
Cuts at: 278 644 969 1385 1482 1495 1524 1771 2382
Size: 366 325 416 97 13 29 247 611
Cuts at: 2382 2475 2549 2739 2801 3531 278
Size: 93 74 190 62 730 1504
Fragments arranged by size:
1504 730 611 416 366 325 247 190
97 93 74 62 29 13
BspEI T'CCGG_A
Cuts at: 3977 3977
Size: 4757
BspHI T'CATG_A
Cuts at: 3933 3933
Size: 4757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 459 1504 1708 1807 2021 2323 2704 459
Size: 1045 204 99 214 302 381 2512
Fragments arranged by size:
2512 1045 381 302 214 204 99
BsrBI CCG'CTC
Cuts at: 50 849 3049 3103 4587 4752 50
Size: 799 2200 54 1484 165 55
Fragments arranged by size:
2200 1484 799 165 55 54
BsrDI GCAATG_nn'
Cuts at: 441 475 1400 2669 441
Size: 34 925 1269 2529
Fragments arranged by size:
2529 1269 925 34
BsrFI r'CCGG_y
Cuts at: 141 2755 2936 3145 141
Size: 2614 181 209 1753
Fragments arranged by size:
2614 1753 209 181
BssSI C'ACGA_G
Cuts at: 3028 3386 3028
Size: 358 4399
BstAPI GCAn_nnn'nTGC
Cuts at: 2134 2134
Size: 4757
BstBI TT'CG_AA
Cuts at: 3118 3118
Size: 4757
BstXI CCAn_nnnn'nTGG
Cuts at: 978 978
Size: 4757
BstYI r'GATC_y
Cuts at: 1 632 1782 2249 2272 2607 2853 3865 3951
Size: 631 1150 467 23 335 246 1012 86
Cuts at: 3951 3963 4309 1
Size: 12 346 449
Fragments arranged by size:
1150 1012 631 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1454 1454
Size: 4757
BtgI C'CryG_G
Cuts at: 107 401 1313 1466 107
Size: 294 912 153 3398
Fragments arranged by size:
3398 912 294 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1524 2707 2888 3051 1524
Size: 1183 181 163 3230
Fragments arranged by size:
3230 1183 181 163
BtsI GCAGTG_nn'
Cuts at: 332 2000 332
Size: 1668 3089
DraI TTT'AAA
Cuts at: 1756 1756
Size: 4757
DrdI GACnn_nn'nnGTC
Cuts at: 2463 3321 2463
Size: 858 3899
EaeI y'GGCC_r
Cuts at: 1698 2342 2516 2907 2934 4279 1698
Size: 644 174 391 27 1345 2176
Fragments arranged by size:
2176 1345 644 391 174 27
EagI C'GGCC_G
Cuts at: 1698 2342 1698
Size: 644 4113
EarI CTCTTCn'nnn_
Cuts at: 1135 1402 2780 2990 1135
Size: 267 1378 210 2902
Fragments arranged by size:
2902 1378 267 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2965 3275 3421 4567 2965
Size: 310 146 1146 3155
Fragments arranged by size:
3155 1146 310 146
EcoICRI GAG'CTC
Cuts at: 967 1769 967
Size: 802 3955
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 586 981 1143 1442 2582 3014 3761 4029
Size: 404 395 162 299 1140 432 747 268
Cuts at: 4029 182
Size: 910
Fragments arranged by size:
1140 910 747 432 404 395 299 268
162
EcoNI CCTnn'n_nnAGG
Cuts at: 942 1213 942
Size: 271 4486
EcoO109I rG'GnC_Cy
Cuts at: 648 1026 1479 1896 648
Size: 378 453 417 3509
Fragments arranged by size:
3509 453 417 378
EcoRI G'AATT_C
Cuts at: 1761 1761
Size: 4757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2776 2776
Size: 4757
FspI TGC'GCA
Cuts at: 2538 2538
Size: 4757
HaeII r_GCGC'y
Cuts at: 2191 2439 3461 4440 2191
Size: 248 1022 979 2508
Fragments arranged by size:
2508 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 892 924 2599 2629 2631 2661 892
Size: 32 1675 30 2 30 2988
Fragments arranged by size:
2988 1675 32 30 30 2
HincII GTy'rAC
Cuts at: 1796 1796
Size: 4757
KasI G'GCGC_C
Cuts at: 2187 2435 2187
Size: 248 4509
KpnI G_GTAC'C
Cuts at: 1777 1777
Size: 4757
MluI A'CGCG_T
Cuts at: 4190 4190
Size: 4757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1144 1350 3428 3612 1144
Size: 206 2078 184 2289
Fragments arranged by size:
2289 2078 206 184
MscI TGG'CCA
Cuts at: 2518 2518
Size: 4757
MslI CAynn'nnrTG
Cuts at: 406 2746 2873 406
Size: 2340 127 2290
Fragments arranged by size:
2340 2290 127
MspA1I CmG'CkG
Cuts at: 138 271 438 841 978 1502 1868 2182 2542
Size: 133 167 403 137 524 366 314 360
Cuts at: 2542 3555 3800 138
Size: 1013 245 1095
Fragments arranged by size:
1095 1013 524 403 366 360 314 245
167 137 133
NaeI GCC'GGC
Cuts at: 2938 2938
Size: 4757
NarI GG'CG_CC
Cuts at: 2188 2436 2188
Size: 248 4509
NcoI C'CATG_G
Cuts at: 107 401 1313 107
Size: 294 912 3551
Fragments arranged by size:
3551 912 294
NdeI CA'TA_TG
Cuts at: 1440 1440
Size: 4757
NgoMIV G'CCGG_C
Cuts at: 2936 2936
Size: 4757
NsiI A_TGCA'T
Cuts at: 1092 1092
Size: 4757
NspI r_CATG'y
Cuts at: 1810 2310 3217 1810
Size: 500 907 3350
Fragments arranged by size:
3350 907 500
PciI A'CATG_T
Cuts at: 3213 3213
Size: 4757
PflMI CCAn_nnn'nTGG
Cuts at: 524 1692 524
Size: 1168 3589
PmeI GTTT'AAAC
Cuts at: 1756 1756
Size: 4757
PmlI CAC'GTG
Cuts at: 234 234
Size: 4757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1726 1758 3896 3928 1726
Size: 32 2138 32 2555
Fragments arranged by size:
2555 2138 32 32
PpuMI rG'GwC_Cy
Cuts at: 1026 1026
Size: 4757
PspOMI G'GGCC_C
Cuts at: 1478 1478
Size: 4757
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 688 720 688
Size: 32 4725
PstI C_TGCA'G
Cuts at: 1348 1501 1661 1804 2489 1348
Size: 153 160 143 685 3616
Fragments arranged by size:
3616 685 160 153 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4757
PvuII CAG'CTG
Cuts at: 138 271 438 841 2182 2542 138
Size: 133 167 403 1341 360 2353
Fragments arranged by size:
2353 1341 403 360 167 133
RsrII CG'GwC_CG
Cuts at: 2952 2952
Size: 4757
SacI G_AGCT'C
Cuts at: 969 1771 969
Size: 802 3955
SalI G'TCGA_C
Cuts at: 1794 1794
Size: 4757
SapI GCTCTTCn'nnn_
Cuts at: 2780 2990 2780
Size: 210 4547
SbfI CC_TGCA'GG
Cuts at: 1804 1804
Size: 4757
ScaI AGT'ACT
Cuts at: 4274 4274
Size: 4757
SexAI A'CCwGG_T
Cuts at: 1726 1726
Size: 4757
SfcI C'TryA_G
Cuts at: 33 1175 1344 1497 1657 1800 2485 3478 3669
Size: 1142 169 153 160 143 685 993 191
Cuts at: 3669 33
Size: 1121
Fragments arranged by size:
1142 1121 993 685 191 169 160 153
143
SfoI GGC'GCC
Cuts at: 2189 2437 2189
Size: 248 4509
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
SmaI CCC'GGG
Cuts at: 1779 1779
Size: 4757
SmlI C'TyrA_G
Cuts at: 1912 2827 3319 3581 1912
Size: 915 492 262 3088
Fragments arranged by size:
3088 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4757
SphI G_CATG'C
Cuts at: 1810 2310 1810
Size: 500 4257
StyI C'CwwG_G
Cuts at: 107 278 401 1313 1891 2245 107
Size: 171 123 912 578 354 2619
Fragments arranged by size:
2619 912 578 354 171 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1025 1507 2340 2473 2932 3141 1025
Size: 482 833 133 459 209 2641
Fragments arranged by size:
2641 833 482 459 209 133
TatI w'GTAC_w
Cuts at: 590 4272 590
Size: 3682 1075
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1222 1254 1364 1396 4274 4306 1222
Size: 32 110 32 2878 32 1673
Fragments arranged by size:
2878 1673 110 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2554 2554
Size: 4757
XbaI T'CTAG_A
Cuts at: 1788 1788
Size: 4757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2252 2252
Size: 4757
XmaI C'CCGG_G
Cuts at: 1777 1777
Size: 4757
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PmeI PmlI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI PacI
PasI PfoI PshAI PsiI PspXI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI