Restriction map for
pF1KB4568
(Circular) (Six-base) MAPSORT of: pF1KB4568.seq Check: 2531 from: 1 to: 4223
pF1KB4568 4223 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 1261 1261
Size: 4223
Acc65I G'GTAC_C
Cuts at: 1239 1239
Size: 4223
AclI AA'CG_TT
Cuts at: 525 4063 525
Size: 3538 685
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2048 2480 3227 3495 2048
Size: 432 747 268 2776
Fragments arranged by size:
2776 747 432 268
AflIII A'CryG_T
Cuts at: 1575 2679 3656 1575
Size: 1104 977 2142
Fragments arranged by size:
2142 1104 977
AgeI A'CCGG_T
Cuts at: 2611 2611
Size: 4223
AleI CACnn'nnGTG
Cuts at: 625 625
Size: 4223
AlwNI CAG_nnn'CTG
Cuts at: 1528 3095 1528
Size: 1567 2656
ApaLI G'TGCA_C
Cuts at: 2993 2993
Size: 4223
ApoI r'AATT_y
Cuts at: 886 1227 3576 3832 886
Size: 341 2349 256 1277
Fragments arranged by size:
2349 1277 341 256
AseI AT'TA_AT
Cuts at: 20 404 20
Size: 384 3839
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
AvaI C'yCGr_G
Cuts at: 1243 1243
Size: 4223
BamHI G'GATC_C
Cuts at: 374 1248 374
Size: 874 3349
BanI G'GyrC_C
Cuts at: 293 569 583 1239 1653 1901 1936 293
Size: 276 14 656 414 248 35 2580
Fragments arranged by size:
2580 656 414 276 248 35 14
BanII G_rGCy'C
Cuts at: 265 451 1237 2267 265
Size: 186 786 1030 2221
Fragments arranged by size:
2221 1030 786 186
BbeI G_GCGC'C
Cuts at: 297 573 1657 1905 297
Size: 276 1084 248 2615
Fragments arranged by size:
2615 1084 276 248
BbsI GAAGACnn'nnnn_
Cuts at: 345 345
Size: 4223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4189
BciVI GTATCCnnnnn_n'
Cuts at: 2121 2882 4223 2121
Size: 761 1341 2121
Fragments arranged by size:
2121 1341 761
BclI T'GATC_A
Cuts at: 1743 1743
Size: 4223
BglI GCCn_nnn'nGGC
Cuts at: 139 314 334 139
Size: 175 20 4028
Fragments arranged by size:
4028 175 20
BglII A'GATC_T
Cuts at: 397 1738 3775 397
Size: 1341 2037 845
Fragments arranged by size:
2037 1341 845
BlpI GC'TnA_GC
Cuts at: 1121 1335 1121
Size: 214 4009
Bme1580I G_kGCm'C
Cuts at: 1848 1941 2997 1848
Size: 93 1056 3074
Fragments arranged by size:
3074 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 759 1435 1516 1851 3995 759
Size: 676 81 335 2144 987
Fragments arranged by size:
2144 987 676 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2590 2590
Size: 4223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 473 724 866 1072 455
Size: 18 251 142 206 3606
Fragments arranged by size:
3606 251 206 142 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1399 2278 2770 3068 1399
Size: 879 492 298 2554
Fragments arranged by size:
2554 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3927 3927
Size: 4223
BsaAI yAC'GTr
Cuts at: 1576 1576
Size: 4223
BsaBI GATnn'nnATC
Cuts at: 6 1737 3774 6
Size: 1731 2037 455
Fragments arranged by size:
2037 1731 455
BsaHI Gr'CG_yC
Cuts at: 294 350 570 1654 1902 2604 4100 294
Size: 56 220 1084 248 702 1496 417
Fragments arranged by size:
1496 1084 702 417 248 220 56
BsaWI w'CCGG_w
Cuts at: 1412 1933 2611 2885 3032 3443 1412
Size: 521 678 274 147 411 2192
Fragments arranged by size:
2192 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 403 373
Size: 30 4193
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 382 397 400 268
Size: 114 15 3 4091
Fragments arranged by size:
4091 114 15 3
BseYI C'CCAG_C
Cuts at: 1648 2406 2983 3479 1648
Size: 758 577 496 2392
Fragments arranged by size:
2392 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 1045 286
Size: 759 3464
BsiEI CG_ry'CG
Cuts at: 104 246 285 309 1765 1811 3019 104
Size: 142 39 24 1456 46 1208 1308
Fragments arranged by size:
1456 1308 1208 142 46 39 24
BsiHKAI G_wGCw'C
Cuts at: 265 451 1237 2015 2205 2997 265
Size: 186 786 778 190 792 1491
Fragments arranged by size:
1491 792 786 778 190 186
Bsp1286I G_dGCh'C
Cuts at: 265 451 1237 1848 1941 2015 2205 2267 2997
Size: 186 786 611 93 74 190 62 730
Cuts at: 2997 265
Size: 1491
Fragments arranged by size:
1491 786 730 611 190 186 93 74
62
BspEI T'CCGG_A
Cuts at: 3443 3443
Size: 4223
BspHI T'CATG_A
Cuts at: 3399 3399
Size: 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1273 1487 1789 2170 1273
Size: 214 302 381 3326
Fragments arranged by size:
3326 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2515 2569 4053 4218 50
Size: 2465 54 1484 165 55
Fragments arranged by size:
2465 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2135 2135
Size: 4223
BsrFI r'CCGG_y
Cuts at: 296 346 2221 2402 2611 296
Size: 50 1875 181 209 1908
Fragments arranged by size:
1908 1875 209 181 50
BssHII G'CGCG_C
Cuts at: 216 218 216
Size: 2 4221
BssSI C'ACGA_G
Cuts at: 2494 2852 2494
Size: 358 3865
BstAPI GCAn_nnn'nTGC
Cuts at: 1600 1600
Size: 4223
BstBI TT'CG_AA
Cuts at: 2584 2584
Size: 4223
BstEII G'GTnAC_C
Cuts at: 753 753
Size: 4223
BstXI CCAn_nnnn'nTGG
Cuts at: 1089 1089
Size: 4223
BstYI r'GATC_y
Cuts at: 1 374 397 1248 1715 1738 2073 2319 3331
Size: 373 23 851 467 23 335 246 1012
Cuts at: 3331 3417 3429 3775 1
Size: 86 12 346 449
Fragments arranged by size:
1012 851 467 449 373 346 335 246
86 23 23 12
BtgI C'CryG_G
Cuts at: 115 115
Size: 4223
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2173 2354 2517 2173
Size: 181 163 3879
Fragments arranged by size:
3879 181 163
BtsI GCAGTG_nn'
Cuts at: 1466 1466
Size: 4223
DraI TTT'AAA
Cuts at: 1222 1222
Size: 4223
DrdI GACnn_nn'nnGTC
Cuts at: 1929 2787 1929
Size: 858 3365
EaeI y'GGCC_r
Cuts at: 128 176 243 282 306 315 1067 1808 1982
Size: 48 67 39 24 9 752 741 174
Cuts at: 1982 2373 2400 3745 128
Size: 391 27 1345 606
Fragments arranged by size:
1345 752 741 606 391 174 67 48
39 27 24 9
EagI C'GGCC_G
Cuts at: 243 282 306 1808 243
Size: 39 24 1502 2658
Fragments arranged by size:
2658 1502 39 24
EarI CTCTTCn'nnn_
Cuts at: 2246 2456 2246
Size: 210 4013
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2431 2741 2887 4033 2431
Size: 310 146 1146 2621
Fragments arranged by size:
2621 1146 310 146
EcoICRI GAG'CTC
Cuts at: 263 449 1235 263
Size: 186 786 3251
Fragments arranged by size:
3251 786 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 473 724 866 1072 2048 2480 3227 3495
Size: 18 251 142 206 976 432 747 268
Cuts at: 3495 455
Size: 1183
Fragments arranged by size:
1183 976 747 432 268 251 206 142
18
EcoO109I rG'GnC_Cy
Cuts at: 159 1362 159
Size: 1203 3020
EcoRI G'AATT_C
Cuts at: 886 1227 886
Size: 341 3882
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 570 2242 570
Size: 1672 2551
FspI TGC'GCA
Cuts at: 2004 2004
Size: 4223
HaeII r_GCGC'y
Cuts at: 172 297 573 1657 1905 2927 3906 172
Size: 125 276 1084 248 1022 979 489
Fragments arranged by size:
1084 1022 979 489 276 248 125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1099 1131 2065 2095 2097 2127 1099
Size: 32 934 30 2 30 3195
Fragments arranged by size:
3195 934 32 30 30 2
HincII GTy'rAC
Cuts at: 1262 1262
Size: 4223
KasI G'GCGC_C
Cuts at: 293 569 1653 1901 293
Size: 276 1084 248 2615
Fragments arranged by size:
2615 1084 276 248
KpnI G_GTAC'C
Cuts at: 1243 1243
Size: 4223
MluI A'CGCG_T
Cuts at: 3656 3656
Size: 4223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 401 1000 2894 3078 401
Size: 599 1894 184 1546
Fragments arranged by size:
1894 1546 599 184
MscI TGG'CCA
Cuts at: 178 317 1069 1984 178
Size: 139 752 915 2417
Fragments arranged by size:
2417 915 752 139
MslI CAynn'nnrTG
Cuts at: 625 835 866 2212 2339 625
Size: 210 31 1346 127 2509
Fragments arranged by size:
2509 1346 210 127 31
MspA1I CmG'CkG
Cuts at: 117 657 1190 1334 1648 2008 3021 3266 117
Size: 540 533 144 314 360 1013 245 1074
Fragments arranged by size:
1074 1013 540 533 360 314 245 144
NaeI GCC'GGC
Cuts at: 298 348 2404 298
Size: 50 2056 2117
Fragments arranged by size:
2117 2056 50
NarI GG'CG_CC
Cuts at: 294 570 1654 1902 294
Size: 276 1084 248 2615
Fragments arranged by size:
2615 1084 276 248
NdeI CA'TA_TG
Cuts at: 906 906
Size: 4223
NgoMIV G'CCGG_C
Cuts at: 296 346 2402 296
Size: 50 2056 2117
Fragments arranged by size:
2117 2056 50
NspI r_CATG'y
Cuts at: 1041 1276 1776 2683 1041
Size: 235 500 907 2581
Fragments arranged by size:
2581 907 500 235
PciI A'CATG_T
Cuts at: 2679 2679
Size: 4223
PfoI T'CCnGG_A
Cuts at: 377 1049 377
Size: 672 3551
PmeI GTTT'AAAC
Cuts at: 1222 1222
Size: 4223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 4191
PstI C_TGCA'G
Cuts at: 1270 1955 1270
Size: 685 3538
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4223
PvuII CAG'CTG
Cuts at: 657 1190 1648 2008 657
Size: 533 458 360 2872
Fragments arranged by size:
2872 533 458 360
RsrII CG'GwC_CG
Cuts at: 2418 2418
Size: 4223
SacI G_AGCT'C
Cuts at: 265 451 1237 265
Size: 186 786 3251
Fragments arranged by size:
3251 786 186
SacII CC_GC'GG
Cuts at: 118 118
Size: 4223
SalI G'TCGA_C
Cuts at: 1260 1260
Size: 4223
SapI GCTCTTCn'nnn_
Cuts at: 2246 2456 2246
Size: 210 4013
SbfI CC_TGCA'GG
Cuts at: 1270 1270
Size: 4223
ScaI AGT'ACT
Cuts at: 3740 3740
Size: 4223
SfcI C'TryA_G
Cuts at: 33 1266 1951 2944 3135 33
Size: 1233 685 993 191 1121
Fragments arranged by size:
1233 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 314 314
Size: 4223
SfoI GGC'GCC
Cuts at: 295 571 1655 1903 295
Size: 276 1084 248 2615
Fragments arranged by size:
2615 1084 276 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
SmaI CCC'GGG
Cuts at: 1245 1245
Size: 4223
SmlI C'TyrA_G
Cuts at: 1378 2293 2785 3047 1378
Size: 915 492 262 2554
Fragments arranged by size:
2554 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4223
SphI G_CATG'C
Cuts at: 1041 1276 1776 1041
Size: 235 500 3488
Fragments arranged by size:
3488 500 235
StyI C'CwwG_G
Cuts at: 1148 1357 1711 1148
Size: 209 354 3660
Fragments arranged by size:
3660 354 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 601 1806 1939 2398 2607 601
Size: 1205 133 459 209 2217
Fragments arranged by size:
2217 1205 459 209 133
TatI w'GTAC_w
Cuts at: 776 3738 776
Size: 2962 1261
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3740 3772 3740
Size: 32 4191
Tth111I GACn'n_nGTC
Cuts at: 2020 2020
Size: 4223
XbaI T'CTAG_A
Cuts at: 1254 1254
Size: 4223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1718 1718
Size: 4223
XmaI C'CCGG_G
Cuts at: 1243 1243
Size: 4223
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NspI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI