Restriction map for
pF1KB4553
(Circular) (Six-base) MAPSORT of: pF1KB4553.seq Check: 8549 from: 1 to: 3548
pF1KB4553 3548 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 586 586
Size: 3548
Acc65I G'GTAC_C
Cuts at: 564 564
Size: 3548
AclI AA'CG_TT
Cuts at: 3388 3388
Size: 3548
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1373 1805 2552 2820 1373
Size: 432 747 268 2101
Fragments arranged by size:
2101 747 432 268
AflIII A'CryG_T
Cuts at: 900 2004 2981 900
Size: 1104 977 1467
Fragments arranged by size:
1467 1104 977
AgeI A'CCGG_T
Cuts at: 1936 1936
Size: 3548
AlwNI CAG_nnn'CTG
Cuts at: 853 2420 853
Size: 1567 1981
ApaI G_GGCC'C
Cuts at: 136 136
Size: 3548
ApaLI G'TGCA_C
Cuts at: 2318 2318
Size: 3548
ApoI r'AATT_y
Cuts at: 552 2901 3157 552
Size: 2349 256 943
Fragments arranged by size:
2349 943 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3548
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3548
AvaI C'yCGr_G
Cuts at: 568 568
Size: 3548
BamHI G'GATC_C
Cuts at: 573 573
Size: 3548
BanI G'GyrC_C
Cuts at: 564 978 1226 1261 564
Size: 414 248 35 2851
Fragments arranged by size:
2851 414 248 35
BanII G_rGCy'C
Cuts at: 128 136 270 562 1592 128
Size: 8 134 292 1030 2084
Fragments arranged by size:
2084 1030 292 134 8
BbeI G_GCGC'C
Cuts at: 982 1230 982
Size: 248 3300
BciVI GTATCCnnnnn_n'
Cuts at: 1446 2207 3548 1446
Size: 761 1341 1446
Fragments arranged by size:
1446 1341 761
BclI T'GATC_A
Cuts at: 1068 1068
Size: 3548
BglII A'GATC_T
Cuts at: 1063 3100 1063
Size: 2037 1511
BlpI GC'TnA_GC
Cuts at: 160 660 160
Size: 500 3048
Bme1580I G_kGCm'C
Cuts at: 136 243 374 1173 1266 2322 136
Size: 107 131 799 93 1056 1362
Fragments arranged by size:
1362 1056 799 131 107 93
BmrI ACTGGGnnnn_n'
Cuts at: 131 194 760 841 1176 3320 131
Size: 63 566 81 335 2144 359
Fragments arranged by size:
2144 566 359 335 81 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1915 1915
Size: 3548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 1603 2095 2393 724
Size: 879 492 298 1879
Fragments arranged by size:
1879 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3252 3252
Size: 3548
BsaAI yAC'GTr
Cuts at: 901 901
Size: 3548
BsaBI GATnn'nnATC
Cuts at: 6 1062 3099 6
Size: 1056 2037 455
Fragments arranged by size:
2037 1056 455
BsaHI Gr'CG_yC
Cuts at: 979 1227 1929 3425 979
Size: 248 702 1496 1102
Fragments arranged by size:
1496 1102 702 248
BsaWI w'CCGG_w
Cuts at: 737 1258 1936 2210 2357 2768 737
Size: 521 678 274 147 411 1517
Fragments arranged by size:
1517 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 180 210 180
Size: 30 3518
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 260 260
Size: 3548
BseYI C'CCAG_C
Cuts at: 973 1731 2308 2804 973
Size: 758 577 496 1717
Fragments arranged by size:
1717 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1090 1136 2344 104
Size: 986 46 1208 1308
Fragments arranged by size:
1308 1208 986 46
BsiHKAI G_wGCw'C
Cuts at: 562 1340 1530 2322 562
Size: 778 190 792 1788
Fragments arranged by size:
1788 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 128 136 243 270 374 562 1173 1266 1340
Size: 8 107 27 104 188 611 93 74
Cuts at: 1340 1530 1592 2322 128
Size: 190 62 730 1354
Fragments arranged by size:
1354 730 611 190 188 107 104 93
74 62 27 8
BspEI T'CCGG_A
Cuts at: 2768 2768
Size: 3548
BspHI T'CATG_A
Cuts at: 2724 2724
Size: 3548
BspMI ACCTGCnnnn'nnnn_
Cuts at: 598 812 1114 1495 598
Size: 214 302 381 2651
Fragments arranged by size:
2651 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1840 1894 3378 3543 50
Size: 1790 54 1484 165 55
Fragments arranged by size:
1790 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 524 1460 524
Size: 936 2612
BsrFI r'CCGG_y
Cuts at: 1546 1727 1936 1546
Size: 181 209 3158
Fragments arranged by size:
3158 209 181
BssSI C'ACGA_G
Cuts at: 1819 2177 1819
Size: 358 3190
BstAPI GCAn_nnn'nTGC
Cuts at: 378 925 378
Size: 547 3001
BstBI TT'CG_AA
Cuts at: 1909 1909
Size: 3548
BstXI CCAn_nnnn'nTGG
Cuts at: 420 519 420
Size: 99 3449
BstYI r'GATC_y
Cuts at: 1 573 1040 1063 1398 1644 2656 2742 2754
Size: 572 467 23 335 246 1012 86 12
Cuts at: 2754 3100 1
Size: 346 449
Fragments arranged by size:
1012 572 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3548
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1498 1679 1842 1498
Size: 181 163 3204
Fragments arranged by size:
3204 181 163
BtsI GCAGTG_nn'
Cuts at: 372 791 372
Size: 419 3129
DraI TTT'AAA
Cuts at: 547 547
Size: 3548
DrdI GACnn_nn'nnGTC
Cuts at: 1254 2112 1254
Size: 858 2690
EaeI y'GGCC_r
Cuts at: 332 521 1133 1307 1698 1725 3070 332
Size: 189 612 174 391 27 1345 810
Fragments arranged by size:
1345 810 612 391 189 174 27
EagI C'GGCC_G
Cuts at: 1133 1133
Size: 3548
EarI CTCTTCn'nnn_
Cuts at: 1571 1781 1571
Size: 210 3338
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1756 2066 2212 3358 1756
Size: 310 146 1146 1946
Fragments arranged by size:
1946 1146 310 146
EcoICRI GAG'CTC
Cuts at: 560 560
Size: 3548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1373 1805 2552 2820 1373
Size: 432 747 268 2101
Fragments arranged by size:
2101 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 430 430
Size: 3548
EcoO109I rG'GnC_Cy
Cuts at: 132 133 687 132
Size: 1 554 2993
Fragments arranged by size:
2993 554 1
EcoRI G'AATT_C
Cuts at: 552 552
Size: 3548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1567 1567
Size: 3548
FspI TGC'GCA
Cuts at: 490 1329 490
Size: 839 2709
HaeII r_GCGC'y
Cuts at: 361 982 1230 2252 3231 361
Size: 621 248 1022 979 678
Fragments arranged by size:
1022 979 678 621 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1390 1420 1422 1452 1390
Size: 30 2 30 3486
Fragments arranged by size:
3486 30 30 2
HincII GTy'rAC
Cuts at: 587 587
Size: 3548
KasI G'GCGC_C
Cuts at: 978 1226 978
Size: 248 3300
KpnI G_GTAC'C
Cuts at: 568 568
Size: 3548
MluI A'CGCG_T
Cuts at: 2981 2981
Size: 3548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 437 2219 2403 437
Size: 1782 184 1582
Fragments arranged by size:
1782 1582 184
MscI TGG'CCA
Cuts at: 334 523 1309 334
Size: 189 786 2573
Fragments arranged by size:
2573 786 189
MslI CAynn'nnrTG
Cuts at: 1537 1664 1537
Size: 127 3421
MspA1I CmG'CkG
Cuts at: 659 973 1333 2346 2591 659
Size: 314 360 1013 245 1616
Fragments arranged by size:
1616 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1729 1729
Size: 3548
NarI GG'CG_CC
Cuts at: 979 1227 979
Size: 248 3300
NcoI C'CATG_G
Cuts at: 107 107
Size: 3548
NgoMIV G'CCGG_C
Cuts at: 1727 1727
Size: 3548
NspI r_CATG'y
Cuts at: 381 601 1101 2008 381
Size: 220 500 907 1921
Fragments arranged by size:
1921 907 500 220
PasI CC'CwG_GG
Cuts at: 243 243
Size: 3548
PciI A'CATG_T
Cuts at: 2004 2004
Size: 3548
PmeI GTTT'AAAC
Cuts at: 547 547
Size: 3548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2687 2719 2687
Size: 32 3516
PspOMI G'GGCC_C
Cuts at: 132 132
Size: 3548
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 241 273 241
Size: 32 3516
PstI C_TGCA'G
Cuts at: 595 1280 595
Size: 685 2863
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3548
PvuII CAG'CTG
Cuts at: 973 1333 973
Size: 360 3188
RsrII CG'GwC_CG
Cuts at: 1743 1743
Size: 3548
SacI G_AGCT'C
Cuts at: 562 562
Size: 3548
SalI G'TCGA_C
Cuts at: 585 585
Size: 3548
SapI GCTCTTCn'nnn_
Cuts at: 1571 1781 1571
Size: 210 3338
SbfI CC_TGCA'GG
Cuts at: 595 595
Size: 3548
ScaI AGT'ACT
Cuts at: 3065 3065
Size: 3548
SfcI C'TryA_G
Cuts at: 33 591 1276 2269 2460 33
Size: 558 685 993 191 1121
Fragments arranged by size:
1121 993 685 558 191
SfoI GGC'GCC
Cuts at: 980 1228 980
Size: 248 3300
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3548
SmaI CCC'GGG
Cuts at: 570 570
Size: 3548
SmlI C'TyrA_G
Cuts at: 703 1618 2110 2372 703
Size: 915 492 262 1879
Fragments arranged by size:
1879 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3548
SphI G_CATG'C
Cuts at: 381 601 1101 381
Size: 220 500 2828
Fragments arranged by size:
2828 500 220
StyI C'CwwG_G
Cuts at: 107 512 682 1036 107
Size: 405 170 354 2619
Fragments arranged by size:
2619 405 354 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 486 1131 1264 1723 1932 486
Size: 645 133 459 209 2102
Fragments arranged by size:
2102 645 459 209 133
TatI w'GTAC_w
Cuts at: 3063 3063
Size: 3548
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 180 212 3065 3097 180
Size: 32 2853 32 631
Fragments arranged by size:
2853 631 32 32
Tth111I GACn'n_nGTC
Cuts at: 1345 1345
Size: 3548
XbaI T'CTAG_A
Cuts at: 579 579
Size: 3548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1043 1043
Size: 3548
XmaI C'CCGG_G
Cuts at: 568 568
Size: 3548
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI
PshAI PsiI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI