Restriction map for
pF1KB4518
(Circular) (Six-base) MAPSORT of: pF1KB4518.seq Check: 5972 from: 1 to: 4127
pF1KB4518 4127 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 436 436
Size: 4127
AccI GT'mk_AC
Cuts at: 1165 1165
Size: 4127
Acc65I G'GTAC_C
Cuts at: 1143 1143
Size: 4127
AclI AA'CG_TT
Cuts at: 3967 3967
Size: 4127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1952 2384 3131 3399 1952
Size: 432 747 268 2680
Fragments arranged by size:
2680 747 432 268
AflIII A'CryG_T
Cuts at: 1479 2583 3560 1479
Size: 1104 977 2046
Fragments arranged by size:
2046 1104 977
AgeI A'CCGG_T
Cuts at: 2515 2515
Size: 4127
AhdI GACnn_n'nnGTC
Cuts at: 330 330
Size: 4127
AlwNI CAG_nnn'CTG
Cuts at: 1432 2999 1432
Size: 1567 2560
ApaLI G'TGCA_C
Cuts at: 2897 2897
Size: 4127
ApoI r'AATT_y
Cuts at: 844 1131 3480 3736 844
Size: 287 2349 256 1235
Fragments arranged by size:
2349 1235 287 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4127
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
AvaI C'yCGr_G
Cuts at: 278 464 1147 278
Size: 186 683 3258
Fragments arranged by size:
3258 683 186
BamHI G'GATC_C
Cuts at: 1152 1152
Size: 4127
BanI G'GyrC_C
Cuts at: 1143 1557 1805 1840 1143
Size: 414 248 35 3430
Fragments arranged by size:
3430 414 248 35
BanII G_rGCy'C
Cuts at: 1141 2171 1141
Size: 1030 3097
BbeI G_GCGC'C
Cuts at: 1561 1809 1561
Size: 248 3879
BbsI GAAGACnn'nnnn_
Cuts at: 126 126
Size: 4127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 694 728 694
Size: 34 4093
BciVI GTATCCnnnnn_n'
Cuts at: 2025 2786 4127 2025
Size: 761 1341 2025
Fragments arranged by size:
2025 1341 761
BclI T'GATC_A
Cuts at: 350 1647 350
Size: 1297 2830
BglII A'GATC_T
Cuts at: 650 1642 3679 650
Size: 992 2037 1098
Fragments arranged by size:
2037 1098 992
BlpI GC'TnA_GC
Cuts at: 1239 1239
Size: 4127
Bme1580I G_kGCm'C
Cuts at: 176 1752 1845 2901 176
Size: 1576 93 1056 1402
Fragments arranged by size:
1576 1402 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 135 369 1339 1420 1755 3899 135
Size: 234 970 81 335 2144 363
Fragments arranged by size:
2144 970 363 335 234 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 4127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 1044 1049 667
Size: 377 5 3745
Fragments arranged by size:
3745 377 5
Bpu10I CC'TnA_GC
Cuts at: 811 811
Size: 4127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1303 2182 2674 2972 1303
Size: 879 492 298 2458
Fragments arranged by size:
2458 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 662 3831 662
Size: 3169 958
BsaAI yAC'GTr
Cuts at: 1480 1480
Size: 4127
BsaBI GATnn'nnATC
Cuts at: 6 1641 3678 6
Size: 1635 2037 455
Fragments arranged by size:
2037 1635 455
BsaHI Gr'CG_yC
Cuts at: 1558 1806 2508 4004 1558
Size: 248 702 1496 1681
Fragments arranged by size:
1681 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1316 1837 2515 2789 2936 3347 1316
Size: 521 678 274 147 411 2096
Fragments arranged by size:
2096 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 161 191 1042 1072 161
Size: 30 851 30 3216
Fragments arranged by size:
3216 851 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 482 501 482
Size: 19 4108
BseYI C'CCAG_C
Cuts at: 316 1552 2310 2887 3383 316
Size: 1236 758 577 496 1060
Fragments arranged by size:
1236 1060 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 457
Size: 4127
BsiEI CG_ry'CG
Cuts at: 104 1669 1715 2923 104
Size: 1565 46 1208 1308
Fragments arranged by size:
1565 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1068 1141 1919 2109 2901 1068
Size: 73 778 190 792 2294
Fragments arranged by size:
2294 792 778 190 73
BsmI GAATG_Cn'
Cuts at: 694 694
Size: 4127
Bsp1286I G_dGCh'C
Cuts at: 176 1068 1141 1752 1845 1919 2109 2171 2901
Size: 892 73 611 93 74 190 62 730
Cuts at: 2901 176
Size: 1402
Fragments arranged by size:
1402 892 730 611 190 93 74 73
62
BspEI T'CCGG_A
Cuts at: 3347 3347
Size: 4127
BspHI T'CATG_A
Cuts at: 386 500 3303 386
Size: 114 2803 1210
Fragments arranged by size:
2803 1210 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 436 1177 1391 1693 2074 436
Size: 741 214 302 381 2489
Fragments arranged by size:
2489 741 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2419 2473 3957 4122 50
Size: 2369 54 1484 165 55
Fragments arranged by size:
2369 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2039 2039
Size: 4127
BsrFI r'CCGG_y
Cuts at: 2125 2306 2515 2125
Size: 181 209 3737
Fragments arranged by size:
3737 209 181
BssSI C'ACGA_G
Cuts at: 344 2398 2756 344
Size: 2054 358 1715
Fragments arranged by size:
2054 1715 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1504 1504
Size: 4127
BstBI TT'CG_AA
Cuts at: 2488 2488
Size: 4127
BstEII G'GTnAC_C
Cuts at: 273 273
Size: 4127
BstXI CCAn_nnnn'nTGG
Cuts at: 210 885 1068 210
Size: 675 183 3269
Fragments arranged by size:
3269 675 183
BstYI r'GATC_y
Cuts at: 1 650 1152 1619 1642 1977 2223 3235 3321
Size: 649 502 467 23 335 246 1012 86
Cuts at: 3321 3333 3679 1
Size: 12 346 449
Fragments arranged by size:
1012 649 502 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 250 250
Size: 4127
BtgI C'CryG_G
Cuts at: 836 836
Size: 4127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2077 2258 2421 2077
Size: 181 163 3783
Fragments arranged by size:
3783 181 163
BtsI GCAGTG_nn'
Cuts at: 546 1370 546
Size: 824 3303
DraI TTT'AAA
Cuts at: 1126 1126
Size: 4127
DrdI GACnn_nn'nnGTC
Cuts at: 1833 2691 1833
Size: 858 3269
EaeI y'GGCC_r
Cuts at: 977 1712 1886 2277 2304 3649 977
Size: 735 174 391 27 1345 1455
Fragments arranged by size:
1455 1345 735 391 174 27
EagI C'GGCC_G
Cuts at: 1712 1712
Size: 4127
EarI CTCTTCn'nnn_
Cuts at: 163 2150 2360 163
Size: 1987 210 1930
Fragments arranged by size:
1987 1930 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2335 2645 2791 3937 2335
Size: 310 146 1146 2525
Fragments arranged by size:
2525 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1139 1139
Size: 4127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 1044 1049 1952 2384 3131 3399 667
Size: 377 5 903 432 747 268 1395
Fragments arranged by size:
1395 903 747 432 377 268 5
EcoO109I rG'GnC_Cy
Cuts at: 1091 1266 1091
Size: 175 3952
EcoRI G'AATT_C
Cuts at: 844 1131 844
Size: 287 3840
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 4127
FspI TGC'GCA
Cuts at: 181 1908 181
Size: 1727 2400
HaeII r_GCGC'y
Cuts at: 245 1561 1809 2831 3810 245
Size: 1316 248 1022 979 562
Fragments arranged by size:
1316 1022 979 562 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 316 348 432 464 1969 1999 2001 2031 316
Size: 32 84 32 1505 30 2 30 2412
Fragments arranged by size:
2412 1505 84 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1166 1166
Size: 4127
HindIII A'AGCT_T
Cuts at: 988 988
Size: 4127
KasI G'GCGC_C
Cuts at: 1557 1805 1557
Size: 248 3879
KpnI G_GTAC'C
Cuts at: 1147 1147
Size: 4127
MluI A'CGCG_T
Cuts at: 3560 3560
Size: 4127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2798 2982 2798
Size: 184 3943
MscI TGG'CCA
Cuts at: 979 1888 979
Size: 909 3218
MslI CAynn'nnrTG
Cuts at: 523 2116 2243 523
Size: 1593 127 2407
Fragments arranged by size:
2407 1593 127
MspA1I CmG'CkG
Cuts at: 185 1238 1552 1912 2925 3170 185
Size: 1053 314 360 1013 245 1142
Fragments arranged by size:
1142 1053 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2308 2308
Size: 4127
NarI GG'CG_CC
Cuts at: 1558 1806 1558
Size: 248 3879
NcoI C'CATG_G
Cuts at: 836 836
Size: 4127
NgoMIV G'CCGG_C
Cuts at: 2306 2306
Size: 4127
NspI r_CATG'y
Cuts at: 921 1180 1680 2587 921
Size: 259 500 907 2461
Fragments arranged by size:
2461 907 500 259
PciI A'CATG_T
Cuts at: 2583 2583
Size: 4127
PflMI CCAn_nnn'nTGG
Cuts at: 399 399
Size: 4127
PmeI GTTT'AAAC
Cuts at: 1126 1126
Size: 4127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 4095
PpuMI rG'GwC_Cy
Cuts at: 1091 1091
Size: 4127
PshAI GACnn'nnGTC
Cuts at: 416 600 416
Size: 184 3943
PstI C_TGCA'G
Cuts at: 831 1174 1859 831
Size: 343 685 3099
Fragments arranged by size:
3099 685 343
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4127
PvuII CAG'CTG
Cuts at: 1552 1912 1552
Size: 360 3767
RsrII CG'GwC_CG
Cuts at: 225 2322 225
Size: 2097 2030
SacI G_AGCT'C
Cuts at: 1141 1141
Size: 4127
SalI G'TCGA_C
Cuts at: 1164 1164
Size: 4127
SanDI GG'GwC_CC
Cuts at: 1091 1091
Size: 4127
SapI GCTCTTCn'nnn_
Cuts at: 163 2150 2360 163
Size: 1987 210 1930
Fragments arranged by size:
1987 1930 210
SbfI CC_TGCA'GG
Cuts at: 1174 1174
Size: 4127
ScaI AGT'ACT
Cuts at: 3644 3644
Size: 4127
SexAI A'CCwGG_T
Cuts at: 868 868
Size: 4127
SfcI C'TryA_G
Cuts at: 33 827 1170 1855 2848 3039 33
Size: 794 343 685 993 191 1121
Fragments arranged by size:
1121 993 794 685 343 191
SfoI GGC'GCC
Cuts at: 1559 1807 1559
Size: 248 3879
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
SmaI CCC'GGG
Cuts at: 280 466 1149 280
Size: 186 683 3258
Fragments arranged by size:
3258 683 186
SmlI C'TyrA_G
Cuts at: 1282 2197 2689 2951 1282
Size: 915 492 262 2458
Fragments arranged by size:
2458 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4127
SphI G_CATG'C
Cuts at: 1180 1680 1180
Size: 500 3627
StyI C'CwwG_G
Cuts at: 836 1261 1615 836
Size: 425 354 3348
Fragments arranged by size:
3348 425 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 784 1710 1843 2302 2511 784
Size: 926 133 459 209 2400
Fragments arranged by size:
2400 926 459 209 133
TatI w'GTAC_w
Cuts at: 377 3642 377
Size: 3265 862
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 161 193 527 559 3644 3676 161
Size: 32 334 32 3085 32 612
Fragments arranged by size:
3085 612 334 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1924 1924
Size: 4127
XbaI T'CTAG_A
Cuts at: 1158 1158
Size: 4127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 587 1622 587
Size: 1035 3092
XmaI C'CCGG_G
Cuts at: 278 464 1147 278
Size: 186 683 3258
Fragments arranged by size:
3258 683 186
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BsiWI
BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PsiI PspOMI
PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI