Restriction map for
pF1KB4501
(Circular) (Six-base) MAPSORT of: pF1KB4501.seq Check: 1518 from: 1 to: 3707
pF1KB4501 3707 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 745 745
Size: 3707
Acc65I G'GTAC_C
Cuts at: 723 723
Size: 3707
AclI AA'CG_TT
Cuts at: 3547 3547
Size: 3707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1532 1964 2711 2979 1532
Size: 432 747 268 2260
Fragments arranged by size:
2260 747 432 268
AflIII A'CryG_T
Cuts at: 1059 2163 3140 1059
Size: 1104 977 1626
Fragments arranged by size:
1626 1104 977
AgeI A'CCGG_T
Cuts at: 2095 2095
Size: 3707
AlwNI CAG_nnn'CTG
Cuts at: 1012 2579 1012
Size: 1567 2140
ApaLI G'TGCA_C
Cuts at: 2477 2477
Size: 3707
ApoI r'AATT_y
Cuts at: 412 711 3060 3316 412
Size: 299 2349 256 803
Fragments arranged by size:
2349 803 299 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3707
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
AvaI C'yCGr_G
Cuts at: 542 727 542
Size: 185 3522
BamHI G'GATC_C
Cuts at: 732 732
Size: 3707
BanI G'GyrC_C
Cuts at: 723 1137 1385 1420 723
Size: 414 248 35 3010
Fragments arranged by size:
3010 414 248 35
BanII G_rGCy'C
Cuts at: 721 1751 721
Size: 1030 2677
BbeI G_GCGC'C
Cuts at: 1141 1389 1141
Size: 248 3459
BbsI GAAGACnn'nnnn_
Cuts at: 391 448 391
Size: 57 3650
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 197 231 197
Size: 34 3673
BciVI GTATCCnnnnn_n'
Cuts at: 1605 2366 3707 1605
Size: 761 1341 1605
Fragments arranged by size:
1605 1341 761
BclI T'GATC_A
Cuts at: 1227 1227
Size: 3707
BglII A'GATC_T
Cuts at: 669 1222 3259 669
Size: 553 2037 1117
Fragments arranged by size:
2037 1117 553
BlpI GC'TnA_GC
Cuts at: 819 819
Size: 3707
Bme1580I G_kGCm'C
Cuts at: 1332 1425 2481 1332
Size: 93 1056 2558
Fragments arranged by size:
2558 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 220 919 1000 1335 3479 220
Size: 699 81 335 2144 448
Fragments arranged by size:
2144 699 448 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 648 2074 648
Size: 1426 2281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 883 1762 2254 2552 271
Size: 612 879 492 298 1426
Fragments arranged by size:
1426 879 612 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 471 3411 471
Size: 2940 767
BsaAI yAC'GTr
Cuts at: 1060 1060
Size: 3707
BsaBI GATnn'nnATC
Cuts at: 6 1221 3258 6
Size: 1215 2037 455
Fragments arranged by size:
2037 1215 455
BsaHI Gr'CG_yC
Cuts at: 1138 1386 2088 3584 1138
Size: 248 702 1496 1261
Fragments arranged by size:
1496 1261 702 248
BsaWI w'CCGG_w
Cuts at: 896 1417 2095 2369 2516 2927 896
Size: 521 678 274 147 411 1676
Fragments arranged by size:
1676 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 684 714 684
Size: 30 3677
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 415 445 505 705 415
Size: 30 60 200 3417
Fragments arranged by size:
3417 200 60 30
BseYI C'CCAG_C
Cuts at: 1132 1890 2467 2963 1132
Size: 758 577 496 1876
Fragments arranged by size:
1876 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 526
Size: 3707
BsiEI CG_ry'CG
Cuts at: 104 1249 1295 2503 104
Size: 1145 46 1208 1308
Fragments arranged by size:
1308 1208 1145 46
BsiHKAI G_wGCw'C
Cuts at: 721 1499 1689 2481 721
Size: 778 190 792 1947
Fragments arranged by size:
1947 792 778 190
BsmI GAATG_Cn'
Cuts at: 367 367
Size: 3707
Bsp1286I G_dGCh'C
Cuts at: 721 1332 1425 1499 1689 1751 2481 721
Size: 611 93 74 190 62 730 1947
Fragments arranged by size:
1947 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2927 2927
Size: 3707
BspHI T'CATG_A
Cuts at: 2883 2883
Size: 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 572 757 971 1273 1654 572
Size: 185 214 302 381 2625
Fragments arranged by size:
2625 381 302 214 185
BsrBI CCG'CTC
Cuts at: 50 1999 2053 3537 3702 50
Size: 1949 54 1484 165 55
Fragments arranged by size:
1949 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1619 1619
Size: 3707
BsrFI r'CCGG_y
Cuts at: 1705 1886 2095 1705
Size: 181 209 3317
Fragments arranged by size:
3317 209 181
BssSI C'ACGA_G
Cuts at: 237 552 1978 2336 237
Size: 315 1426 358 1608
Fragments arranged by size:
1608 1426 358 315
BstAPI GCAn_nnn'nTGC
Cuts at: 373 1084 373
Size: 711 2996
BstBI TT'CG_AA
Cuts at: 2068 2068
Size: 3707
BstXI CCAn_nnnn'nTGG
Cuts at: 148 148
Size: 3707
BstYI r'GATC_y
Cuts at: 1 482 669 732 1199 1222 1557 1803 2815
Size: 481 187 63 467 23 335 246 1012
Cuts at: 2815 2901 2913 3259 1
Size: 86 12 346 449
Fragments arranged by size:
1012 481 467 449 346 335 246 187
86 63 23 12
Bsu36I CC'TnA_GG
Cuts at: 161 649 161
Size: 488 3219
BtgI C'CryG_G
Cuts at: 514 514
Size: 3707
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1657 1838 2001 1657
Size: 181 163 3363
Fragments arranged by size:
3363 181 163
BtsI GCAGTG_nn'
Cuts at: 950 950
Size: 3707
DraI TTT'AAA
Cuts at: 706 706
Size: 3707
DrdI GACnn_nn'nnGTC
Cuts at: 1413 2271 1413
Size: 858 2849
EaeI y'GGCC_r
Cuts at: 177 1292 1466 1857 1884 3229 177
Size: 1115 174 391 27 1345 655
Fragments arranged by size:
1345 1115 655 391 174 27
EagI C'GGCC_G
Cuts at: 1292 1292
Size: 3707
EarI CTCTTCn'nnn_
Cuts at: 1730 1940 1730
Size: 210 3497
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1915 2225 2371 3517 1915
Size: 310 146 1146 2105
Fragments arranged by size:
2105 1146 310 146
EcoICRI GAG'CTC
Cuts at: 719 719
Size: 3707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1532 1964 2711 2979 1532
Size: 432 747 268 2260
Fragments arranged by size:
2260 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 846 846
Size: 3707
EcoRI G'AATT_C
Cuts at: 412 711 412
Size: 299 3408
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1726 1726
Size: 3707
FspI TGC'GCA
Cuts at: 1488 1488
Size: 3707
HaeII r_GCGC'y
Cuts at: 1141 1389 2411 3390 1141
Size: 248 1022 979 1458
Fragments arranged by size:
1458 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 189 221 661 693 1549 1579 1581 1611 189
Size: 32 440 32 856 30 2 30 2285
Fragments arranged by size:
2285 856 440 32 32 30 30 2
HincII GTy'rAC
Cuts at: 452 746 452
Size: 294 3413
KasI G'GCGC_C
Cuts at: 1137 1385 1137
Size: 248 3459
KpnI G_GTAC'C
Cuts at: 727 727
Size: 3707
MluI A'CGCG_T
Cuts at: 3140 3140
Size: 3707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2378 2562 2378
Size: 184 3523
MscI TGG'CCA
Cuts at: 179 1468 179
Size: 1289 2418
MslI CAynn'nnrTG
Cuts at: 353 1696 1823 353
Size: 1343 127 2237
Fragments arranged by size:
2237 1343 127
MspA1I CmG'CkG
Cuts at: 818 1132 1492 2505 2750 818
Size: 314 360 1013 245 1775
Fragments arranged by size:
1775 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1888 1888
Size: 3707
NarI GG'CG_CC
Cuts at: 1138 1386 1138
Size: 248 3459
NgoMIV G'CCGG_C
Cuts at: 1886 1886
Size: 3707
NspI r_CATG'y
Cuts at: 760 1260 2167 760
Size: 500 907 2300
Fragments arranged by size:
2300 907 500
PciI A'CATG_T
Cuts at: 2163 2163
Size: 3707
PmeI GTTT'AAAC
Cuts at: 706 706
Size: 3707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2846 2878 2846
Size: 32 3675
PstI C_TGCA'G
Cuts at: 569 754 1439 569
Size: 185 685 2837
Fragments arranged by size:
2837 685 185
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3707
PvuII CAG'CTG
Cuts at: 1132 1492 1132
Size: 360 3347
RsrII CG'GwC_CG
Cuts at: 1902 1902
Size: 3707
SacI G_AGCT'C
Cuts at: 721 721
Size: 3707
SalI G'TCGA_C
Cuts at: 744 744
Size: 3707
SapI GCTCTTCn'nnn_
Cuts at: 1730 1940 1730
Size: 210 3497
SbfI CC_TGCA'GG
Cuts at: 569 754 569
Size: 185 3522
ScaI AGT'ACT
Cuts at: 3224 3224
Size: 3707
SexAI A'CCwGG_T
Cuts at: 445 445
Size: 3707
SfcI C'TryA_G
Cuts at: 33 565 750 1435 2428 2619 33
Size: 532 185 685 993 191 1121
Fragments arranged by size:
1121 993 685 532 191 185
SfoI GGC'GCC
Cuts at: 1139 1387 1139
Size: 248 3459
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
SmaI CCC'GGG
Cuts at: 729 729
Size: 3707
SmlI C'TyrA_G
Cuts at: 286 862 1777 2269 2531 286
Size: 576 915 492 262 1462
Fragments arranged by size:
1462 915 576 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3707
SphI G_CATG'C
Cuts at: 760 1260 760
Size: 500 3207
StyI C'CwwG_G
Cuts at: 600 841 1195 600
Size: 241 354 3112
Fragments arranged by size:
3112 354 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1290 1423 1882 2091 1290
Size: 133 459 209 2906
Fragments arranged by size:
2906 459 209 133
TatI w'GTAC_w
Cuts at: 609 662 3222 609
Size: 53 2560 1094
Fragments arranged by size:
2560 1094 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 684 716 3224 3256 684
Size: 32 2508 32 1135
Fragments arranged by size:
2508 1135 32 32
Tth111I GACn'n_nGTC
Cuts at: 447 1504 447
Size: 1057 2650
XbaI T'CTAG_A
Cuts at: 738 738
Size: 3707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1202 1202
Size: 3707
XmaI C'CCGG_G
Cuts at: 727 727
Size: 3707
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI