Restriction map for
pF1KB4495
(Circular) (Six-base) MAPSORT of: pF1KB4495.seq Check: 5198 from: 1 to: 6002
pF1KB4495 6002 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 930 930
Size: 6002
AccI GT'mk_AC
Cuts at: 251 3040 251
Size: 2789 3213
Acc65I G'GTAC_C
Cuts at: 3018 3018
Size: 6002
AclI AA'CG_TT
Cuts at: 5842 5842
Size: 6002
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 807 1819 2542 2614 2949 3827 4259 4612
Size: 458 1012 723 72 335 878 432 353
Cuts at: 4612 5274 349
Size: 662 1077
Fragments arranged by size:
1077 1012 878 723 662 458 432 353
335 72
AfeI AGC'GCT
Cuts at: 835 2674 2899 835
Size: 1839 225 3938
Fragments arranged by size:
3938 1839 225
AflIII A'CryG_T
Cuts at: 3354 5154 5435 3354
Size: 1800 281 3921
Fragments arranged by size:
3921 1800 281
AleI CACnn'nnGTG
Cuts at: 2320 2320
Size: 6002
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1144 1619 2338 1144
Size: 475 719 4808
Fragments arranged by size:
4808 719 475
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 845 877 845
Size: 32 5970
AlwNI CAG_nnn'CTG
Cuts at: 1220 1353 2340 2411 2613 2856 3307 4745 1220
Size: 133 987 71 202 243 451 1438 2477
Fragments arranged by size:
2477 1438 987 451 243 202 133 71
ApaI G_GGCC'C
Cuts at: 2283 2305 2283
Size: 22 5980
ApaLI G'TGCA_C
Cuts at: 2248 4840 2248
Size: 2592 3410
ApoI r'AATT_y
Cuts at: 2353 3006 5355 5611 2353
Size: 653 2349 256 2744
Fragments arranged by size:
2744 2349 653 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6002
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6002
AvaI C'yCGr_G
Cuts at: 532 928 1132 2298 2305 2491 3022 532
Size: 396 204 1166 7 186 531 3512
Fragments arranged by size:
3512 1166 531 396 204 186 7
BamHI G'GATC_C
Cuts at: 122 3027 122
Size: 2905 3097
BanI G'GyrC_C
Cuts at: 1644 3018 3432 3680 3715 1644
Size: 1374 414 248 35 3931
Fragments arranged by size:
3931 1374 414 248 35
BanII G_rGCy'C
Cuts at: 1205 1448 1859 2283 2305 2492 3016 4046 1205
Size: 243 411 424 22 187 524 1030 3161
Fragments arranged by size:
3161 1030 524 424 411 243 187 22
BbeI G_GCGC'C
Cuts at: 3436 3684 3436
Size: 248 5754
BbvCI CC'TCA_GC
Cuts at: 1934 1934
Size: 6002
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 738 772 738
Size: 34 5968
BciVI GTATCCnnnnn_n'
Cuts at: 3900 4956 6002 3900
Size: 1056 1046 3900
Fragments arranged by size:
3900 1056 1046
BclI T'GATC_A
Cuts at: 3522 3522
Size: 6002
BglI GCCn_nnn'nGGC
Cuts at: 112 804 1663 1968 112
Size: 692 859 305 4146
Fragments arranged by size:
4146 859 692 305
BglII A'GATC_T
Cuts at: 1166 3517 5554 1166
Size: 2351 2037 1614
Fragments arranged by size:
2351 2037 1614
BlpI GC'TnA_GC
Cuts at: 116 726 1252 2164 2311 3114 116
Size: 610 526 912 147 803 3004
Fragments arranged by size:
3004 912 803 610 526 147
Bme1580I G_kGCm'C
Cuts at: 635 1133 1608 2252 2283 2305 3627 3720 4844
Size: 498 475 644 31 22 1322 93 1124
Cuts at: 4844 635
Size: 1793
Fragments arranged by size:
1793 1322 1124 644 498 475 93 31
22
BmgBI CAC'GTC
Cuts at: 1143 1143
Size: 6002
BmrI ACTGGGnnnn_n'
Cuts at: 230 1908 2924 3214 3295 3630 5774 230
Size: 1678 1016 290 81 335 2144 458
Fragments arranged by size:
2144 1678 1016 458 335 290 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4369 4369
Size: 6002
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 1219 1261 1282 1330 1462 2152 2350 2832
Size: 675 42 21 48 132 690 198 482
Cuts at: 2832 544
Size: 3714
Fragments arranged by size:
3714 690 675 482 198 132 48 42
21
Bpu10I CC'TnA_GC
Cuts at: 184 1934 184
Size: 1750 4252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1702 1969 2059 2077 3178 4057 4771 5069 1702
Size: 267 90 18 1101 879 714 298 2635
Fragments arranged by size:
2635 1101 879 714 298 267 90 18
BsaI GGTCTCn'nnnn_
Cuts at: 1150 1543 2712 5706 1150
Size: 393 1169 2994 1446
Fragments arranged by size:
2994 1446 1169 393
BsaAI yAC'GTr
Cuts at: 1128 1458 2363 2442 3355 1128
Size: 330 905 79 913 3775
Fragments arranged by size:
3775 913 905 330 79
BsaBI GATnn'nnATC
Cuts at: 6 292 3516 5553 6
Size: 286 3224 2037 455
Fragments arranged by size:
3224 2037 455 286
BsaHI Gr'CG_yC
Cuts at: 2429 2503 2726 3433 3681 4383 5879 2429
Size: 74 223 707 248 702 1496 2552
Fragments arranged by size:
2552 1496 707 702 248 223 74
BsaWI w'CCGG_w
Cuts at: 449 3191 3712 4390 4801 4948 5222 449
Size: 2742 521 678 411 147 274 1229
Fragments arranged by size:
2742 1229 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 406 436 406
Size: 30 5972
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 414 1371 1922 2185 414
Size: 957 551 263 4231
Fragments arranged by size:
4231 957 551 263
BseYI C'CCAG_C
Cuts at: 643 1160 1803 2287 2669 3427 4185 4850 5258
Size: 517 643 484 382 758 758 665 408
Cuts at: 5258 643
Size: 1387
Fragments arranged by size:
1387 758 758 665 643 517 484 408
382
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 1417 2842 454
Size: 963 1425 3614
Fragments arranged by size:
3614 1425 963
BsiEI CG_ry'CG
Cuts at: 104 3544 3590 4820 104
Size: 3440 46 1230 1286
Fragments arranged by size:
3440 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1205 1859 2252 2299 2317 2645 3016 3794 3984
Size: 654 393 47 18 328 371 778 190
Cuts at: 3984 4844 1205
Size: 860 2363
Fragments arranged by size:
2363 860 778 654 393 371 328 190
47 18
BsmBI CGTCTCn'nnnn_
Cuts at: 196 196
Size: 6002
Bsp1286I G_dGCh'C
Cuts at: 635 1133 1205 1448 1608 1859 2252 2283 2299
Size: 498 72 243 160 251 393 31 16
Cuts at: 2299 2305 2317 2492 2645 3016 3627 3720 3794
Size: 6 12 175 153 371 611 93 74
Cuts at: 3794 3984 4046 4844 635
Size: 190 62 798 1793
Fragments arranged by size:
1793 798 611 498 393 371 251 243
190 175 160 153 93 74 72 62
31 16 12 6
BspHI T'CATG_A
Cuts at: 4434 4434
Size: 6002
BspMI ACCTGCnnnn'nnnn_
Cuts at: 688 930 2135 2456 3052 3266 3568 3949 688
Size: 242 1205 321 596 214 302 381 2741
Fragments arranged by size:
2741 1205 596 381 321 302 242 214
BsrBI CCG'CTC
Cuts at: 50 130 1395 2175 2454 2496 4294 4348 5832
Size: 80 1265 780 279 42 1798 54 1484
Cuts at: 5832 5997 50
Size: 165 55
Fragments arranged by size:
1798 1484 1265 780 279 165 80 55
54 42
BsrDI GCAATG_nn'
Cuts at: 3914 3914
Size: 6002
BsrFI r'CCGG_y
Cuts at: 798 907 4000 4181 798
Size: 109 3093 181 2619
Fragments arranged by size:
3093 2619 181 109
BsrGI T'GTAC_A
Cuts at: 572 572
Size: 6002
BssSI C'ACGA_G
Cuts at: 2245 4273 4981 2245
Size: 2028 708 3266
Fragments arranged by size:
3266 2028 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2340 3379 2340
Size: 1039 4963
BstBI TT'CG_AA
Cuts at: 957 4363 957
Size: 3406 2596
BstXI CCAn_nnnn'nTGG
Cuts at: 408 522 1027 408
Size: 114 505 5383
Fragments arranged by size:
5383 505 114
BstYI r'GATC_y
Cuts at: 1 122 1166 1713 2336 3027 3494 3517 3852
Size: 121 1044 547 623 691 467 23 335
Cuts at: 3852 4098 4404 4416 4502 5554 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 1044 691 623 547 467 449 335
306 246 121 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1354 1354
Size: 6002
BtgI C'CryG_G
Cuts at: 107 1049 1473 2651 107
Size: 942 424 1178 3458
Fragments arranged by size:
3458 1178 942 424
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3952 4133 4296 3952
Size: 181 163 5658
Fragments arranged by size:
5658 181 163
BtsI GCAGTG_nn'
Cuts at: 3245 3245
Size: 6002
DraI TTT'AAA
Cuts at: 3001 3001
Size: 6002
DraIII CAC_nnn'GTG
Cuts at: 466 1505 466
Size: 1039 4963
DrdI GACnn_nn'nnGTC
Cuts at: 3708 5052 3708
Size: 1344 4658
EaeI y'GGCC_r
Cuts at: 131 1017 2699 3587 3761 4152 4179 5524 131
Size: 886 1682 888 174 391 27 1345 609
Fragments arranged by size:
1682 1345 888 886 609 391 174 27
EagI C'GGCC_G
Cuts at: 3587 3587
Size: 6002
EarI CTCTTCn'nnn_
Cuts at: 4025 4235 4025
Size: 210 5792
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4210 4952 5098 5812 4210
Size: 742 146 714 4400
Fragments arranged by size:
4400 742 714 146
EcoICRI GAG'CTC
Cuts at: 1203 1857 3014 1203
Size: 654 1157 4191
Fragments arranged by size:
4191 1157 654
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 544 807 1219 1261 1282 1330 1462 1819
Size: 195 263 412 42 21 48 132 357
Cuts at: 1819 2152 2350 2542 2614 2832 2949 3827 4259
Size: 333 198 192 72 218 117 878 432
Cuts at: 4259 4612 5274 349
Size: 353 662 1077
Fragments arranged by size:
1077 878 662 432 412 357 353 333
263 218 198 195 192 132 117 72
48 42 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2379 2379
Size: 6002
EcoO109I rG'GnC_Cy
Cuts at: 170 1217 1706 2279 2302 2384 2610 3141 170
Size: 1047 489 573 23 82 226 531 3031
Fragments arranged by size:
3031 1047 573 531 489 226 82 23
EcoRI G'AATT_C
Cuts at: 3006 3006
Size: 6002
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4021 4021
Size: 6002
FspI TGC'GCA
Cuts at: 3783 3783
Size: 6002
HaeII r_GCGC'y
Cuts at: 837 2418 2676 2901 3436 3684 4914 5685 837
Size: 1581 258 225 535 248 1230 771 1154
Fragments arranged by size:
1581 1230 1154 771 535 258 248 225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 471 503 3844 3874 3876 3906 471
Size: 32 3341 30 2 30 2567
Fragments arranged by size:
3341 2567 32 30 30 2
HincII GTy'rAC
Cuts at: 3041 3041
Size: 6002
KasI G'GCGC_C
Cuts at: 3432 3680 3432
Size: 248 5754
KpnI G_GTAC'C
Cuts at: 3022 3022
Size: 6002
MluI A'CGCG_T
Cuts at: 5435 5435
Size: 6002
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4761 4945 4761
Size: 184 5818
MscI TGG'CCA
Cuts at: 1019 2701 3763 1019
Size: 1682 1062 3258
Fragments arranged by size:
3258 1682 1062
MslI CAynn'nnrTG
Cuts at: 223 406 1126 1601 2320 3991 4118 223
Size: 183 720 475 719 1671 127 2107
Fragments arranged by size:
2107 1671 720 719 475 183 127
MspA1I CmG'CkG
Cuts at: 120 473 1197 1406 1431 1674 1797 2196 2411
Size: 353 724 209 25 243 123 399 215
Cuts at: 2411 3113 3427 3787 4571 4816 120
Size: 702 314 360 784 245 1306
Fragments arranged by size:
1306 784 724 702 399 360 353 314
245 243 215 209 123 25
NaeI GCC'GGC
Cuts at: 4183 4183
Size: 6002
NarI GG'CG_CC
Cuts at: 3433 3681 3433
Size: 248 5754
NcoI C'CATG_G
Cuts at: 107 1473 107
Size: 1366 4636
NdeI CA'TA_TG
Cuts at: 1380 1380
Size: 6002
NgoMIV G'CCGG_C
Cuts at: 4181 4181
Size: 6002
NspI r_CATG'y
Cuts at: 3055 3555 5158 3055
Size: 500 1603 3899
Fragments arranged by size:
3899 1603 500
PasI CC'CwG_GG
Cuts at: 1150 2433 1150
Size: 1283 4719
PciI A'CATG_T
Cuts at: 5154 5154
Size: 6002
PflMI CCAn_nnn'nTGG
Cuts at: 1005 1050 1505 2210 2613 2900 1005
Size: 45 455 705 403 287 4107
Fragments arranged by size:
4107 705 455 403 287 45
PfoI T'CCnGG_A
Cuts at: 2051 2630 2849 2051
Size: 579 219 5204
Fragments arranged by size:
5204 579 219
PmeI GTTT'AAAC
Cuts at: 3001 3001
Size: 6002
PmlI CAC'GTG
Cuts at: 1128 1128
Size: 6002
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4448 4480 4448
Size: 32 5970
PpuMI rG'GwC_Cy
Cuts at: 170 1217 2384 170
Size: 1047 1167 3788
Fragments arranged by size:
3788 1167 1047
PspOMI G'GGCC_C
Cuts at: 2279 2301 2279
Size: 22 5980
PstI C_TGCA'G
Cuts at: 1225 1405 1673 3049 3734 1225
Size: 180 268 1376 685 3493
Fragments arranged by size:
3493 1376 685 268 180
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6002
PvuII CAG'CTG
Cuts at: 1406 1431 1674 1797 2196 2411 3427 3787 1406
Size: 25 243 123 399 215 1016 360 3621
Fragments arranged by size:
3621 1016 399 360 243 215 123 25
RsrII CG'GwC_CG
Cuts at: 4197 4197
Size: 6002
SacI G_AGCT'C
Cuts at: 1205 1859 3016 1205
Size: 654 1157 4191
Fragments arranged by size:
4191 1157 654
SalI G'TCGA_C
Cuts at: 3039 3039
Size: 6002
SanDI GG'GwC_CC
Cuts at: 170 170
Size: 6002
SapI GCTCTTCn'nnn_
Cuts at: 4025 4235 4025
Size: 210 5792
SbfI CC_TGCA'GG
Cuts at: 3049 3049
Size: 6002
ScaI AGT'ACT
Cuts at: 672 5519 672
Size: 4847 1155
SfcI C'TryA_G
Cuts at: 33 840 1221 1401 1669 2657 3045 3730 4698
Size: 807 381 180 268 988 388 685 968
Cuts at: 4698 4889 33
Size: 191 1146
Fragments arranged by size:
1146 988 968 807 685 388 381 268
191 180
SfoI GGC'GCC
Cuts at: 3434 3682 3434
Size: 248 5754
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6002
SmaI CCC'GGG
Cuts at: 3024 3024
Size: 6002
SmlI C'TyrA_G
Cuts at: 1717 1984 2074 2092 3157 4072 4786 5048 1717
Size: 267 90 18 1065 915 714 262 2671
Fragments arranged by size:
2671 1065 915 714 267 262 90 18
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6002
SphI G_CATG'C
Cuts at: 3055 3555 3055
Size: 500 5502
StyI C'CwwG_G
Cuts at: 107 338 515 1473 2204 2702 3136 3490 107
Size: 231 177 958 731 498 434 354 2619
Fragments arranged by size:
2619 958 731 498 434 354 231 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1577 1662 1927 3585 3718 4177 4386 1577
Size: 85 265 1658 133 459 209 3193
Fragments arranged by size:
3193 1658 459 265 209 133 85
TatI w'GTAC_w
Cuts at: 572 670 2135 5517 572
Size: 98 1465 3382 1057
Fragments arranged by size:
3382 1465 1057 98
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5519 5551 5519
Size: 32 5970
Tth111I GACn'n_nGTC
Cuts at: 1417 3799 1417
Size: 2382 3620
XbaI T'CTAG_A
Cuts at: 3033 3033
Size: 6002
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 672 1147 2262 2718 3497 672
Size: 475 1115 456 779 3177
Fragments arranged by size:
3177 1115 779 475 456
XmaI C'CCGG_G
Cuts at: 3022 3022
Size: 6002
XmnI GAAnn'nnTTC
Cuts at: 2563 2563
Size: 6002
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AscI AvrII BaeI BbsI
BfrBI BmtI BsiWI BsmI BspEI BssHII BstEII BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI