Restriction map for
pF1KB4480
(Circular) (Six-base) MAPSORT of: pF1KB4480.seq Check: 4651 from: 1 to: 4190
pF1KB4480 4190 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 961 1228 961
Size: 267 3923
Acc65I G'GTAC_C
Cuts at: 1206 1206
Size: 4190
AclI AA'CG_TT
Cuts at: 4030 4030
Size: 4190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 2015 2447 3194 3462 515
Size: 1500 432 747 268 1243
Fragments arranged by size:
1500 1243 747 432 268
AflII C'TTAA_G
Cuts at: 841 841
Size: 4190
AflIII A'CryG_T
Cuts at: 1542 2646 3623 1542
Size: 1104 977 2109
Fragments arranged by size:
2109 1104 977
AgeI A'CCGG_T
Cuts at: 2578 2578
Size: 4190
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 855 887 855
Size: 32 4158
AlwNI CAG_nnn'CTG
Cuts at: 1495 3062 1495
Size: 1567 2623
ApaLI G'TGCA_C
Cuts at: 2960 2960
Size: 4190
ApoI r'AATT_y
Cuts at: 546 736 989 1114 1194 3543 3799 546
Size: 190 253 125 80 2349 256 937
Fragments arranged by size:
2349 937 256 253 190 125 80
AseI AT'TA_AT
Cuts at: 20 835 20
Size: 815 3375
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 1210 1210
Size: 4190
BamHI G'GATC_C
Cuts at: 1215 1215
Size: 4190
BanI G'GyrC_C
Cuts at: 449 1206 1620 1868 1903 449
Size: 757 414 248 35 2736
Fragments arranged by size:
2736 757 414 248 35
BanII G_rGCy'C
Cuts at: 1152 1204 2234 1152
Size: 52 1030 3108
Fragments arranged by size:
3108 1030 52
BbeI G_GCGC'C
Cuts at: 453 1624 1872 453
Size: 1171 248 2771
Fragments arranged by size:
2771 1171 248
BbsI GAAGACnn'nnnn_
Cuts at: 943 943
Size: 4190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4156
BciVI GTATCCnnnnn_n'
Cuts at: 2088 2849 4190 2088
Size: 761 1341 2088
Fragments arranged by size:
2088 1341 761
BclI T'GATC_A
Cuts at: 1710 1710
Size: 4190
BfrBI ATG'CAT
Cuts at: 832 832
Size: 4190
BglII A'GATC_T
Cuts at: 1705 3742 1705
Size: 2037 2153
BlpI GC'TnA_GC
Cuts at: 1302 1302
Size: 4190
Bme1580I G_kGCm'C
Cuts at: 1815 1908 2964 1815
Size: 93 1056 3041
Fragments arranged by size:
3041 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1402 1483 1818 3962 1402
Size: 81 335 2144 1630
Fragments arranged by size:
2144 1630 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 2245 2737 3035 1366
Size: 879 492 298 2521
Fragments arranged by size:
2521 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3894 3894
Size: 4190
BsaAI yAC'GTr
Cuts at: 816 1543 816
Size: 727 3463
BsaBI GATnn'nnATC
Cuts at: 6 1704 3741 6
Size: 1698 2037 455
Fragments arranged by size:
2037 1698 455
BsaHI Gr'CG_yC
Cuts at: 450 1621 1869 2571 4067 450
Size: 1171 248 702 1496 573
Fragments arranged by size:
1496 1171 702 573 248
BsaWI w'CCGG_w
Cuts at: 1379 1900 2578 2852 2999 3410 1379
Size: 521 678 274 147 411 2159
Fragments arranged by size:
2159 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 470 500 470
Size: 30 4160
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 818 1160 208
Size: 610 342 3238
Fragments arranged by size:
3238 610 342
BseYI C'CCAG_C
Cuts at: 940 1615 2373 2950 3446 940
Size: 675 758 577 496 1684
Fragments arranged by size:
1684 758 675 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 554 123
Size: 431 3759
BsiEI CG_ry'CG
Cuts at: 104 1732 1778 2986 104
Size: 1628 46 1208 1308
Fragments arranged by size:
1628 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 200 1152 1204 1982 2172 2964 200
Size: 952 52 778 190 792 1426
Fragments arranged by size:
1426 952 792 778 190 52
BsmI GAATG_Cn'
Cuts at: 554 1109 554
Size: 555 3635
BsmBI CGTCTCn'nnnn_
Cuts at: 199 199
Size: 4190
Bsp1286I G_dGCh'C
Cuts at: 200 1152 1204 1815 1908 1982 2172 2234 2964
Size: 952 52 611 93 74 190 62 730
Cuts at: 2964 200
Size: 1426
Fragments arranged by size:
1426 952 730 611 190 93 74 62
52
BspEI T'CCGG_A
Cuts at: 3410 3410
Size: 4190
BspHI T'CATG_A
Cuts at: 471 3366 471
Size: 2895 1295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 509 1240 1454 1756 2137 509
Size: 731 214 302 381 2562
Fragments arranged by size:
2562 731 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2482 2536 4020 4185 50
Size: 2432 54 1484 165 55
Fragments arranged by size:
2432 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2102 2102
Size: 4190
BsrFI r'CCGG_y
Cuts at: 2188 2369 2578 2188
Size: 181 209 3800
Fragments arranged by size:
3800 209 181
BsrGI T'GTAC_A
Cuts at: 690 690
Size: 4190
BssSI C'ACGA_G
Cuts at: 2461 2819 2461
Size: 358 3832
BstAPI GCAn_nnn'nTGC
Cuts at: 1567 1567
Size: 4190
BstBI TT'CG_AA
Cuts at: 276 714 2551 276
Size: 438 1837 1915
Fragments arranged by size:
1915 1837 438
BstYI r'GATC_y
Cuts at: 1 1215 1682 1705 2040 2286 3298 3384 3396
Size: 1214 467 23 335 246 1012 86 12
Cuts at: 3396 3742 1
Size: 346 449
Fragments arranged by size:
1214 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 962 962
Size: 4190
Bsu36I CC'TnA_GG
Cuts at: 1019 1019
Size: 4190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2140 2321 2484 2140
Size: 181 163 3846
Fragments arranged by size:
3846 181 163
BtsI GCAGTG_nn'
Cuts at: 1433 1433
Size: 4190
DraI TTT'AAA
Cuts at: 1028 1189 1028
Size: 161 4029
DraIII CAC_nnn'GTG
Cuts at: 438 438
Size: 4190
DrdI GACnn_nn'nnGTC
Cuts at: 1896 2754 1896
Size: 858 3332
EaeI y'GGCC_r
Cuts at: 1775 1949 2340 2367 3712 1775
Size: 174 391 27 1345 2253
Fragments arranged by size:
2253 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4190
EarI CTCTTCn'nnn_
Cuts at: 118 294 2213 2423 118
Size: 176 1919 210 1885
Fragments arranged by size:
1919 1885 210 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2398 2708 2854 4000 2398
Size: 310 146 1146 2588
Fragments arranged by size:
2588 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1150 1202 1150
Size: 52 4138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 2015 2447 3194 3462 515
Size: 1500 432 747 268 1243
Fragments arranged by size:
1500 1243 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1329 1329
Size: 4190
EcoRI G'AATT_C
Cuts at: 1114 1194 1114
Size: 80 4110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 4190
FspI TGC'GCA
Cuts at: 1971 1971
Size: 4190
HaeII r_GCGC'y
Cuts at: 453 1624 1872 2894 3873 453
Size: 1171 248 1022 979 770
Fragments arranged by size:
1171 1022 979 770 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 576 608 2032 2062 2064 2094 576
Size: 32 1424 30 2 30 2672
Fragments arranged by size:
2672 1424 32 30 30 2
HincII GTy'rAC
Cuts at: 1229 1229
Size: 4190
KasI G'GCGC_C
Cuts at: 449 1620 1868 449
Size: 1171 248 2771
Fragments arranged by size:
2771 1171 248
KpnI G_GTAC'C
Cuts at: 1210 1210
Size: 4190
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 298 522 2861 3045 298
Size: 224 2339 184 1443
Fragments arranged by size:
2339 1443 224 184
MscI TGG'CCA
Cuts at: 1951 1951
Size: 4190
MslI CAynn'nnrTG
Cuts at: 998 1055 2179 2306 998
Size: 57 1124 127 2882
Fragments arranged by size:
2882 1124 127 57
MspA1I CmG'CkG
Cuts at: 1301 1615 1975 2988 3233 1301
Size: 314 360 1013 245 2258
Fragments arranged by size:
2258 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2371 2371
Size: 4190
NarI GG'CG_CC
Cuts at: 450 1621 1869 450
Size: 1171 248 2771
Fragments arranged by size:
2771 1171 248
NgoMIV G'CCGG_C
Cuts at: 2369 2369
Size: 4190
NsiI A_TGCA'T
Cuts at: 834 834
Size: 4190
NspI r_CATG'y
Cuts at: 1243 1743 2650 1243
Size: 500 907 2783
Fragments arranged by size:
2783 907 500
PciI A'CATG_T
Cuts at: 2646 2646
Size: 4190
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PmlI CAC'GTG
Cuts at: 816 816
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 4158
PsiI TTA'TAA
Cuts at: 167 167
Size: 4190
PstI C_TGCA'G
Cuts at: 645 1237 1922 645
Size: 592 685 2913
Fragments arranged by size:
2913 685 592
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 1615 1975 1615
Size: 360 3830
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 1152 1204 1152
Size: 52 4138
SalI G'TCGA_C
Cuts at: 1227 1227
Size: 4190
SapI GCTCTTCn'nnn_
Cuts at: 294 2213 2423 294
Size: 1919 210 2061
Fragments arranged by size:
2061 1919 210
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4190
ScaI AGT'ACT
Cuts at: 3707 3707
Size: 4190
SfcI C'TryA_G
Cuts at: 33 641 1233 1918 2911 3102 33
Size: 608 592 685 993 191 1121
Fragments arranged by size:
1121 993 685 608 592 191
SfoI GGC'GCC
Cuts at: 451 1622 1870 451
Size: 1171 248 2771
Fragments arranged by size:
2771 1171 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SmaI CCC'GGG
Cuts at: 1212 1212
Size: 4190
SmlI C'TyrA_G
Cuts at: 841 1345 2260 2752 3014 841
Size: 504 915 492 262 2017
Fragments arranged by size:
2017 915 504 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3690
StyI C'CwwG_G
Cuts at: 1324 1678 1324
Size: 354 3836
SwaI ATTT'AAAT
Cuts at: 1028 1028
Size: 4190
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 233 1773 1906 2365 2574 233
Size: 1540 133 459 209 1849
Fragments arranged by size:
1849 1540 459 209 133
TatI w'GTAC_w
Cuts at: 690 3705 690
Size: 3015 1175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3707 3739 3707
Size: 32 4158
Tth111I GACn'n_nGTC
Cuts at: 769 1987 769
Size: 1218 2972
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1685 1685
Size: 4190
XmaI C'CCGG_G
Cuts at: 1210 1210
Size: 4190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PmeI
PmlI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI BpmI Bpu10I
BsiWI BssHII BstEII BstXI BtgI ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI XhoI XmnI ZraI