Restriction map for
pF1KB4447
(Circular) (Six-base) MAPSORT of: pF1KB4447.seq Check: 7346 from: 1 to: 4394
pF1KB4447 4394 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 143 668 143
Size: 525 3869
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 874 1410 874
Size: 536 3858
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 344 571 869 917 1146 2219 2651 3004
Size: 130 227 298 48 229 1073 432 353
Cuts at: 3004 3666 214
Size: 662 942
Fragments arranged by size:
1073 942 662 432 353 298 229 227
130 48
AflIII A'CryG_T
Cuts at: 801 1746 3546 3827 801
Size: 945 1800 281 1368
Fragments arranged by size:
1800 1368 945 281
AhdI GACnn_n'nnGTC
Cuts at: 314 314
Size: 4394
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 661 693 661
Size: 32 4362
AlwNI CAG_nnn'CTG
Cuts at: 1116 1234 1699 3137 1116
Size: 118 465 1438 2373
Fragments arranged by size:
2373 1438 465 118
ApaLI G'TGCA_C
Cuts at: 156 3232 156
Size: 3076 1318
ApoI r'AATT_y
Cuts at: 1398 3747 4003 1398
Size: 2349 256 1789
Fragments arranged by size:
2349 1789 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 126 689 1414 126
Size: 563 725 3106
Fragments arranged by size:
3106 725 563
BamHI G'GATC_C
Cuts at: 1419 1419
Size: 4394
BanI G'GyrC_C
Cuts at: 188 563 874 891 1410 1824 2072 2107 188
Size: 375 311 17 519 414 248 35 2475
Fragments arranged by size:
2475 519 414 375 311 248 35 17
BanII G_rGCy'C
Cuts at: 650 689 1123 1408 2438 650
Size: 39 434 285 1030 2606
Fragments arranged by size:
2606 1030 434 285 39
BbeI G_GCGC'C
Cuts at: 1828 2076 1828
Size: 248 4146
BbsI GAAGACnn'nnnn_
Cuts at: 857 857
Size: 4394
BbvCI CC'TCA_GC
Cuts at: 710 710
Size: 4394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4360
BciVI GTATCCnnnnn_n'
Cuts at: 1014 2292 3348 4394 1014
Size: 1278 1056 1046 1014
Fragments arranged by size:
1278 1056 1046 1014
BclI T'GATC_A
Cuts at: 273 1914 273
Size: 1641 2753
BglII A'GATC_T
Cuts at: 1909 3946 1909
Size: 2037 2357
BlpI GC'TnA_GC
Cuts at: 1506 1506
Size: 4394
Bme1580I G_kGCm'C
Cuts at: 160 2019 2112 3236 160
Size: 1859 93 1124 1318
Fragments arranged by size:
1859 1318 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1185 1606 1687 2022 4166 1185
Size: 421 81 335 2144 1413
Fragments arranged by size:
2144 1413 421 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1220 2761 1220
Size: 1541 2853
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 312 703 312
Size: 391 4003
Bpu10I CC'TnA_GC
Cuts at: 710 710
Size: 4394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 1317 1570 2449 3163 3461 1159
Size: 158 253 879 714 298 2092
Fragments arranged by size:
2092 879 714 298 253 158
BsaI GGTCTCn'nnnn_
Cuts at: 4098 4098
Size: 4394
BsaAI yAC'GTr
Cuts at: 804 1747 804
Size: 943 3451
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 1825 2073 2775 4271 1825
Size: 248 702 1496 1948
Fragments arranged by size:
1948 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1583 2104 2782 3193 3340 3614 1583
Size: 521 678 411 147 274 2363
Fragments arranged by size:
2363 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 661 691 724 754 661
Size: 30 33 30 4301
Fragments arranged by size:
4301 33 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 238
Size: 4394
BseYI C'CCAG_C
Cuts at: 300 304 377 1116 1819 2577 3242 3650 300
Size: 4 73 739 703 758 665 408 1044
Fragments arranged by size:
1044 758 739 703 665 408 73 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1012 721
Size: 291 4103
BsiEI CG_ry'CG
Cuts at: 104 1936 1982 3212 104
Size: 1832 46 1230 1286
Fragments arranged by size:
1832 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 160 626 650 1408 2186 2376 3236 160
Size: 466 24 758 778 190 860 1318
Fragments arranged by size:
1318 860 778 758 466 190 24
BsmI GAATG_Cn'
Cuts at: 1339 1339
Size: 4394
Bsp1286I G_dGCh'C
Cuts at: 160 626 650 689 1123 1408 2019 2112 2186
Size: 466 24 39 434 285 611 93 74
Cuts at: 2186 2376 2438 3236 160
Size: 190 62 798 1318
Fragments arranged by size:
1318 798 611 466 434 285 190 93
74 62 39 24
BspHI T'CATG_A
Cuts at: 2826 2826
Size: 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 143 668 743 1242 1444 1658 1960 2341 143
Size: 525 75 499 202 214 302 381 2196
Fragments arranged by size:
2196 525 499 381 302 214 202 75
BsrBI CCG'CTC
Cuts at: 50 2686 2740 4224 4389 50
Size: 2636 54 1484 165 55
Fragments arranged by size:
2636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 276 2306 276
Size: 2030 2364
BsrFI r'CCGG_y
Cuts at: 674 2392 2573 674
Size: 1718 181 2495
Fragments arranged by size:
2495 1718 181
BssSI C'ACGA_G
Cuts at: 574 2665 3373 574
Size: 2091 708 1595
Fragments arranged by size:
2091 1595 708
BstAPI GCAn_nnn'nTGC
Cuts at: 117 1771 117
Size: 1654 2740
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstYI r'GATC_y
Cuts at: 1 762 1043 1419 1886 1909 2244 2490 2796
Size: 761 281 376 467 23 335 246 306
Cuts at: 2796 2808 2894 3946 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 761 467 449 376 335 306 281
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 141 141
Size: 4394
BtgI C'CryG_G
Cuts at: 383 972 383
Size: 589 3805
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 190 479 546 935 1637 190
Size: 289 67 389 702 2947
Fragments arranged by size:
2947 702 389 289 67
DraI TTT'AAA
Cuts at: 1393 1393
Size: 4394
DraIII CAC_nnn'GTG
Cuts at: 807 807
Size: 4394
DrdI GACnn_nn'nnGTC
Cuts at: 2100 3444 2100
Size: 1344 3050
EaeI y'GGCC_r
Cuts at: 440 716 1064 1313 1979 2153 2544 2571 3916
Size: 276 348 249 666 174 391 27 1345
Cuts at: 3916 440
Size: 918
Fragments arranged by size:
1345 918 666 391 348 276 249 174
27
EagI C'GGCC_G
Cuts at: 1979 1979
Size: 4394
EarI CTCTTCn'nnn_
Cuts at: 655 2417 2627 655
Size: 1762 210 2422
Fragments arranged by size:
2422 1762 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 224 2602 3344 3490 4204 224
Size: 2378 742 146 714 414
Fragments arranged by size:
2378 742 714 414 146
EcoICRI GAG'CTC
Cuts at: 648 1406 648
Size: 758 3636
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 312 344 571 703 869 917 1146 2219
Size: 98 32 227 132 166 48 229 1073
Cuts at: 2219 2651 3004 3666 214
Size: 432 353 662 942
Fragments arranged by size:
1073 942 662 432 353 229 227 166
132 98 48 32
EcoNI CCTnn'n_nnAGG
Cuts at: 129 829 129
Size: 700 3694
EcoO109I rG'GnC_Cy
Cuts at: 308 355 432 1231 1328 1533 308
Size: 47 77 799 97 205 3169
Fragments arranged by size:
3169 799 205 97 77 47
EcoRI G'AATT_C
Cuts at: 1398 1398
Size: 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 516 633 2413 516
Size: 117 1780 2497
Fragments arranged by size:
2497 1780 117
FspI TGC'GCA
Cuts at: 2175 2175
Size: 4394
HaeII r_GCGC'y
Cuts at: 675 725 1828 2076 3306 4077 675
Size: 50 1103 248 1230 771 992
Fragments arranged by size:
1230 1103 992 771 248 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 300 332 844 876 2236 2266 2268 2298 300
Size: 32 512 32 1360 30 2 30 2396
Fragments arranged by size:
2396 1360 512 32 32 30 30 2
HincII GTy'rAC
Cuts at: 636 1433 636
Size: 797 3597
KasI G'GCGC_C
Cuts at: 1824 2072 1824
Size: 248 4146
KpnI G_GTAC'C
Cuts at: 878 1414 878
Size: 536 3858
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3153 3337 3153
Size: 184 4210
MscI TGG'CCA
Cuts at: 442 718 1315 2155 442
Size: 276 597 840 2681
Fragments arranged by size:
2681 840 597 276
MslI CAynn'nnrTG
Cuts at: 2383 2510 2383
Size: 127 4267
MspA1I CmG'CkG
Cuts at: 304 666 1113 1505 1819 2179 2963 3208 304
Size: 362 447 392 314 360 784 245 1490
Fragments arranged by size:
1490 784 447 392 362 360 314 245
NaeI GCC'GGC
Cuts at: 2575 2575
Size: 4394
NarI GG'CG_CC
Cuts at: 1825 2073 1825
Size: 248 4146
NcoI C'CATG_G
Cuts at: 972 972
Size: 4394
NgoMIV G'CCGG_C
Cuts at: 2573 2573
Size: 4394
NspI r_CATG'y
Cuts at: 1447 1947 3550 1447
Size: 500 1603 2291
Fragments arranged by size:
2291 1603 500
PciI A'CATG_T
Cuts at: 3546 3546
Size: 4394
PflMI CCAn_nnn'nTGG
Cuts at: 528 1175 528
Size: 647 3747
PfoI T'CCnGG_A
Cuts at: 680 680
Size: 4394
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PmlI CAC'GTG
Cuts at: 804 804
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 661 693 2840 2872 661
Size: 32 2147 32 2183
Fragments arranged by size:
2183 2147 32 32
PpuMI rG'GwC_Cy
Cuts at: 308 432 1231 308
Size: 124 799 3471
Fragments arranged by size:
3471 799 124
PstI C_TGCA'G
Cuts at: 665 1441 2126 665
Size: 776 685 2933
Fragments arranged by size:
2933 776 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 304 1819 2179 304
Size: 1515 360 2519
Fragments arranged by size:
2519 1515 360
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 650 1408 650
Size: 758 3636
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SanDI GG'GwC_CC
Cuts at: 308 308
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 655 2417 2627 655
Size: 1762 210 2422
Fragments arranged by size:
2422 1762 210
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 886 3911 886
Size: 3025 1369
SfcI C'TryA_G
Cuts at: 33 282 484 661 990 1039 1437 2122 3090
Size: 249 202 177 329 49 398 685 968
Cuts at: 3090 3281 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 398 329 249 202 191
177 49
SfoI GGC'GCC
Cuts at: 1826 2074 1826
Size: 248 4146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 691 1416 691
Size: 725 3669
SmlI C'TyrA_G
Cuts at: 1138 1332 1549 2464 3178 3440 1138
Size: 194 217 915 714 262 2092
Fragments arranged by size:
2092 915 714 262 217 194
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
StyI C'CwwG_G
Cuts at: 972 1528 1882 972
Size: 556 354 3484
Fragments arranged by size:
3484 556 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1282 1977 2110 2569 2778 1282
Size: 695 133 459 209 2898
Fragments arranged by size:
2898 695 459 209 133
TatI w'GTAC_w
Cuts at: 884 3909 884
Size: 3025 1369
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 791 823 925 957 3911 3943 791
Size: 32 102 32 2954 32 1242
Fragments arranged by size:
2954 1242 102 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 631 2191 631
Size: 1560 2834
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1889 1889
Size: 4394
XmaI C'CCGG_G
Cuts at: 689 1414 689
Size: 725 3669
XmnI GAAnn'nnTTC
Cuts at: 902 902
Size: 4394
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AlfI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsiWI BsmBI
BspEI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI