Restriction map for
pF1KB4431
(Circular) (Six-base) MAPSORT of: pF1KB4431.seq Check: 9009 from: 1 to: 5783
pF1KB4431 5783 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 2233 2821 2233
Size: 588 5195
Acc65I G'GTAC_C
Cuts at: 1395 2799 1395
Size: 1404 4379
AclI AA'CG_TT
Cuts at: 5623 5623
Size: 5783
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 439 1738 3608 4040 4393 5055 256
Size: 183 1299 1870 432 353 662 984
Fragments arranged by size:
1870 1299 984 662 432 353 183
AfeI AGC'GCT
Cuts at: 433 1080 433
Size: 647 5136
AflIII A'CryG_T
Cuts at: 1284 1842 2249 2287 2387 3135 4935 5216 1284
Size: 558 407 38 100 748 1800 281 1851
Fragments arranged by size:
1851 1800 748 558 407 281 100 38
AhdI GACnn_n'nnGTC
Cuts at: 1061 1061
Size: 5783
AlwNI CAG_nnn'CTG
Cuts at: 760 853 1332 2001 2645 2774 3088 4526 760
Size: 93 479 669 644 129 314 1438 2017
Fragments arranged by size:
2017 1438 669 644 479 314 129 93
ApaI G_GGCC'C
Cuts at: 1025 1372 1025
Size: 347 5436
ApaLI G'TGCA_C
Cuts at: 4621 4621
Size: 5783
ApoI r'AATT_y
Cuts at: 2787 5136 5392 2787
Size: 2349 256 3178
Fragments arranged by size:
3178 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5783
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5783
AvaI C'yCGr_G
Cuts at: 175 1147 1514 1570 2436 2803 175
Size: 972 367 56 866 367 3155
Fragments arranged by size:
3155 972 866 367 367 56
BamHI G'GATC_C
Cuts at: 1260 2808 1260
Size: 1548 4235
BanI G'GyrC_C
Cuts at: 455 972 1044 1395 2799 3213 3461 3496 455
Size: 517 72 351 1404 414 248 35 2742
Fragments arranged by size:
2742 1404 517 414 351 248 72 35
BanII G_rGCy'C
Cuts at: 143 252 867 1025 1372 2050 2546 2797 3827
Size: 109 615 158 347 678 496 251 1030
Cuts at: 3827 143
Size: 2099
Fragments arranged by size:
2099 1030 678 615 496 347 251 158
109
BbeI G_GCGC'C
Cuts at: 3217 3465 3217
Size: 248 5535
BbvCI CC'TCA_GC
Cuts at: 115 412 2537 115
Size: 297 2125 3361
Fragments arranged by size:
3361 2125 297
BciVI GTATCCnnnnn_n'
Cuts at: 3681 4737 5783 3681
Size: 1056 1046 3681
Fragments arranged by size:
3681 1056 1046
BclI T'GATC_A
Cuts at: 3303 3303
Size: 5783
BglI GCCn_nnn'nGGC
Cuts at: 171 501 1367 171
Size: 330 866 4587
Fragments arranged by size:
4587 866 330
BglII A'GATC_T
Cuts at: 485 546 2144 3298 5335 485
Size: 61 1598 1154 2037 933
Fragments arranged by size:
2037 1598 1154 933 61
BlpI GC'TnA_GC
Cuts at: 2678 2895 2678
Size: 217 5566
Bme1580I G_kGCm'C
Cuts at: 944 1025 1049 1372 3408 3501 4625 944
Size: 81 24 323 2036 93 1124 2102
Fragments arranged by size:
2102 2036 1124 323 93 81 24
BmgBI CAC'GTC
Cuts at: 2250 2388 2250
Size: 138 5645
BmrI ACTGGGnnnn_n'
Cuts at: 376 1050 2633 2995 3076 3411 5555 376
Size: 674 1583 362 81 335 2144 604
Fragments arranged by size:
2144 1583 674 604 362 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1749 4150 1749
Size: 2401 3382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1341 1651 1978 2230 2433 2485 2584 1341
Size: 310 327 252 203 52 99 4540
Fragments arranged by size:
4540 327 310 252 203 99 52
Bpu10I CC'TnA_GC
Cuts at: 115 412 2044 2537 115
Size: 297 1632 493 3361
Fragments arranged by size:
3361 1632 493 297
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1767 2959 3838 4552 4850 1767
Size: 1192 879 714 298 2700
Fragments arranged by size:
2700 1192 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1344 2063 5487 1344
Size: 719 3424 1640
Fragments arranged by size:
3424 1640 719
BsaAI yAC'GTr
Cuts at: 1285 1845 2290 3136 1285
Size: 560 445 846 3932
Fragments arranged by size:
3932 846 560 445
BsaBI GATnn'nnATC
Cuts at: 6 3297 5334 6
Size: 3291 2037 455
Fragments arranged by size:
3291 2037 455
BsaHI Gr'CG_yC
Cuts at: 440 2299 3214 3462 4164 5660 440
Size: 1859 915 248 702 1496 563
Fragments arranged by size:
1859 1496 915 702 563 248
BsaWI w'CCGG_w
Cuts at: 1871 2006 2370 2972 3493 4171 4582 4729 5003
Size: 135 364 602 521 678 411 147 274
Cuts at: 5003 1871
Size: 2651
Fragments arranged by size:
2651 678 602 521 411 364 274 147
135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 239 269 2374 2404 239
Size: 30 2105 30 3618
Fragments arranged by size:
3618 2105 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 331 1364 2554 331
Size: 1033 1190 3560
Fragments arranged by size:
3560 1190 1033
BseYI C'CCAG_C
Cuts at: 491 911 1071 1256 1712 2599 3208 3966 4631
Size: 420 160 185 456 887 609 758 665
Cuts at: 4631 5039 491
Size: 408 1235
Fragments arranged by size:
1235 887 758 665 609 456 420 408
185 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 2260 1376
Size: 884 4899
BsiEI CG_ry'CG
Cuts at: 104 136 447 3325 3371 4601 104
Size: 32 311 2878 46 1230 1286
Fragments arranged by size:
2878 1286 1230 311 46 32
BsiHKAI G_wGCw'C
Cuts at: 703 867 1643 2050 2702 2797 3575 3765 4625
Size: 164 776 407 652 95 778 190 860
Cuts at: 4625 703
Size: 1861
Fragments arranged by size:
1861 860 778 776 652 407 190 164
95
BsiWI C'GTAC_G
Cuts at: 1281 1281
Size: 5783
BsmBI CGTCTCn'nnnn_
Cuts at: 1299 1461 1471 1897 1299
Size: 162 10 426 5185
Fragments arranged by size:
5185 426 162 10
Bsp1286I G_dGCh'C
Cuts at: 143 252 703 867 944 1025 1049 1372 1643
Size: 109 451 164 77 81 24 323 271
Cuts at: 1643 2050 2546 2702 2797 3408 3501 3575 3765
Size: 407 496 156 95 611 93 74 190
Cuts at: 3765 3827 4625 143
Size: 62 798 1301
Fragments arranged by size:
1301 798 611 496 451 407 323 271
190 164 156 109 95 93 81 77
74 62 24
BspEI T'CCGG_A
Cuts at: 1871 2370 1871
Size: 499 5284
BspHI T'CATG_A
Cuts at: 1347 1922 2282 4215 1347
Size: 575 360 1933 2915
Fragments arranged by size:
2915 1933 575 360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 220 961 1946 2833 3047 3349 3730 220
Size: 741 985 887 214 302 381 2273
Fragments arranged by size:
2273 985 887 741 381 302 214
BsrBI CCG'CTC
Cuts at: 50 658 4075 4129 5613 5778 50
Size: 608 3417 54 1484 165 55
Fragments arranged by size:
3417 1484 608 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1775 2021 3695 1775
Size: 246 1674 3863
Fragments arranged by size:
3863 1674 246
BsrFI r'CCGG_y
Cuts at: 282 442 446 3781 3962 282
Size: 160 4 3335 181 2103
Fragments arranged by size:
3335 2103 181 160 4
BsrGI T'GTAC_A
Cuts at: 374 880 374
Size: 506 5277
BssSI C'ACGA_G
Cuts at: 4054 4762 4054
Size: 708 5075
BstAPI GCAn_nnn'nTGC
Cuts at: 781 3160 781
Size: 2379 3404
BstBI TT'CG_AA
Cuts at: 1461 4144 1461
Size: 2683 3100
BstEII G'GTnAC_C
Cuts at: 690 2265 690
Size: 1575 4208
BstXI CCAn_nnnn'nTGG
Cuts at: 911 1017 1905 911
Size: 106 888 4789
Fragments arranged by size:
4789 888 106
BstYI r'GATC_y
Cuts at: 1 485 546 1260 2144 2808 3275 3298 3633
Size: 484 61 714 884 664 467 23 335
Cuts at: 3633 3879 4185 4197 4283 5335 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 884 714 664 484 467 449 335
306 246 86 61 23 12
BstZ17I GTA'TAC
Cuts at: 2234 2234
Size: 5783
Bsu36I CC'TnA_GG
Cuts at: 994 1804 994
Size: 810 4973
BtgI C'CryG_G
Cuts at: 107 169 1904 107
Size: 62 1735 3986
Fragments arranged by size:
3986 1735 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1625 3733 3914 4077 1625
Size: 2108 181 163 3331
Fragments arranged by size:
3331 2108 181 163
BtsI GCAGTG_nn'
Cuts at: 306 560 775 2546 2616 2630 3026 306
Size: 254 215 1771 70 14 396 3063
Fragments arranged by size:
3063 1771 396 254 215 70 14
DraI TTT'AAA
Cuts at: 677 2782 677
Size: 2105 3678
DrdI GACnn_nn'nnGTC
Cuts at: 1322 3489 4833 1322
Size: 2167 1344 2272
Fragments arranged by size:
2272 2167 1344
EaeI y'GGCC_r
Cuts at: 133 444 858 1847 2219 3368 3542 3933 3960
Size: 311 414 989 372 1149 174 391 27
Cuts at: 3960 5305 133
Size: 1345 611
Fragments arranged by size:
1345 1149 989 611 414 391 372 311
174 27
EagI C'GGCC_G
Cuts at: 133 444 3368 133
Size: 311 2924 2548
Fragments arranged by size:
2924 2548 311
EarI CTCTTCn'nnn_
Cuts at: 750 1347 1790 3806 4016 750
Size: 597 443 2016 210 2517
Fragments arranged by size:
2517 2016 597 443 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 135 210 3991 4733 4879 5593 135
Size: 75 3781 742 146 714 325
Fragments arranged by size:
3781 742 714 325 146 75
EcoICRI GAG'CTC
Cuts at: 865 2048 2795 865
Size: 1183 747 3853
Fragments arranged by size:
3853 1183 747
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 439 1341 1651 1738 1978 2230 2433 2485
Size: 183 902 310 87 240 252 203 52
Cuts at: 2485 2584 3608 4040 4393 5055 256
Size: 99 1024 432 353 662 984
Fragments arranged by size:
1024 984 902 662 432 353 310 252
240 203 183 99 87 52
EcoNI CCTnn'n_nnAGG
Cuts at: 1160 1160
Size: 5783
EcoO109I rG'GnC_Cy
Cuts at: 598 652 900 966 1021 1022 1152 1165 1174
Size: 54 248 66 55 1 130 13 9
Cuts at: 1174 1272 1368 1369 1935 2508 2922 598
Size: 98 96 1 566 573 414 3459
Fragments arranged by size:
3459 573 566 414 248 130 98 96
66 55 54 13 9 1 1
EcoRI G'AATT_C
Cuts at: 2787 2787
Size: 5783
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3802 3802
Size: 5783
FspI TGC'GCA
Cuts at: 3564 3564
Size: 5783
HaeII r_GCGC'y
Cuts at: 115 435 1082 2436 3217 3465 4695 5466 115
Size: 320 647 1354 781 248 1230 771 432
Fragments arranged by size:
1354 1230 781 771 647 432 320 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 255 287 1047 1079 3625 3655 3657 3687 255
Size: 32 760 32 2546 30 2 30 2351
Fragments arranged by size:
2546 2351 760 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2822 2822
Size: 5783
HindIII A'AGCT_T
Cuts at: 811 811
Size: 5783
KasI G'GCGC_C
Cuts at: 3213 3461 3213
Size: 248 5535
KpnI G_GTAC'C
Cuts at: 1399 2803 1399
Size: 1404 4379
MluI A'CGCG_T
Cuts at: 5216 5216
Size: 5783
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 600 4542 4726 600
Size: 3942 184 1657
Fragments arranged by size:
3942 1657 184
MscI TGG'CCA
Cuts at: 1849 3544 1849
Size: 1695 4088
MslI CAynn'nnrTG
Cuts at: 802 1352 1903 2110 3772 3899 802
Size: 550 551 207 1662 127 2686
Fragments arranged by size:
2686 1662 551 550 207 127
MspA1I CmG'CkG
Cuts at: 150 156 171 1716 2484 2894 3208 3568 4352
Size: 6 15 1545 768 410 314 360 784
Cuts at: 4352 4597 150
Size: 245 1336
Fragments arranged by size:
1545 1336 784 768 410 360 314 245
15 6
NaeI GCC'GGC
Cuts at: 444 3964 444
Size: 3520 2263
NarI GG'CG_CC
Cuts at: 3214 3462 3214
Size: 248 5535
NcoI C'CATG_G
Cuts at: 107 1904 107
Size: 1797 3986
NdeI CA'TA_TG
Cuts at: 2304 2304
Size: 5783
NgoMIV G'CCGG_C
Cuts at: 442 3962 442
Size: 3520 2263
NspI r_CATG'y
Cuts at: 1945 2094 2836 3336 4939 1945
Size: 149 742 500 1603 2789
Fragments arranged by size:
2789 1603 742 500 149
PasI CC'CwG_GG
Cuts at: 1170 1665 1170
Size: 495 5288
PciI A'CATG_T
Cuts at: 4935 4935
Size: 5783
PflMI CCAn_nnn'nTGG
Cuts at: 363 623 2308 363
Size: 260 1685 3838
Fragments arranged by size:
3838 1685 260
PfoI T'CCnGG_A
Cuts at: 212 962 1960 2137 212
Size: 750 998 177 3858
Fragments arranged by size:
3858 998 750 177
PmeI GTTT'AAAC
Cuts at: 2782 2782
Size: 5783
PmlI CAC'GTG
Cuts at: 1845 2290 1845
Size: 445 5338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1642 1674 4229 4261 1642
Size: 32 2555 32 3164
Fragments arranged by size:
3164 2555 32 32
PpuMI rG'GwC_Cy
Cuts at: 598 652 966 1174 1272 1935 2508 598
Size: 54 314 208 98 663 573 3873
Fragments arranged by size:
3873 663 573 314 208 98 54
PspOMI G'GGCC_C
Cuts at: 1021 1368 1021
Size: 347 5436
PstI C_TGCA'G
Cuts at: 301 786 2750 2830 3515 301
Size: 485 1964 80 685 2569
Fragments arranged by size:
2569 1964 685 485 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5783
PvuII CAG'CTG
Cuts at: 1716 2484 3208 3568 1716
Size: 768 724 360 3931
Fragments arranged by size:
3931 768 724 360
RsrII CG'GwC_CG
Cuts at: 3978 3978
Size: 5783
SacI G_AGCT'C
Cuts at: 867 2050 2797 867
Size: 1183 747 3853
Fragments arranged by size:
3853 1183 747
SacII CC_GC'GG
Cuts at: 172 172
Size: 5783
SalI G'TCGA_C
Cuts at: 2820 2820
Size: 5783
SanDI GG'GwC_CC
Cuts at: 598 652 1174 598
Size: 54 522 5207
Fragments arranged by size:
5207 522 54
SapI GCTCTTCn'nnn_
Cuts at: 3806 4016 3806
Size: 210 5573
SbfI CC_TGCA'GG
Cuts at: 2830 2830
Size: 5783
ScaI AGT'ACT
Cuts at: 5300 5300
Size: 5783
SexAI A'CCwGG_T
Cuts at: 968 968
Size: 5783
SfcI C'TryA_G
Cuts at: 33 297 782 871 987 2148 2589 2746 2826
Size: 264 485 89 116 1161 441 157 80
Cuts at: 2826 3511 4479 4670 33
Size: 685 968 191 1146
Fragments arranged by size:
1161 1146 968 685 485 441 264 191
157 116 89 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 1367 1367
Size: 5783
SfoI GGC'GCC
Cuts at: 3215 3463 3215
Size: 248 5535
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5783
SmaI CCC'GGG
Cuts at: 1516 2805 1516
Size: 1289 4494
SmlI C'TyrA_G
Cuts at: 1782 2938 3853 4567 4829 1782
Size: 1156 915 714 262 2736
Fragments arranged by size:
2736 1156 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5783
SphI G_CATG'C
Cuts at: 1945 2094 2836 3336 1945
Size: 149 742 500 4392
Fragments arranged by size:
4392 742 500 149
StuI AGG'CCT
Cuts at: 737 1118 737
Size: 381 5402
StyI C'CwwG_G
Cuts at: 107 1016 1058 1407 1904 2917 3271 107
Size: 909 42 349 497 1013 354 2619
Fragments arranged by size:
2619 1013 909 497 354 349 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 324 576 1032 2617 3366 3499 3958 4167 324
Size: 252 456 1585 749 133 459 209 1940
Fragments arranged by size:
1940 1585 749 459 456 252 209 133
TatI w'GTAC_w
Cuts at: 374 880 5298 374
Size: 506 4418 859
Fragments arranged by size:
4418 859 506
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 239 271 834 866 5300 5332 239
Size: 32 563 32 4434 32 690
Fragments arranged by size:
4434 690 563 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 267 1504 3580 267
Size: 1237 2076 2470
Fragments arranged by size:
2470 2076 1237
XbaI T'CTAG_A
Cuts at: 2814 2814
Size: 5783
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 466 3278 466
Size: 2812 2971
XmaI C'CCGG_G
Cuts at: 1514 2803 1514
Size: 1289 4494
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BmtI BsmI BssHII
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI NsiI PacI PshAI PsiI PspXI
PsrI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI