Restriction map for
pF1KB4417
(Circular) (Six-base) MAPSORT of: pF1KB4417.seq Check: 3317 from: 1 to: 4583
pF1KB4417 4583 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 1621 1621
Size: 4583
Acc65I G'GTAC_C
Cuts at: 1599 1599
Size: 4583
AclI AA'CG_TT
Cuts at: 4423 4423
Size: 4583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 1051 2408 2840 3587 3855 157
Size: 894 1357 432 747 268 885
Fragments arranged by size:
1357 894 885 747 432 268
AflIII A'CryG_T
Cuts at: 1935 3039 4016 1935
Size: 1104 977 2502
Fragments arranged by size:
2502 1104 977
AgeI A'CCGG_T
Cuts at: 2971 2971
Size: 4583
AleI CACnn'nnGTG
Cuts at: 1002 1002
Size: 4583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 674 1459 674
Size: 785 3798
AlwNI CAG_nnn'CTG
Cuts at: 135 567 645 690 1130 1205 1888 3455 135
Size: 432 78 45 440 75 683 1567 1263
Fragments arranged by size:
1567 1263 683 440 432 78 75 45
ApaI G_GGCC'C
Cuts at: 317 620 317
Size: 303 4280
ApaLI G'TGCA_C
Cuts at: 3353 3353
Size: 4583
ApoI r'AATT_y
Cuts at: 1587 3936 4192 1587
Size: 2349 256 1978
Fragments arranged by size:
2349 1978 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4583
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
AvaI C'yCGr_G
Cuts at: 830 1603 830
Size: 773 3810
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 109 142 109
Size: 33 4550
BamHI G'GATC_C
Cuts at: 1608 1608
Size: 4583
BanI G'GyrC_C
Cuts at: 141 570 658 1435 1599 2013 2261 2296 141
Size: 429 88 777 164 414 248 35 2428
Fragments arranged by size:
2428 777 429 414 248 164 88 35
BanII G_rGCy'C
Cuts at: 158 264 317 524 620 1597 2627 158
Size: 106 53 207 96 977 1030 2114
Fragments arranged by size:
2114 1030 977 207 106 96 53
BbeI G_GCGC'C
Cuts at: 2017 2265 2017
Size: 248 4335
BbvCI CC'TCA_GC
Cuts at: 1542 1542
Size: 4583
BciVI GTATCCnnnnn_n'
Cuts at: 2481 3242 4583 2481
Size: 761 1341 2481
Fragments arranged by size:
2481 1341 761
BclI T'GATC_A
Cuts at: 2103 2103
Size: 4583
BglI GCCn_nnn'nGGC
Cuts at: 615 878 982 615
Size: 263 104 4216
Fragments arranged by size:
4216 263 104
BglII A'GATC_T
Cuts at: 845 947 2098 4135 845
Size: 102 1151 2037 1293
Fragments arranged by size:
2037 1293 1151 102
BlpI GC'TnA_GC
Cuts at: 1695 1695
Size: 4583
Bme1580I G_kGCm'C
Cuts at: 317 620 663 1438 2208 2301 3357 317
Size: 303 43 775 770 93 1056 1543
Fragments arranged by size:
1543 1056 775 770 303 93 43
BmrI ACTGGGnnnn_n'
Cuts at: 1518 1795 1876 2211 4355 1518
Size: 277 81 335 2144 1746
Fragments arranged by size:
2144 1746 335 277 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2950 2950
Size: 4583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1007 1126 1507 1007
Size: 119 381 4083
Fragments arranged by size:
4083 381 119
Bpu10I CC'TnA_GC
Cuts at: 1542 1542
Size: 4583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1759 2638 3130 3428 1759
Size: 879 492 298 2914
Fragments arranged by size:
2914 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 827 4287 827
Size: 3460 1123
BsaAI yAC'GTr
Cuts at: 1936 1936
Size: 4583
BsaBI GATnn'nnATC
Cuts at: 6 2097 4134 6
Size: 2091 2037 455
Fragments arranged by size:
2091 2037 455
BsaHI Gr'CG_yC
Cuts at: 701 1178 2014 2262 2964 4460 701
Size: 477 836 248 702 1496 824
Fragments arranged by size:
1496 836 824 702 477 248
BsaWI w'CCGG_w
Cuts at: 1772 2293 2971 3245 3392 3803 1772
Size: 521 678 274 147 411 2552
Fragments arranged by size:
2552 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 460 490 1498 1528 460
Size: 30 1008 30 3515
Fragments arranged by size:
3515 1008 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 556 166
Size: 390 4193
BseYI C'CCAG_C
Cuts at: 218 327 435 530 610 620 795 1205 2008
Size: 109 108 95 80 10 175 410 803
Cuts at: 2008 2766 3343 3839 218
Size: 758 577 496 962
Fragments arranged by size:
962 803 758 577 496 410 175 109
108 95 80 10
BsiEI CG_ry'CG
Cuts at: 104 2125 2171 3379 104
Size: 2021 46 1208 1308
Fragments arranged by size:
2021 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1597 2375 2565 3357 1597
Size: 778 190 792 2823
Fragments arranged by size:
2823 792 778 190
BsmI GAATG_Cn'
Cuts at: 371 371
Size: 4583
BsmBI CGTCTCn'nnnn_
Cuts at: 1420 1420
Size: 4583
Bsp1286I G_dGCh'C
Cuts at: 158 264 317 524 620 663 1438 1597 2208
Size: 106 53 207 96 43 775 159 611
Cuts at: 2208 2301 2375 2565 2627 3357 158
Size: 93 74 190 62 730 1384
Fragments arranged by size:
1384 775 730 611 207 190 159 106
96 93 74 62 53 43
BspEI T'CCGG_A
Cuts at: 3803 3803
Size: 4583
BspHI T'CATG_A
Cuts at: 1085 3759 1085
Size: 2674 1909
BspMI ACCTGCnnnn'nnnn_
Cuts at: 644 1103 1633 1847 2149 2530 644
Size: 459 530 214 302 381 2697
Fragments arranged by size:
2697 530 459 381 302 214
BsrBI CCG'CTC
Cuts at: 50 656 2875 2929 4413 4578 50
Size: 606 2219 54 1484 165 55
Fragments arranged by size:
2219 1484 606 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2495 2495
Size: 4583
BsrFI r'CCGG_y
Cuts at: 448 2581 2762 2971 448
Size: 2133 181 209 2060
Fragments arranged by size:
2133 2060 209 181
BsrGI T'GTAC_A
Cuts at: 809 809
Size: 4583
BssSI C'ACGA_G
Cuts at: 2854 3212 2854
Size: 358 4225
BstAPI GCAn_nnn'nTGC
Cuts at: 645 1565 1960 645
Size: 920 395 3268
Fragments arranged by size:
3268 920 395
BstBI TT'CG_AA
Cuts at: 2944 2944
Size: 4583
BstXI CCAn_nnnn'nTGG
Cuts at: 899 899
Size: 4583
BstYI r'GATC_y
Cuts at: 1 845 947 1608 2075 2098 2433 2679 3691
Size: 844 102 661 467 23 335 246 1012
Cuts at: 3691 3777 3789 4135 1
Size: 86 12 346 449
Fragments arranged by size:
1012 844 661 467 449 346 335 246
102 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 377 1501 377
Size: 1124 3459
BtgI C'CryG_G
Cuts at: 404 696 1310 404
Size: 292 614 3677
Fragments arranged by size:
3677 614 292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 456 579 2533 2714 2877 456
Size: 123 1954 181 163 2162
Fragments arranged by size:
2162 1954 181 163 123
BtsI GCAGTG_nn'
Cuts at: 362 759 1826 362
Size: 397 1067 3119
Fragments arranged by size:
3119 1067 397
DraI TTT'AAA
Cuts at: 1582 1582
Size: 4583
DrdI GACnn_nn'nnGTC
Cuts at: 2289 3147 2289
Size: 858 3725
EaeI y'GGCC_r
Cuts at: 1014 2168 2342 2733 2760 4105 1014
Size: 1154 174 391 27 1345 1492
Fragments arranged by size:
1492 1345 1154 391 174 27
EagI C'GGCC_G
Cuts at: 2168 2168
Size: 4583
EarI CTCTTCn'nnn_
Cuts at: 2606 2816 2606
Size: 210 4373
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 646 2791 3101 3247 4393 646
Size: 2145 310 146 1146 836
Fragments arranged by size:
2145 1146 836 310 146
EcoICRI GAG'CTC
Cuts at: 1595 1595
Size: 4583
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 1007 1051 1126 1507 2408 2840 3587 3855
Size: 850 44 75 381 901 432 747 268
Cuts at: 3855 157
Size: 885
Fragments arranged by size:
901 885 850 747 432 381 268 75
44
EcoNI CCTnn'n_nnAGG
Cuts at: 871 871
Size: 4583
EcoO109I rG'GnC_Cy
Cuts at: 222 381 546 617 1722 222
Size: 159 165 71 1105 3083
Fragments arranged by size:
3083 1105 165 159 71
EcoRI G'AATT_C
Cuts at: 1587 1587
Size: 4583
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 4583
FspI TGC'GCA
Cuts at: 455 2364 455
Size: 1909 2674
FspAI rTGC'GCAy
Cuts at: 455 455
Size: 4583
HaeII r_GCGC'y
Cuts at: 327 2017 2265 3287 4266 327
Size: 1690 248 1022 979 644
Fragments arranged by size:
1690 1022 979 644 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 460 492 1498 1530 2425 2455 2457 2487 460
Size: 32 1006 32 895 30 2 30 2556
Fragments arranged by size:
2556 1006 895 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1350 1622 1350
Size: 272 4311
KasI G'GCGC_C
Cuts at: 2013 2261 2013
Size: 248 4335
KpnI G_GTAC'C
Cuts at: 1603 1603
Size: 4583
MluI A'CGCG_T
Cuts at: 4016 4016
Size: 4583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1424 3254 3438 1424
Size: 1830 184 2569
Fragments arranged by size:
2569 1830 184
MscI TGG'CCA
Cuts at: 2344 2344
Size: 4583
MslI CAynn'nnrTG
Cuts at: 598 1002 1288 2572 2699 598
Size: 404 286 1284 127 2482
Fragments arranged by size:
2482 1284 404 286 127
MspA1I CmG'CkG
Cuts at: 218 276 558 614 821 1202 1312 1485 1546
Size: 58 282 56 207 381 110 173 61
Cuts at: 1546 1694 2008 2368 3381 3626 218
Size: 148 314 360 1013 245 1175
Fragments arranged by size:
1175 1013 381 360 314 282 245 207
173 148 110 61 58 56
NaeI GCC'GGC
Cuts at: 2764 2764
Size: 4583
NarI GG'CG_CC
Cuts at: 2014 2262 2014
Size: 248 4335
NcoI C'CATG_G
Cuts at: 696 696
Size: 4583
NdeI CA'TA_TG
Cuts at: 1338 1338
Size: 4583
NgoMIV G'CCGG_C
Cuts at: 2762 2762
Size: 4583
NspI r_CATG'y
Cuts at: 684 1636 2136 3043 684
Size: 952 500 907 2224
Fragments arranged by size:
2224 952 907 500
PciI A'CATG_T
Cuts at: 3039 3039
Size: 4583
PflMI CCAn_nnn'nTGG
Cuts at: 719 719
Size: 4583
PmeI GTTT'AAAC
Cuts at: 1582 1582
Size: 4583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 954 986 3722 3754 954
Size: 32 2736 32 1783
Fragments arranged by size:
2736 1783 32 32
PpuMI rG'GwC_Cy
Cuts at: 222 546 222
Size: 324 4259
PshAI GACnn'nnGTC
Cuts at: 784 784
Size: 4583
PspOMI G'GGCC_C
Cuts at: 313 616 313
Size: 303 4280
PstI C_TGCA'G
Cuts at: 150 563 641 653 820 1630 2315 150
Size: 413 78 12 167 810 685 2418
Fragments arranged by size:
2418 810 685 413 167 78 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4583
PvuII CAG'CTG
Cuts at: 276 558 821 1202 1485 1546 2008 2368 276
Size: 282 263 381 283 61 462 360 2491
Fragments arranged by size:
2491 462 381 360 283 282 263 61
RsrII CG'GwC_CG
Cuts at: 2778 2778
Size: 4583
SacI G_AGCT'C
Cuts at: 1597 1597
Size: 4583
SacII CC_GC'GG
Cuts at: 1313 1313
Size: 4583
SalI G'TCGA_C
Cuts at: 1620 1620
Size: 4583
SapI GCTCTTCn'nnn_
Cuts at: 2606 2816 2606
Size: 210 4373
SbfI CC_TGCA'GG
Cuts at: 641 1630 641
Size: 989 3594
ScaI AGT'ACT
Cuts at: 4100 4100
Size: 4583
SfcI C'TryA_G
Cuts at: 33 146 559 637 649 816 1626 2311 3304
Size: 113 413 78 12 167 810 685 993
Cuts at: 3304 3495 33
Size: 191 1121
Fragments arranged by size:
1121 993 810 685 413 191 167 113
78 12
SfoI GGC'GCC
Cuts at: 2015 2263 2015
Size: 248 4335
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
SgrAI Cr'CCGG_yG
Cuts at: 448 448
Size: 4583
SmaI CCC'GGG
Cuts at: 1605 1605
Size: 4583
SmlI C'TyrA_G
Cuts at: 830 1738 2653 3145 3407 830
Size: 908 915 492 262 2006
Fragments arranged by size:
2006 915 908 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4583
SphI G_CATG'C
Cuts at: 1636 2136 1636
Size: 500 4083
StuI AGG'CCT
Cuts at: 430 856 430
Size: 426 4157
StyI C'CwwG_G
Cuts at: 696 1717 2071 696
Size: 1021 354 3208
Fragments arranged by size:
3208 1021 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 523 711 2166 2299 2758 2967 523
Size: 188 1455 133 459 209 2139
Fragments arranged by size:
2139 1455 459 209 188 133
TatI w'GTAC_w
Cuts at: 809 1361 4098 809
Size: 552 2737 1294
Fragments arranged by size:
2737 1294 552
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4100 4132 4100
Size: 32 4551
Tth111I GACn'n_nGTC
Cuts at: 2380 2380
Size: 4583
XbaI T'CTAG_A
Cuts at: 1614 1614
Size: 4583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 720 2078 720
Size: 1358 3225
XhoI C'TCGA_G
Cuts at: 830 830
Size: 4583
XmaI C'CCGG_G
Cuts at: 1603 1603
Size: 4583
XmnI GAAnn'nnTTC
Cuts at: 371 371
Size: 4583
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BbsI BcgI BfrBI BmgBI BmtI BsgI BsiWI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PsiI PspXI PsrI SanDI SexAI SfiI
SnaBI SrfI SspI SwaI ZraI