Restriction map for pF1KB4406
 (Circular) (Six-base) MAPSORT of: pF1KB4406.seq  Check: 412  from: 1  to: 9755



pF1KB4406  9755 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   3218    5646    5927    3218 
   Size:       2428     281    7046

  Fragments arranged by size: 

                7046    2428     281
 
AatII G_ACGT'C

Cuts at:   2540    6734    2540 
   Size:       4194    5561
 
AccI GT'mk_AC

Cuts at:   1376    4877    6793    1376 
   Size:       3501    1916    4338

  Fragments arranged by size: 

                4338    3501    1916
 
Acc65I G'GTAC_C

Cuts at:   4427    5844    6771    4427 
   Size:       1417     927    7411

  Fragments arranged by size: 

                7411    1417     927
 
AclI AA'CG_TT

Cuts at:   9595    9595 
   Size:       9755
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1267    1450    2821    4774    6388    7580    8012    8365    9027 
   Size:        183    1371    1953    1614    1192     432     353     662

Cuts at:   9027    1267 
   Size:       1995

  Fragments arranged by size: 

                1995    1953    1614    1371    1192     662     432     353
                 183
 
AfeI AGC'GCT

Cuts at:    354    2965    4576    4705     354 
   Size:       2611    1611     129    5404

  Fragments arranged by size: 

                5404    2611    1611     129
 
AflIII A'CryG_T

Cuts at:    515     846    1025    1664    4360    4555    6293    6386    7107 
   Size:        331     179     639    2696     195    1738      93     721

Cuts at:   7107    8907    9188     515 
   Size:       1800     281    1082

  Fragments arranged by size: 

                2696    1800    1738    1082     721     639     331     281
                 195     179      93
 
AleI CACnn'nnGTG

Cuts at:   1312    3701    4414    1312 
   Size:       2389     713    6653

  Fragments arranged by size: 

                6653    2389     713
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   3014    3014 
   Size:       9755
 
AlwNI CAG_nnn'CTG

Cuts at:    210    1876    3662    3975    6187    7060    8498     210 
   Size:       1666    1786     313    2212     873    1438    1467

  Fragments arranged by size: 

                2212    1786    1666    1467    1438     873     313
 
ApaI G_GGCC'C

Cuts at:   4355    4355 
   Size:       9755
 
ApaLI G'TGCA_C

Cuts at:    427    8593     427 
   Size:       8166    1589
 
ApoI r'AATT_y

Cuts at:    548    2218    6356    6759    9108    9364     548 
   Size:       1670    4138     403    2349     256     939

  Fragments arranged by size: 

                4138    2349    1670     939     403     256
 
AseI AT'TA_AT

Cuts at:     20    1145    1394      20 
   Size:       1125     249    8381

  Fragments arranged by size: 

                8381    1125     249
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       9755
 
AvaI C'yCGr_G

Cuts at:    367     470     965    2141    2433    6775     367 
   Size:        103     495    1176     292    4342    3347

  Fragments arranged by size: 

                4342    3347    1176     495     292     103
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   6280    6313    6280 
   Size:         33    9722
 
BamHI G'GATC_C

Cuts at:   3798    6780    3798 
   Size:       2982    6773
 
BanI G'GyrC_C

Cuts at:   4427    5844    6528    6771    7185    7433    7468    4427 
   Size:       1417     684     243     414     248      35    6714

  Fragments arranged by size: 

                6714    1417     684     414     248     243      35
 
BanII G_rGCy'C

Cuts at:    186     374    2748    3119    3926    4355    5130    6769    7799 
   Size:        188    2374     371     807     429     775    1639    1030

Cuts at:   7799     186 
   Size:       2142

  Fragments arranged by size: 

                2374    2142    1639    1030     807     775     429     371
                 188
 
BbeI G_GCGC'C

Cuts at:   7189    7437    7189 
   Size:        248    9507
 
BbsI GAAGACnn'nnnn_

Cuts at:    930    1064    1887    3334    4558    4915     930 
   Size:        134     823    1447    1224     357    5770

  Fragments arranged by size: 

                5770    1447    1224     823     357     134
 
BbvCI CC'TCA_GC

Cuts at:   2632    2632 
   Size:       9755
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    438     472    4015    4049     438 
   Size:         34    3543      34    6144

  Fragments arranged by size: 

                6144    3543      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3511    3762    7653    8709    9755    3511 
   Size:        251    3891    1056    1046    3511

  Fragments arranged by size: 

                3891    3511    1056    1046     251
 
BclI T'GATC_A

Cuts at:   2582    6216    7275    2582 
   Size:       3634    1059    5062

  Fragments arranged by size: 

                5062    3634    1059
 
BfrBI ATG'CAT

Cuts at:   6481    6481 
   Size:       9755
 
BglI GCCn_nnn'nGGC

Cuts at:    310    3293    3845    4604    6416     310 
   Size:       2983     552     759    1812    3649

  Fragments arranged by size: 

                3649    2983    1812     759     552
 
BglII A'GATC_T

Cuts at:   6018    6425    6502    7270    9307    6018 
   Size:        407      77     768    2037    6466

  Fragments arranged by size: 

                6466    2037     768     407      77
 
BlpI GC'TnA_GC

Cuts at:   1541    3049    6553    6867    1541 
   Size:       1508    3504     314    4429

  Fragments arranged by size: 

                4429    3504    1508     314
 
Bme1580I G_kGCm'C

Cuts at:    431    2762    3176    3737    4355    6533    7380    7473    8597 
   Size:       2331     414     561     618    2178     847      93    1124

Cuts at:   8597     431 
   Size:       1589

  Fragments arranged by size: 

                2331    2178    1589    1124     847     618     561     414
                  93
 
BmgBI CAC'GTC

Cuts at:   4556    5735    4556 
   Size:       1179    8576
 
BmrI ACTGGGnnnn_n'

Cuts at:    441     597     654    1116    2658    2799    3039    3465    5133 
   Size:        156      57     462    1542     141     240     426    1668

Cuts at:   5133    6139    6967    7048    7383    9527     441 
   Size:       1006     828      81     335    2144     669

  Fragments arranged by size: 

                2144    1668    1542    1006     828     669     462     426
                 335     240     156     141      81      57
 
BmtI G_CTAG'C

Cuts at:   1655    1655 
   Size:       9755
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   8122    8122 
   Size:       9755
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    458    1457    1552    1592    1706    1930    2620    2947    4936 
   Size:        999      95      40     114     224     690     327    1989

Cuts at:   4936    5062    5328    6627     458 
   Size:        126     266    1299    3586

  Fragments arranged by size: 

                3586    1989    1299     999     690     327     266     224
                 126     114      95      40
 
Bpu10I CC'TnA_GC

Cuts at:   2632    2632 
   Size:       9755
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1009    3289    3547    5635    6931    7810    8524    8822    1009 
   Size:       2280     258    2088    1296     879     714     298    1942

  Fragments arranged by size: 

                2280    2088    1942    1296     879     714     298     258
            
 
BsaI GGTCTCn'nnnn_

Cuts at:   1095    1730    1807    2789    4912    9459    1095 
   Size:        635      77     982    2123    4547    1391

  Fragments arranged by size: 

                4547    2123    1391     982     635      77
 
BsaAI yAC'GTr

Cuts at:    516    4361    4973    7108     516 
   Size:       3845     612    2135    3163

  Fragments arranged by size: 

                3845    3163    2135     612
 
BsaBI GATnn'nnATC

Cuts at:      6    3797    7269    9306       6 
   Size:       3791    3472    2037     455

  Fragments arranged by size: 

                3791    3472    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2537    6731    7186    7434    8136    9632    2537 
   Size:       4194     455     248     702    1496    2660

  Fragments arranged by size: 

                4194    2660    1496     702     455     248
 
BsaWI w'CCGG_w

Cuts at:    338    1574    2214    2291    6593    6944    7465    8143    8554 
   Size:       1236     640      77    4302     351     521     678     411

Cuts at:   8554    8701    8975     338 
   Size:        147     274    1118

  Fragments arranged by size: 

                4302    1236    1118     678     640     521     411     351
                 274     147      77
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   2312    2339    2342    2369    4651    4681    6625    6655    2312 
   Size:         27       3      27    2282      30    1944      30    5412

  Fragments arranged by size: 

                5412    2282    1944      30      30      27      27       3
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    724    1426    1549    1855    2336    3615    5120    5499    6657 
   Size:        702     123     306     481    1279    1505     379    1158

Cuts at:   6657     724 
   Size:       3822

  Fragments arranged by size: 

                3822    1505    1279    1158     702     481     379     306
                 123
 
BseYI C'CCAG_C

Cuts at:   2040    3746    6462    6648    7180    7938    8603    9011    2040 
   Size:       1706    2716     186     532     758     665     408    2784

  Fragments arranged by size: 

                2784    2716    1706     758     665     532     408     186
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    196     280    1965    2121    4460    5624    6494     196 
   Size:         84    1685     156    2339    1164     870    3457

  Fragments arranged by size: 

                3457    2339    1685    1164     870     156      84
 
BsiEI CG_ry'CG

Cuts at:    104    7297    7343    8573     104 
   Size:       7193      46    1230    1286

  Fragments arranged by size: 

                7193    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    186     431    1277    1891    2504    4697    6769    7547    7737 
   Size:        245     846     614     613    2193    2072     778     190

Cuts at:   7737    8597     186 
   Size:        860    1344

  Fragments arranged by size: 

                2193    2072    1344     860     846     778     614     613
                 245     190
 
BsmI GAATG_Cn'

Cuts at:   1586    4543    1586 
   Size:       2957    6798
 
Bsp1286I G_dGCh'C

Cuts at:    186     374     431    1277    1891    2504    2748    2762    3119 
   Size:        188      57     846     614     613     244      14     357

Cuts at:   3119    3176    3737    3926    4355    4697    5130    6533    6769 
   Size:         57     561     189     429     342     433    1403     236

Cuts at:   6769    7380    7473    7547    7737    7799    8597     186 
   Size:        611      93      74     190      62     798    1344

  Fragments arranged by size: 

                1403    1344     846     798     614     613     611     561
                 433     429     357     342     244     236     190     189
                 188      93      74      62      57      57      14
 
BspEI T'CCGG_A

Cuts at:    338    1574    2214     338 
   Size:       1236     640    7879

  Fragments arranged by size: 

                7879    1236     640
 
BspHI T'CATG_A

Cuts at:    833    8187     833 
   Size:       7354    2401
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   3218    4405    5646    5927    6805    7019    7321    7702    3218 
   Size:       1187    1241     281     878     214     302     381    5271

  Fragments arranged by size: 

                5271    1241    1187     878     381     302     281     214
            
 
BsrBI CCG'CTC

Cuts at:     50     336    2022    2940    3623    8047    8101    9585    9750 
   Size:        286    1686     918     683    4424      54    1484     165

Cuts at:   9750      50 
   Size:         55

  Fragments arranged by size: 

                4424    1686    1484     918     683     286     165      55
                  54
 
BsrDI GCAATG_nn'

Cuts at:   1379    1760    3002    3168    3831    7667    1379 
   Size:        381    1242     166     663    3836    3467

  Fragments arranged by size: 

                3836    3467    1242     663     381     166
 
BsrFI r'CCGG_y

Cuts at:   1297    2112    5695    7753    7934    1297 
   Size:        815    3583    2058     181    3118

  Fragments arranged by size: 

                3583    3118    2058     815     181
 
BsrGI T'GTAC_A

Cuts at:   1667    4772    6581    1667 
   Size:       3105    1809    4841

  Fragments arranged by size: 

                4841    3105    1809
 
BssHII G'CGCG_C

Cuts at:    224     292     224 
   Size:         68    9687
 
BssSI C'ACGA_G

Cuts at:    253    2755    8026    8734     253 
   Size:       2502    5271     708    1274

  Fragments arranged by size: 

                5271    2502    1274     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:    210    6190    7132     210 
   Size:       5980     942    2833

  Fragments arranged by size: 

                5980    2833     942
 
BstBI TT'CG_AA

Cuts at:   4535    8116    4535 
   Size:       3581    6174
 
BstEII G'GTnAC_C

Cuts at:   2006    2006 
   Size:       9755
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2748    3490    5146    6430    2748 
   Size:        742    1656    1284    6073

  Fragments arranged by size: 

                6073    1656    1284     742
 
BstYI r'GATC_y

Cuts at:      1    2211    3798    4059    6018    6425    6502    6780    7247 
   Size:       2210    1587     261    1959     407      77     278     467

Cuts at:   7247    7270    7605    7851    8157    8169    8255    9307       1 
   Size:         23     335     246     306      12      86    1052     449

  Fragments arranged by size: 

                2210    1959    1587    1052     467     449     407     335
                 306     278     261     246      86      77      23      12
            
 
Bsu36I CC'TnA_GG

Cuts at:   3290    5038    3290 
   Size:       1748    8007
 
BtgI C'CryG_G

Cuts at:    107    1740    1958    3986    5145    5411    5948    6737     107 
   Size:       1633     218    2028    1159     266     537     789    3125

  Fragments arranged by size: 

                3125    2028    1633    1159     789     537     266     218
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1143    1605    3443    4530    4754    7705    7886    8049    1143 
   Size:        462    1838    1087     224    2951     181     163    2849

  Fragments arranged by size: 

                2951    2849    1838    1087     462     224     181     163
            
 
BtsI GCAGTG_nn'

Cuts at:    166     510    1065    1308    1686    2483    3258    3735    3915 
   Size:        344     555     243     378     797     775     477     180

Cuts at:   3915    3976    4245    4395    4484    4761    5684    6998     166 
   Size:         61     269     150      89     277     923    1314    2923

  Fragments arranged by size: 

                2923    1314     923     797     775     555     477     378
                 344     277     269     243     180     150      89      61
            
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   3057    3092    3396    3431    4491    4526    4576    4611    3057 
   Size:         35     304      35    1060      35      50      35    8201

  Fragments arranged by size: 

                8201    1060     304      50      35      35      35      35
            
 
DraI TTT'AAA

Cuts at:   6251    6354    6754    6251 
   Size:        103     400    9252

  Fragments arranged by size: 

                9252     400     103
 
DraIII CAC_nnn'GTG

Cuts at:   1356    2853    1356 
   Size:       1497    8258
 
DrdI GACnn_nn'nnGTC

Cuts at:    395    7461    8805     395 
   Size:       7066    1344    1345

  Fragments arranged by size: 

                7066    1345    1344
 
EaeI y'GGCC_r

Cuts at:    819    1098    1757    2955    4242    4584    4605    7340    7514 
   Size:        279     659    1198    1287     342      21    2735     174

Cuts at:   7514    7905    7932    9277     819 
   Size:        391      27    1345    1297

  Fragments arranged by size: 

                2735    1345    1297    1287    1198     659     391     342
                 279     174      27      21
 
EagI C'GGCC_G

Cuts at:   7340    7340 
   Size:       9755
 
EarI CTCTTCn'nnn_

Cuts at:    701    1879    2369    3323    5758    7778    7988     701 
   Size:       1178     490     954    2435    2020     210    2468

  Fragments arranged by size: 

                2468    2435    2020    1178     954     490     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1760    2082    7963    8705    8851    9565    1760 
   Size:        322    5881     742     146     714    1950

  Fragments arranged by size: 

                5881    1950     742     714     322     146
 
EcoICRI GAG'CTC

Cuts at:    184    6767     184 
   Size:       6583    3172
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    458    1267    1450    1457    1552    1592    1706    1930    2620 
   Size:        809     183       7      95      40     114     224     690

Cuts at:   2620    2821    2947    4774    4936    5062    5328    6388    6627 
   Size:        201     126    1827     162     126     266    1060     239

Cuts at:   6627    7580    8012    8365    9027     458 
   Size:        953     432     353     662    1186

  Fragments arranged by size: 

                1827    1186    1060     953     809     690     662     432
                 353     266     239     224     201     183     162     126
                 126     114      95      40       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1314    1594    3202    4833    5208    1314 
   Size:        280    1608    1631     375    5861

  Fragments arranged by size: 

                5861    1631    1608     375     280
 
EcoO109I rG'GnC_Cy

Cuts at:    779    3080    3270    4352    4394    5042    6894     779 
   Size:       2301     190    1082      42     648    1852    3640

  Fragments arranged by size: 

                3640    2301    1852    1082     648     190      42
 
EcoRI G'AATT_C

Cuts at:   6759    6759 
   Size:       9755
 
EcoRV GAT'ATC

Cuts at:   5364    5424    5364 
   Size:         60    9695
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    582    1003    5598    6263    7774     582 
   Size:        421    4595     665    1511    2563

  Fragments arranged by size: 

                4595    2563    1511     665     421
 
FspI TGC'GCA

Cuts at:   7536    7536 
   Size:       9755
 
HaeII r_GCGC'y

Cuts at:    356    1548    2550    2967    4578    4707    7189    7437    8667 
   Size:       1192    1002     417    1611     129    2482     248    1230

Cuts at:   8667    9438     356 
   Size:        771     673

  Fragments arranged by size: 

                2482    1611    1230    1192    1002     771     673     417
                 248     129
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1415    1447    1484    1516    2339    2371    2416    2448    2696 
   Size:         32      37      32     823      32      45      32     248

Cuts at:   2696    2728    3025    3057    3229    3261    4103    4135    5305 
   Size:         32     297      32     172      32     842      32    1170

Cuts at:   5305    5337    6451    6483    7597    7627    7629    7659    1415 
   Size:         32    1114      32    1114      30       2      30    3511

  Fragments arranged by size: 

                3511    1170    1114    1114     842     823     297     248
                 172      45      37      32      32      32      32      32
                  32      32      32      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   3807    4766    5253    6794    3807 
   Size:        959     487    1541    6768

  Fragments arranged by size: 

                6768    1541     959     487
 
HindIII A'AGCT_T

Cuts at:   2612    5397    2612 
   Size:       2785    6970
 
KasI G'GCGC_C

Cuts at:   7185    7433    7185 
   Size:        248    9507
 
KpnI G_GTAC'C

Cuts at:   4431    5848    6775    4431 
   Size:       1417     927    7411

  Fragments arranged by size: 

                7411    1417     927
 
MfeI C'AATT_G

Cuts at:   1503    4620    6005    1503 
   Size:       3117    1385    5253

  Fragments arranged by size: 

                5253    3117    1385
 
MluI A'CGCG_T

Cuts at:   9188    9188 
   Size:       9755
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    779    2150    2546    2727    3737    4872    6074    6150    6386 
   Size:       1371     396     181    1010    1135    1202      76     236

Cuts at:   6386    8514    8698     779 
   Size:       2128     184    1836

  Fragments arranged by size: 

                2128    1836    1371    1202    1135    1010     396     236
                 184     181      76
 
MscI TGG'CCA

Cuts at:    821    2957    4244    7516     821 
   Size:       2136    1287    3272    3060

  Fragments arranged by size: 

                3272    3060    2136    1287
 
MslI CAynn'nnrTG

Cuts at:    379    1312    2887    2996    3124    3701    3875    4414    5144 
   Size:        933    1575     109     128     577     174     539     730

Cuts at:   5144    5921    6266    7744    7871     379 
   Size:        777     345    1478     127    2263

  Fragments arranged by size: 

                2263    1575    1478     933     777     730     577     539
                 345     174     128     127     109
 
MspA1I CmG'CkG

Cuts at:    727     798    1934    2640    3750    3836    4382    4801    5082 
   Size:         71    1136     706    1110      86     546     419     281

Cuts at:   5082    6866    7180    7540    8324    8569     727 
   Size:       1784     314     360     784     245    1913

  Fragments arranged by size: 

                1913    1784    1136    1110     784     706     546     419
                 360     314     281     245      86      71
 
NaeI GCC'GGC

Cuts at:   1299    7936    1299 
   Size:       6637    3118
 
NarI GG'CG_CC

Cuts at:   7186    7434    7186 
   Size:        248    9507
 
NcoI C'CATG_G

Cuts at:    107    3986    5145    5411    6737     107 
   Size:       3879    1159     266    1326    3125

  Fragments arranged by size: 

                3879    3125    1326    1159     266
 
NdeI CA'TA_TG

Cuts at:   4903    4903 
   Size:       9755
 
NgoMIV G'CCGG_C

Cuts at:   1297    7934    1297 
   Size:       6637    3118
 
NheI G'CTAG_C

Cuts at:   1651    1651 
   Size:       9755
 
NruI TCG'CGA

Cuts at:    138     138 
   Size:       9755
 
NsiI A_TGCA'T

Cuts at:   6483    6483 
   Size:       9755
 
NspI r_CATG'y

Cuts at:    850    1029    2087    3615    5614    5926    6297    6808    7308 
   Size:        179    1058    1528    1999     312     371     511     500

Cuts at:   7308    8911     850 
   Size:       1603    1694

  Fragments arranged by size: 

                1999    1694    1603    1528    1058     511     500     371
                 312     179
 
PasI CC'CwG_GG

Cuts at:   1518    1767    2741    5093    1518 
   Size:        249     974    2352    6180

  Fragments arranged by size: 

                6180    2352     974     249
 
PciI A'CATG_T

Cuts at:    846    1025    6293    8907     846 
   Size:        179    5268    2614    1694

  Fragments arranged by size: 

                5268    2614    1694     179
 
PflMI CCAn_nnn'nTGG

Cuts at:   1356    1773    3987    4262    5198    1356 
   Size:        417    2214     275     936    5913

  Fragments arranged by size: 

                5913    2214     936     417     275
 
PfoI T'CCnGG_A

Cuts at:   1710    4718    5891    1710 
   Size:       3008    1173    5574

  Fragments arranged by size: 

                5574    3008    1173
 
PmeI GTTT'AAAC

Cuts at:   6754    6754 
   Size:       9755
 
PmlI CAC'GTG

Cuts at:   4361    4361 
   Size:       9755
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2368    2400    8201    8233    2368 
   Size:         32    5801      32    3890

  Fragments arranged by size: 

                5801    3890      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    779    3080    3270    4394    5042     779 
   Size:       2301     190    1124     648    5492

  Fragments arranged by size: 

                5492    2301    1124     648     190
 
PspOMI G'GGCC_C

Cuts at:   4351    4351 
   Size:       9755
 
PstI C_TGCA'G

Cuts at:    365    3229    3364    3604    3658    4387    4756    5679    6605 
   Size:       2864     135     240      54     729     369     923     926

Cuts at:   6605    6802    7487     365 
   Size:        197     685    2633

  Fragments arranged by size: 

                2864    2633     926     923     729     685     369     240
                 197     135      54
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       9755
 
PvuII CAG'CTG

Cuts at:    727     798    1934    2640    4801    5082    7180    7540     727 
   Size:         71    1136     706    2161     281    2098     360    2942

  Fragments arranged by size: 

                2942    2161    2098    1136     706     360     281      71
            
 
RsrII CG'GwC_CG

Cuts at:   7950    7950 
   Size:       9755
 
SacI G_AGCT'C

Cuts at:    186    6769     186 
   Size:       6583    3172
 
SalI G'TCGA_C

Cuts at:   6792    6792 
   Size:       9755
 
SanDI GG'GwC_CC

Cuts at:   3080    3080 
   Size:       9755
 
SapI GCTCTTCn'nnn_

Cuts at:   1879    5758    7778    7988    1879 
   Size:       3879    2020     210    3646

  Fragments arranged by size: 

                3879    3646    2020     210
 
SbfI CC_TGCA'GG

Cuts at:   6802    6802 
   Size:       9755
 
ScaI AGT'ACT

Cuts at:   9272    9272 
   Size:       9755
 
SexAI A'CCwGG_T

Cuts at:   1178    2849    1178 
   Size:       1671    8084
 
SfcI C'TryA_G

Cuts at:     33     361    3225    3360    3600    3654    4383    4752    4881 
   Size:        328    2864     135     240      54     729     369     129

Cuts at:   4881    5675    6601    6636    6798    7483    8451    8642      33 
   Size:        794     926      35     162     685     968     191    1146

  Fragments arranged by size: 

                2864    1146     968     926     794     729     685     369
                 328     240     191     162     135     129      54      35
            
 
SfoI GGC'GCC

Cuts at:   7187    7435    7187 
   Size:        248    9507
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       9755
 
SmaI CCC'GGG

Cuts at:    967    2143    6777     967 
   Size:       1176    4634    3945

  Fragments arranged by size: 

                4634    3945    1176
 
SmlI C'TyrA_G

Cuts at:    988    3304    3526    5650    6910    7825    8539    8801     988 
   Size:       2316     222    2124    1260     915     714     262    1942

  Fragments arranged by size: 

                2316    2124    1942    1260     915     714     262     222
            
 
SpeI A'CTAG_T

Cuts at:     67    5287      67 
   Size:       5220    4535
 
SphI G_CATG'C

Cuts at:   6808    7308    6808 
   Size:        500    9255
 
SspI AAT'ATT

Cuts at:   1153    1153 
   Size:       9755
 
StuI AGG'CCT

Cuts at:    751    1613    3709    4684    4750     751 
   Size:        862    2096     975      66    5756

  Fragments arranged by size: 

                5756    2096     975     862      66
 
StyI C'CwwG_G

Cuts at:    107     464    1262    1310    1595    1967    2531    3203    3986 
   Size:        357     798      48     285     372     564     672     783

Cuts at:   3986    5145    5411    6327    6737    6889    7243     107 
   Size:       1159     266     916     410     152     354    2619

  Fragments arranged by size: 

                2619    1159     916     798     783     672     564     410
                 372     357     354     285     266     152      48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1416    3484    4404    4519    4538    5158    5485    7338    7471 
   Size:       2068     920     115      19     620     327    1853     133

Cuts at:   7471    7930    8139    1416 
   Size:        459     209    3032

  Fragments arranged by size: 

                3032    2068    1853     920     620     459     327     209
                 133     115      19
 
TatI w'GTAC_w

Cuts at:    946    1022    1667    3593    4772    4779    4986    5162    6581 
   Size:         76     645    1926    1179       7     207     176    1419

Cuts at:   6581    9270     946 
   Size:       2689    1431

  Fragments arranged by size: 

                2689    1926    1431    1419    1179     645     207     176
                  76       7
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1122    1154    2140    2172    2854    2884    2886    2916    3262 
   Size:         32     986      32     682      30       2      30     346

Cuts at:   3262    3294    3937    3969    4563    4595    4651    4683    5348 
   Size:         32     643      32     594      32      56      32     665

Cuts at:   5348    5380    6625    6657    9272    9304    1122 
   Size:         32    1245      32    2615      32    1573

  Fragments arranged by size: 

                2615    1573    1245     986     682     665     643     594
                 346      56      32      32      32      32      32      32
                  32      32      32      30      30       2
 
Tth111I GACn'n_nGTC

Cuts at:   7552    7552 
   Size:       9755
 
XbaI T'CTAG_A

Cuts at:   6786    6786 
   Size:       9755
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    762    1632    1965    3459    4105    5580    6369    7250     762 
   Size:        870     333    1494     646    1475     789     881    3267

  Fragments arranged by size: 

                3267    1494    1475     881     870     789     646     333
            
 
XmaI C'CCGG_G

Cuts at:    965    2141    6775     965 
   Size:       1176    4634    3945

  Fragments arranged by size: 

                4634    3945    1176
 
XmnI GAAnn'nnTTC

Cuts at:   1825    2383    3133    5452    1825 
   Size:        558     750    2319    6128

  Fragments arranged by size: 

                6128    2319     750     558
 
ZraI GAC'GTC

Cuts at:   2538    6732    2538 
   Size:       4194    5561
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NruI     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

    AflII     AgeI     AhdI     AloI     AscI    AvrII    BsiWI    BsmBI
  BstZ17I     ClaI     FseI    FspAI     HpaI     NotI     PacI    PshAI
     PsiI    PspXI     PsrI    SacII     SfiI    SgrAI    SnaBI     SrfI
     SwaI     XhoI
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