(Circular) (Six-base) MAPSORT of: pF1KB4406.seq Check: 412 from: 1 to: 9755 pF1KB4406 9755 bp With 182 enzymes: SgfI * June 20, 2008 10:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3218 5646 5927 3218 Size: 2428 281 7046 Fragments arranged by size: 7046 2428 281 AatII G_ACGT'C Cuts at: 2540 6734 2540 Size: 4194 5561 AccI GT'mk_AC Cuts at: 1376 4877 6793 1376 Size: 3501 1916 4338 Fragments arranged by size: 4338 3501 1916 Acc65I G'GTAC_C Cuts at: 4427 5844 6771 4427 Size: 1417 927 7411 Fragments arranged by size: 7411 1417 927 AclI AA'CG_TT Cuts at: 9595 9595 Size: 9755 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1267 1450 2821 4774 6388 7580 8012 8365 9027 Size: 183 1371 1953 1614 1192 432 353 662 Cuts at: 9027 1267 Size: 1995 Fragments arranged by size: 1995 1953 1614 1371 1192 662 432 353 183 AfeI AGC'GCT Cuts at: 354 2965 4576 4705 354 Size: 2611 1611 129 5404 Fragments arranged by size: 5404 2611 1611 129 AflIII A'CryG_T Cuts at: 515 846 1025 1664 4360 4555 6293 6386 7107 Size: 331 179 639 2696 195 1738 93 721 Cuts at: 7107 8907 9188 515 Size: 1800 281 1082 Fragments arranged by size: 2696 1800 1738 1082 721 639 331 281 195 179 93 AleI CACnn'nnGTG Cuts at: 1312 3701 4414 1312 Size: 2389 713 6653 Fragments arranged by size: 6653 2389 713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 3014 3014 Size: 9755 AlwNI CAG_nnn'CTG Cuts at: 210 1876 3662 3975 6187 7060 8498 210 Size: 1666 1786 313 2212 873 1438 1467 Fragments arranged by size: 2212 1786 1666 1467 1438 873 313 ApaI G_GGCC'C Cuts at: 4355 4355 Size: 9755 ApaLI G'TGCA_C Cuts at: 427 8593 427 Size: 8166 1589 ApoI r'AATT_y Cuts at: 548 2218 6356 6759 9108 9364 548 Size: 1670 4138 403 2349 256 939 Fragments arranged by size: 4138 2349 1670 939 403 256 AseI AT'TA_AT Cuts at: 20 1145 1394 20 Size: 1125 249 8381 Fragments arranged by size: 8381 1125 249 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 9755 AvaI C'yCGr_G Cuts at: 367 470 965 2141 2433 6775 367 Size: 103 495 1176 292 4342 3347 Fragments arranged by size: 4342 3347 1176 495 292 103 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6280 6313 6280 Size: 33 9722 BamHI G'GATC_C Cuts at: 3798 6780 3798 Size: 2982 6773 BanI G'GyrC_C Cuts at: 4427 5844 6528 6771 7185 7433 7468 4427 Size: 1417 684 243 414 248 35 6714 Fragments arranged by size: 6714 1417 684 414 248 243 35 BanII G_rGCy'C Cuts at: 186 374 2748 3119 3926 4355 5130 6769 7799 Size: 188 2374 371 807 429 775 1639 1030 Cuts at: 7799 186 Size: 2142 Fragments arranged by size: 2374 2142 1639 1030 807 775 429 371 188 BbeI G_GCGC'C Cuts at: 7189 7437 7189 Size: 248 9507 BbsI GAAGACnn'nnnn_ Cuts at: 930 1064 1887 3334 4558 4915 930 Size: 134 823 1447 1224 357 5770 Fragments arranged by size: 5770 1447 1224 823 357 134 BbvCI CC'TCA_GC Cuts at: 2632 2632 Size: 9755 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 438 472 4015 4049 438 Size: 34 3543 34 6144 Fragments arranged by size: 6144 3543 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3511 3762 7653 8709 9755 3511 Size: 251 3891 1056 1046 3511 Fragments arranged by size: 3891 3511 1056 1046 251 BclI T'GATC_A Cuts at: 2582 6216 7275 2582 Size: 3634 1059 5062 Fragments arranged by size: 5062 3634 1059 BfrBI ATG'CAT Cuts at: 6481 6481 Size: 9755 BglI GCCn_nnn'nGGC Cuts at: 310 3293 3845 4604 6416 310 Size: 2983 552 759 1812 3649 Fragments arranged by size: 3649 2983 1812 759 552 BglII A'GATC_T Cuts at: 6018 6425 6502 7270 9307 6018 Size: 407 77 768 2037 6466 Fragments arranged by size: 6466 2037 768 407 77 BlpI GC'TnA_GC Cuts at: 1541 3049 6553 6867 1541 Size: 1508 3504 314 4429 Fragments arranged by size: 4429 3504 1508 314 Bme1580I G_kGCm'C Cuts at: 431 2762 3176 3737 4355 6533 7380 7473 8597 Size: 2331 414 561 618 2178 847 93 1124 Cuts at: 8597 431 Size: 1589 Fragments arranged by size: 2331 2178 1589 1124 847 618 561 414 93 BmgBI CAC'GTC Cuts at: 4556 5735 4556 Size: 1179 8576 BmrI ACTGGGnnnn_n' Cuts at: 441 597 654 1116 2658 2799 3039 3465 5133 Size: 156 57 462 1542 141 240 426 1668 Cuts at: 5133 6139 6967 7048 7383 9527 441 Size: 1006 828 81 335 2144 669 Fragments arranged by size: 2144 1668 1542 1006 828 669 462 426 335 240 156 141 81 57 BmtI G_CTAG'C Cuts at: 1655 1655 Size: 9755 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8122 8122 Size: 9755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 458 1457 1552 1592 1706 1930 2620 2947 4936 Size: 999 95 40 114 224 690 327 1989 Cuts at: 4936 5062 5328 6627 458 Size: 126 266 1299 3586 Fragments arranged by size: 3586 1989 1299 999 690 327 266 224 126 114 95 40 Bpu10I CC'TnA_GC Cuts at: 2632 2632 Size: 9755 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1009 3289 3547 5635 6931 7810 8524 8822 1009 Size: 2280 258 2088 1296 879 714 298 1942 Fragments arranged by size: 2280 2088 1942 1296 879 714 298 258 BsaI GGTCTCn'nnnn_ Cuts at: 1095 1730 1807 2789 4912 9459 1095 Size: 635 77 982 2123 4547 1391 Fragments arranged by size: 4547 2123 1391 982 635 77 BsaAI yAC'GTr Cuts at: 516 4361 4973 7108 516 Size: 3845 612 2135 3163 Fragments arranged by size: 3845 3163 2135 612 BsaBI GATnn'nnATC Cuts at: 6 3797 7269 9306 6 Size: 3791 3472 2037 455 Fragments arranged by size: 3791 3472 2037 455 BsaHI Gr'CG_yC Cuts at: 2537 6731 7186 7434 8136 9632 2537 Size: 4194 455 248 702 1496 2660 Fragments arranged by size: 4194 2660 1496 702 455 248 BsaWI w'CCGG_w Cuts at: 338 1574 2214 2291 6593 6944 7465 8143 8554 Size: 1236 640 77 4302 351 521 678 411 Cuts at: 8554 8701 8975 338 Size: 147 274 1118 Fragments arranged by size: 4302 1236 1118 678 640 521 411 351 274 147 77 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 2312 2339 2342 2369 4651 4681 6625 6655 2312 Size: 27 3 27 2282 30 1944 30 5412 Fragments arranged by size: 5412 2282 1944 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 724 1426 1549 1855 2336 3615 5120 5499 6657 Size: 702 123 306 481 1279 1505 379 1158 Cuts at: 6657 724 Size: 3822 Fragments arranged by size: 3822 1505 1279 1158 702 481 379 306 123 BseYI C'CCAG_C Cuts at: 2040 3746 6462 6648 7180 7938 8603 9011 2040 Size: 1706 2716 186 532 758 665 408 2784 Fragments arranged by size: 2784 2716 1706 758 665 532 408 186 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 196 280 1965 2121 4460 5624 6494 196 Size: 84 1685 156 2339 1164 870 3457 Fragments arranged by size: 3457 2339 1685 1164 870 156 84 BsiEI CG_ry'CG Cuts at: 104 7297 7343 8573 104 Size: 7193 46 1230 1286 Fragments arranged by size: 7193 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 186 431 1277 1891 2504 4697 6769 7547 7737 Size: 245 846 614 613 2193 2072 778 190 Cuts at: 7737 8597 186 Size: 860 1344 Fragments arranged by size: 2193 2072 1344 860 846 778 614 613 245 190 BsmI GAATG_Cn' Cuts at: 1586 4543 1586 Size: 2957 6798 Bsp1286I G_dGCh'C Cuts at: 186 374 431 1277 1891 2504 2748 2762 3119 Size: 188 57 846 614 613 244 14 357 Cuts at: 3119 3176 3737 3926 4355 4697 5130 6533 6769 Size: 57 561 189 429 342 433 1403 236 Cuts at: 6769 7380 7473 7547 7737 7799 8597 186 Size: 611 93 74 190 62 798 1344 Fragments arranged by size: 1403 1344 846 798 614 613 611 561 433 429 357 342 244 236 190 189 188 93 74 62 57 57 14 BspEI T'CCGG_A Cuts at: 338 1574 2214 338 Size: 1236 640 7879 Fragments arranged by size: 7879 1236 640 BspHI T'CATG_A Cuts at: 833 8187 833 Size: 7354 2401 BspMI ACCTGCnnnn'nnnn_ Cuts at: 3218 4405 5646 5927 6805 7019 7321 7702 3218 Size: 1187 1241 281 878 214 302 381 5271 Fragments arranged by size: 5271 1241 1187 878 381 302 281 214 BsrBI CCG'CTC Cuts at: 50 336 2022 2940 3623 8047 8101 9585 9750 Size: 286 1686 918 683 4424 54 1484 165 Cuts at: 9750 50 Size: 55 Fragments arranged by size: 4424 1686 1484 918 683 286 165 55 54 BsrDI GCAATG_nn' Cuts at: 1379 1760 3002 3168 3831 7667 1379 Size: 381 1242 166 663 3836 3467 Fragments arranged by size: 3836 3467 1242 663 381 166 BsrFI r'CCGG_y Cuts at: 1297 2112 5695 7753 7934 1297 Size: 815 3583 2058 181 3118 Fragments arranged by size: 3583 3118 2058 815 181 BsrGI T'GTAC_A Cuts at: 1667 4772 6581 1667 Size: 3105 1809 4841 Fragments arranged by size: 4841 3105 1809 BssHII G'CGCG_C Cuts at: 224 292 224 Size: 68 9687 BssSI C'ACGA_G Cuts at: 253 2755 8026 8734 253 Size: 2502 5271 708 1274 Fragments arranged by size: 5271 2502 1274 708 BstAPI GCAn_nnn'nTGC Cuts at: 210 6190 7132 210 Size: 5980 942 2833 Fragments arranged by size: 5980 2833 942 BstBI TT'CG_AA Cuts at: 4535 8116 4535 Size: 3581 6174 BstEII G'GTnAC_C Cuts at: 2006 2006 Size: 9755 BstXI CCAn_nnnn'nTGG Cuts at: 2748 3490 5146 6430 2748 Size: 742 1656 1284 6073 Fragments arranged by size: 6073 1656 1284 742 BstYI r'GATC_y Cuts at: 1 2211 3798 4059 6018 6425 6502 6780 7247 Size: 2210 1587 261 1959 407 77 278 467 Cuts at: 7247 7270 7605 7851 8157 8169 8255 9307 1 Size: 23 335 246 306 12 86 1052 449 Fragments arranged by size: 2210 1959 1587 1052 467 449 407 335 306 278 261 246 86 77 23 12 Bsu36I CC'TnA_GG Cuts at: 3290 5038 3290 Size: 1748 8007 BtgI C'CryG_G Cuts at: 107 1740 1958 3986 5145 5411 5948 6737 107 Size: 1633 218 2028 1159 266 537 789 3125 Fragments arranged by size: 3125 2028 1633 1159 789 537 266 218 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1143 1605 3443 4530 4754 7705 7886 8049 1143 Size: 462 1838 1087 224 2951 181 163 2849 Fragments arranged by size: 2951 2849 1838 1087 462 224 181 163 BtsI GCAGTG_nn' Cuts at: 166 510 1065 1308 1686 2483 3258 3735 3915 Size: 344 555 243 378 797 775 477 180 Cuts at: 3915 3976 4245 4395 4484 4761 5684 6998 166 Size: 61 269 150 89 277 923 1314 2923 Fragments arranged by size: 2923 1314 923 797 775 555 477 378 344 277 269 243 180 150 89 61 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 3057 3092 3396 3431 4491 4526 4576 4611 3057 Size: 35 304 35 1060 35 50 35 8201 Fragments arranged by size: 8201 1060 304 50 35 35 35 35 DraI TTT'AAA Cuts at: 6251 6354 6754 6251 Size: 103 400 9252 Fragments arranged by size: 9252 400 103 DraIII CAC_nnn'GTG Cuts at: 1356 2853 1356 Size: 1497 8258 DrdI GACnn_nn'nnGTC Cuts at: 395 7461 8805 395 Size: 7066 1344 1345 Fragments arranged by size: 7066 1345 1344 EaeI y'GGCC_r Cuts at: 819 1098 1757 2955 4242 4584 4605 7340 7514 Size: 279 659 1198 1287 342 21 2735 174 Cuts at: 7514 7905 7932 9277 819 Size: 391 27 1345 1297 Fragments arranged by size: 2735 1345 1297 1287 1198 659 391 342 279 174 27 21 EagI C'GGCC_G Cuts at: 7340 7340 Size: 9755 EarI CTCTTCn'nnn_ Cuts at: 701 1879 2369 3323 5758 7778 7988 701 Size: 1178 490 954 2435 2020 210 2468 Fragments arranged by size: 2468 2435 2020 1178 954 490 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1760 2082 7963 8705 8851 9565 1760 Size: 322 5881 742 146 714 1950 Fragments arranged by size: 5881 1950 742 714 322 146 EcoICRI GAG'CTC Cuts at: 184 6767 184 Size: 6583 3172 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 458 1267 1450 1457 1552 1592 1706 1930 2620 Size: 809 183 7 95 40 114 224 690 Cuts at: 2620 2821 2947 4774 4936 5062 5328 6388 6627 Size: 201 126 1827 162 126 266 1060 239 Cuts at: 6627 7580 8012 8365 9027 458 Size: 953 432 353 662 1186 Fragments arranged by size: 1827 1186 1060 953 809 690 662 432 353 266 239 224 201 183 162 126 126 114 95 40 7 EcoNI CCTnn'n_nnAGG Cuts at: 1314 1594 3202 4833 5208 1314 Size: 280 1608 1631 375 5861 Fragments arranged by size: 5861 1631 1608 375 280 EcoO109I rG'GnC_Cy Cuts at: 779 3080 3270 4352 4394 5042 6894 779 Size: 2301 190 1082 42 648 1852 3640 Fragments arranged by size: 3640 2301 1852 1082 648 190 42 EcoRI G'AATT_C Cuts at: 6759 6759 Size: 9755 EcoRV GAT'ATC Cuts at: 5364 5424 5364 Size: 60 9695 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 582 1003 5598 6263 7774 582 Size: 421 4595 665 1511 2563 Fragments arranged by size: 4595 2563 1511 665 421 FspI TGC'GCA Cuts at: 7536 7536 Size: 9755 HaeII r_GCGC'y Cuts at: 356 1548 2550 2967 4578 4707 7189 7437 8667 Size: 1192 1002 417 1611 129 2482 248 1230 Cuts at: 8667 9438 356 Size: 771 673 Fragments arranged by size: 2482 1611 1230 1192 1002 771 673 417 248 129 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1415 1447 1484 1516 2339 2371 2416 2448 2696 Size: 32 37 32 823 32 45 32 248 Cuts at: 2696 2728 3025 3057 3229 3261 4103 4135 5305 Size: 32 297 32 172 32 842 32 1170 Cuts at: 5305 5337 6451 6483 7597 7627 7629 7659 1415 Size: 32 1114 32 1114 30 2 30 3511 Fragments arranged by size: 3511 1170 1114 1114 842 823 297 248 172 45 37 32 32 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 3807 4766 5253 6794 3807 Size: 959 487 1541 6768 Fragments arranged by size: 6768 1541 959 487 HindIII A'AGCT_T Cuts at: 2612 5397 2612 Size: 2785 6970 KasI G'GCGC_C Cuts at: 7185 7433 7185 Size: 248 9507 KpnI G_GTAC'C Cuts at: 4431 5848 6775 4431 Size: 1417 927 7411 Fragments arranged by size: 7411 1417 927 MfeI C'AATT_G Cuts at: 1503 4620 6005 1503 Size: 3117 1385 5253 Fragments arranged by size: 5253 3117 1385 MluI A'CGCG_T Cuts at: 9188 9188 Size: 9755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 779 2150 2546 2727 3737 4872 6074 6150 6386 Size: 1371 396 181 1010 1135 1202 76 236 Cuts at: 6386 8514 8698 779 Size: 2128 184 1836 Fragments arranged by size: 2128 1836 1371 1202 1135 1010 396 236 184 181 76 MscI TGG'CCA Cuts at: 821 2957 4244 7516 821 Size: 2136 1287 3272 3060 Fragments arranged by size: 3272 3060 2136 1287 MslI CAynn'nnrTG Cuts at: 379 1312 2887 2996 3124 3701 3875 4414 5144 Size: 933 1575 109 128 577 174 539 730 Cuts at: 5144 5921 6266 7744 7871 379 Size: 777 345 1478 127 2263 Fragments arranged by size: 2263 1575 1478 933 777 730 577 539 345 174 128 127 109 MspA1I CmG'CkG Cuts at: 727 798 1934 2640 3750 3836 4382 4801 5082 Size: 71 1136 706 1110 86 546 419 281 Cuts at: 5082 6866 7180 7540 8324 8569 727 Size: 1784 314 360 784 245 1913 Fragments arranged by size: 1913 1784 1136 1110 784 706 546 419 360 314 281 245 86 71 NaeI GCC'GGC Cuts at: 1299 7936 1299 Size: 6637 3118 NarI GG'CG_CC Cuts at: 7186 7434 7186 Size: 248 9507 NcoI C'CATG_G Cuts at: 107 3986 5145 5411 6737 107 Size: 3879 1159 266 1326 3125 Fragments arranged by size: 3879 3125 1326 1159 266 NdeI CA'TA_TG Cuts at: 4903 4903 Size: 9755 NgoMIV G'CCGG_C Cuts at: 1297 7934 1297 Size: 6637 3118 NheI G'CTAG_C Cuts at: 1651 1651 Size: 9755 NruI TCG'CGA Cuts at: 138 138 Size: 9755 NsiI A_TGCA'T Cuts at: 6483 6483 Size: 9755 NspI r_CATG'y Cuts at: 850 1029 2087 3615 5614 5926 6297 6808 7308 Size: 179 1058 1528 1999 312 371 511 500 Cuts at: 7308 8911 850 Size: 1603 1694 Fragments arranged by size: 1999 1694 1603 1528 1058 511 500 371 312 179 PasI CC'CwG_GG Cuts at: 1518 1767 2741 5093 1518 Size: 249 974 2352 6180 Fragments arranged by size: 6180 2352 974 249 PciI A'CATG_T Cuts at: 846 1025 6293 8907 846 Size: 179 5268 2614 1694 Fragments arranged by size: 5268 2614 1694 179 PflMI CCAn_nnn'nTGG Cuts at: 1356 1773 3987 4262 5198 1356 Size: 417 2214 275 936 5913 Fragments arranged by size: 5913 2214 936 417 275 PfoI T'CCnGG_A Cuts at: 1710 4718 5891 1710 Size: 3008 1173 5574 Fragments arranged by size: 5574 3008 1173 PmeI GTTT'AAAC Cuts at: 6754 6754 Size: 9755 PmlI CAC'GTG Cuts at: 4361 4361 Size: 9755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2368 2400 8201 8233 2368 Size: 32 5801 32 3890 Fragments arranged by size: 5801 3890 32 32 PpuMI rG'GwC_Cy Cuts at: 779 3080 3270 4394 5042 779 Size: 2301 190 1124 648 5492 Fragments arranged by size: 5492 2301 1124 648 190 PspOMI G'GGCC_C Cuts at: 4351 4351 Size: 9755 PstI C_TGCA'G Cuts at: 365 3229 3364 3604 3658 4387 4756 5679 6605 Size: 2864 135 240 54 729 369 923 926 Cuts at: 6605 6802 7487 365 Size: 197 685 2633 Fragments arranged by size: 2864 2633 926 923 729 685 369 240 197 135 54 PvuI CG_AT'CG Cuts at: 104 104 Size: 9755 PvuII CAG'CTG Cuts at: 727 798 1934 2640 4801 5082 7180 7540 727 Size: 71 1136 706 2161 281 2098 360 2942 Fragments arranged by size: 2942 2161 2098 1136 706 360 281 71 RsrII CG'GwC_CG Cuts at: 7950 7950 Size: 9755 SacI G_AGCT'C Cuts at: 186 6769 186 Size: 6583 3172 SalI G'TCGA_C Cuts at: 6792 6792 Size: 9755 SanDI GG'GwC_CC Cuts at: 3080 3080 Size: 9755 SapI GCTCTTCn'nnn_ Cuts at: 1879 5758 7778 7988 1879 Size: 3879 2020 210 3646 Fragments arranged by size: 3879 3646 2020 210 SbfI CC_TGCA'GG Cuts at: 6802 6802 Size: 9755 ScaI AGT'ACT Cuts at: 9272 9272 Size: 9755 SexAI A'CCwGG_T Cuts at: 1178 2849 1178 Size: 1671 8084 SfcI C'TryA_G Cuts at: 33 361 3225 3360 3600 3654 4383 4752 4881 Size: 328 2864 135 240 54 729 369 129 Cuts at: 4881 5675 6601 6636 6798 7483 8451 8642 33 Size: 794 926 35 162 685 968 191 1146 Fragments arranged by size: 2864 1146 968 926 794 729 685 369 328 240 191 162 135 129 54 35 SfoI GGC'GCC Cuts at: 7187 7435 7187 Size: 248 9507 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 9755 SmaI CCC'GGG Cuts at: 967 2143 6777 967 Size: 1176 4634 3945 Fragments arranged by size: 4634 3945 1176 SmlI C'TyrA_G Cuts at: 988 3304 3526 5650 6910 7825 8539 8801 988 Size: 2316 222 2124 1260 915 714 262 1942 Fragments arranged by size: 2316 2124 1942 1260 915 714 262 222 SpeI A'CTAG_T Cuts at: 67 5287 67 Size: 5220 4535 SphI G_CATG'C Cuts at: 6808 7308 6808 Size: 500 9255 SspI AAT'ATT Cuts at: 1153 1153 Size: 9755 StuI AGG'CCT Cuts at: 751 1613 3709 4684 4750 751 Size: 862 2096 975 66 5756 Fragments arranged by size: 5756 2096 975 862 66 StyI C'CwwG_G Cuts at: 107 464 1262 1310 1595 1967 2531 3203 3986 Size: 357 798 48 285 372 564 672 783 Cuts at: 3986 5145 5411 6327 6737 6889 7243 107 Size: 1159 266 916 410 152 354 2619 Fragments arranged by size: 2619 1159 916 798 783 672 564 410 372 357 354 285 266 152 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1416 3484 4404 4519 4538 5158 5485 7338 7471 Size: 2068 920 115 19 620 327 1853 133 Cuts at: 7471 7930 8139 1416 Size: 459 209 3032 Fragments arranged by size: 3032 2068 1853 920 620 459 327 209 133 115 19 TatI w'GTAC_w Cuts at: 946 1022 1667 3593 4772 4779 4986 5162 6581 Size: 76 645 1926 1179 7 207 176 1419 Cuts at: 6581 9270 946 Size: 2689 1431 Fragments arranged by size: 2689 1926 1431 1419 1179 645 207 176 76 7 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1122 1154 2140 2172 2854 2884 2886 2916 3262 Size: 32 986 32 682 30 2 30 346 Cuts at: 3262 3294 3937 3969 4563 4595 4651 4683 5348 Size: 32 643 32 594 32 56 32 665 Cuts at: 5348 5380 6625 6657 9272 9304 1122 Size: 32 1245 32 2615 32 1573 Fragments arranged by size: 2615 1573 1245 986 682 665 643 594 346 56 32 32 32 32 32 32 32 32 32 30 30 2 Tth111I GACn'n_nGTC Cuts at: 7552 7552 Size: 9755 XbaI T'CTAG_A Cuts at: 6786 6786 Size: 9755 XcmI CCAnnnn_n'nnnnTGG Cuts at: 762 1632 1965 3459 4105 5580 6369 7250 762 Size: 870 333 1494 646 1475 789 881 3267 Fragments arranged by size: 3267 1494 1475 881 870 789 646 333 XmaI C'CCGG_G Cuts at: 965 2141 6775 965 Size: 1176 4634 3945 Fragments arranged by size: 4634 3945 1176 XmnI GAAnn'nnTTC Cuts at: 1825 2383 3133 5452 1825 Size: 558 750 2319 6128 Fragments arranged by size: 6128 2319 750 558 ZraI GAC'GTC Cuts at: 2538 6732 2538 Size: 4194 5561 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: AflII AgeI AhdI AloI AscI AvrII BsiWI BsmBI BstZ17I ClaI FseI FspAI HpaI NotI PacI PshAI PsiI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SwaI XhoI