Restriction map for
pF1KB4393
(Circular) (Six-base) MAPSORT of: pF1KB4393.seq Check: 9749 from: 1 to: 7283
pF1KB4393 7283 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3265 3265
Size: 7283
AccI GT'mk_AC
Cuts at: 2174 2684 4321 2174
Size: 510 1637 5136
Fragments arranged by size:
5136 1637 510
Acc65I G'GTAC_C
Cuts at: 598 4299 598
Size: 3701 3582
AclI AA'CG_TT
Cuts at: 7123 7123
Size: 7283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 502 673 824 1162 1548 1893 2257 2386
Size: 118 171 151 338 386 345 364 129
Cuts at: 2386 3277 5108 5540 6287 6555 384
Size: 891 1831 432 747 268 1112
Fragments arranged by size:
1831 1112 891 747 432 386 364 345
338 268 171 151 129 118
AflIII A'CryG_T
Cuts at: 4635 5739 6716 4635
Size: 1104 977 5202
Fragments arranged by size:
5202 1104 977
AgeI A'CCGG_T
Cuts at: 5671 5671
Size: 7283
AleI CACnn'nnGTG
Cuts at: 3931 3931
Size: 7283
AlwNI CAG_nnn'CTG
Cuts at: 483 2405 3199 3681 4588 6155 483
Size: 1922 794 482 907 1567 1611
Fragments arranged by size:
1922 1611 1567 907 794 482
ApaLI G'TGCA_C
Cuts at: 6053 6053
Size: 7283
ApoI r'AATT_y
Cuts at: 681 780 1271 3019 4255 4287 6636 6892 681
Size: 99 491 1748 1236 32 2349 256 1072
Fragments arranged by size:
2349 1748 1236 1072 491 256 99 32
AseI AT'TA_AT
Cuts at: 20 20
Size: 7283
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7283
AvaI C'yCGr_G
Cuts at: 1083 1701 2528 4303 1083
Size: 618 827 1775 4063
Fragments arranged by size:
4063 1775 827 618
AvrII C'CTAG_G
Cuts at: 2070 2070
Size: 7283
BamHI G'GATC_C
Cuts at: 666 2080 4308 666
Size: 1414 2228 3641
Fragments arranged by size:
3641 2228 1414
BanI G'GyrC_C
Cuts at: 598 876 925 1104 1750 3241 3835 4299 4713
Size: 278 49 179 646 1491 594 464 414
Cuts at: 4713 4961 4996 598
Size: 248 35 2885
Fragments arranged by size:
2885 1491 646 594 464 414 278 248
179 49 35
BanII G_rGCy'C
Cuts at: 1082 1439 3098 3658 4184 4297 5327 1082
Size: 357 1659 560 526 113 1030 3038
Fragments arranged by size:
3038 1659 1030 560 526 357 113
BbeI G_GCGC'C
Cuts at: 4717 4965 4717
Size: 248 7035
BbsI GAAGACnn'nnnn_
Cuts at: 2892 3265 4081 2892
Size: 373 816 6094
Fragments arranged by size:
6094 816 373
BbvCI CC'TCA_GC
Cuts at: 2339 2339
Size: 7283
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3933 3967 3933
Size: 34 7249
BciVI GTATCCnnnnn_n'
Cuts at: 2707 5181 5942 7283 2707
Size: 2474 761 1341 2707
Fragments arranged by size:
2707 2474 1341 761
BclI T'GATC_A
Cuts at: 1022 4803 1022
Size: 3781 3502
BglI GCCn_nnn'nGGC
Cuts at: 2164 2164
Size: 7283
BglII A'GATC_T
Cuts at: 1904 1995 2748 4798 6835 1904
Size: 91 753 2050 2037 2352
Fragments arranged by size:
2352 2050 2037 753 91
BlpI GC'TnA_GC
Cuts at: 4395 4395
Size: 7283
Bme1580I G_kGCm'C
Cuts at: 750 1062 3183 3222 3311 3838 4908 5001 6057
Size: 312 2121 39 89 527 1070 93 1056
Cuts at: 6057 750
Size: 1976
Fragments arranged by size:
2121 1976 1070 1056 527 312 93 89
39
BmrI ACTGGGnnnn_n'
Cuts at: 1044 2127 2754 2906 4495 4576 4911 7055 1044
Size: 1083 627 152 1589 81 335 2144 1272
Fragments arranged by size:
2144 1589 1272 1083 627 335 152 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1189 5650 1189
Size: 4461 2822
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 2371 3172 391
Size: 1980 801 4502
Fragments arranged by size:
4502 1980 801
Bpu10I CC'TnA_GC
Cuts at: 2339 2339
Size: 7283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1338 1415 1423 1753 4459 5338 5830 6128 1338
Size: 77 8 330 2706 879 492 298 2493
Fragments arranged by size:
2706 2493 879 492 330 298 77 8
BsaI GGTCTCn'nnnn_
Cuts at: 2338 3039 3148 6987 2338
Size: 701 109 3839 2634
Fragments arranged by size:
3839 2634 701 109
BsaAI yAC'GTr
Cuts at: 3669 3966 4636 3669
Size: 297 670 6316
Fragments arranged by size:
6316 670 297
BsaBI GATnn'nnATC
Cuts at: 6 1937 4797 6834 6
Size: 1931 2860 2037 455
Fragments arranged by size:
2860 2037 1931 455
BsaHI Gr'CG_yC
Cuts at: 4714 4962 5664 7160 4714
Size: 248 702 1496 4837
Fragments arranged by size:
4837 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1232 1556 2626 4472 4993 5671 5945 6092 6503
Size: 324 1070 1846 521 678 274 147 411
Cuts at: 6503 1232
Size: 2012
Fragments arranged by size:
2012 1846 1070 678 521 411 324 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 364 394 1469 1499 364
Size: 30 1075 30 6148
Fragments arranged by size:
6148 1075 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 481 1750 2356 2831 199
Size: 282 1269 606 475 4651
Fragments arranged by size:
4651 1269 606 475 282
BseYI C'CCAG_C
Cuts at: 738 798 2589 4708 5466 6043 6539 738
Size: 60 1791 2119 758 577 496 1482
Fragments arranged by size:
2119 1791 1482 758 577 496 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1495 2316 3552 3730 1495
Size: 821 1236 178 5048
Fragments arranged by size:
5048 1236 821 178
BsiEI CG_ry'CG
Cuts at: 104 2670 4825 4871 6079 104
Size: 2566 2155 46 1208 1308
Fragments arranged by size:
2566 2155 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 275 1082 1439 3213 3658 4297 5075 5265 6057
Size: 807 357 1774 445 639 778 190 792
Cuts at: 6057 275
Size: 1501
Fragments arranged by size:
1774 1501 807 792 778 639 445 357
190
BsmI GAATG_Cn'
Cuts at: 1088 2265 3716 1088
Size: 1177 1451 4655
Fragments arranged by size:
4655 1451 1177
BsmBI CGTCTCn'nnnn_
Cuts at: 124 124
Size: 7283
Bsp1286I G_dGCh'C
Cuts at: 275 750 1062 1082 1439 3098 3183 3213 3222
Size: 475 312 20 357 1659 85 30 9
Cuts at: 3222 3311 3658 3838 4184 4297 4908 5001 5075
Size: 89 347 180 346 113 611 93 74
Cuts at: 5075 5265 5327 6057 275
Size: 190 62 730 1501
Fragments arranged by size:
1659 1501 730 611 475 357 347 346
312 190 180 113 93 89 85 74
62 30 20 9
BspEI T'CCGG_A
Cuts at: 1232 6503 1232
Size: 5271 2012
BspHI T'CATG_A
Cuts at: 633 2963 3986 6459 633
Size: 2330 1023 2473 1457
Fragments arranged by size:
2473 2330 1457 1023
BspMI ACCTGCnnnn'nnnn_
Cuts at: 448 1964 3265 3809 4333 4547 4849 5230 448
Size: 1516 1301 544 524 214 302 381 2501
Fragments arranged by size:
2501 1516 1301 544 524 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5575 5629 7113 7278 50
Size: 5525 54 1484 165 55
Fragments arranged by size:
5525 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1808 5195 1808
Size: 3387 3896
BsrFI r'CCGG_y
Cuts at: 2263 2665 5281 5462 5671 2263
Size: 402 2616 181 209 3875
Fragments arranged by size:
3875 2616 402 209 181
BsrGI T'GTAC_A
Cuts at: 230 1585 230
Size: 1355 5928
BssSI C'ACGA_G
Cuts at: 3406 5554 5912 3406
Size: 2148 358 4777
Fragments arranged by size:
4777 2148 358
BstAPI GCAn_nnn'nTGC
Cuts at: 440 2405 3208 3969 4660 440
Size: 1965 803 761 691 3063
Fragments arranged by size:
3063 1965 803 761 691
BstBI TT'CG_AA
Cuts at: 5644 5644
Size: 7283
BstXI CCAn_nnnn'nTGG
Cuts at: 583 3229 3705 583
Size: 2646 476 4161
Fragments arranged by size:
4161 2646 476
BstYI r'GATC_y
Cuts at: 1 666 998 1904 1995 2080 2748 2829 3690
Size: 665 332 906 91 85 668 81 861
Cuts at: 3690 4308 4775 4798 5133 5379 6391 6477 6489
Size: 618 467 23 335 246 1012 86 12
Cuts at: 6489 6835 1
Size: 346 449
Fragments arranged by size:
1012 906 861 668 665 618 467 449
346 335 332 246 91 86 85 81
23 12
Bsu36I CC'TnA_GG
Cuts at: 182 1343 1921 182
Size: 1161 578 5544
Fragments arranged by size:
5544 1161 578
BtgI C'CryG_G
Cuts at: 107 2187 2444 3929 3939 107
Size: 2080 257 1485 10 3451
Fragments arranged by size:
3451 2080 1485 257 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1946 5233 5414 5577 1946
Size: 3287 181 163 3652
Fragments arranged by size:
3652 3287 181 163
BtsI GCAGTG_nn'
Cuts at: 1060 2333 2406 3209 4526 1060
Size: 1273 73 803 1317 3817
Fragments arranged by size:
3817 1317 1273 803 73
ClaI AT'CG_AT
Cuts at: 1472 1472
Size: 7283
DraI TTT'AAA
Cuts at: 789 2871 4282 789
Size: 2082 1411 3790
Fragments arranged by size:
3790 2082 1411
DraIII CAC_nnn'GTG
Cuts at: 1889 3826 3828 1889
Size: 1937 2 5344
Fragments arranged by size:
5344 1937 2
DrdI GACnn_nn'nnGTC
Cuts at: 2955 4989 5847 2955
Size: 2034 858 4391
Fragments arranged by size:
4391 2034 858
EaeI y'GGCC_r
Cuts at: 140 1265 1538 2223 2663 2667 3815 4031 4868
Size: 1125 273 685 440 4 1148 216 837
Cuts at: 4868 5042 5433 5460 6805 140
Size: 174 391 27 1345 618
Fragments arranged by size:
1345 1148 1125 837 685 618 440 391
273 216 174 27 4
EagI C'GGCC_G
Cuts at: 2667 4868 2667
Size: 2201 5082
EarI CTCTTCn'nnn_
Cuts at: 2965 4189 5306 5516 2965
Size: 1224 1117 210 4732
Fragments arranged by size:
4732 1224 1117 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5491 5801 5947 7093 5491
Size: 310 146 1146 5681
Fragments arranged by size:
5681 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1080 1437 3656 4295 1080
Size: 357 2219 639 4068
Fragments arranged by size:
4068 2219 639 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 391 502 673 824 1162 1548 1893 2257
Size: 7 111 171 151 338 386 345 364
Cuts at: 2257 2371 2386 3172 3277 5108 5540 6287 6555
Size: 114 15 786 105 1831 432 747 268
Cuts at: 6555 384
Size: 1112
Fragments arranged by size:
1831 1112 786 747 432 386 364 345
338 268 171 151 114 111 105 15
7
EcoNI CCTnn'n_nnAGG
Cuts at: 2225 3685 2225
Size: 1460 5823
EcoO109I rG'GnC_Cy
Cuts at: 1662 2555 4212 4232 4422 1662
Size: 893 1657 20 190 4523
Fragments arranged by size:
4523 1657 893 190 20
EcoRI G'AATT_C
Cuts at: 4255 4287 4255
Size: 32 7251
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1708 1923 3487 5302 1708
Size: 215 1564 1815 3689
Fragments arranged by size:
3689 1815 1564 215
FseI GG_CCGG'CC
Cuts at: 2669 2669
Size: 7283
FspI TGC'GCA
Cuts at: 5064 5064
Size: 7283
HaeII r_GCGC'y
Cuts at: 590 4717 4965 5987 6966 590
Size: 4127 248 1022 979 907
Fragments arranged by size:
4127 1022 979 907 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 904 936 1115 1147 1454 1486 2131 2163 2740
Size: 32 179 32 307 32 645 32 577
Cuts at: 2740 2772 3548 3580 3725 3757 5125 5155 5157
Size: 32 776 32 145 32 1368 30 2
Cuts at: 5157 5187 904
Size: 30 3000
Fragments arranged by size:
3000 1368 776 645 577 307 179 145
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2685 4322 2685
Size: 1637 5646
HindIII A'AGCT_T
Cuts at: 3778 3778
Size: 7283
KasI G'GCGC_C
Cuts at: 4713 4961 4713
Size: 248 7035
KpnI G_GTAC'C
Cuts at: 602 4303 602
Size: 3701 3582
MfeI C'AATT_G
Cuts at: 2198 2198
Size: 7283
MluI A'CGCG_T
Cuts at: 6716 6716
Size: 7283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1158 4004 5954 6138 1158
Size: 2846 1950 184 2303
Fragments arranged by size:
2846 2303 1950 184
MscI TGG'CCA
Cuts at: 142 1267 1540 2225 3817 4033 5044 142
Size: 1125 273 685 1592 216 1011 2381
Fragments arranged by size:
2381 1592 1125 1011 685 273 216
MslI CAynn'nnrTG
Cuts at: 581 3247 3931 4150 5272 5399 581
Size: 2666 684 219 1122 127 2465
Fragments arranged by size:
2666 2465 1122 684 219 127
MspA1I CmG'CkG
Cuts at: 802 1050 1719 2593 2704 3114 4394 4708 5068
Size: 248 669 874 111 410 1280 314 360
Cuts at: 5068 6081 6326 802
Size: 1013 245 1759
Fragments arranged by size:
1759 1280 1013 874 669 410 360 314
248 245 111
NaeI GCC'GGC
Cuts at: 2265 2667 5464 2265
Size: 402 2797 4084
Fragments arranged by size:
4084 2797 402
NarI GG'CG_CC
Cuts at: 4714 4962 4714
Size: 248 7035
NcoI C'CATG_G
Cuts at: 107 2444 3929 107
Size: 2337 1485 3461
Fragments arranged by size:
3461 2337 1485
NgoMIV G'CCGG_C
Cuts at: 2263 2665 5462 2263
Size: 402 2797 4084
Fragments arranged by size:
4084 2797 402
NspI r_CATG'y
Cuts at: 253 2721 4336 4836 5743 253
Size: 2468 1615 500 907 1793
Fragments arranged by size:
2468 1793 1615 907 500
PasI CC'CwG_GG
Cuts at: 3300 3300
Size: 7283
PciI A'CATG_T
Cuts at: 5739 5739
Size: 7283
PflMI CCAn_nnn'nTGG
Cuts at: 285 1115 2661 285
Size: 830 1546 4907
Fragments arranged by size:
4907 1546 830
PfoI T'CCnGG_A
Cuts at: 125 2411 125
Size: 2286 4997
PmeI GTTT'AAAC
Cuts at: 4282 4282
Size: 7283
PmlI CAC'GTG
Cuts at: 3966 3966
Size: 7283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6422 6454 6422
Size: 32 7251
PpuMI rG'GwC_Cy
Cuts at: 2555 4212 2555
Size: 1657 5626
PstI C_TGCA'G
Cuts at: 1049 1055 2139 2318 2838 3008 3204 3677 4330
Size: 6 1084 179 520 170 196 473 653
Cuts at: 4330 5015 1049
Size: 685 3317
Fragments arranged by size:
3317 1084 685 653 520 473 196 179
170 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7283
PvuII CAG'CTG
Cuts at: 802 1050 2593 2704 3114 4708 5068 802
Size: 248 1543 111 410 1594 360 3017
Fragments arranged by size:
3017 1594 1543 410 360 248 111
RsrII CG'GwC_CG
Cuts at: 5478 5478
Size: 7283
SacI G_AGCT'C
Cuts at: 1082 1439 3658 4297 1082
Size: 357 2219 639 4068
Fragments arranged by size:
4068 2219 639 357
SalI G'TCGA_C
Cuts at: 2683 4320 2683
Size: 1637 5646
SanDI GG'GwC_CC
Cuts at: 4212 4212
Size: 7283
SapI GCTCTTCn'nnn_
Cuts at: 4189 5306 5516 4189
Size: 1117 210 5956
Fragments arranged by size:
5956 1117 210
SbfI CC_TGCA'GG
Cuts at: 3008 4330 3008
Size: 1322 5961
ScaI AGT'ACT
Cuts at: 1978 6800 1978
Size: 4822 2461
SfcI C'TryA_G
Cuts at: 33 1045 1051 2135 2314 2834 3004 3159 3200
Size: 1012 6 1084 179 520 170 155 41
Cuts at: 3200 3673 4326 5011 6004 6195 33
Size: 473 653 685 993 191 1121
Fragments arranged by size:
1121 1084 1012 993 685 653 520 473
191 179 170 155 41 6
SfoI GGC'GCC
Cuts at: 4715 4963 4715
Size: 248 7035
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7283
SmaI CCC'GGG
Cuts at: 4305 4305
Size: 7283
SmlI C'TyrA_G
Cuts at: 1317 1430 1438 1768 2528 4438 5353 5845 6107
Size: 113 8 330 760 1910 915 492 262
Cuts at: 6107 1317
Size: 2493
Fragments arranged by size:
2493 1910 915 760 492 330 262 113
8
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7283
SphI G_CATG'C
Cuts at: 4336 4836 4336
Size: 500 6783
SspI AAT'ATT
Cuts at: 2094 2094
Size: 7283
StuI AGG'CCT
Cuts at: 1926 1926
Size: 7283
StyI C'CwwG_G
Cuts at: 107 1793 1964 2070 2226 2444 3338 3698 3929
Size: 1686 171 106 156 218 894 360 231
Cuts at: 3929 4417 4771 107
Size: 488 354 2619
Fragments arranged by size:
2619 1686 894 488 360 354 231 218
171 156 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1122 1367 3148 3196 3259 4866 4999 5458 5667
Size: 245 1781 48 63 1607 133 459 209
Cuts at: 5667 1122
Size: 2738
Fragments arranged by size:
2738 1781 1607 459 245 209 133 63
48
TatI w'GTAC_w
Cuts at: 230 1585 1976 2285 3170 3662 3773 3923 6798
Size: 1355 391 309 885 492 111 150 2875
Cuts at: 6798 230
Size: 715
Fragments arranged by size:
2875 1355 885 715 492 391 309 150
111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 364 396 1172 1204 1469 1501 4111 4143 6800
Size: 32 776 32 265 32 2610 32 2657
Cuts at: 6800 6832 364
Size: 32 815
Fragments arranged by size:
2657 2610 815 776 265 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 5080 5080
Size: 7283
XbaI T'CTAG_A
Cuts at: 4314 4314
Size: 7283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 163 657 2604 2809 4778 163
Size: 494 1947 205 1969 2668
Fragments arranged by size:
2668 1969 1947 494 205
XhoI C'TCGA_G
Cuts at: 2528 2528
Size: 7283
XmaI C'CCGG_G
Cuts at: 4303 4303
Size: 7283
XmnI GAAnn'nnTTC
Cuts at: 809 3146 3262 3467 809
Size: 2337 116 205 4625
Fragments arranged by size:
4625 2337 205 116
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
BaeI BfrBI BmgBI BmtI BsiWI BssHII BstEII BstZ17I
CspCI EcoRV FspAI HpaI NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SwaI ZraI