Restriction map for
pF1KB4392
(Circular) (Six-base) MAPSORT of: pF1KB4392.seq Check: 6841 from: 1 to: 4181
pF1KB4392 4181 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 824 824
Size: 4181
AccI GT'mk_AC
Cuts at: 1219 1219
Size: 4181
Acc65I G'GTAC_C
Cuts at: 1197 1197
Size: 4181
AclI AA'CG_TT
Cuts at: 4021 4021
Size: 4181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 2006 2438 3185 3453 680
Size: 1326 432 747 268 1408
Fragments arranged by size:
1408 1326 747 432 268
AfeI AGC'GCT
Cuts at: 267 267
Size: 4181
AflIII A'CryG_T
Cuts at: 1533 2637 3614 1533
Size: 1104 977 2100
Fragments arranged by size:
2100 1104 977
AgeI A'CCGG_T
Cuts at: 731 2569 731
Size: 1838 2343
AleI CACnn'nnGTG
Cuts at: 549 549
Size: 4181
AlwNI CAG_nnn'CTG
Cuts at: 145 1486 3053 145
Size: 1341 1567 1273
Fragments arranged by size:
1567 1341 1273
ApaLI G'TGCA_C
Cuts at: 2951 2951
Size: 4181
ApoI r'AATT_y
Cuts at: 1185 3534 3790 1185
Size: 2349 256 1576
Fragments arranged by size:
2349 1576 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4181
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
AvaI C'yCGr_G
Cuts at: 190 227 521 560 879 935 1019 1049 1079
Size: 37 294 39 319 56 84 30 30
Cuts at: 1079 1168 1201 190
Size: 89 33 3170
Fragments arranged by size:
3170 319 294 89 84 56 39 37
33 30 30
BamHI G'GATC_C
Cuts at: 1206 1206
Size: 4181
BanI G'GyrC_C
Cuts at: 339 864 1197 1611 1859 1894 339
Size: 525 333 414 248 35 2626
Fragments arranged by size:
2626 525 414 333 248 35
BanII G_rGCy'C
Cuts at: 724 1195 2225 724
Size: 471 1030 2680
Fragments arranged by size:
2680 1030 471
BbeI G_GCGC'C
Cuts at: 1615 1863 1615
Size: 248 3933
BbsI GAAGACnn'nnnn_
Cuts at: 384 384
Size: 4181
BbvCI CC'TCA_GC
Cuts at: 407 839 898 407
Size: 432 59 3690
Fragments arranged by size:
3690 432 59
BciVI GTATCCnnnnn_n'
Cuts at: 422 1038 2079 2840 4181 422
Size: 616 1041 761 1341 422
Fragments arranged by size:
1341 1041 761 616 422
BclI T'GATC_A
Cuts at: 1701 1701
Size: 4181
BglII A'GATC_T
Cuts at: 283 1696 3733 283
Size: 1413 2037 731
Fragments arranged by size:
2037 1413 731
BlpI GC'TnA_GC
Cuts at: 650 1293 650
Size: 643 3538
Bme1580I G_kGCm'C
Cuts at: 869 1806 1899 2955 869
Size: 937 93 1056 2095
Fragments arranged by size:
2095 1056 937 93
BmrI ACTGGGnnnn_n'
Cuts at: 1393 1474 1809 3953 1393
Size: 81 335 2144 1621
Fragments arranged by size:
2144 1621 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 284 1094 2548 284
Size: 810 1454 1917
Fragments arranged by size:
1917 1454 810
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 858 1119 736
Size: 122 261 3798
Fragments arranged by size:
3798 261 122
Bpu10I CC'TnA_GC
Cuts at: 407 839 898 407
Size: 432 59 3690
Fragments arranged by size:
3690 432 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 958 1357 2236 2728 3026 421
Size: 537 399 879 492 298 1576
Fragments arranged by size:
1576 879 537 492 399 298
BsaI GGTCTCn'nnnn_
Cuts at: 740 974 1065 3885 740
Size: 234 91 2820 1036
Fragments arranged by size:
2820 1036 234 91
BsaAI yAC'GTr
Cuts at: 1534 1534
Size: 4181
BsaBI GATnn'nnATC
Cuts at: 6 1695 3732 6
Size: 1689 2037 455
Fragments arranged by size:
2037 1689 455
BsaHI Gr'CG_yC
Cuts at: 1612 1860 2562 4058 1612
Size: 248 702 1496 1735
Fragments arranged by size:
1735 1496 702 248
BsaWI w'CCGG_w
Cuts at: 425 731 1370 1891 2569 2843 2990 3401 425
Size: 306 639 521 678 274 147 411 1205
Fragments arranged by size:
1205 678 639 521 411 306 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 512 1120 1135 512
Size: 608 15 3558
Fragments arranged by size:
3558 608 15
BseYI C'CCAG_C
Cuts at: 138 151 502 908 927 1013 1035 1060 1606
Size: 13 351 406 19 86 22 25 546
Cuts at: 1606 2364 2941 3437 138
Size: 758 577 496 882
Fragments arranged by size:
882 758 577 546 496 406 351 86
25 22 19 13
BsiEI CG_ry'CG
Cuts at: 104 1723 1769 2977 104
Size: 1619 46 1208 1308
Fragments arranged by size:
1619 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1195 1973 2163 2955 1195
Size: 778 190 792 2421
Fragments arranged by size:
2421 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 724 869 1195 1806 1899 1973 2163 2225 2955
Size: 145 326 611 93 74 190 62 730
Cuts at: 2955 724
Size: 1950
Fragments arranged by size:
1950 730 611 326 190 145 93 74
62
BspEI T'CCGG_A
Cuts at: 3401 3401
Size: 4181
BspHI T'CATG_A
Cuts at: 3357 3357
Size: 4181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 306 464 824 1231 1445 1747 2128 306
Size: 158 360 407 214 302 381 2359
Fragments arranged by size:
2359 407 381 360 302 214 158
BsrBI CCG'CTC
Cuts at: 50 565 1143 2473 2527 4011 4176 50
Size: 515 578 1330 54 1484 165 55
Fragments arranged by size:
1484 1330 578 515 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2093 2093
Size: 4181
BsrFI r'CCGG_y
Cuts at: 731 2179 2360 2569 731
Size: 1448 181 209 2343
Fragments arranged by size:
2343 1448 209 181
BssSI C'ACGA_G
Cuts at: 2452 2810 2452
Size: 358 3823
BstAPI GCAn_nnn'nTGC
Cuts at: 1558 1558
Size: 4181
BstBI TT'CG_AA
Cuts at: 2542 2542
Size: 4181
BstYI r'GATC_y
Cuts at: 1 283 394 1146 1206 1673 1696 2031 2277
Size: 282 111 752 60 467 23 335 246
Cuts at: 2277 3289 3375 3387 3733 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 752 467 449 346 335 282 246
111 86 60 23 12
Bsu36I CC'TnA_GG
Cuts at: 677 677
Size: 4181
BtgI C'CryG_G
Cuts at: 600 705 600
Size: 105 4076
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2131 2312 2475 2131
Size: 181 163 3837
Fragments arranged by size:
3837 181 163
BtsI GCAGTG_nn'
Cuts at: 362 806 1424 362
Size: 444 618 3119
Fragments arranged by size:
3119 618 444
DraI TTT'AAA
Cuts at: 1180 1180
Size: 4181
DrdI GACnn_nn'nnGTC
Cuts at: 1887 2745 1887
Size: 858 3323
EaeI y'GGCC_r
Cuts at: 1085 1766 1940 2331 2358 3703 1085
Size: 681 174 391 27 1345 1563
Fragments arranged by size:
1563 1345 681 391 174 27
EagI C'GGCC_G
Cuts at: 1766 1766
Size: 4181
EarI CTCTTCn'nnn_
Cuts at: 2204 2414 2204
Size: 210 3971
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2389 2699 2845 3991 2389
Size: 310 146 1146 2579
Fragments arranged by size:
2579 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1193 1193
Size: 4181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 736 858 1119 2006 2438 3185 3453 680
Size: 56 122 261 887 432 747 268 1408
Fragments arranged by size:
1408 887 747 432 268 261 122 56
EcoNI CCTnn'n_nnAGG
Cuts at: 163 163
Size: 4181
EcoO109I rG'GnC_Cy
Cuts at: 243 453 835 931 1320 243
Size: 210 382 96 389 3104
Fragments arranged by size:
3104 389 382 210 96
EcoRI G'AATT_C
Cuts at: 1185 1185
Size: 4181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 4181
FspI TGC'GCA
Cuts at: 1962 1962
Size: 4181
HaeII r_GCGC'y
Cuts at: 269 1615 1863 2885 3864 269
Size: 1346 248 1022 979 586
Fragments arranged by size:
1346 1022 979 586 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2023 2053 2055 2085 2023
Size: 30 2 30 4119
Fragments arranged by size:
4119 30 30 2
HincII GTy'rAC
Cuts at: 1220 1220
Size: 4181
KasI G'GCGC_C
Cuts at: 1611 1859 1611
Size: 248 3933
KpnI G_GTAC'C
Cuts at: 1201 1201
Size: 4181
MluI A'CGCG_T
Cuts at: 3614 3614
Size: 4181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 815 2852 3036 815
Size: 2037 184 1960
Fragments arranged by size:
2037 1960 184
MscI TGG'CCA
Cuts at: 1087 1942 1087
Size: 855 3326
MslI CAynn'nnrTG
Cuts at: 549 2170 2297 549
Size: 1621 127 2433
Fragments arranged by size:
2433 1621 127
MspA1I CmG'CkG
Cuts at: 580 1292 1606 1966 2979 3224 580
Size: 712 314 360 1013 245 1537
Fragments arranged by size:
1537 1013 712 360 314 245
NaeI GCC'GGC
Cuts at: 2362 2362
Size: 4181
NarI GG'CG_CC
Cuts at: 1612 1860 1612
Size: 248 3933
NcoI C'CATG_G
Cuts at: 705 705
Size: 4181
NgoMIV G'CCGG_C
Cuts at: 2360 2360
Size: 4181
NspI r_CATG'y
Cuts at: 1234 1734 2641 1234
Size: 500 907 2774
Fragments arranged by size:
2774 907 500
PciI A'CATG_T
Cuts at: 2637 2637
Size: 4181
PmeI GTTT'AAAC
Cuts at: 1180 1180
Size: 4181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3320 3352 3320
Size: 32 4149
PpuMI rG'GwC_Cy
Cuts at: 453 835 931 453
Size: 382 96 3703
Fragments arranged by size:
3703 382 96
PspXI vC'TCGA_Gb
Cuts at: 560 1168 560
Size: 608 3573
PstI C_TGCA'G
Cuts at: 225 1228 1913 225
Size: 1003 685 2493
Fragments arranged by size:
2493 1003 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4181
PvuII CAG'CTG
Cuts at: 580 1606 1966 580
Size: 1026 360 2795
Fragments arranged by size:
2795 1026 360
RsrII CG'GwC_CG
Cuts at: 2376 2376
Size: 4181
SacI G_AGCT'C
Cuts at: 1195 1195
Size: 4181
SalI G'TCGA_C
Cuts at: 1218 1218
Size: 4181
SanDI GG'GwC_CC
Cuts at: 931 931
Size: 4181
SapI GCTCTTCn'nnn_
Cuts at: 2204 2414 2204
Size: 210 3971
SbfI CC_TGCA'GG
Cuts at: 1228 1228
Size: 4181
ScaI AGT'ACT
Cuts at: 757 3698 757
Size: 2941 1240
SfcI C'TryA_G
Cuts at: 33 221 999 1224 1909 2902 3093 33
Size: 188 778 225 685 993 191 1121
Fragments arranged by size:
1121 993 778 685 225 191 188
SfoI GGC'GCC
Cuts at: 1613 1861 1613
Size: 248 3933
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
SmaI CCC'GGG
Cuts at: 523 881 937 1203 523
Size: 358 56 266 3501
Fragments arranged by size:
3501 358 266 56
SmlI C'TyrA_G
Cuts at: 400 560 973 1168 1336 2251 2743 3005 400
Size: 160 413 195 168 915 492 262 1576
Fragments arranged by size:
1576 915 492 413 262 195 168 160
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4181
SphI G_CATG'C
Cuts at: 1234 1734 1234
Size: 500 3681
SrfI GCCC'GGGC
Cuts at: 881 881
Size: 4181
StyI C'CwwG_G
Cuts at: 705 1315 1669 705
Size: 610 354 3217
Fragments arranged by size:
3217 610 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 603 790 1764 1897 2356 2565 603
Size: 187 974 133 459 209 2219
Fragments arranged by size:
2219 974 459 209 187 133
TatI w'GTAC_w
Cuts at: 755 3696 755
Size: 2941 1240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 787 819 3698 3730 787
Size: 32 2879 32 1238
Fragments arranged by size:
2879 1238 32 32
Tth111I GACn'n_nGTC
Cuts at: 1978 1978
Size: 4181
XbaI T'CTAG_A
Cuts at: 1212 1212
Size: 4181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1676 1676
Size: 4181
XhoI C'TCGA_G
Cuts at: 560 1168 560
Size: 608 3573
XmaI C'CCGG_G
Cuts at: 521 879 935 1201 521
Size: 358 56 266 3501
Fragments arranged by size:
3501 358 266 56
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PsrI SacII
SexAI SfiI SgrAI SnaBI SspI StuI SwaI XmnI
ZraI