Restriction map for
pF1KB4380
(Circular) (Six-base) MAPSORT of: pF1KB4380.seq Check: 486 from: 1 to: 4286
pF1KB4380 4286 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1264 1264
Size: 4286
AccI GT'mk_AC
Cuts at: 1324 1324
Size: 4286
Acc65I G'GTAC_C
Cuts at: 1302 1302
Size: 4286
AclI AA'CG_TT
Cuts at: 4126 4126
Size: 4286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2111 2543 2896 3558 2111
Size: 432 353 662 2839
Fragments arranged by size:
2839 662 432 353
AfeI AGC'GCT
Cuts at: 898 1102 898
Size: 204 4082
AflIII A'CryG_T
Cuts at: 527 989 1638 3438 3719 527
Size: 462 649 1800 281 1094
Fragments arranged by size:
1800 1094 649 462 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1213 1245 1213
Size: 32 4254
AlwNI CAG_nnn'CTG
Cuts at: 1591 3029 1591
Size: 1438 2848
ApaI G_GGCC'C
Cuts at: 196 196
Size: 4286
ApaLI G'TGCA_C
Cuts at: 3124 3124
Size: 4286
ApoI r'AATT_y
Cuts at: 1290 3639 3895 1290
Size: 2349 256 1681
Fragments arranged by size:
2349 1681 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4286
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
AvaI C'yCGr_G
Cuts at: 159 196 1306 159
Size: 37 1110 3139
Fragments arranged by size:
3139 1110 37
BamHI G'GATC_C
Cuts at: 1311 1311
Size: 4286
BanI G'GyrC_C
Cuts at: 121 1252 1302 1716 1964 1999 121
Size: 1131 50 414 248 35 2408
Fragments arranged by size:
2408 1131 414 248 50 35
BanII G_rGCy'C
Cuts at: 196 752 1300 2330 196
Size: 556 548 1030 2152
Fragments arranged by size:
2152 1030 556 548
BbeI G_GCGC'C
Cuts at: 1720 1968 1720
Size: 248 4038
BbsI GAAGACnn'nnnn_
Cuts at: 382 681 1211 1233 382
Size: 299 530 22 3435
Fragments arranged by size:
3435 530 299 22
BbvCI CC'TCA_GC
Cuts at: 1126 1126
Size: 4286
BciVI GTATCCnnnnn_n'
Cuts at: 2184 3240 4286 2184
Size: 1056 1046 2184
Fragments arranged by size:
2184 1056 1046
BclI T'GATC_A
Cuts at: 1806 1806
Size: 4286
BglI GCCn_nnn'nGGC
Cuts at: 874 874
Size: 4286
BglII A'GATC_T
Cuts at: 1801 3838 1801
Size: 2037 2249
BlpI GC'TnA_GC
Cuts at: 433 508 559 998 1398 433
Size: 75 51 439 400 3321
Fragments arranged by size:
3321 439 400 75 51
Bme1580I G_kGCm'C
Cuts at: 196 443 1911 2004 3128 196
Size: 247 1468 93 1124 1354
Fragments arranged by size:
1468 1354 1124 247 93
BmgBI CAC'GTC
Cuts at: 1262 1262
Size: 4286
BmrI ACTGGGnnnn_n'
Cuts at: 1498 1579 1914 4058 1498
Size: 81 335 2144 1726
Fragments arranged by size:
2144 1726 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2653 2653
Size: 4286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1246 1246
Size: 4286
Bpu10I CC'TnA_GC
Cuts at: 1126 1126
Size: 4286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 856 1165 1462 2341 3055 3353 279
Size: 577 309 297 879 714 298 1212
Fragments arranged by size:
1212 879 714 577 309 298 297
BsaI GGTCTCn'nnnn_
Cuts at: 3990 3990
Size: 4286
BsaAI yAC'GTr
Cuts at: 351 576 837 972 1639 351
Size: 225 261 135 667 2998
Fragments arranged by size:
2998 667 261 225 135
BsaBI GATnn'nnATC
Cuts at: 6 1800 3837 6
Size: 1794 2037 455
Fragments arranged by size:
2037 1794 455
BsaHI Gr'CG_yC
Cuts at: 1010 1238 1717 1965 2667 4163 1010
Size: 228 479 248 702 1496 1133
Fragments arranged by size:
1496 1133 702 479 248 228
BsaWI w'CCGG_w
Cuts at: 1475 1996 2674 3085 3232 3506 1475
Size: 521 678 411 147 274 2255
Fragments arranged by size:
2255 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 120 132 238 1165 120
Size: 12 106 927 3241
Fragments arranged by size:
3241 927 106 12
BseYI C'CCAG_C
Cuts at: 154 1711 2469 3134 3542 154
Size: 1557 758 665 408 898
Fragments arranged by size:
1557 898 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 1242 997
Size: 245 4041
BsiEI CG_ry'CG
Cuts at: 104 1828 1874 3104 104
Size: 1724 46 1230 1286
Fragments arranged by size:
1724 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 509 560 842 1300 2078 2268 3128 509
Size: 51 282 458 778 190 860 1667
Fragments arranged by size:
1667 860 778 458 282 190 51
Bsp1286I G_dGCh'C
Cuts at: 196 443 509 560 752 842 1300 1911 2004
Size: 247 66 51 192 90 458 611 93
Cuts at: 2004 2078 2268 2330 3128 196
Size: 74 190 62 798 1354
Fragments arranged by size:
1354 798 611 458 247 192 190 93
90 74 66 62 51
BspHI T'CATG_A
Cuts at: 2718 2718
Size: 4286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1264 1336 1550 1852 2233 1264
Size: 72 214 302 381 3317
Fragments arranged by size:
3317 381 302 214 72
BsrBI CCG'CTC
Cuts at: 50 754 2578 2632 4116 4281 50
Size: 704 1824 54 1484 165 55
Fragments arranged by size:
1824 1484 704 165 55 54
BsrDI GCAATG_nn'
Cuts at: 410 845 2198 410
Size: 435 1353 2498
Fragments arranged by size:
2498 1353 435
BsrFI r'CCGG_y
Cuts at: 202 614 877 1155 2284 2465 202
Size: 412 263 278 1129 181 2023
Fragments arranged by size:
2023 1129 412 278 263 181
BsrGI T'GTAC_A
Cuts at: 782 782
Size: 4286
BssSI C'ACGA_G
Cuts at: 502 2557 3265 502
Size: 2055 708 1523
Fragments arranged by size:
2055 1523 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1121 1663 1121
Size: 542 3744
BstBI TT'CG_AA
Cuts at: 2647 2647
Size: 4286
BstYI r'GATC_y
Cuts at: 1 1311 1778 1801 2136 2382 2688 2700 2786
Size: 1310 467 23 335 246 306 12 86
Cuts at: 2786 3838 1
Size: 1052 449
Fragments arranged by size:
1310 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 305 581 623 701 812 1013 1145 305
Size: 276 42 78 111 201 132 3446
Fragments arranged by size:
3446 276 201 132 111 78 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2236 2417 2580 2236
Size: 181 163 3942
Fragments arranged by size:
3942 181 163
BtsI GCAGTG_nn'
Cuts at: 1529 1529
Size: 4286
DraI TTT'AAA
Cuts at: 1285 1285
Size: 4286
DrdI GACnn_nn'nnGTC
Cuts at: 1992 3336 1992
Size: 1344 2942
EaeI y'GGCC_r
Cuts at: 380 407 578 626 704 1871 2045 2436 2463
Size: 27 171 48 78 1167 174 391 27
Cuts at: 2463 3808 380
Size: 1345 858
Fragments arranged by size:
1345 1167 858 391 174 171 78 48
27 27
EagI C'GGCC_G
Cuts at: 1871 1871
Size: 4286
EarI CTCTTCn'nnn_
Cuts at: 2309 2519 2309
Size: 210 4076
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2494 3236 3382 4096 2494
Size: 742 146 714 2684
Fragments arranged by size:
2684 742 714 146
EcoICRI GAG'CTC
Cuts at: 1298 1298
Size: 4286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1246 2111 2543 2896 3558 1246
Size: 865 432 353 662 1974
Fragments arranged by size:
1974 865 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 192 193 254 1425 192
Size: 1 61 1171 3053
Fragments arranged by size:
3053 1171 61 1
EcoRI G'AATT_C
Cuts at: 1290 1290
Size: 4286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 933 2305 933
Size: 1372 2914
FspI TGC'GCA
Cuts at: 859 2067 859
Size: 1208 3078
FspAI rTGC'GCAy
Cuts at: 859 859
Size: 4286
HaeII r_GCGC'y
Cuts at: 209 281 900 1104 1720 1968 3198 3969 209
Size: 72 619 204 616 248 1230 771 526
Fragments arranged by size:
1230 771 619 616 526 248 204 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2128 2158 2160 2190 2128
Size: 30 2 30 4224
Fragments arranged by size:
4224 30 30 2
HincII GTy'rAC
Cuts at: 525 987 1161 1325 525
Size: 462 174 164 3486
Fragments arranged by size:
3486 462 174 164
KasI G'GCGC_C
Cuts at: 1716 1964 1716
Size: 248 4038
KpnI G_GTAC'C
Cuts at: 1306 1306
Size: 4286
MluI A'CGCG_T
Cuts at: 3719 3719
Size: 4286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1134 1236 3045 3229 1134
Size: 102 1809 184 2191
Fragments arranged by size:
2191 1809 184 102
MscI TGG'CCA
Cuts at: 409 628 2047 409
Size: 219 1419 2648
Fragments arranged by size:
2648 1419 219
MslI CAynn'nnrTG
Cuts at: 349 739 919 2275 2402 349
Size: 390 180 1356 127 2233
Fragments arranged by size:
2233 1356 390 180 127
MspA1I CmG'CkG
Cuts at: 167 703 981 1026 1055 1130 1147 1397 1711
Size: 536 278 45 29 75 17 250 314
Cuts at: 1711 2071 2855 3100 167
Size: 360 784 245 1353
Fragments arranged by size:
1353 784 536 360 314 278 250 245
75 45 29 17
NaeI GCC'GGC
Cuts at: 204 616 879 2467 204
Size: 412 263 1588 2023
Fragments arranged by size:
2023 1588 412 263
NarI GG'CG_CC
Cuts at: 1717 1965 1717
Size: 248 4038
NgoMIV G'CCGG_C
Cuts at: 202 614 877 2465 202
Size: 412 263 1588 2023
Fragments arranged by size:
2023 1588 412 263
NspI r_CATG'y
Cuts at: 531 738 1339 1839 3442 531
Size: 207 601 500 1603 1375
Fragments arranged by size:
1603 1375 601 500 207
PciI A'CATG_T
Cuts at: 527 3438 527
Size: 2911 1375
PfoI T'CCnGG_A
Cuts at: 1264 1264
Size: 4286
PmeI GTTT'AAAC
Cuts at: 1285 1285
Size: 4286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2732 2764 2732
Size: 32 4254
PpuMI rG'GwC_Cy
Cuts at: 254 254
Size: 4286
PspOMI G'GGCC_C
Cuts at: 192 192
Size: 4286
PstI C_TGCA'G
Cuts at: 1333 2018 1333
Size: 685 3601
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4286
PvuII CAG'CTG
Cuts at: 167 981 1026 1055 1130 1711 2071 167
Size: 814 45 29 75 581 360 2382
Fragments arranged by size:
2382 814 581 360 75 45 29
RsrII CG'GwC_CG
Cuts at: 2481 2481
Size: 4286
SacI G_AGCT'C
Cuts at: 1300 1300
Size: 4286
SacII CC_GC'GG
Cuts at: 704 1148 704
Size: 444 3842
SalI G'TCGA_C
Cuts at: 1323 1323
Size: 4286
SapI GCTCTTCn'nnn_
Cuts at: 2309 2519 2309
Size: 210 4076
SbfI CC_TGCA'GG
Cuts at: 1333 1333
Size: 4286
ScaI AGT'ACT
Cuts at: 3803 3803
Size: 4286
SfcI C'TryA_G
Cuts at: 33 1194 1329 2014 2982 3173 33
Size: 1161 135 685 968 191 1146
Fragments arranged by size:
1161 1146 968 685 191 135
SfiI GGCCn_nnn'nGGCC
Cuts at: 874 874
Size: 4286
SfoI GGC'GCC
Cuts at: 1718 1966 1718
Size: 248 4038
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
SmaI CCC'GGG
Cuts at: 161 198 1308 161
Size: 37 1110 3139
Fragments arranged by size:
3139 1110 37
SmlI C'TyrA_G
Cuts at: 258 871 1180 1441 2356 3070 3332 258
Size: 613 309 261 915 714 262 1212
Fragments arranged by size:
1212 915 714 613 309 262 261
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4286
SphI G_CATG'C
Cuts at: 1339 1839 1339
Size: 500 3786
SrfI GCCC'GGGC
Cuts at: 161 161
Size: 4286
StyI C'CwwG_G
Cuts at: 629 1420 1774 629
Size: 791 354 3141
Fragments arranged by size:
3141 791 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 642 1020 1869 2002 2461 2670 642
Size: 378 849 133 459 209 2258
Fragments arranged by size:
2258 849 459 378 209 133
TatI w'GTAC_w
Cuts at: 782 3801 782
Size: 3019 1267
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3803 3835 3803
Size: 32 4254
Tth111I GACn'n_nGTC
Cuts at: 520 2083 520
Size: 1563 2723
XbaI T'CTAG_A
Cuts at: 1317 1317
Size: 4286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1781 1781
Size: 4286
XmaI C'CCGG_G
Cuts at: 159 196 1306 159
Size: 37 1110 3139
Fragments arranged by size:
3139 1110 37
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AlfI AscI AvrII
BaeI BcgI BfrBI BmtI BsaXI BsiWI BsmI BsmBI
BspEI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspXI PsrI SanDI SexAI SgrAI
SnaBI SspI StuI SwaI XhoI XmnI ZraI