Restriction map for
pF1KB4367
(Circular) (Six-base) MAPSORT of: pF1KB4367.seq Check: 7074 from: 1 to: 6485
pF1KB4367 6485 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 3523 3523
Size: 6485
Acc65I G'GTAC_C
Cuts at: 3501 3501
Size: 6485
AclI AA'CG_TT
Cuts at: 164 6325 164
Size: 6161 324
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 557 1175 3395 3479 4310 4742 5489 5757
Size: 141 618 2220 84 831 432 747 268
Cuts at: 5757 416
Size: 1144
Fragments arranged by size:
2220 1144 831 747 618 432 268 141
84
AfeI AGC'GCT
Cuts at: 2591 3010 2591
Size: 419 6066
AflII C'TTAA_G
Cuts at: 882 2583 882
Size: 1701 4784
AflIII A'CryG_T
Cuts at: 795 3837 4941 5918 795
Size: 3042 1104 977 1362
Fragments arranged by size:
3042 1362 1104 977
AgeI A'CCGG_T
Cuts at: 4873 4873
Size: 6485
AleI CACnn'nnGTG
Cuts at: 2548 3140 2548
Size: 592 5893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3158 3158
Size: 6485
AlwNI CAG_nnn'CTG
Cuts at: 1804 1837 2530 3115 3790 5357 1804
Size: 33 693 585 675 1567 2932
Fragments arranged by size:
2932 1567 693 675 585 33
ApaLI G'TGCA_C
Cuts at: 2282 5255 2282
Size: 2973 3512
ApoI r'AATT_y
Cuts at: 195 823 887 1168 1578 3489 5838 6094 195
Size: 628 64 281 410 1911 2349 256 586
Fragments arranged by size:
2349 1911 628 586 410 281 256 64
AseI AT'TA_AT
Cuts at: 20 1730 20
Size: 1710 4775
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6485
AvaI C'yCGr_G
Cuts at: 3505 3505
Size: 6485
BamHI G'GATC_C
Cuts at: 3510 3510
Size: 6485
BanI G'GyrC_C
Cuts at: 531 3142 3501 3915 4163 4198 531
Size: 2611 359 414 248 35 2818
Fragments arranged by size:
2818 2611 414 359 248 35
BanII G_rGCy'C
Cuts at: 1940 3499 4529 1940
Size: 1559 1030 3896
Fragments arranged by size:
3896 1559 1030
BbeI G_GCGC'C
Cuts at: 3919 4167 3919
Size: 248 6237
BbsI GAAGACnn'nnnn_
Cuts at: 221 1483 1693 2021 2806 3215 221
Size: 1262 210 328 785 409 3491
Fragments arranged by size:
3491 1262 785 409 328 210
BbvCI CC'TCA_GC
Cuts at: 2276 2276
Size: 6485
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 433 467 433
Size: 34 6451
BciVI GTATCCnnnnn_n'
Cuts at: 4383 5144 6485 4383
Size: 761 1341 4383
Fragments arranged by size:
4383 1341 761
BclI T'GATC_A
Cuts at: 1503 2085 4005 1503
Size: 582 1920 3983
Fragments arranged by size:
3983 1920 582
BfrBI ATG'CAT
Cuts at: 601 601
Size: 6485
BglII A'GATC_T
Cuts at: 1638 4000 6037 1638
Size: 2362 2037 2086
Fragments arranged by size:
2362 2086 2037
BlpI GC'TnA_GC
Cuts at: 3597 3597
Size: 6485
Bme1580I G_kGCm'C
Cuts at: 559 2286 4110 4203 5259 559
Size: 1727 1824 93 1056 1785
Fragments arranged by size:
1824 1785 1727 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2161 2529 3697 3778 4113 6257 2161
Size: 368 1168 81 335 2144 2389
Fragments arranged by size:
2389 2144 1168 368 335 81
BmtI G_CTAG'C
Cuts at: 2596 2596
Size: 6485
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4852 4852
Size: 6485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1859 2993 1859
Size: 1134 5351
Bpu10I CC'TnA_GC
Cuts at: 401 2276 3420 401
Size: 1875 1144 3466
Fragments arranged by size:
3466 1875 1144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1564 3661 4540 5032 5330 1009
Size: 555 2097 879 492 298 2164
Fragments arranged by size:
2164 2097 879 555 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6189 6189
Size: 6485
BsaAI yAC'GTr
Cuts at: 3138 3838 3138
Size: 700 5785
BsaBI GATnn'nnATC
Cuts at: 6 3999 6036 6
Size: 3993 2037 455
Fragments arranged by size:
3993 2037 455
BsaHI Gr'CG_yC
Cuts at: 3151 3916 4164 4866 6362 3151
Size: 765 248 702 1496 3274
Fragments arranged by size:
3274 1496 765 702 248
BsaWI w'CCGG_w
Cuts at: 3674 4195 4873 5147 5294 5705 3674
Size: 521 678 274 147 411 4454
Fragments arranged by size:
4454 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 309 339 1248 1278 1764 1794 309
Size: 30 909 30 486 30 5000
Fragments arranged by size:
5000 909 486 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 333 1256 1772 3244 3247 3316 3319 3322 3325
Size: 923 516 1472 3 69 3 3 3
Cuts at: 3325 333
Size: 3493
Fragments arranged by size:
3493 1472 923 516 69 3 3 3
3
BseYI C'CCAG_C
Cuts at: 353 3910 4668 5245 5741 353
Size: 3557 758 577 496 1097
Fragments arranged by size:
3557 1097 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 741 1949 2044 741
Size: 1208 95 5182
Fragments arranged by size:
5182 1208 95
BsiEI CG_ry'CG
Cuts at: 104 4027 4073 5281 104
Size: 3923 46 1208 1308
Fragments arranged by size:
3923 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 881 1940 2286 3137 3499 4277 4467 5259 881
Size: 1059 346 851 362 778 190 792 2107
Fragments arranged by size:
2107 1059 851 792 778 362 346 190
BsmI GAATG_Cn'
Cuts at: 345 445 919 1920 345
Size: 100 474 1001 4910
Fragments arranged by size:
4910 1001 474 100
BsmBI CGTCTCn'nnnn_
Cuts at: 1055 3158 1055
Size: 2103 4382
Bsp1286I G_dGCh'C
Cuts at: 559 881 1940 2286 3137 3499 4110 4203 4277
Size: 322 1059 346 851 362 611 93 74
Cuts at: 4277 4467 4529 5259 559
Size: 190 62 730 1785
Fragments arranged by size:
1785 1059 851 730 611 362 346 322
190 93 74 62
BspEI T'CCGG_A
Cuts at: 5705 5705
Size: 6485
BspHI T'CATG_A
Cuts at: 576 684 2901 5661 576
Size: 108 2217 2760 1400
Fragments arranged by size:
2760 2217 1400 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 296 3535 3749 4051 4432 296
Size: 3239 214 302 381 2349
Fragments arranged by size:
3239 2349 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4777 4831 6315 6480 50
Size: 4727 54 1484 165 55
Fragments arranged by size:
4727 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2555 4397 2555
Size: 1842 4643
BsrFI r'CCGG_y
Cuts at: 4483 4664 4873 4483
Size: 181 209 6095
Fragments arranged by size:
6095 209 181
BsrGI T'GTAC_A
Cuts at: 421 2267 421
Size: 1846 4639
BssSI C'ACGA_G
Cuts at: 4756 5114 4756
Size: 358 6127
BstAPI GCAn_nnn'nTGC
Cuts at: 1906 3862 1906
Size: 1956 4529
BstBI TT'CG_AA
Cuts at: 4846 4846
Size: 6485
BstXI CCAn_nnnn'nTGG
Cuts at: 2550 2698 2550
Size: 148 6337
BstYI r'GATC_y
Cuts at: 1 1638 3510 3977 4000 4335 4581 5593 5679
Size: 1637 1872 467 23 335 246 1012 86
Cuts at: 5679 5691 6037 1
Size: 12 346 449
Fragments arranged by size:
1872 1637 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 527 1268 527
Size: 741 5744
BtgI C'CryG_G
Cuts at: 107 151 107
Size: 44 6441
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3034 4435 4616 4779 3034
Size: 1401 181 163 4740
Fragments arranged by size:
4740 1401 181 163
BtsI GCAGTG_nn'
Cuts at: 330 509 2528 3728 330
Size: 179 2019 1200 3087
Fragments arranged by size:
3087 2019 1200 179
DraI TTT'AAA
Cuts at: 1583 1874 2931 3484 1583
Size: 291 1057 553 4584
Fragments arranged by size:
4584 1057 553 291
DraIII CAC_nnn'GTG
Cuts at: 839 2494 839
Size: 1655 4830
DrdI GACnn_nn'nnGTC
Cuts at: 514 4191 5049 514
Size: 3677 858 1950
Fragments arranged by size:
3677 1950 858
EaeI y'GGCC_r
Cuts at: 1464 2552 4070 4244 4635 4662 6007 1464
Size: 1088 1518 174 391 27 1345 1942
Fragments arranged by size:
1942 1518 1345 1088 391 174 27
EagI C'GGCC_G
Cuts at: 4070 4070
Size: 6485
EarI CTCTTCn'nnn_
Cuts at: 482 533 777 1928 2285 3098 3194 3221 3293
Size: 51 244 1151 357 813 96 27 72
Cuts at: 3293 3326 3335 4508 4718 482
Size: 33 9 1173 210 2249
Fragments arranged by size:
2249 1173 1151 813 357 244 210 96
72 51 33 27 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 4693 5003 5149 6295 127
Size: 4566 310 146 1146 317
Fragments arranged by size:
4566 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 1938 3497 1938
Size: 1559 4926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 557 1175 1859 2993 3395 3479 4310 4742
Size: 141 618 684 1134 402 84 831 432
Cuts at: 4742 5489 5757 416
Size: 747 268 1144
Fragments arranged by size:
1144 1134 831 747 684 618 432 402
268 141 84
EcoNI CCTnn'n_nnAGG
Cuts at: 2051 2051
Size: 6485
EcoO109I rG'GnC_Cy
Cuts at: 391 3112 3624 391
Size: 2721 512 3252
Fragments arranged by size:
3252 2721 512
EcoRI G'AATT_C
Cuts at: 887 1168 3489 887
Size: 281 2321 3883
Fragments arranged by size:
3883 2321 281
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 951 1144 1429 1899 4504 951
Size: 193 285 470 2605 2932
Fragments arranged by size:
2932 2605 470 285 193
FspI TGC'GCA
Cuts at: 2424 4266 2424
Size: 1842 4643
HaeII r_GCGC'y
Cuts at: 2593 3012 3919 4167 5189 6168 2593
Size: 419 907 248 1022 979 2910
Fragments arranged by size:
2910 1022 979 907 419 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2713 2745 3439 3471 4327 4357 4359 4389 2713
Size: 32 694 32 856 30 2 30 4809
Fragments arranged by size:
4809 856 694 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1137 2357 3524 1137
Size: 1220 1167 4098
Fragments arranged by size:
4098 1220 1167
HindIII A'AGCT_T
Cuts at: 848 1668 848
Size: 820 5665
HpaI GTT'AAC
Cuts at: 1137 1137
Size: 6485
KasI G'GCGC_C
Cuts at: 3915 4163 3915
Size: 248 6237
KpnI G_GTAC'C
Cuts at: 3505 3505
Size: 6485
MluI A'CGCG_T
Cuts at: 5918 5918
Size: 6485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1373 5156 5340 1373
Size: 3783 184 2518
Fragments arranged by size:
3783 2518 184
MscI TGG'CCA
Cuts at: 1466 2554 4246 1466
Size: 1088 1692 3705
Fragments arranged by size:
3705 1692 1088
MslI CAynn'nnrTG
Cuts at: 912 1035 2548 2876 2906 3140 4474 4601 912
Size: 123 1513 328 30 234 1334 127 2796
Fragments arranged by size:
2796 1513 1334 328 234 127 123 30
MspA1I CmG'CkG
Cuts at: 153 259 2275 3596 3910 4270 5283 5528 153
Size: 106 2016 1321 314 360 1013 245 1110
Fragments arranged by size:
2016 1321 1110 1013 360 314 245 106
NaeI GCC'GGC
Cuts at: 4666 4666
Size: 6485
NarI GG'CG_CC
Cuts at: 3916 4164 3916
Size: 248 6237
NcoI C'CATG_G
Cuts at: 107 107
Size: 6485
NdeI CA'TA_TG
Cuts at: 909 909
Size: 6485
NgoMIV G'CCGG_C
Cuts at: 4664 4664
Size: 6485
NheI G'CTAG_C
Cuts at: 2592 2592
Size: 6485
NsiI A_TGCA'T
Cuts at: 603 603
Size: 6485
NspI r_CATG'y
Cuts at: 799 2961 3538 4038 4945 799
Size: 2162 577 500 907 2339
Fragments arranged by size:
2339 2162 907 577 500
PciI A'CATG_T
Cuts at: 795 4941 795
Size: 4146 2339
PflMI CCAn_nnn'nTGG
Cuts at: 1303 1303
Size: 6485
PfoI T'CCnGG_A
Cuts at: 1719 2396 1719
Size: 677 5808
PmeI GTTT'AAAC
Cuts at: 3484 3484
Size: 6485
PmlI CAC'GTG
Cuts at: 3138 3138
Size: 6485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5624 5656 5624
Size: 32 6453
PsiI TTA'TAA
Cuts at: 176 176
Size: 6485
PstI C_TGCA'G
Cuts at: 1797 2664 3532 4217 1797
Size: 867 868 685 4065
Fragments arranged by size:
4065 868 867 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6485
PvuII CAG'CTG
Cuts at: 259 2275 3910 4270 259
Size: 2016 1635 360 2474
Fragments arranged by size:
2474 2016 1635 360
RsrII CG'GwC_CG
Cuts at: 4680 4680
Size: 6485
SacI G_AGCT'C
Cuts at: 1940 3499 1940
Size: 1559 4926
SacII CC_GC'GG
Cuts at: 154 154
Size: 6485
SalI G'TCGA_C
Cuts at: 3522 3522
Size: 6485
SapI GCTCTTCn'nnn_
Cuts at: 1928 2285 4508 4718 1928
Size: 357 2223 210 3695
Fragments arranged by size:
3695 2223 357 210
SbfI CC_TGCA'GG
Cuts at: 3532 3532
Size: 6485
ScaI AGT'ACT
Cuts at: 6002 6002
Size: 6485
SfcI C'TryA_G
Cuts at: 33 954 1536 1793 2522 2660 3528 4213 5206
Size: 921 582 257 729 138 868 685 993
Cuts at: 5206 5397 33
Size: 191 1121
Fragments arranged by size:
1121 993 921 868 729 685 582 257
191 138
SfoI GGC'GCC
Cuts at: 3917 4165 3917
Size: 248 6237
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6485
SmaI CCC'GGG
Cuts at: 3507 3507
Size: 6485
SmlI C'TyrA_G
Cuts at: 882 1024 1543 2583 3640 4555 5047 5309 882
Size: 142 519 1040 1057 915 492 262 2058
Fragments arranged by size:
2058 1057 1040 915 519 492 262 142
SpeI A'CTAG_T
Cuts at: 67 2365 67
Size: 2298 4187
SphI G_CATG'C
Cuts at: 3538 4038 3538
Size: 500 5985
StyI C'CwwG_G
Cuts at: 107 3619 3973 107
Size: 3512 354 2619
Fragments arranged by size:
3512 2619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 800 1464 3130 4068 4201 4660 4869 800
Size: 664 1666 938 133 459 209 2416
Fragments arranged by size:
2416 1666 938 664 459 209 133
TatI w'GTAC_w
Cuts at: 421 1350 2209 2267 6000 421
Size: 929 859 58 3733 906
Fragments arranged by size:
3733 929 906 859 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 309 341 6002 6034 309
Size: 32 5661 32 760
Fragments arranged by size:
5661 760 32 32
Tth111I GACn'n_nGTC
Cuts at: 804 4282 804
Size: 3478 3007
XbaI T'CTAG_A
Cuts at: 3516 3516
Size: 6485
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3980 3980
Size: 6485
XmaI C'CCGG_G
Cuts at: 3505 3505
Size: 6485
XmnI GAAnn'nnTTC
Cuts at: 491 491
Size: 6485
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NsiI NspI PciI PflMI PfoI PmeI PmlI
PpiI PsiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AloI ApaI AscI AvrII BaeI
BglI BmgBI BsiWI BssHII BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI MfeI NotI NruI PacI PasI
PpuMI PshAI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI