Restriction map for
pF1KB4347
(Circular) (Six-base) MAPSORT of: pF1KB4347.seq Check: 2966 from: 1 to: 3749
pF1KB4347 3749 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 787 787
Size: 3749
Acc65I G'GTAC_C
Cuts at: 765 765
Size: 3749
AclI AA'CG_TT
Cuts at: 3589 3589
Size: 3749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 1574 2006 2753 3021 262
Size: 1312 432 747 268 990
Fragments arranged by size:
1312 990 747 432 268
AflIII A'CryG_T
Cuts at: 1101 2205 3182 1101
Size: 1104 977 1668
Fragments arranged by size:
1668 1104 977
AgeI A'CCGG_T
Cuts at: 2137 2137
Size: 3749
AlwNI CAG_nnn'CTG
Cuts at: 1054 2621 1054
Size: 1567 2182
ApaLI G'TGCA_C
Cuts at: 2519 2519
Size: 3749
ApoI r'AATT_y
Cuts at: 605 617 753 3102 3358 605
Size: 12 136 2349 256 996
Fragments arranged by size:
2349 996 256 136 12
AseI AT'TA_AT
Cuts at: 20 590 641 20
Size: 570 51 3128
Fragments arranged by size:
3128 570 51
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
AvaI C'yCGr_G
Cuts at: 769 769
Size: 3749
BamHI G'GATC_C
Cuts at: 774 774
Size: 3749
BanI G'GyrC_C
Cuts at: 765 1179 1427 1462 765
Size: 414 248 35 3052
Fragments arranged by size:
3052 414 248 35
BanII G_rGCy'C
Cuts at: 763 1793 763
Size: 1030 2719
BbeI G_GCGC'C
Cuts at: 1183 1431 1183
Size: 248 3501
BbvCI CC'TCA_GC
Cuts at: 674 674
Size: 3749
BciVI GTATCCnnnnn_n'
Cuts at: 282 1647 2408 3749 282
Size: 1365 761 1341 282
Fragments arranged by size:
1365 1341 761 282
BclI T'GATC_A
Cuts at: 1269 1269
Size: 3749
BglII A'GATC_T
Cuts at: 1264 3301 1264
Size: 2037 1712
BlpI GC'TnA_GC
Cuts at: 861 861
Size: 3749
Bme1580I G_kGCm'C
Cuts at: 1374 1467 2523 1374
Size: 93 1056 2600
Fragments arranged by size:
2600 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 961 1042 1377 3521 961
Size: 81 335 2144 1189
Fragments arranged by size:
2144 1189 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 3749
Bpu10I CC'TnA_GC
Cuts at: 674 674
Size: 3749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1804 2296 2594 925
Size: 879 492 298 2080
Fragments arranged by size:
2080 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3453 3453
Size: 3749
BsaAI yAC'GTr
Cuts at: 1102 1102
Size: 3749
BsaBI GATnn'nnATC
Cuts at: 6 1263 3300 6
Size: 1257 2037 455
Fragments arranged by size:
2037 1257 455
BsaHI Gr'CG_yC
Cuts at: 1180 1428 2130 3626 1180
Size: 248 702 1496 1303
Fragments arranged by size:
1496 1303 702 248
BsaWI w'CCGG_w
Cuts at: 938 1459 2137 2411 2558 2969 938
Size: 521 678 274 147 411 1718
Fragments arranged by size:
1718 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 727 1174 1932 2509 3005 727
Size: 447 758 577 496 1471
Fragments arranged by size:
1471 758 577 496 447
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 362
Size: 3749
BsiEI CG_ry'CG
Cuts at: 104 1291 1337 2545 104
Size: 1187 46 1208 1308
Fragments arranged by size:
1308 1208 1187 46
BsiHKAI G_wGCw'C
Cuts at: 243 763 1541 1731 2523 243
Size: 520 778 190 792 1469
Fragments arranged by size:
1469 792 778 520 190
BsiWI C'GTAC_G
Cuts at: 113 113
Size: 3749
BsmI GAATG_Cn'
Cuts at: 426 426
Size: 3749
Bsp1286I G_dGCh'C
Cuts at: 243 763 1374 1467 1541 1731 1793 2523 243
Size: 520 611 93 74 190 62 730 1469
Fragments arranged by size:
1469 730 611 520 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2969 2969
Size: 3749
BspHI T'CATG_A
Cuts at: 2925 2925
Size: 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 799 1013 1315 1696 799
Size: 214 302 381 2852
Fragments arranged by size:
2852 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2041 2095 3579 3744 50
Size: 1991 54 1484 165 55
Fragments arranged by size:
1991 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1661 1661
Size: 3749
BsrFI r'CCGG_y
Cuts at: 1747 1928 2137 1747
Size: 181 209 3359
Fragments arranged by size:
3359 209 181
BssSI C'ACGA_G
Cuts at: 521 2020 2378 521
Size: 1499 358 1892
Fragments arranged by size:
1892 1499 358
BstAPI GCAn_nnn'nTGC
Cuts at: 700 1126 700
Size: 426 3323
BstBI TT'CG_AA
Cuts at: 2110 2110
Size: 3749
BstYI r'GATC_y
Cuts at: 1 774 1241 1264 1599 1845 2857 2943 2955
Size: 773 467 23 335 246 1012 86 12
Cuts at: 2955 3301 1
Size: 346 449
Fragments arranged by size:
1012 773 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1699 1880 2043 1699
Size: 181 163 3405
Fragments arranged by size:
3405 181 163
BtsI GCAGTG_nn'
Cuts at: 992 992
Size: 3749
DraI TTT'AAA
Cuts at: 748 748
Size: 3749
DrdI GACnn_nn'nnGTC
Cuts at: 1455 2313 1455
Size: 858 2891
EaeI y'GGCC_r
Cuts at: 1334 1508 1899 1926 3271 1334
Size: 174 391 27 1345 1812
Fragments arranged by size:
1812 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1334 1334
Size: 3749
EarI CTCTTCn'nnn_
Cuts at: 130 480 572 1772 1982 130
Size: 350 92 1200 210 1897
Fragments arranged by size:
1897 1200 350 210 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1957 2267 2413 3559 1957
Size: 310 146 1146 2147
Fragments arranged by size:
2147 1146 310 146
EcoICRI GAG'CTC
Cuts at: 761 761
Size: 3749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 1574 2006 2753 3021 262
Size: 1312 432 747 268 990
Fragments arranged by size:
1312 990 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 888 888
Size: 3749
EcoRI G'AATT_C
Cuts at: 753 753
Size: 3749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1768 1768
Size: 3749
FspI TGC'GCA
Cuts at: 1530 1530
Size: 3749
HaeII r_GCGC'y
Cuts at: 1183 1431 2453 3432 1183
Size: 248 1022 979 1500
Fragments arranged by size:
1500 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 463 495 1591 1621 1623 1653 463
Size: 32 1096 30 2 30 2559
Fragments arranged by size:
2559 1096 32 30 30 2
HincII GTy'rAC
Cuts at: 788 788
Size: 3749
HindIII A'AGCT_T
Cuts at: 512 512
Size: 3749
KasI G'GCGC_C
Cuts at: 1179 1427 1179
Size: 248 3501
KpnI G_GTAC'C
Cuts at: 769 769
Size: 3749
MluI A'CGCG_T
Cuts at: 3182 3182
Size: 3749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 461 2420 2604 461
Size: 1959 184 1606
Fragments arranged by size:
1959 1606 184
MscI TGG'CCA
Cuts at: 1510 1510
Size: 3749
MslI CAynn'nnrTG
Cuts at: 541 1738 1865 541
Size: 1197 127 2425
Fragments arranged by size:
2425 1197 127
MspA1I CmG'CkG
Cuts at: 860 1174 1534 2547 2792 860
Size: 314 360 1013 245 1817
Fragments arranged by size:
1817 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1930 1930
Size: 3749
NarI GG'CG_CC
Cuts at: 1180 1428 1180
Size: 248 3501
NcoI C'CATG_G
Cuts at: 107 107
Size: 3749
NgoMIV G'CCGG_C
Cuts at: 1928 1928
Size: 3749
NspI r_CATG'y
Cuts at: 682 703 802 1302 2209 682
Size: 21 99 500 907 2222
Fragments arranged by size:
2222 907 500 99 21
PciI A'CATG_T
Cuts at: 2205 2205
Size: 3749
PmeI GTTT'AAAC
Cuts at: 748 748
Size: 3749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 518 550 2888 2920 518
Size: 32 2338 32 1347
Fragments arranged by size:
2338 1347 32 32
PstI C_TGCA'G
Cuts at: 796 1481 796
Size: 685 3064
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3749
PvuII CAG'CTG
Cuts at: 1174 1534 1174
Size: 360 3389
RsrII CG'GwC_CG
Cuts at: 1944 1944
Size: 3749
SacI G_AGCT'C
Cuts at: 763 763
Size: 3749
SalI G'TCGA_C
Cuts at: 786 786
Size: 3749
SapI GCTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
SbfI CC_TGCA'GG
Cuts at: 796 796
Size: 3749
ScaI AGT'ACT
Cuts at: 3266 3266
Size: 3749
SexAI A'CCwGG_T
Cuts at: 403 403
Size: 3749
SfcI C'TryA_G
Cuts at: 33 178 792 1477 2470 2661 33
Size: 145 614 685 993 191 1121
Fragments arranged by size:
1121 993 685 614 191 145
SfoI GGC'GCC
Cuts at: 1181 1429 1181
Size: 248 3501
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
SmaI CCC'GGG
Cuts at: 771 771
Size: 3749
SmlI C'TyrA_G
Cuts at: 904 1819 2311 2573 904
Size: 915 492 262 2080
Fragments arranged by size:
2080 915 492 262
SpeI A'CTAG_T
Cuts at: 67 357 67
Size: 290 3459
SphI G_CATG'C
Cuts at: 682 802 1302 682
Size: 120 500 3129
Fragments arranged by size:
3129 500 120
StyI C'CwwG_G
Cuts at: 107 883 1237 107
Size: 776 354 2619
Fragments arranged by size:
2619 776 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1332 1465 1924 2133 1332
Size: 133 459 209 2948
Fragments arranged by size:
2948 459 209 133
TatI w'GTAC_w
Cuts at: 131 3264 131
Size: 3133 616
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 3717
Tth111I GACn'n_nGTC
Cuts at: 1546 1546
Size: 3749
XbaI T'CTAG_A
Cuts at: 481 780 481
Size: 299 3450
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 438 1244 438
Size: 806 2943
XmaI C'CCGG_G
Cuts at: 769 769
Size: 3749
XmnI GAAnn'nnTTC
Cuts at: 308 617 308
Size: 309 3440
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BpmI BsaXI BseRI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI