Restriction map for
pF1KB4327
(Circular) (Six-base) MAPSORT of: pF1KB4327.seq Check: 2864 from: 1 to: 4727
pF1KB4327 4727 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 393 393
Size: 4727
AccI GT'mk_AC
Cuts at: 623 1765 623
Size: 1142 3585
Acc65I G'GTAC_C
Cuts at: 1743 1743
Size: 4727
AclI AA'CG_TT
Cuts at: 4567 4567
Size: 4727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2552 2984 3731 3999 2552
Size: 432 747 268 3280
Fragments arranged by size:
3280 747 432 268
AfeI AGC'GCT
Cuts at: 228 228
Size: 4727
AflIII A'CryG_T
Cuts at: 2079 3183 4160 2079
Size: 1104 977 2646
Fragments arranged by size:
2646 1104 977
AgeI A'CCGG_T
Cuts at: 3115 3115
Size: 4727
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1597 1624 1597
Size: 27 4700
AlwNI CAG_nnn'CTG
Cuts at: 915 948 2032 3599 915
Size: 33 1084 1567 2043
Fragments arranged by size:
2043 1567 1084 33
ApaI G_GGCC'C
Cuts at: 526 526
Size: 4727
ApaLI G'TGCA_C
Cuts at: 3497 3497
Size: 4727
ApoI r'AATT_y
Cuts at: 1731 4080 4336 1731
Size: 2349 256 2122
Fragments arranged by size:
2349 2122 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4727
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
AvaI C'yCGr_G
Cuts at: 1612 1747 1612
Size: 135 4592
BamHI G'GATC_C
Cuts at: 878 1752 878
Size: 874 3853
BanI G'GyrC_C
Cuts at: 135 1144 1743 2157 2405 2440 135
Size: 1009 599 414 248 35 2422
Fragments arranged by size:
2422 1009 599 414 248 35
BanII G_rGCy'C
Cuts at: 526 1108 1302 1367 1741 2771 526
Size: 582 194 65 374 1030 2482
Fragments arranged by size:
2482 1030 582 374 194 65
BbeI G_GCGC'C
Cuts at: 139 2161 2409 139
Size: 2022 248 2457
Fragments arranged by size:
2457 2022 248
BbsI GAAGACnn'nnnn_
Cuts at: 1158 1158
Size: 4727
BbvCI CC'TCA_GC
Cuts at: 874 1393 1412 874
Size: 519 19 4189
Fragments arranged by size:
4189 519 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 273 307 273
Size: 34 4693
BciVI GTATCCnnnnn_n'
Cuts at: 2625 3386 4727 2625
Size: 761 1341 2625
Fragments arranged by size:
2625 1341 761
BclI T'GATC_A
Cuts at: 764 1454 2247 764
Size: 690 793 3244
Fragments arranged by size:
3244 793 690
BglI GCCn_nnn'nGGC
Cuts at: 906 934 1202 906
Size: 28 268 4431
Fragments arranged by size:
4431 268 28
BglII A'GATC_T
Cuts at: 1323 2242 4279 1323
Size: 919 2037 1771
Fragments arranged by size:
2037 1771 919
BlpI GC'TnA_GC
Cuts at: 887 1839 887
Size: 952 3775
Bme1580I G_kGCm'C
Cuts at: 526 707 2352 2445 3501 526
Size: 181 1645 93 1056 1752
Fragments arranged by size:
1752 1645 1056 181 93
BmrI ACTGGGnnnn_n'
Cuts at: 1939 2020 2355 4499 1939
Size: 81 335 2144 2167
Fragments arranged by size:
2167 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3094 3094
Size: 4727
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 658 838 970 433
Size: 225 180 132 4190
Fragments arranged by size:
4190 225 180 132
Bpu10I CC'TnA_GC
Cuts at: 874 1393 1412 874
Size: 519 19 4189
Fragments arranged by size:
4189 519 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 1903 2782 3274 3572 304
Size: 1599 879 492 298 1459
Fragments arranged by size:
1599 1459 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1585 4431 1585
Size: 2846 1881
BsaAI yAC'GTr
Cuts at: 1536 2080 1536
Size: 544 4183
BsaBI GATnn'nnATC
Cuts at: 6 2241 4278 6
Size: 2235 2037 455
Fragments arranged by size:
2235 2037 455
BsaHI Gr'CG_yC
Cuts at: 136 212 566 2158 2406 3108 4604 136
Size: 76 354 1592 248 702 1496 259
Fragments arranged by size:
1592 1496 702 354 259 248 76
BsaWI w'CCGG_w
Cuts at: 1916 2437 3115 3389 3536 3947 1916
Size: 521 678 274 147 411 2696
Fragments arranged by size:
2696 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 850 880 850
Size: 30 4697
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 546 871 937 991 1109 1112 1362 1549 546
Size: 325 66 54 118 3 250 187 3724
Fragments arranged by size:
3724 325 250 187 118 66 54 3
BseYI C'CCAG_C
Cuts at: 185 915 1254 1496 2152 2910 3487 3983 185
Size: 730 339 242 656 758 577 496 929
Fragments arranged by size:
929 758 730 656 577 496 339 242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 303
Size: 4727
BsiEI CG_ry'CG
Cuts at: 104 2269 2315 3523 104
Size: 2165 46 1208 1308
Fragments arranged by size:
2165 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 353 1129 1622 1741 2519 2709 3501 353
Size: 776 493 119 778 190 792 1579
Fragments arranged by size:
1579 792 778 776 493 190 119
BsmI GAATG_Cn'
Cuts at: 1565 1565
Size: 4727
Bsp1286I G_dGCh'C
Cuts at: 353 526 707 1108 1129 1302 1367 1622 1741
Size: 173 181 401 21 173 65 255 119
Cuts at: 1741 2352 2445 2519 2709 2771 3501 353
Size: 611 93 74 190 62 730 1579
Fragments arranged by size:
1579 730 611 401 255 190 181 173
173 119 93 74 65 62 21
BspEI T'CCGG_A
Cuts at: 3947 3947
Size: 4727
BspHI T'CATG_A
Cuts at: 3903 3903
Size: 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 393 967 1777 1991 2293 2674 393
Size: 574 810 214 302 381 2446
Fragments arranged by size:
2446 810 574 381 302 214
BsrBI CCG'CTC
Cuts at: 50 772 1487 3019 3073 4557 4722 50
Size: 722 715 1532 54 1484 165 55
Fragments arranged by size:
1532 1484 722 715 165 55 54
BsrDI GCAATG_nn'
Cuts at: 147 312 1055 1235 2639 147
Size: 165 743 180 1404 2235
Fragments arranged by size:
2235 1404 743 180 165
BsrFI r'CCGG_y
Cuts at: 604 896 1231 1643 2725 2906 3115 604
Size: 292 335 412 1082 181 209 2216
Fragments arranged by size:
2216 1082 412 335 292 209 181
BssSI C'ACGA_G
Cuts at: 279 2998 3356 279
Size: 2719 358 1650
Fragments arranged by size:
2719 1650 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1539 2104 1539
Size: 565 4162
BstBI TT'CG_AA
Cuts at: 3088 3088
Size: 4727
BstEII G'GTnAC_C
Cuts at: 463 1176 463
Size: 713 4014
BstYI r'GATC_y
Cuts at: 1 378 419 878 1323 1752 2219 2242 2577
Size: 377 41 459 445 429 467 23 335
Cuts at: 2577 2823 3835 3921 3933 4279 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 459 449 445 429 377 346
335 246 86 41 23 12
BtgI C'CryG_G
Cuts at: 107 903 1181 1440 1515 107
Size: 796 278 259 75 3319
Fragments arranged by size:
3319 796 278 259 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2677 2858 3021 2677
Size: 181 163 4383
Fragments arranged by size:
4383 181 163
BtsI GCAGTG_nn'
Cuts at: 1074 1970 1074
Size: 896 3831
DraI TTT'AAA
Cuts at: 1726 1726
Size: 4727
DraIII CAC_nnn'GTG
Cuts at: 1295 1539 1295
Size: 244 4483
DrdI GACnn_nn'nnGTC
Cuts at: 619 2433 3291 619
Size: 1814 858 2055
Fragments arranged by size:
2055 1814 858
EaeI y'GGCC_r
Cuts at: 369 483 1136 1194 2312 2486 2877 2904 4249
Size: 114 653 58 1118 174 391 27 1345
Cuts at: 4249 369
Size: 847
Fragments arranged by size:
1345 1118 847 653 391 174 114 58
27
EagI C'GGCC_G
Cuts at: 2312 2312
Size: 4727
EarI CTCTTCn'nnn_
Cuts at: 430 790 1372 2750 2960 430
Size: 360 582 1378 210 2197
Fragments arranged by size:
2197 1378 582 360 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2935 3245 3391 4537 2935
Size: 310 146 1146 3125
Fragments arranged by size:
3125 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1739 1739
Size: 4727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 658 838 970 2552 2984 3731 3999 433
Size: 225 180 132 1582 432 747 268 1161
Fragments arranged by size:
1582 1161 747 432 268 225 180 132
EcoNI CCTnn'n_nnAGG
Cuts at: 1091 1612 1091
Size: 521 4206
EcoO109I rG'GnC_Cy
Cuts at: 161 523 936 945 1866 161
Size: 362 413 9 921 3022
Fragments arranged by size:
3022 921 413 362 9
EcoRI G'AATT_C
Cuts at: 1731 1731
Size: 4727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 4727
FspI TGC'GCA
Cuts at: 2508 2508
Size: 4727
HaeII r_GCGC'y
Cuts at: 139 230 248 1474 1503 2161 2409 3431 4410
Size: 91 18 1226 29 658 248 1022 979
Cuts at: 4410 139
Size: 456
Fragments arranged by size:
1226 1022 979 658 456 248 91 29
18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 756 788 850 882 1446 1478 2569 2599 2601
Size: 32 62 32 564 32 1091 30 2
Cuts at: 2601 2631 756
Size: 30 2852
Fragments arranged by size:
2852 1091 564 62 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 612 1766 612
Size: 1154 3573
KasI G'GCGC_C
Cuts at: 135 2157 2405 135
Size: 2022 248 2457
Fragments arranged by size:
2457 2022 248
KpnI G_GTAC'C
Cuts at: 1747 1747
Size: 4727
MluI A'CGCG_T
Cuts at: 4160 4160
Size: 4727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3398 3582 3398
Size: 184 4543
MscI TGG'CCA
Cuts at: 1138 2488 1138
Size: 1350 3377
MslI CAynn'nnrTG
Cuts at: 2716 2843 2716
Size: 127 4600
MspA1I CmG'CkG
Cuts at: 144 209 287 1142 1679 1695 1838 2152 2512
Size: 65 78 855 537 16 143 314 360
Cuts at: 2512 3525 3770 144
Size: 1013 245 1101
Fragments arranged by size:
1101 1013 855 537 360 314 245 143
78 65 16
NaeI GCC'GGC
Cuts at: 1645 2908 1645
Size: 1263 3464
NarI GG'CG_CC
Cuts at: 136 2158 2406 136
Size: 2022 248 2457
Fragments arranged by size:
2457 2022 248
NcoI C'CATG_G
Cuts at: 107 903 1515 107
Size: 796 612 3319
Fragments arranged by size:
3319 796 612
NgoMIV G'CCGG_C
Cuts at: 1643 2906 1643
Size: 1263 3464
NspI r_CATG'y
Cuts at: 1780 2280 3187 1780
Size: 500 907 3320
Fragments arranged by size:
3320 907 500
PciI A'CATG_T
Cuts at: 3183 3183
Size: 4727
PflMI CCAn_nnn'nTGG
Cuts at: 1089 1295 1089
Size: 206 4521
PfoI T'CCnGG_A
Cuts at: 410 815 1622 410
Size: 405 807 3515
Fragments arranged by size:
3515 807 405
PmeI GTTT'AAAC
Cuts at: 1726 1726
Size: 4727
PmlI CAC'GTG
Cuts at: 1536 1536
Size: 4727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4695
PpuMI rG'GwC_Cy
Cuts at: 945 945
Size: 4727
PspOMI G'GGCC_C
Cuts at: 522 522
Size: 4727
PstI C_TGCA'G
Cuts at: 552 944 1532 1774 2459 552
Size: 392 588 242 685 2820
Fragments arranged by size:
2820 685 588 392 242
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4727
PvuII CAG'CTG
Cuts at: 2152 2512 2152
Size: 360 4367
RsrII CG'GwC_CG
Cuts at: 2922 2922
Size: 4727
SacI G_AGCT'C
Cuts at: 1741 1741
Size: 4727
SalI G'TCGA_C
Cuts at: 1764 1764
Size: 4727
SapI GCTCTTCn'nnn_
Cuts at: 1372 2750 2960 1372
Size: 1378 210 3139
Fragments arranged by size:
3139 1378 210
SbfI CC_TGCA'GG
Cuts at: 944 1774 944
Size: 830 3897
ScaI AGT'ACT
Cuts at: 4244 4244
Size: 4727
SfcI C'TryA_G
Cuts at: 33 548 754 940 1528 1770 2455 3448 3639
Size: 515 206 186 588 242 685 993 191
Cuts at: 3639 33
Size: 1121
Fragments arranged by size:
1121 993 685 588 515 242 206 191
186
SfiI GGCCn_nnn'nGGCC
Cuts at: 934 934
Size: 4727
SfoI GGC'GCC
Cuts at: 137 2159 2407 137
Size: 2022 248 2457
Fragments arranged by size:
2457 2022 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
SmaI CCC'GGG
Cuts at: 1749 1749
Size: 4727
SmlI C'TyrA_G
Cuts at: 319 1882 2797 3289 3551 319
Size: 1563 915 492 262 1495
Fragments arranged by size:
1563 1495 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4727
SphI G_CATG'C
Cuts at: 1780 2280 1780
Size: 500 4227
StyI C'CwwG_G
Cuts at: 107 509 903 1359 1515 1861 2215 107
Size: 402 394 456 156 346 354 2619
Fragments arranged by size:
2619 456 402 394 354 346 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 607 1202 2310 2443 2902 3111 607
Size: 595 1108 133 459 209 2223
Fragments arranged by size:
2223 1108 595 459 209 133
TatI w'GTAC_w
Cuts at: 4242 4242
Size: 4727
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 370 402 4244 4276 370
Size: 32 3842 32 821
Fragments arranged by size:
3842 821 32 32
Tth111I GACn'n_nGTC
Cuts at: 2524 2524
Size: 4727
XbaI T'CTAG_A
Cuts at: 1758 1758
Size: 4727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 129 567 1513 2222 129
Size: 438 946 709 2634
Fragments arranged by size:
2634 946 709 438
XmaI C'CCGG_G
Cuts at: 1747 1747
Size: 4727
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI