Restriction map for
pF1KB4308
(Circular) (Six-base) MAPSORT of: pF1KB4308.seq Check: 9463 from: 1 to: 3662
pF1KB4308 3662 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 700 700
Size: 3662
Acc65I G'GTAC_C
Cuts at: 678 678
Size: 3662
AclI AA'CG_TT
Cuts at: 3502 3502
Size: 3662
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 1487 1919 2666 2934 595
Size: 892 432 747 268 1323
Fragments arranged by size:
1323 892 747 432 268
AflIII A'CryG_T
Cuts at: 1014 2118 3095 1014
Size: 1104 977 1581
Fragments arranged by size:
1581 1104 977
AgeI A'CCGG_T
Cuts at: 2050 2050
Size: 3662
AleI CACnn'nnGTG
Cuts at: 395 395
Size: 3662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 533 533
Size: 3662
AlwNI CAG_nnn'CTG
Cuts at: 183 354 425 967 2534 183
Size: 171 71 542 1567 1311
Fragments arranged by size:
1567 1311 542 171 71
ApaI G_GGCC'C
Cuts at: 140 140
Size: 3662
ApaLI G'TGCA_C
Cuts at: 2432 2432
Size: 3662
ApoI r'AATT_y
Cuts at: 666 3015 3271 666
Size: 2349 256 1057
Fragments arranged by size:
2349 1057 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3662
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
AvaI C'yCGr_G
Cuts at: 682 682
Size: 3662
BamHI G'GATC_C
Cuts at: 687 687
Size: 3662
BanI G'GyrC_C
Cuts at: 397 678 1092 1340 1375 397
Size: 281 414 248 35 2684
Fragments arranged by size:
2684 414 281 248 35
BanII G_rGCy'C
Cuts at: 140 676 1706 140
Size: 536 1030 2096
Fragments arranged by size:
2096 1030 536
BbeI G_GCGC'C
Cuts at: 1096 1344 1096
Size: 248 3414
BciVI GTATCCnnnnn_n'
Cuts at: 1560 2321 3662 1560
Size: 761 1341 1560
Fragments arranged by size:
1560 1341 761
BclI T'GATC_A
Cuts at: 1182 1182
Size: 3662
BglI GCCn_nnn'nGGC
Cuts at: 577 577
Size: 3662
BglII A'GATC_T
Cuts at: 1177 3214 1177
Size: 2037 1625
BlpI GC'TnA_GC
Cuts at: 774 774
Size: 3662
Bme1580I G_kGCm'C
Cuts at: 140 335 434 1287 1380 2436 140
Size: 195 99 853 93 1056 1366
Fragments arranged by size:
1366 1056 853 195 99 93
BmrI ACTGGGnnnn_n'
Cuts at: 874 955 1290 3434 874
Size: 81 335 2144 1102
Fragments arranged by size:
2144 1102 335 81
BmtI G_CTAG'C
Cuts at: 607 607
Size: 3662
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 3662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 205
Size: 3662
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 1717 2209 2507 838
Size: 879 492 298 1993
Fragments arranged by size:
1993 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 227 3366 227
Size: 3139 523
BsaAI yAC'GTr
Cuts at: 1015 1015
Size: 3662
BsaBI GATnn'nnATC
Cuts at: 6 1176 3213 6
Size: 1170 2037 455
Fragments arranged by size:
2037 1170 455
BsaHI Gr'CG_yC
Cuts at: 115 1093 1341 2043 3539 115
Size: 978 248 702 1496 238
Fragments arranged by size:
1496 978 702 248 238
BsaWI w'CCGG_w
Cuts at: 851 1372 2050 2324 2471 2882 851
Size: 521 678 274 147 411 1631
Fragments arranged by size:
1631 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 529 559 529
Size: 30 3632
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 541 202
Size: 339 3323
BseYI C'CCAG_C
Cuts at: 154 567 1087 1845 2422 2918 154
Size: 413 520 758 577 496 898
Fragments arranged by size:
898 758 577 520 496 413
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 394 295
Size: 99 3563
BsiEI CG_ry'CG
Cuts at: 104 296 1204 1250 2458 104
Size: 192 908 46 1208 1308
Fragments arranged by size:
1308 1208 908 192 46
BsiHKAI G_wGCw'C
Cuts at: 167 676 1454 1644 2436 167
Size: 509 778 190 792 1393
Fragments arranged by size:
1393 792 778 509 190
Bsp1286I G_dGCh'C
Cuts at: 140 167 335 434 676 1287 1380 1454 1644
Size: 27 168 99 242 611 93 74 190
Cuts at: 1644 1706 2436 140
Size: 62 730 1366
Fragments arranged by size:
1366 730 611 242 190 168 99 93
74 62 27
BspEI T'CCGG_A
Cuts at: 2882 2882
Size: 3662
BspHI T'CATG_A
Cuts at: 2838 2838
Size: 3662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 623 644 653 712 926 1228 1609 623
Size: 21 9 59 214 302 381 2676
Fragments arranged by size:
2676 381 302 214 59 21 9
BsrBI CCG'CTC
Cuts at: 50 1954 2008 3492 3657 50
Size: 1904 54 1484 165 55
Fragments arranged by size:
1904 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 333 1574 333
Size: 1241 2421
BsrFI r'CCGG_y
Cuts at: 295 1660 1841 2050 295
Size: 1365 181 209 1907
Fragments arranged by size:
1907 1365 209 181
BssSI C'ACGA_G
Cuts at: 1933 2291 1933
Size: 358 3304
BstAPI GCAn_nnn'nTGC
Cuts at: 1039 1039
Size: 3662
BstBI TT'CG_AA
Cuts at: 2023 2023
Size: 3662
BstXI CCAn_nnnn'nTGG
Cuts at: 483 483
Size: 3662
BstYI r'GATC_y
Cuts at: 1 687 1154 1177 1512 1758 2770 2856 2868
Size: 686 467 23 335 246 1012 86 12
Cuts at: 2868 3214 1
Size: 346 449
Fragments arranged by size:
1012 686 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 390 107
Size: 283 3379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1612 1793 1956 1612
Size: 181 163 3318
Fragments arranged by size:
3318 181 163
BtsI GCAGTG_nn'
Cuts at: 302 401 905 302
Size: 99 504 3059
Fragments arranged by size:
3059 504 99
DraI TTT'AAA
Cuts at: 661 661
Size: 3662
DrdI GACnn_nn'nnGTC
Cuts at: 1368 2226 1368
Size: 858 2804
EaeI y'GGCC_r
Cuts at: 293 1247 1421 1812 1839 3184 293
Size: 954 174 391 27 1345 771
Fragments arranged by size:
1345 954 771 391 174 27
EagI C'GGCC_G
Cuts at: 293 1247 293
Size: 954 2708
EarI CTCTTCn'nnn_
Cuts at: 1685 1895 1685
Size: 210 3452
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1870 2180 2326 3472 1870
Size: 310 146 1146 2060
Fragments arranged by size:
2060 1146 310 146
EcoICRI GAG'CTC
Cuts at: 674 674
Size: 3662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 595 1487 1919 2666 2934 205
Size: 390 892 432 747 268 933
Fragments arranged by size:
933 892 747 432 390 268
EcoO109I rG'GnC_Cy
Cuts at: 137 273 642 801 137
Size: 136 369 159 2998
Fragments arranged by size:
2998 369 159 136
EcoRI G'AATT_C
Cuts at: 666 666
Size: 3662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1681 1681
Size: 3662
FspI TGC'GCA
Cuts at: 1443 1443
Size: 3662
HaeII r_GCGC'y
Cuts at: 1096 1344 2366 3345 1096
Size: 248 1022 979 1413
Fragments arranged by size:
1413 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 529 561 634 666 1504 1534 1536 1566 529
Size: 32 73 32 838 30 2 30 2625
Fragments arranged by size:
2625 838 73 32 32 30 30 2
HincII GTy'rAC
Cuts at: 701 701
Size: 3662
KasI G'GCGC_C
Cuts at: 1092 1340 1092
Size: 248 3414
KpnI G_GTAC'C
Cuts at: 682 682
Size: 3662
MluI A'CGCG_T
Cuts at: 3095 3095
Size: 3662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 494 2333 2517 494
Size: 1839 184 1639
Fragments arranged by size:
1839 1639 184
MscI TGG'CCA
Cuts at: 1423 1423
Size: 3662
MslI CAynn'nnrTG
Cuts at: 395 1651 1778 395
Size: 1256 127 2279
Fragments arranged by size:
2279 1256 127
MspA1I CmG'CkG
Cuts at: 773 1087 1447 2460 2705 773
Size: 314 360 1013 245 1730
Fragments arranged by size:
1730 1013 360 314 245
NaeI GCC'GGC
Cuts at: 297 1843 297
Size: 1546 2116
NarI GG'CG_CC
Cuts at: 1093 1341 1093
Size: 248 3414
NcoI C'CATG_G
Cuts at: 107 107
Size: 3662
NgoMIV G'CCGG_C
Cuts at: 295 1841 295
Size: 1546 2116
NheI G'CTAG_C
Cuts at: 603 603
Size: 3662
NspI r_CATG'y
Cuts at: 611 715 1215 2122 611
Size: 104 500 907 2151
Fragments arranged by size:
2151 907 500 104
PasI CC'CwG_GG
Cuts at: 476 476
Size: 3662
PciI A'CATG_T
Cuts at: 2118 2118
Size: 3662
PfoI T'CCnGG_A
Cuts at: 584 584
Size: 3662
PmeI GTTT'AAAC
Cuts at: 661 661
Size: 3662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2801 2833 2801
Size: 32 3630
PpuMI rG'GwC_Cy
Cuts at: 273 642 273
Size: 369 3293
PshAI GACnn'nnGTC
Cuts at: 278 278
Size: 3662
PspOMI G'GGCC_C
Cuts at: 136 136
Size: 3662
PstI C_TGCA'G
Cuts at: 709 1394 709
Size: 685 2977
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3662
PvuII CAG'CTG
Cuts at: 1087 1447 1087
Size: 360 3302
RsrII CG'GwC_CG
Cuts at: 1857 1857
Size: 3662
SacI G_AGCT'C
Cuts at: 676 676
Size: 3662
SalI G'TCGA_C
Cuts at: 699 699
Size: 3662
SapI GCTCTTCn'nnn_
Cuts at: 1685 1895 1685
Size: 210 3452
SbfI CC_TGCA'GG
Cuts at: 709 709
Size: 3662
ScaI AGT'ACT
Cuts at: 3179 3179
Size: 3662
SfcI C'TryA_G
Cuts at: 33 705 1390 2383 2574 33
Size: 672 685 993 191 1121
Fragments arranged by size:
1121 993 685 672 191
SfoI GGC'GCC
Cuts at: 1094 1342 1094
Size: 248 3414
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
SmaI CCC'GGG
Cuts at: 684 684
Size: 3662
SmlI C'TyrA_G
Cuts at: 817 1732 2224 2486 817
Size: 915 492 262 1993
Fragments arranged by size:
1993 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3662
SphI G_CATG'C
Cuts at: 715 1215 715
Size: 500 3162
StyI C'CwwG_G
Cuts at: 107 796 1150 107
Size: 689 354 2619
Fragments arranged by size:
2619 689 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 605 1245 1378 1837 2046 605
Size: 640 133 459 209 2221
Fragments arranged by size:
2221 640 459 209 133
TatI w'GTAC_w
Cuts at: 3177 3177
Size: 3662
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 3630
Tth111I GACn'n_nGTC
Cuts at: 1459 1459
Size: 3662
XbaI T'CTAG_A
Cuts at: 693 693
Size: 3662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 477 1157 477
Size: 680 2982
XmaI C'CCGG_G
Cuts at: 682 682
Size: 3662
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PciI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI Bpu10I BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PflMI PmlI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI