Restriction map for
pF1KB4286
(Circular) (Six-base) MAPSORT of: pF1KB4286.seq Check: 4152 from: 1 to: 3818
pF1KB4286 3818 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 856 856
Size: 3818
Acc65I G'GTAC_C
Cuts at: 834 834
Size: 3818
AclI AA'CG_TT
Cuts at: 3658 3658
Size: 3818
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 387 588 1643 2075 2822 3090 247
Size: 140 201 1055 432 747 268 975
Fragments arranged by size:
1055 975 747 432 268 201 140
AflIII A'CryG_T
Cuts at: 1170 2274 3251 1170
Size: 1104 977 1737
Fragments arranged by size:
1737 1104 977
AgeI A'CCGG_T
Cuts at: 2206 2206
Size: 3818
AlwNI CAG_nnn'CTG
Cuts at: 1123 2690 1123
Size: 1567 2251
ApaLI G'TGCA_C
Cuts at: 2588 2588
Size: 3818
ApoI r'AATT_y
Cuts at: 558 822 3171 3427 558
Size: 264 2349 256 949
Fragments arranged by size:
2349 949 264 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3818
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3818
AvaI C'yCGr_G
Cuts at: 838 838
Size: 3818
BamHI G'GATC_C
Cuts at: 794 843 794
Size: 49 3769
BanI G'GyrC_C
Cuts at: 834 1248 1496 1531 834
Size: 414 248 35 3121
Fragments arranged by size:
3121 414 248 35
BanII G_rGCy'C
Cuts at: 832 1862 832
Size: 1030 2788
BbeI G_GCGC'C
Cuts at: 1252 1500 1252
Size: 248 3570
BciVI GTATCCnnnnn_n'
Cuts at: 1716 2477 3818 1716
Size: 761 1341 1716
Fragments arranged by size:
1716 1341 761
BclI T'GATC_A
Cuts at: 1338 1338
Size: 3818
BglII A'GATC_T
Cuts at: 1333 3370 1333
Size: 2037 1781
BlpI GC'TnA_GC
Cuts at: 930 930
Size: 3818
Bme1580I G_kGCm'C
Cuts at: 1443 1536 2592 1443
Size: 93 1056 2669
Fragments arranged by size:
2669 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1030 1111 1446 3590 1030
Size: 81 335 2144 1258
Fragments arranged by size:
2144 1258 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 268 2185 268
Size: 1917 1901
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 433
Size: 3818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 1873 2365 2663 994
Size: 879 492 298 2149
Fragments arranged by size:
2149 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3522 3522
Size: 3818
BsaAI yAC'GTr
Cuts at: 1171 1171
Size: 3818
BsaBI GATnn'nnATC
Cuts at: 6 1332 3369 6
Size: 1326 2037 455
Fragments arranged by size:
2037 1326 455
BsaHI Gr'CG_yC
Cuts at: 1249 1497 2199 3695 1249
Size: 248 702 1496 1372
Fragments arranged by size:
1496 1372 702 248
BsaWI w'CCGG_w
Cuts at: 1007 1528 2206 2480 2627 3038 1007
Size: 521 678 274 147 411 1787
Fragments arranged by size:
1787 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 478 481 478
Size: 3 3815
BseYI C'CCAG_C
Cuts at: 300 730 1243 2001 2578 3074 300
Size: 430 513 758 577 496 1044
Fragments arranged by size:
1044 758 577 513 496 430
BsiEI CG_ry'CG
Cuts at: 104 1360 1406 2614 104
Size: 1256 46 1208 1308
Fragments arranged by size:
1308 1256 1208 46
BsiHKAI G_wGCw'C
Cuts at: 280 535 832 1610 1800 2592 280
Size: 255 297 778 190 792 1506
Fragments arranged by size:
1506 792 778 297 255 190
BsmI GAATG_Cn'
Cuts at: 375 375
Size: 3818
Bsp1286I G_dGCh'C
Cuts at: 280 535 832 1443 1536 1610 1800 1862 2592
Size: 255 297 611 93 74 190 62 730
Cuts at: 2592 280
Size: 1506
Fragments arranged by size:
1506 730 611 297 255 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3038 3038
Size: 3818
BspHI T'CATG_A
Cuts at: 284 2994 284
Size: 2710 1108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 868 1082 1384 1765 868
Size: 214 302 381 2921
Fragments arranged by size:
2921 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2110 2164 3648 3813 50
Size: 2060 54 1484 165 55
Fragments arranged by size:
2060 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1730 1730
Size: 3818
BsrFI r'CCGG_y
Cuts at: 1816 1997 2206 1816
Size: 181 209 3428
Fragments arranged by size:
3428 209 181
BssSI C'ACGA_G
Cuts at: 2089 2447 2089
Size: 358 3460
BstAPI GCAn_nnn'nTGC
Cuts at: 748 1195 748
Size: 447 3371
BstBI TT'CG_AA
Cuts at: 2179 2179
Size: 3818
BstXI CCAn_nnnn'nTGG
Cuts at: 422 579 422
Size: 157 3661
BstYI r'GATC_y
Cuts at: 1 794 843 1310 1333 1668 1914 2926 3012
Size: 793 49 467 23 335 246 1012 86
Cuts at: 3012 3024 3370 1
Size: 12 346 449
Fragments arranged by size:
1012 793 467 449 346 335 246 86
49 23 12
BtgI C'CryG_G
Cuts at: 752 752
Size: 3818
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1768 1949 2112 1768
Size: 181 163 3474
Fragments arranged by size:
3474 181 163
BtsI GCAGTG_nn'
Cuts at: 1061 1061
Size: 3818
DraI TTT'AAA
Cuts at: 817 817
Size: 3818
DrdI GACnn_nn'nnGTC
Cuts at: 348 1524 2382 348
Size: 1176 858 1784
Fragments arranged by size:
1784 1176 858
EaeI y'GGCC_r
Cuts at: 126 712 1403 1577 1968 1995 3340 126
Size: 586 691 174 391 27 1345 604
Fragments arranged by size:
1345 691 604 586 391 174 27
EagI C'GGCC_G
Cuts at: 1403 1403
Size: 3818
EarI CTCTTCn'nnn_
Cuts at: 141 341 500 649 712 1841 2051 141
Size: 200 159 149 63 1129 210 1908
Fragments arranged by size:
1908 1129 210 200 159 149 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2026 2336 2482 3628 2026
Size: 310 146 1146 2216
Fragments arranged by size:
2216 1146 310 146
EcoICRI GAG'CTC
Cuts at: 830 830
Size: 3818
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 387 433 588 1643 2075 2822 3090 247
Size: 140 46 155 1055 432 747 268 975
Fragments arranged by size:
1055 975 747 432 268 155 140 46
EcoO109I rG'GnC_Cy
Cuts at: 957 957
Size: 3818
EcoRI G'AATT_C
Cuts at: 822 822
Size: 3818
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1837 1837
Size: 3818
FspI TGC'GCA
Cuts at: 1599 1599
Size: 3818
HaeII r_GCGC'y
Cuts at: 1252 1500 2522 3501 1252
Size: 248 1022 979 1569
Fragments arranged by size:
1569 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1660 1690 1692 1722 1660
Size: 30 2 30 3756
Fragments arranged by size:
3756 30 30 2
HincII GTy'rAC
Cuts at: 462 857 462
Size: 395 3423
HpaI GTT'AAC
Cuts at: 462 462
Size: 3818
KasI G'GCGC_C
Cuts at: 1248 1496 1248
Size: 248 3570
KpnI G_GTAC'C
Cuts at: 838 838
Size: 3818
MfeI C'AATT_G
Cuts at: 743 743
Size: 3818
MluI A'CGCG_T
Cuts at: 3251 3251
Size: 3818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 710 822 2489 2673 710
Size: 112 1667 184 1855
Fragments arranged by size:
1855 1667 184 112
MscI TGG'CCA
Cuts at: 714 1579 714
Size: 865 2953
MslI CAynn'nnrTG
Cuts at: 286 1807 1934 286
Size: 1521 127 2170
Fragments arranged by size:
2170 1521 127
MspA1I CmG'CkG
Cuts at: 407 929 1243 1603 2616 2861 407
Size: 522 314 360 1013 245 1364
Fragments arranged by size:
1364 1013 522 360 314 245
NaeI GCC'GGC
Cuts at: 1999 1999
Size: 3818
NarI GG'CG_CC
Cuts at: 1249 1497 1249
Size: 248 3570
NcoI C'CATG_G
Cuts at: 752 752
Size: 3818
NgoMIV G'CCGG_C
Cuts at: 1997 1997
Size: 3818
NspI r_CATG'y
Cuts at: 330 871 1371 2278 330
Size: 541 500 907 1870
Fragments arranged by size:
1870 907 541 500
PciI A'CATG_T
Cuts at: 2274 2274
Size: 3818
PfoI T'CCnGG_A
Cuts at: 197 197
Size: 3818
PmeI GTTT'AAAC
Cuts at: 817 817
Size: 3818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 2989 2957
Size: 32 3786
PstI C_TGCA'G
Cuts at: 865 1550 865
Size: 685 3133
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3818
PvuII CAG'CTG
Cuts at: 407 1243 1603 407
Size: 836 360 2622
Fragments arranged by size:
2622 836 360
RsrII CG'GwC_CG
Cuts at: 2013 2013
Size: 3818
SacI G_AGCT'C
Cuts at: 832 832
Size: 3818
SalI G'TCGA_C
Cuts at: 855 855
Size: 3818
SapI GCTCTTCn'nnn_
Cuts at: 1841 2051 1841
Size: 210 3608
SbfI CC_TGCA'GG
Cuts at: 865 865
Size: 3818
ScaI AGT'ACT
Cuts at: 3335 3335
Size: 3818
SfcI C'TryA_G
Cuts at: 33 678 861 1546 2539 2730 33
Size: 645 183 685 993 191 1121
Fragments arranged by size:
1121 993 685 645 191 183
SfoI GGC'GCC
Cuts at: 1250 1498 1250
Size: 248 3570
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3818
SmaI CCC'GGG
Cuts at: 840 840
Size: 3818
SmlI C'TyrA_G
Cuts at: 973 1888 2380 2642 973
Size: 915 492 262 2149
Fragments arranged by size:
2149 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3818
SphI G_CATG'C
Cuts at: 871 1371 871
Size: 500 3318
SspI AAT'ATT
Cuts at: 168 168
Size: 3818
StyI C'CwwG_G
Cuts at: 752 952 1306 752
Size: 200 354 3264
Fragments arranged by size:
3264 354 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 257 358 1401 1534 1993 2202 257
Size: 101 1043 133 459 209 1873
Fragments arranged by size:
1873 1043 459 209 133 101
TatI w'GTAC_w
Cuts at: 3333 3333
Size: 3818
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3335 3367 3335
Size: 32 3786
Tth111I GACn'n_nGTC
Cuts at: 1615 1615
Size: 3818
XbaI T'CTAG_A
Cuts at: 849 849
Size: 3818
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1313 1313
Size: 3818
XmaI C'CCGG_G
Cuts at: 838 838
Size: 3818
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI