Restriction map for
pF1KB4274
(Circular) (Six-base) MAPSORT of: pF1KB4274.seq Check: 5445 from: 1 to: 5054
pF1KB4274 5054 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 795 2092 795
Size: 1297 3757
Acc65I G'GTAC_C
Cuts at: 2070 2070
Size: 5054
AclI AA'CG_TT
Cuts at: 4894 4894
Size: 5054
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 665 1128 2879 3311 3664 4326 368
Size: 297 463 1751 432 353 662 1096
Fragments arranged by size:
1751 1096 662 463 432 353 297
AflIII A'CryG_T
Cuts at: 2406 4206 4487 2406
Size: 1800 281 2973
Fragments arranged by size:
2973 1800 281
AleI CACnn'nnGTG
Cuts at: 1943 1943
Size: 5054
AlwNI CAG_nnn'CTG
Cuts at: 174 2359 3797 174
Size: 2185 1438 1431
Fragments arranged by size:
2185 1438 1431
ApaLI G'TGCA_C
Cuts at: 3892 3892
Size: 5054
ApoI r'AATT_y
Cuts at: 364 483 926 1682 2058 4407 4663 364
Size: 119 443 756 376 2349 256 755
Fragments arranged by size:
2349 756 755 443 376 256 119
AseI AT'TA_AT
Cuts at: 20 547 20
Size: 527 4527
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5054
AvaI C'yCGr_G
Cuts at: 2074 2074
Size: 5054
BamHI G'GATC_C
Cuts at: 2079 2079
Size: 5054
BanI G'GyrC_C
Cuts at: 2070 2484 2732 2767 2070
Size: 414 248 35 4357
Fragments arranged by size:
4357 414 248 35
BanII G_rGCy'C
Cuts at: 2068 3098 2068
Size: 1030 4024
BbeI G_GCGC'C
Cuts at: 2488 2736 2488
Size: 248 4806
BbsI GAAGACnn'nnnn_
Cuts at: 196 356 1243 1411 1495 1915 196
Size: 160 887 168 84 420 3335
Fragments arranged by size:
3335 887 420 168 160 84
BciVI GTATCCnnnnn_n'
Cuts at: 2952 4008 5054 2952
Size: 1056 1046 2952
Fragments arranged by size:
2952 1056 1046
BclI T'GATC_A
Cuts at: 999 1083 1587 2574 999
Size: 84 504 987 3479
Fragments arranged by size:
3479 987 504 84
BfrBI ATG'CAT
Cuts at: 973 973
Size: 5054
BglII A'GATC_T
Cuts at: 2569 4606 2569
Size: 2037 3017
BlpI GC'TnA_GC
Cuts at: 2166 2166
Size: 5054
Bme1580I G_kGCm'C
Cuts at: 2679 2772 3896 2679
Size: 93 1124 3837
Fragments arranged by size:
3837 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2266 2347 2682 4826 2266
Size: 81 335 2144 2494
Fragments arranged by size:
2494 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3421 3421
Size: 5054
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 707 707
Size: 5054
Bpu10I CC'TnA_GC
Cuts at: 113 113
Size: 5054
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 1051 2230 3109 3823 4121 259
Size: 792 1179 879 714 298 1192
Fragments arranged by size:
1192 1179 879 792 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4758 4758
Size: 5054
BsaAI yAC'GTr
Cuts at: 2407 2407
Size: 5054
BsaBI GATnn'nnATC
Cuts at: 6 2568 4605 6
Size: 2562 2037 455
Fragments arranged by size:
2562 2037 455
BsaHI Gr'CG_yC
Cuts at: 2485 2733 3435 4931 2485
Size: 248 702 1496 2608
Fragments arranged by size:
2608 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2243 2764 3442 3853 4000 4274 2243
Size: 521 678 411 147 274 3023
Fragments arranged by size:
3023 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 705 735 1099 1129 705
Size: 30 364 30 4630
Fragments arranged by size:
4630 364 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 302 1456 175
Size: 127 1154 3773
Fragments arranged by size:
3773 1154 127
BseYI C'CCAG_C
Cuts at: 2479 3237 3902 4310 2479
Size: 758 665 408 3223
Fragments arranged by size:
3223 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2596 2642 3872 104
Size: 2492 46 1230 1286
Fragments arranged by size:
2492 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2068 2846 3036 3896 2068
Size: 778 190 860 3226
Fragments arranged by size:
3226 860 778 190
BsmI GAATG_Cn'
Cuts at: 1143 1143
Size: 5054
Bsp1286I G_dGCh'C
Cuts at: 2068 2679 2772 2846 3036 3098 3896 2068
Size: 611 93 74 190 62 798 3226
Fragments arranged by size:
3226 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1422 3486 1422
Size: 2064 2990
BspMI ACCTGCnnnn'nnnn_
Cuts at: 304 331 2104 2318 2620 3001 304
Size: 27 1773 214 302 381 2357
Fragments arranged by size:
2357 1773 381 302 214 27
BsrBI CCG'CTC
Cuts at: 50 3346 3400 4884 5049 50
Size: 3296 54 1484 165 55
Fragments arranged by size:
3296 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 879 1047 1135 2966 879
Size: 168 88 1831 2967
Fragments arranged by size:
2967 1831 168 88
BsrFI r'CCGG_y
Cuts at: 1774 3052 3233 1774
Size: 1278 181 3595
Fragments arranged by size:
3595 1278 181
BsrGI T'GTAC_A
Cuts at: 1335 1755 1923 1335
Size: 420 168 4466
Fragments arranged by size:
4466 420 168
BssSI C'ACGA_G
Cuts at: 3325 4033 3325
Size: 708 4346
BstAPI GCAn_nnn'nTGC
Cuts at: 2431 2431
Size: 5054
BstBI TT'CG_AA
Cuts at: 3415 3415
Size: 5054
BstYI r'GATC_y
Cuts at: 1 2079 2546 2569 2904 3150 3456 3468 3554
Size: 2078 467 23 335 246 306 12 86
Cuts at: 3554 4606 1
Size: 1052 449
Fragments arranged by size:
2078 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 298 107
Size: 191 4863
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3004 3185 3348 3004
Size: 181 163 4710
Fragments arranged by size:
4710 181 163
BtsI GCAGTG_nn'
Cuts at: 2297 2297
Size: 5054
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 861 896 1029 1064 861
Size: 35 133 35 4851
Fragments arranged by size:
4851 133 35 35
DraI TTT'AAA
Cuts at: 2053 2053
Size: 5054
DrdI GACnn_nn'nnGTC
Cuts at: 2760 4104 2760
Size: 1344 3710
EaeI y'GGCC_r
Cuts at: 1776 2639 2813 3204 3231 4576 1776
Size: 863 174 391 27 1345 2254
Fragments arranged by size:
2254 1345 863 391 174 27
EagI C'GGCC_G
Cuts at: 2639 2639
Size: 5054
EarI CTCTTCn'nnn_
Cuts at: 287 430 834 1424 3077 3287 287
Size: 143 404 590 1653 210 2054
Fragments arranged by size:
2054 1653 590 404 210 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1051 1219 1471 1891 3262 4004 4150 4864 1051
Size: 168 252 420 1371 742 146 714 1241
Fragments arranged by size:
1371 1241 742 714 420 252 168 146
EcoICRI GAG'CTC
Cuts at: 2066 2066
Size: 5054
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 665 707 1128 2879 3311 3664 4326 368
Size: 297 42 421 1751 432 353 662 1096
Fragments arranged by size:
1751 1096 662 432 421 353 297 42
EcoNI CCTnn'n_nnAGG
Cuts at: 803 1818 803
Size: 1015 4039
EcoO109I rG'GnC_Cy
Cuts at: 2193 2193
Size: 5054
EcoRI G'AATT_C
Cuts at: 483 926 1682 2058 483
Size: 443 756 376 3479
Fragments arranged by size:
3479 756 443 376
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3073 3073
Size: 5054
FspI TGC'GCA
Cuts at: 2835 2835
Size: 5054
HaeII r_GCGC'y
Cuts at: 2488 2736 3966 4737 2488
Size: 248 1230 771 2805
Fragments arranged by size:
2805 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 447 479 624 656 903 935 2896 2926 2928
Size: 32 145 32 247 32 1961 30 2
Cuts at: 2928 2958 447
Size: 30 2543
Fragments arranged by size:
2543 1961 247 145 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2093 2093
Size: 5054
KasI G'GCGC_C
Cuts at: 2484 2732 2484
Size: 248 4806
KpnI G_GTAC'C
Cuts at: 2074 2074
Size: 5054
MluI A'CGCG_T
Cuts at: 4487 4487
Size: 5054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 260 3813 3997 194
Size: 66 3553 184 1251
Fragments arranged by size:
3553 1251 184 66
MscI TGG'CCA
Cuts at: 2815 2815
Size: 5054
MslI CAynn'nnrTG
Cuts at: 238 962 1203 1271 1623 1875 1943 2041 3043
Size: 724 241 68 352 252 68 98 1002
Cuts at: 3043 3170 238
Size: 127 2122
Fragments arranged by size:
2122 1002 724 352 252 241 127 98
68 68
MspA1I CmG'CkG
Cuts at: 2165 2479 2839 3623 3868 2165
Size: 314 360 784 245 3351
Fragments arranged by size:
3351 784 360 314 245
NaeI GCC'GGC
Cuts at: 3235 3235
Size: 5054
NarI GG'CG_CC
Cuts at: 2485 2733 2485
Size: 248 4806
NcoI C'CATG_G
Cuts at: 107 298 107
Size: 191 4863
NdeI CA'TA_TG
Cuts at: 1200 1620 1200
Size: 420 4634
NgoMIV G'CCGG_C
Cuts at: 3233 3233
Size: 5054
NsiI A_TGCA'T
Cuts at: 975 975
Size: 5054
NspI r_CATG'y
Cuts at: 872 2107 2607 4210 872
Size: 1235 500 1603 1716
Fragments arranged by size:
1716 1603 1235 500
PciI A'CATG_T
Cuts at: 4206 4206
Size: 5054
PmeI GTTT'AAAC
Cuts at: 2053 2053
Size: 5054
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 5022
PsiI TTA'TAA
Cuts at: 1991 1991
Size: 5054
PstI C_TGCA'G
Cuts at: 898 2101 2786 898
Size: 1203 685 3166
Fragments arranged by size:
3166 1203 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5054
PvuII CAG'CTG
Cuts at: 2479 2839 2479
Size: 360 4694
RsrII CG'GwC_CG
Cuts at: 3249 3249
Size: 5054
SacI G_AGCT'C
Cuts at: 2068 2068
Size: 5054
SalI G'TCGA_C
Cuts at: 2091 2091
Size: 5054
SapI GCTCTTCn'nnn_
Cuts at: 287 834 1424 3077 3287 287
Size: 547 590 1653 210 2054
Fragments arranged by size:
2054 1653 590 547 210
SbfI CC_TGCA'GG
Cuts at: 2101 2101
Size: 5054
ScaI AGT'ACT
Cuts at: 621 4571 621
Size: 3950 1104
SfcI C'TryA_G
Cuts at: 33 894 2097 2782 3750 3941 33
Size: 861 1203 685 968 191 1146
Fragments arranged by size:
1203 1146 968 861 685 191
SfoI GGC'GCC
Cuts at: 2486 2734 2486
Size: 248 4806
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5054
SmaI CCC'GGG
Cuts at: 2076 2076
Size: 5054
SmlI C'TyrA_G
Cuts at: 274 1066 2209 3124 3838 4100 274
Size: 792 1143 915 714 262 1228
Fragments arranged by size:
1228 1143 915 792 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5054
SphI G_CATG'C
Cuts at: 2107 2607 2107
Size: 500 4554
SspI AAT'ATT
Cuts at: 852 852
Size: 5054
StuI AGG'CCT
Cuts at: 779 1561 779
Size: 782 4272
StyI C'CwwG_G
Cuts at: 107 298 2188 2542 107
Size: 191 1890 354 2619
Fragments arranged by size:
2619 1890 354 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2637 2770 3229 3438 2637
Size: 133 459 209 4253
Fragments arranged by size:
4253 459 209 133
TatI w'GTAC_w
Cuts at: 619 1335 1755 1923 4569 619
Size: 716 420 168 2646 1104
Fragments arranged by size:
2646 1104 716 420 168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4571 4603 4571
Size: 32 5022
Tth111I GACn'n_nGTC
Cuts at: 2851 2851
Size: 5054
XbaI T'CTAG_A
Cuts at: 391 2085 391
Size: 1694 3360
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2549 2549
Size: 5054
XmaI C'CCGG_G
Cuts at: 2074 2074
Size: 5054
XmnI GAAnn'nnTTC
Cuts at: 760 889 1141 1225 1393 760
Size: 129 252 84 168 4421
Fragments arranged by size:
4421 252 168 129 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI BsgI BsiWI BsmBI BspEI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI