Restriction map for
pF1KB4270
(Circular) (Six-base) MAPSORT of: pF1KB4270.seq Check: 7328 from: 1 to: 4370
pF1KB4270 4370 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1408 1408
Size: 4370
Acc65I G'GTAC_C
Cuts at: 1386 1386
Size: 4370
AclI AA'CG_TT
Cuts at: 4210 4210
Size: 4370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 968 2195 2627 2980 3642 331
Size: 637 1227 432 353 662 1059
Fragments arranged by size:
1227 1059 662 637 432 353
AflIII A'CryG_T
Cuts at: 488 1036 1722 3522 3803 488
Size: 548 686 1800 281 1055
Fragments arranged by size:
1800 1055 686 548 281
AlwNI CAG_nnn'CTG
Cuts at: 1675 3113 1675
Size: 1438 2932
ApaI G_GGCC'C
Cuts at: 1092 1092
Size: 4370
ApaLI G'TGCA_C
Cuts at: 3208 3208
Size: 4370
ApoI r'AATT_y
Cuts at: 559 1237 1374 3723 3979 559
Size: 678 137 2349 256 950
Fragments arranged by size:
2349 950 678 256 137
AseI AT'TA_AT
Cuts at: 20 20
Size: 4370
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
AvaI C'yCGr_G
Cuts at: 779 1390 779
Size: 611 3759
BamHI G'GATC_C
Cuts at: 932 1395 932
Size: 463 3907
BanI G'GyrC_C
Cuts at: 1386 1800 2048 2083 1386
Size: 414 248 35 3673
Fragments arranged by size:
3673 414 248 35
BanII G_rGCy'C
Cuts at: 786 1092 1384 2414 786
Size: 306 292 1030 2742
Fragments arranged by size:
2742 1030 306 292
BbeI G_GCGC'C
Cuts at: 1804 2052 1804
Size: 248 4122
BbsI GAAGACnn'nnnn_
Cuts at: 105 1065 105
Size: 960 3410
BbvCI CC'TCA_GC
Cuts at: 961 961
Size: 4370
BciVI GTATCCnnnnn_n'
Cuts at: 1310 2268 3324 4370 1310
Size: 958 1056 1046 1310
Fragments arranged by size:
1310 1056 1046 958
BclI T'GATC_A
Cuts at: 1890 1890
Size: 4370
BglII A'GATC_T
Cuts at: 1885 3922 1885
Size: 2037 2333
BlpI GC'TnA_GC
Cuts at: 1482 1482
Size: 4370
Bme1580I G_kGCm'C
Cuts at: 308 1092 1995 2088 3212 308
Size: 784 903 93 1124 1466
Fragments arranged by size:
1466 1124 903 784 93
BmrI ACTGGGnnnn_n'
Cuts at: 783 988 1582 1663 1998 4142 783
Size: 205 594 81 335 2144 1011
Fragments arranged by size:
2144 1011 594 335 205 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 4370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1245 1245
Size: 4370
Bpu10I CC'TnA_GC
Cuts at: 692 961 692
Size: 269 4101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 948 1177 1546 2425 3139 3437 948
Size: 229 369 879 714 298 1881
Fragments arranged by size:
1881 879 714 369 298 229
BsaI GGTCTCn'nnnn_
Cuts at: 988 993 4074 988
Size: 5 3081 1284
Fragments arranged by size:
3081 1284 5
BsaAI yAC'GTr
Cuts at: 1039 1723 1039
Size: 684 3686
BsaBI GATnn'nnATC
Cuts at: 6 1884 3921 6
Size: 1878 2037 455
Fragments arranged by size:
2037 1878 455
BsaHI Gr'CG_yC
Cuts at: 1801 2049 2751 4247 1801
Size: 248 702 1496 1924
Fragments arranged by size:
1924 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1559 2080 2758 3169 3316 3590 1559
Size: 521 678 411 147 274 2339
Fragments arranged by size:
2339 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 186 216 246 276 186
Size: 30 30 30 4280
Fragments arranged by size:
4280 30 30 30
BseYI C'CCAG_C
Cuts at: 1085 1795 2553 3218 3626 1085
Size: 710 758 665 408 1829
Fragments arranged by size:
1829 758 710 665 408
BsiEI CG_ry'CG
Cuts at: 104 1912 1958 3188 104
Size: 1808 46 1230 1286
Fragments arranged by size:
1808 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 786 1384 2162 2352 3212 786
Size: 598 778 190 860 1944
Fragments arranged by size:
1944 860 778 598 190
Bsp1286I G_dGCh'C
Cuts at: 308 786 1092 1384 1995 2088 2162 2352 2414
Size: 478 306 292 611 93 74 190 62
Cuts at: 2414 3212 308
Size: 798 1466
Fragments arranged by size:
1466 798 611 478 306 292 190 93
74 62
BspHI T'CATG_A
Cuts at: 986 2802 986
Size: 1816 2554
BspMI ACCTGCnnnn'nnnn_
Cuts at: 773 1420 1634 1936 2317 773
Size: 647 214 302 381 2826
Fragments arranged by size:
2826 647 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2662 2716 4200 4365 50
Size: 2612 54 1484 165 55
Fragments arranged by size:
2612 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 232 2282 232
Size: 2050 2320
BsrFI r'CCGG_y
Cuts at: 2368 2549 2368
Size: 181 4189
BssSI C'ACGA_G
Cuts at: 2641 3349 2641
Size: 708 3662
BstAPI GCAn_nnn'nTGC
Cuts at: 1747 1747
Size: 4370
BstBI TT'CG_AA
Cuts at: 2731 2731
Size: 4370
BstXI CCAn_nnnn'nTGG
Cuts at: 978 978
Size: 4370
BstYI r'GATC_y
Cuts at: 1 932 1395 1862 1885 2220 2466 2772 2784
Size: 931 463 467 23 335 246 306 12
Cuts at: 2784 2870 3922 1
Size: 86 1052 449
Fragments arranged by size:
1052 931 467 463 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 257 107
Size: 150 4220
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2320 2501 2664 2320
Size: 181 163 4026
Fragments arranged by size:
4026 181 163
BtsI GCAGTG_nn'
Cuts at: 128 506 1613 128
Size: 378 1107 2885
Fragments arranged by size:
2885 1107 378
DraI TTT'AAA
Cuts at: 1032 1369 1032
Size: 337 4033
DrdI GACnn_nn'nnGTC
Cuts at: 1319 2076 3420 1319
Size: 757 1344 2269
Fragments arranged by size:
2269 1344 757
EaeI y'GGCC_r
Cuts at: 1955 2129 2520 2547 3892 1955
Size: 174 391 27 1345 2433
Fragments arranged by size:
2433 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1955 1955
Size: 4370
EarI CTCTTCn'nnn_
Cuts at: 350 2393 2603 350
Size: 2043 210 2117
Fragments arranged by size:
2117 2043 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2578 3320 3466 4180 2578
Size: 742 146 714 2768
Fragments arranged by size:
2768 742 714 146
EcoICRI GAG'CTC
Cuts at: 784 1382 784
Size: 598 3772
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 968 1245 2195 2627 2980 3642 331
Size: 637 277 950 432 353 662 1059
Fragments arranged by size:
1059 950 662 637 432 353 277
EcoNI CCTnn'n_nnAGG
Cuts at: 964 964
Size: 4370
EcoO109I rG'GnC_Cy
Cuts at: 1089 1509 1089
Size: 420 3950
EcoRI G'AATT_C
Cuts at: 1374 1374
Size: 4370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2389 2389
Size: 4370
FspI TGC'GCA
Cuts at: 2151 2151
Size: 4370
HaeII r_GCGC'y
Cuts at: 1804 2052 3282 4053 1804
Size: 248 1230 771 2121
Fragments arranged by size:
2121 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 602 634 2212 2242 2244 2274 602
Size: 32 1578 30 2 30 2698
Fragments arranged by size:
2698 1578 32 30 30 2
HincII GTy'rAC
Cuts at: 128 1409 128
Size: 1281 3089
KasI G'GCGC_C
Cuts at: 1800 2048 1800
Size: 248 4122
KpnI G_GTAC'C
Cuts at: 1390 1390
Size: 4370
MluI A'CGCG_T
Cuts at: 3803 3803
Size: 4370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 176 3129 3313 176
Size: 2953 184 1233
Fragments arranged by size:
2953 1233 184
MscI TGG'CCA
Cuts at: 2131 2131
Size: 4370
MslI CAynn'nnrTG
Cuts at: 184 1021 2359 2486 184
Size: 837 1338 127 2068
Fragments arranged by size:
2068 1338 837 127
MspA1I CmG'CkG
Cuts at: 301 1212 1481 1795 2155 2939 3184 301
Size: 911 269 314 360 784 245 1487
Fragments arranged by size:
1487 911 784 360 314 269 245
NaeI GCC'GGC
Cuts at: 2551 2551
Size: 4370
NarI GG'CG_CC
Cuts at: 1801 2049 1801
Size: 248 4122
NcoI C'CATG_G
Cuts at: 107 257 107
Size: 150 4220
NgoMIV G'CCGG_C
Cuts at: 2549 2549
Size: 4370
NspI r_CATG'y
Cuts at: 492 513 1423 1923 3526 492
Size: 21 910 500 1603 1336
Fragments arranged by size:
1603 1336 910 500 21
PciI A'CATG_T
Cuts at: 488 3522 488
Size: 3034 1336
PmeI GTTT'AAAC
Cuts at: 1369 1369
Size: 4370
PmlI CAC'GTG
Cuts at: 1039 1039
Size: 4370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 680 712 2816 2848 680
Size: 32 2104 32 2202
Fragments arranged by size:
2202 2104 32 32
PshAI GACnn'nnGTC
Cuts at: 227 227
Size: 4370
PspOMI G'GGCC_C
Cuts at: 1088 1088
Size: 4370
PstI C_TGCA'G
Cuts at: 770 998 1417 2102 770
Size: 228 419 685 3038
Fragments arranged by size:
3038 685 419 228
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4370
PvuII CAG'CTG
Cuts at: 301 1795 2155 301
Size: 1494 360 2516
Fragments arranged by size:
2516 1494 360
RsrII CG'GwC_CG
Cuts at: 2565 2565
Size: 4370
SacI G_AGCT'C
Cuts at: 786 1384 786
Size: 598 3772
SalI G'TCGA_C
Cuts at: 1407 1407
Size: 4370
SapI GCTCTTCn'nnn_
Cuts at: 2393 2603 2393
Size: 210 4160
SbfI CC_TGCA'GG
Cuts at: 1417 1417
Size: 4370
ScaI AGT'ACT
Cuts at: 3887 3887
Size: 4370
SfcI C'TryA_G
Cuts at: 33 567 766 994 1413 2098 3066 3257 33
Size: 534 199 228 419 685 968 191 1146
Fragments arranged by size:
1146 968 685 534 419 228 199 191
SfoI GGC'GCC
Cuts at: 1802 2050 1802
Size: 248 4122
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
SmaI CCC'GGG
Cuts at: 1392 1392
Size: 4370
SmlI C'TyrA_G
Cuts at: 927 1192 1525 2440 3154 3416 927
Size: 265 333 915 714 262 1881
Fragments arranged by size:
1881 915 714 333 265 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4370
SphI G_CATG'C
Cuts at: 1423 1923 1423
Size: 500 3870
SspI AAT'ATT
Cuts at: 192 192
Size: 4370
StyI C'CwwG_G
Cuts at: 107 257 1504 1858 107
Size: 150 1247 354 2619
Fragments arranged by size:
2619 1247 354 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1953 2086 2545 2754 1953
Size: 133 459 209 3569
Fragments arranged by size:
3569 459 209 133
TatI w'GTAC_w
Cuts at: 295 884 3885 295
Size: 589 3001 780
Fragments arranged by size:
3001 780 589
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 798 830 3887 3919 798
Size: 32 3057 32 1249
Fragments arranged by size:
3057 1249 32 32
Tth111I GACn'n_nGTC
Cuts at: 2167 2167
Size: 4370
XbaI T'CTAG_A
Cuts at: 1401 1401
Size: 4370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 796 1865 796
Size: 1069 3301
XmaI C'CCGG_G
Cuts at: 1390 1390
Size: 4370
XmnI GAAnn'nnTTC
Cuts at: 562 562
Size: 4370
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PmlI PpiI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BseRI BsgI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PpuMI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI