Restriction map for
pF1KB4255
(Circular) (Six-base) MAPSORT of: pF1KB4255.seq Check: 8370 from: 1 to: 3848
pF1KB4255 3848 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 886 886
Size: 3848
Acc65I G'GTAC_C
Cuts at: 864 864
Size: 3848
AclI AA'CG_TT
Cuts at: 3688 3688
Size: 3848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 776 1673 2105 2852 3120 233
Size: 543 897 432 747 268 961
Fragments arranged by size:
961 897 747 543 432 268
AflIII A'CryG_T
Cuts at: 1200 2304 3281 1200
Size: 1104 977 1767
Fragments arranged by size:
1767 1104 977
AgeI A'CCGG_T
Cuts at: 2236 2236
Size: 3848
AlwNI CAG_nnn'CTG
Cuts at: 1153 2720 1153
Size: 1567 2281
ApaI G_GGCC'C
Cuts at: 436 436
Size: 3848
ApaLI G'TGCA_C
Cuts at: 500 2618 500
Size: 2118 1730
ApoI r'AATT_y
Cuts at: 118 852 3201 3457 118
Size: 734 2349 256 509
Fragments arranged by size:
2349 734 509 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3848
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
AvaI C'yCGr_G
Cuts at: 868 868
Size: 3848
BamHI G'GATC_C
Cuts at: 544 873 544
Size: 329 3519
BanI G'GyrC_C
Cuts at: 567 682 864 1278 1526 1561 567
Size: 115 182 414 248 35 2854
Fragments arranged by size:
2854 414 248 182 115 35
BanII G_rGCy'C
Cuts at: 436 862 1892 436
Size: 426 1030 2392
Fragments arranged by size:
2392 1030 426
BbeI G_GCGC'C
Cuts at: 571 1282 1530 571
Size: 711 248 2889
Fragments arranged by size:
2889 711 248
BbsI GAAGACnn'nnnn_
Cuts at: 545 545
Size: 3848
BciVI GTATCCnnnnn_n'
Cuts at: 332 599 752 1746 2507 3848 332
Size: 267 153 994 761 1341 332
Fragments arranged by size:
1341 994 761 332 267 153
BclI T'GATC_A
Cuts at: 1368 1368
Size: 3848
BglI GCCn_nnn'nGGC
Cuts at: 658 658
Size: 3848
BglII A'GATC_T
Cuts at: 1363 3400 1363
Size: 2037 1811
BlpI GC'TnA_GC
Cuts at: 960 960
Size: 3848
Bme1580I G_kGCm'C
Cuts at: 436 504 685 1473 1566 2622 436
Size: 68 181 788 93 1056 1662
Fragments arranged by size:
1662 1056 788 181 93 68
BmrI ACTGGGnnnn_n'
Cuts at: 764 1060 1141 1476 3620 764
Size: 296 81 335 2144 992
Fragments arranged by size:
2144 992 335 296 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 3848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 602 335
Size: 267 3581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 1024 1903 2395 2693 185
Size: 839 879 492 298 1340
Fragments arranged by size:
1340 879 839 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3552 3552
Size: 3848
BsaAI yAC'GTr
Cuts at: 1201 1201
Size: 3848
BsaBI GATnn'nnATC
Cuts at: 6 1362 3399 6
Size: 1356 2037 455
Fragments arranged by size:
2037 1356 455
BsaHI Gr'CG_yC
Cuts at: 568 1279 1527 2229 3725 568
Size: 711 248 702 1496 691
Fragments arranged by size:
1496 711 702 691 248
BsaWI w'CCGG_w
Cuts at: 340 379 1037 1558 2236 2510 2657 3068 340
Size: 39 658 521 678 274 147 411 1120
Fragments arranged by size:
1120 678 658 521 411 274 147 39
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 188 626 629 632 188
Size: 438 3 3 3404
Fragments arranged by size:
3404 438 3 3
BseYI C'CCAG_C
Cuts at: 360 1273 2031 2608 3104 360
Size: 913 758 577 496 1104
Fragments arranged by size:
1104 913 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1390 1436 2644 104
Size: 1286 46 1208 1308
Fragments arranged by size:
1308 1286 1208 46
BsiHKAI G_wGCw'C
Cuts at: 243 504 810 862 1640 1830 2622 243
Size: 261 306 52 778 190 792 1469
Fragments arranged by size:
1469 792 778 306 261 190 52
Bsp1286I G_dGCh'C
Cuts at: 243 436 504 685 810 862 1473 1566 1640
Size: 193 68 181 125 52 611 93 74
Cuts at: 1640 1830 1892 2622 243
Size: 190 62 730 1469
Fragments arranged by size:
1469 730 611 193 190 181 125 93
74 68 62 52
BspEI T'CCGG_A
Cuts at: 379 3068 379
Size: 2689 1159
BspHI T'CATG_A
Cuts at: 3024 3024
Size: 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 898 1112 1414 1795 898
Size: 214 302 381 2951
Fragments arranged by size:
2951 381 302 214
BsrBI CCG'CTC
Cuts at: 50 634 2140 2194 3678 3843 50
Size: 584 1506 54 1484 165 55
Fragments arranged by size:
1506 1484 584 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1760 1760
Size: 3848
BsrFI r'CCGG_y
Cuts at: 1846 2027 2236 1846
Size: 181 209 3458
Fragments arranged by size:
3458 209 181
BssSI C'ACGA_G
Cuts at: 2119 2477 2119
Size: 358 3490
BstAPI GCAn_nnn'nTGC
Cuts at: 1225 1225
Size: 3848
BstBI TT'CG_AA
Cuts at: 2209 2209
Size: 3848
BstXI CCAn_nnnn'nTGG
Cuts at: 655 701 655
Size: 46 3802
BstYI r'GATC_y
Cuts at: 1 510 544 873 1340 1363 1698 1944 2956
Size: 509 34 329 467 23 335 246 1012
Cuts at: 2956 3042 3054 3400 1
Size: 86 12 346 449
Fragments arranged by size:
1012 509 467 449 346 335 329 246
86 34 23 12
BtgI C'CryG_G
Cuts at: 538 676 538
Size: 138 3710
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1798 1979 2142 1798
Size: 181 163 3504
Fragments arranged by size:
3504 181 163
BtsI GCAGTG_nn'
Cuts at: 1091 1091
Size: 3848
DraI TTT'AAA
Cuts at: 847 847
Size: 3848
DraIII CAC_nnn'GTG
Cuts at: 274 403 274
Size: 129 3719
DrdI GACnn_nn'nnGTC
Cuts at: 389 1554 2412 389
Size: 1165 858 1825
Fragments arranged by size:
1825 1165 858
EaeI y'GGCC_r
Cuts at: 189 208 1433 1607 1998 2025 3370 189
Size: 19 1225 174 391 27 1345 667
Fragments arranged by size:
1345 1225 667 391 174 27 19
EagI C'GGCC_G
Cuts at: 1433 1433
Size: 3848
EarI CTCTTCn'nnn_
Cuts at: 1871 2081 1871
Size: 210 3638
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2056 2366 2512 3658 2056
Size: 310 146 1146 2246
Fragments arranged by size:
2246 1146 310 146
EcoICRI GAG'CTC
Cuts at: 860 860
Size: 3848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 335 602 776 1673 2105 2852 3120 233
Size: 102 267 174 897 432 747 268 961
Fragments arranged by size:
961 897 747 432 268 267 174 102
EcoNI CCTnn'n_nnAGG
Cuts at: 200 200
Size: 3848
EcoO109I rG'GnC_Cy
Cuts at: 355 433 796 987 355
Size: 78 363 191 3216
Fragments arranged by size:
3216 363 191 78
EcoRI G'AATT_C
Cuts at: 852 852
Size: 3848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1867 1867
Size: 3848
FspI TGC'GCA
Cuts at: 1629 1629
Size: 3848
HaeII r_GCGC'y
Cuts at: 571 1282 1530 2552 3531 571
Size: 711 248 1022 979 888
Fragments arranged by size:
1022 979 888 711 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1690 1720 1722 1752 1690
Size: 30 2 30 3786
Fragments arranged by size:
3786 30 30 2
HincII GTy'rAC
Cuts at: 887 887
Size: 3848
KasI G'GCGC_C
Cuts at: 567 1278 1526 567
Size: 711 248 2889
Fragments arranged by size:
2889 711 248
KpnI G_GTAC'C
Cuts at: 868 868
Size: 3848
MluI A'CGCG_T
Cuts at: 3281 3281
Size: 3848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 242 2519 2703 242
Size: 2277 184 1387
Fragments arranged by size:
2277 1387 184
MscI TGG'CCA
Cuts at: 191 210 1609 191
Size: 19 1399 2430
Fragments arranged by size:
2430 1399 19
MslI CAynn'nnrTG
Cuts at: 1837 1964 1837
Size: 127 3721
MspA1I CmG'CkG
Cuts at: 517 523 959 1273 1633 2646 2891 517
Size: 6 436 314 360 1013 245 1474
Fragments arranged by size:
1474 1013 436 360 314 245 6
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 3848
NarI GG'CG_CC
Cuts at: 568 1279 1527 568
Size: 711 248 2889
Fragments arranged by size:
2889 711 248
NcoI C'CATG_G
Cuts at: 538 676 538
Size: 138 3710
NdeI CA'TA_TG
Cuts at: 495 495
Size: 3848
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 3848
NspI r_CATG'y
Cuts at: 901 1401 2308 901
Size: 500 907 2441
Fragments arranged by size:
2441 907 500
PasI CC'CwG_GG
Cuts at: 655 655
Size: 3848
PciI A'CATG_T
Cuts at: 2304 2304
Size: 3848
PflMI CCAn_nnn'nTGG
Cuts at: 224 403 820 224
Size: 179 417 3252
Fragments arranged by size:
3252 417 179
PfoI T'CCnGG_A
Cuts at: 351 351
Size: 3848
PmeI GTTT'AAAC
Cuts at: 847 847
Size: 3848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 3816
PpuMI rG'GwC_Cy
Cuts at: 355 796 355
Size: 441 3407
PspOMI G'GGCC_C
Cuts at: 432 432
Size: 3848
PstI C_TGCA'G
Cuts at: 522 895 1580 522
Size: 373 685 2790
Fragments arranged by size:
2790 685 373
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3848
PvuII CAG'CTG
Cuts at: 517 523 1273 1633 517
Size: 6 750 360 2732
Fragments arranged by size:
2732 750 360 6
RsrII CG'GwC_CG
Cuts at: 2043 2043
Size: 3848
SacI G_AGCT'C
Cuts at: 862 862
Size: 3848
SalI G'TCGA_C
Cuts at: 885 885
Size: 3848
SapI GCTCTTCn'nnn_
Cuts at: 1871 2081 1871
Size: 210 3638
SbfI CC_TGCA'GG
Cuts at: 895 895
Size: 3848
ScaI AGT'ACT
Cuts at: 3365 3365
Size: 3848
SexAI A'CCwGG_T
Cuts at: 399 399
Size: 3848
SfcI C'TryA_G
Cuts at: 33 518 731 891 1576 2569 2760 33
Size: 485 213 160 685 993 191 1121
Fragments arranged by size:
1121 993 685 485 213 191 160
SfoI GGC'GCC
Cuts at: 569 1280 1528 569
Size: 711 248 2889
Fragments arranged by size:
2889 711 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
SmaI CCC'GGG
Cuts at: 870 870
Size: 3848
SmlI C'TyrA_G
Cuts at: 200 1003 1918 2410 2672 200
Size: 803 915 492 262 1376
Fragments arranged by size:
1376 915 803 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3848
SphI G_CATG'C
Cuts at: 901 1401 901
Size: 500 3348
StyI C'CwwG_G
Cuts at: 223 538 562 676 982 1336 223
Size: 315 24 114 306 354 2735
Fragments arranged by size:
2735 354 315 306 114 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 519 1431 1564 2023 2232 519
Size: 912 133 459 209 2135
Fragments arranged by size:
2135 912 459 209 133
TatI w'GTAC_w
Cuts at: 3363 3363
Size: 3848
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 3816
Tth111I GACn'n_nGTC
Cuts at: 1645 1645
Size: 3848
XbaI T'CTAG_A
Cuts at: 879 879
Size: 3848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 674 698 1343 674
Size: 24 645 3179
Fragments arranged by size:
3179 645 24
XmaI C'CCGG_G
Cuts at: 868 868
Size: 3848
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI