Restriction map for
pF1KB4251
(Circular) (Six-base) MAPSORT of: pF1KB4251.seq Check: 1362 from: 1 to: 6032
pF1KB4251 6032 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 246 577 246
Size: 331 5701
AccI GT'mk_AC
Cuts at: 2523 3070 2523
Size: 547 5485
Acc65I G'GTAC_C
Cuts at: 3048 3048
Size: 6032
AclI AA'CG_TT
Cuts at: 5872 5872
Size: 6032
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 1623 2248 2491 2593 3857 4289 4642 5304
Size: 1011 625 243 102 1264 432 353 662
Cuts at: 5304 612
Size: 1340
Fragments arranged by size:
1340 1264 1011 662 625 432 353 243
102
AflIII A'CryG_T
Cuts at: 1803 1976 2894 3384 5184 5465 1803
Size: 173 918 490 1800 281 2370
Fragments arranged by size:
2370 1800 918 490 281 173
AleI CACnn'nnGTG
Cuts at: 1694 1694
Size: 6032
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1739 1739
Size: 6032
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2981 3013 2981
Size: 32 6000
AlwNI CAG_nnn'CTG
Cuts at: 2874 3337 4775 2874
Size: 463 1438 4131
Fragments arranged by size:
4131 1438 463
ApaI G_GGCC'C
Cuts at: 2134 2134
Size: 6032
ApaLI G'TGCA_C
Cuts at: 4870 4870
Size: 6032
ApoI r'AATT_y
Cuts at: 3036 5385 5641 3036
Size: 2349 256 3427
Fragments arranged by size:
3427 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6032
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6032
AvaI C'yCGr_G
Cuts at: 641 1961 2426 3052 641
Size: 1320 465 626 3621
Fragments arranged by size:
3621 1320 626 465
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1365 1398 1365
Size: 33 5999
BamHI G'GATC_C
Cuts at: 111 471 3057 111
Size: 360 2586 3086
Fragments arranged by size:
3086 2586 360
BanI G'GyrC_C
Cuts at: 2914 3048 3462 3710 3745 2914
Size: 134 414 248 35 5201
Fragments arranged by size:
5201 414 248 134 35
BanII G_rGCy'C
Cuts at: 1049 1229 1681 1769 2020 2134 2294 2491 2857
Size: 180 452 88 251 114 160 197 366
Cuts at: 2857 3046 4076 1049
Size: 189 1030 3005
Fragments arranged by size:
3005 1030 452 366 251 197 189 180
160 114 88
BbeI G_GCGC'C
Cuts at: 3466 3714 3466
Size: 248 5784
BbsI GAAGACnn'nnnn_
Cuts at: 2581 2581
Size: 6032
BbvCI CC'TCA_GC
Cuts at: 2093 2093
Size: 6032
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 861 895 861
Size: 34 5998
BciVI GTATCCnnnnn_n'
Cuts at: 3930 4986 6032 3930
Size: 1056 1046 3930
Fragments arranged by size:
3930 1056 1046
BclI T'GATC_A
Cuts at: 2859 3552 2859
Size: 693 5339
BfrBI ATG'CAT
Cuts at: 1290 1917 1290
Size: 627 5405
BglI GCCn_nnn'nGGC
Cuts at: 2003 2232 2486 2003
Size: 229 254 5549
Fragments arranged by size:
5549 254 229
BglII A'GATC_T
Cuts at: 1181 3547 5584 1181
Size: 2366 2037 1629
Fragments arranged by size:
2366 2037 1629
BlpI GC'TnA_GC
Cuts at: 1661 3144 1661
Size: 1483 4549
Bme1580I G_kGCm'C
Cuts at: 1929 2134 2192 3657 3750 4874 1929
Size: 205 58 1465 93 1124 3087
Fragments arranged by size:
3087 1465 1124 205 93 58
BmgBI CAC'GTC
Cuts at: 1804 1804
Size: 6032
BmrI ACTGGGnnnn_n'
Cuts at: 153 583 675 1529 1878 2179 2351 3244 3325
Size: 430 92 854 349 301 172 893 81
Cuts at: 3325 3660 5804 153
Size: 335 2144 381
Fragments arranged by size:
2144 893 854 430 381 349 335 301
172 92 81
BmtI G_CTAG'C
Cuts at: 1205 1240 2959 1205
Size: 35 1719 4278
Fragments arranged by size:
4278 1719 35
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 471 1068 4399 471
Size: 597 3331 2104
Fragments arranged by size:
3331 2104 597
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 683 1063 683
Size: 380 5652
Bpu10I CC'TnA_GC
Cuts at: 1592 2093 2851 1592
Size: 501 758 4773
Fragments arranged by size:
4773 758 501
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 3208 4087 4801 5099 994
Size: 2214 879 714 298 1927
Fragments arranged by size:
2214 1927 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 327 505 1883 5736 327
Size: 178 1378 3853 623
Fragments arranged by size:
3853 1378 623 178
BsaAI yAC'GTr
Cuts at: 2137 3385 2137
Size: 1248 4784
BsaBI GATnn'nnATC
Cuts at: 6 2774 3546 5583 6
Size: 2768 772 2037 455
Fragments arranged by size:
2768 2037 772 455
BsaHI Gr'CG_yC
Cuts at: 3463 3711 4413 5909 3463
Size: 248 702 1496 3586
Fragments arranged by size:
3586 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1609 3221 3742 4420 4831 4978 5252 1609
Size: 1612 521 678 411 147 274 2389
Fragments arranged by size:
2389 1612 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2706 2736 2706
Size: 30 6002
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2690 2690
Size: 6032
BseYI C'CCAG_C
Cuts at: 216 490 3457 4215 4880 5288 216
Size: 274 2967 758 665 408 960
Fragments arranged by size:
2967 960 758 665 408 274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 2197 676
Size: 1521 4511
BsiEI CG_ry'CG
Cuts at: 104 3574 3620 4850 104
Size: 3470 46 1230 1286
Fragments arranged by size:
3470 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 237 2294 3046 3824 4014 4874 237
Size: 2057 752 778 190 860 1395
Fragments arranged by size:
2057 1395 860 778 752 190
BsmI GAATG_Cn'
Cuts at: 1064 1711 2810 1064
Size: 647 1099 4286
Fragments arranged by size:
4286 1099 647
Bsp1286I G_dGCh'C
Cuts at: 237 1049 1229 1681 1769 1929 2020 2134 2192
Size: 812 180 452 88 160 91 114 58
Cuts at: 2192 2294 2491 2857 3046 3657 3750 3824 4014
Size: 102 197 366 189 611 93 74 190
Cuts at: 4014 4076 4874 237
Size: 62 798 1395
Fragments arranged by size:
1395 812 798 611 452 366 197 190
189 180 160 114 102 93 91 88
74 62 58
BspHI T'CATG_A
Cuts at: 2985 4464 2985
Size: 1479 4553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 246 577 3082 3296 3598 3979 246
Size: 331 2505 214 302 381 2299
Fragments arranged by size:
2505 2299 381 331 302 214
BsrBI CCG'CTC
Cuts at: 50 4324 4378 5862 6027 50
Size: 4274 54 1484 165 55
Fragments arranged by size:
4274 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1516 1955 3944 1516
Size: 439 1989 3604
Fragments arranged by size:
3604 1989 439
BsrFI r'CCGG_y
Cuts at: 910 1993 2022 4030 4211 910
Size: 1083 29 2008 181 2731
Fragments arranged by size:
2731 2008 1083 181 29
BsrGI T'GTAC_A
Cuts at: 1785 1785
Size: 6032
BssSI C'ACGA_G
Cuts at: 2482 4303 5011 2482
Size: 1821 708 3503
Fragments arranged by size:
3503 1821 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3409 3409
Size: 6032
BstBI TT'CG_AA
Cuts at: 2211 4393 2211
Size: 2182 3850
BstXI CCAn_nnnn'nTGG
Cuts at: 188 259 2027 2649 188
Size: 71 1768 622 3571
Fragments arranged by size:
3571 1768 622 71
BstYI r'GATC_y
Cuts at: 1 111 471 934 1037 1181 3057 3524 3547
Size: 110 360 463 103 144 1876 467 23
Cuts at: 3547 3882 4128 4434 4446 4532 5584 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1876 1052 467 463 449 360 335 306
246 144 110 103 86 23 12
BtgI C'CryG_G
Cuts at: 107 2375 107
Size: 2268 3764
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3982 4163 4326 3982
Size: 181 163 5688
Fragments arranged by size:
5688 181 163
BtsI GCAGTG_nn'
Cuts at: 920 3275 920
Size: 2355 3677
DraI TTT'AAA
Cuts at: 3031 3031
Size: 6032
DrdI GACnn_nn'nnGTC
Cuts at: 3738 5082 3738
Size: 1344 4688
EaeI y'GGCC_r
Cuts at: 190 1186 2565 3617 3791 4182 4209 5554 190
Size: 996 1379 1052 174 391 27 1345 668
Fragments arranged by size:
1379 1345 1052 996 668 391 174 27
EagI C'GGCC_G
Cuts at: 3617 3617
Size: 6032
EarI CTCTTCn'nnn_
Cuts at: 537 573 1234 1499 4055 4265 537
Size: 36 661 265 2556 210 2304
Fragments arranged by size:
2556 2304 661 265 210 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4240 4982 5128 5842 4240
Size: 742 146 714 4430
Fragments arranged by size:
4430 742 714 146
EcoICRI GAG'CTC
Cuts at: 2292 3044 2292
Size: 752 5280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 683 1063 1623 2248 2491 2593 3857 4289
Size: 71 380 560 625 243 102 1264 432
Cuts at: 4289 4642 5304 612
Size: 353 662 1340
Fragments arranged by size:
1340 1264 662 625 560 432 380 353
243 102 71
EcoO109I rG'GnC_Cy
Cuts at: 646 3171 646
Size: 2525 3507
EcoRI G'AATT_C
Cuts at: 3036 3036
Size: 6032
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1446 1653 4051 1446
Size: 207 2398 3427
Fragments arranged by size:
3427 2398 207
FspI TGC'GCA
Cuts at: 3813 3813
Size: 6032
HaeII r_GCGC'y
Cuts at: 3466 3714 4944 5715 3466
Size: 248 1230 771 3783
Fragments arranged by size:
3783 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 779 811 926 958 1166 1198 1955 1987 2433
Size: 32 115 32 208 32 757 32 446
Cuts at: 2433 2465 2542 2574 2844 2876 3874 3904 3906
Size: 32 77 32 270 32 998 30 2
Cuts at: 3906 3936 779
Size: 30 2875
Fragments arranged by size:
2875 998 757 446 270 208 115 77
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1974 3071 1974
Size: 1097 4935
HindIII A'AGCT_T
Cuts at: 197 958 1117 1339 2621 197
Size: 761 159 222 1282 3608
Fragments arranged by size:
3608 1282 761 222 159
KasI G'GCGC_C
Cuts at: 3462 3710 3462
Size: 248 5784
KpnI G_GTAC'C
Cuts at: 3052 3052
Size: 6032
MluI A'CGCG_T
Cuts at: 5465 5465
Size: 6032
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 144 505 1961 4791 4975 144
Size: 361 1456 2830 184 1201
Fragments arranged by size:
2830 1456 1201 361 184
MscI TGG'CCA
Cuts at: 192 1188 2567 3793 192
Size: 996 1379 1226 2431
Fragments arranged by size:
2431 1379 1226 996
MslI CAynn'nnrTG
Cuts at: 159 1694 2005 2011 2125 4021 4148 159
Size: 1535 311 6 114 1896 127 2043
Fragments arranged by size:
2043 1896 1535 311 127 114 6
MspA1I CmG'CkG
Cuts at: 657 3003 3143 3457 3817 4601 4846 657
Size: 2346 140 314 360 784 245 1843
Fragments arranged by size:
2346 1843 784 360 314 245 140
NaeI GCC'GGC
Cuts at: 1995 4213 1995
Size: 2218 3814
NarI GG'CG_CC
Cuts at: 3463 3711 3463
Size: 248 5784
NcoI C'CATG_G
Cuts at: 107 107
Size: 6032
NdeI CA'TA_TG
Cuts at: 2864 2864
Size: 6032
NgoMIV G'CCGG_C
Cuts at: 1993 4211 1993
Size: 2218 3814
NheI G'CTAG_C
Cuts at: 1201 1236 2955 1201
Size: 35 1719 4278
Fragments arranged by size:
4278 1719 35
NsiI A_TGCA'T
Cuts at: 1292 1919 1292
Size: 627 5405
NspI r_CATG'y
Cuts at: 888 1294 1980 2010 2370 2898 3085 3585 5188
Size: 406 686 30 360 528 187 500 1603
Cuts at: 5188 888
Size: 1732
Fragments arranged by size:
1732 1603 686 528 500 406 360 187
30
PciI A'CATG_T
Cuts at: 1976 2894 5184 1976
Size: 918 2290 2824
Fragments arranged by size:
2824 2290 918
PflMI CCAn_nnn'nTGG
Cuts at: 467 467
Size: 6032
PfoI T'CCnGG_A
Cuts at: 1040 1322 2724 1040
Size: 282 1402 4348
Fragments arranged by size:
4348 1402 282
PmeI GTTT'AAAC
Cuts at: 3031 3031
Size: 6032
PmlI CAC'GTG
Cuts at: 2137 2137
Size: 6032
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3001 3033 4478 4510 3001
Size: 32 1445 32 4523
Fragments arranged by size:
4523 1445 32 32
PpuMI rG'GwC_Cy
Cuts at: 646 646
Size: 6032
PshAI GACnn'nnGTC
Cuts at: 2062 2062
Size: 6032
PspOMI G'GGCC_C
Cuts at: 2130 2130
Size: 6032
PstI C_TGCA'G
Cuts at: 243 3079 3764 243
Size: 2836 685 2511
Fragments arranged by size:
2836 2511 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6032
PvuII CAG'CTG
Cuts at: 657 3003 3457 3817 657
Size: 2346 454 360 2872
Fragments arranged by size:
2872 2346 454 360
RsrII CG'GwC_CG
Cuts at: 4227 4227
Size: 6032
SacI G_AGCT'C
Cuts at: 2294 3046 2294
Size: 752 5280
SalI G'TCGA_C
Cuts at: 3069 3069
Size: 6032
SanDI GG'GwC_CC
Cuts at: 646 646
Size: 6032
SapI GCTCTTCn'nnn_
Cuts at: 573 1234 4055 4265 573
Size: 661 2821 210 2340
Fragments arranged by size:
2821 2340 661 210
SbfI CC_TGCA'GG
Cuts at: 243 3079 243
Size: 2836 3196
ScaI AGT'ACT
Cuts at: 2679 5549 2679
Size: 2870 3162
SfcI C'TryA_G
Cuts at: 33 239 1162 1473 2605 3075 3760 4728 4919
Size: 206 923 311 1132 470 685 968 191
Cuts at: 4919 33
Size: 1146
Fragments arranged by size:
1146 1132 968 923 685 470 311 206
191
SfoI GGC'GCC
Cuts at: 3464 3712 3464
Size: 248 5784
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6032
SmaI CCC'GGG
Cuts at: 643 2428 3054 643
Size: 1785 626 3621
Fragments arranged by size:
3621 1785 626
SmlI C'TyrA_G
Cuts at: 973 1961 3187 4102 4816 5078 973
Size: 988 1226 915 714 262 1927
Fragments arranged by size:
1927 1226 988 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6032
SphI G_CATG'C
Cuts at: 1294 2010 2370 3085 3585 1294
Size: 716 360 715 500 3741
Fragments arranged by size:
3741 716 715 500 360
SspI AAT'ATT
Cuts at: 1356 1356
Size: 6032
StuI AGG'CCT
Cuts at: 338 338
Size: 6032
StyI C'CwwG_G
Cuts at: 107 3166 3520 107
Size: 3059 354 2619
Fragments arranged by size:
3059 2619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 167 2063 2272 2754 3615 3748 4207 4416 167
Size: 1896 209 482 861 133 459 209 1783
Fragments arranged by size:
1896 1783 861 482 459 209 209 133
TatI w'GTAC_w
Cuts at: 1333 1785 2677 2897 5547 1333
Size: 452 892 220 2650 1818
Fragments arranged by size:
2650 1818 892 452 220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1257 1289 5549 5581 1257
Size: 32 4260 32 1708
Fragments arranged by size:
4260 1708 32 32
Tth111I GACn'n_nGTC
Cuts at: 3829 3829
Size: 6032
XbaI T'CTAG_A
Cuts at: 3063 3063
Size: 6032
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1653 3527 1653
Size: 1874 4158
XhoI C'TCGA_G
Cuts at: 1961 1961
Size: 6032
XmaI C'CCGG_G
Cuts at: 641 2426 3052 641
Size: 1785 626 3621
Fragments arranged by size:
3621 1785 626
XmnI GAAnn'nnTTC
Cuts at: 383 1636 3016 383
Size: 1253 1380 3399
Fragments arranged by size:
3399 1380 1253
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AscI AvrII BsiWI
BsmBI BspEI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NotI
NruI PacI PasI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SwaI ZraI