Restriction map for
pF1KB4237
(Circular) (Six-base) MAPSORT of: pF1KB4237.seq Check: 3379 from: 1 to: 5564
pF1KB4237 5564 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 453 535 453
Size: 82 5482
AccI GT'mk_AC
Cuts at: 2602 2602
Size: 5564
Acc65I G'GTAC_C
Cuts at: 2580 2580
Size: 5564
AclI AA'CG_TT
Cuts at: 5404 5404
Size: 5564
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 736 1021 1529 3389 3821 4568 4836 519
Size: 217 285 508 1860 432 747 268 1247
Fragments arranged by size:
1860 1247 747 508 432 285 268 217
AflIII A'CryG_T
Cuts at: 677 1064 2916 4020 4997 677
Size: 387 1852 1104 977 1244
Fragments arranged by size:
1852 1244 1104 977 387
AgeI A'CCGG_T
Cuts at: 3952 3952
Size: 5564
AleI CACnn'nnGTG
Cuts at: 1489 1489
Size: 5564
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2161 2193 2161
Size: 32 5532
AlwNI CAG_nnn'CTG
Cuts at: 730 2869 4436 730
Size: 2139 1567 1858
Fragments arranged by size:
2139 1858 1567
ApaI G_GGCC'C
Cuts at: 806 1632 2011 806
Size: 826 379 4359
Fragments arranged by size:
4359 826 379
ApaLI G'TGCA_C
Cuts at: 432 4334 432
Size: 3902 1662
ApoI r'AATT_y
Cuts at: 1976 2047 2059 2434 2568 4917 5173 1976
Size: 71 12 375 134 2349 256 2367
Fragments arranged by size:
2367 2349 375 256 134 71 12
AseI AT'TA_AT
Cuts at: 20 1715 20
Size: 1695 3869
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5564
AvaI C'yCGr_G
Cuts at: 181 378 769 1147 1652 1887 2141 2584 181
Size: 197 391 378 505 235 254 443 3161
Fragments arranged by size:
3161 505 443 391 378 254 235 197
BamHI G'GATC_C
Cuts at: 2065 2589 2065
Size: 524 5040
BanI G'GyrC_C
Cuts at: 176 244 360 2580 2994 3242 3277 176
Size: 68 116 2220 414 248 35 2463
Fragments arranged by size:
2463 2220 414 248 116 68 35
BanII G_rGCy'C
Cuts at: 806 1579 1632 1682 1827 2011 2578 3608 806
Size: 773 53 50 145 184 567 1030 2762
Fragments arranged by size:
2762 1030 773 567 184 145 53 50
BbeI G_GCGC'C
Cuts at: 180 248 2998 3246 180
Size: 68 2750 248 2498
Fragments arranged by size:
2750 2498 248 68
BbvCI CC'TCA_GC
Cuts at: 1235 1235
Size: 5564
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5530
BciVI GTATCCnnnnn_n'
Cuts at: 3462 4223 5564 3462
Size: 761 1341 3462
Fragments arranged by size:
3462 1341 761
BclI T'GATC_A
Cuts at: 1997 3084 1997
Size: 1087 4477
BglII A'GATC_T
Cuts at: 2402 3079 5116 2402
Size: 677 2037 2850
Fragments arranged by size:
2850 2037 677
BlpI GC'TnA_GC
Cuts at: 1681 2676 1681
Size: 995 4569
Bme1580I G_kGCm'C
Cuts at: 139 436 806 1632 2011 2528 3189 3282 4338
Size: 297 370 826 379 517 661 93 1056
Cuts at: 4338 139
Size: 1365
Fragments arranged by size:
1365 1056 826 661 517 379 370 297
93
BmgBI CAC'GTC
Cuts at: 678 2098 678
Size: 1420 4144
BmrI ACTGGGnnnn_n'
Cuts at: 1491 1787 2776 2857 3192 5336 1491
Size: 296 989 81 335 2144 1719
Fragments arranged by size:
2144 1719 989 335 296 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3931 3931
Size: 5564
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2446 2446
Size: 5564
Bpu10I CC'TnA_GC
Cuts at: 368 1235 368
Size: 867 4697
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 2740 3619 4111 4409 529
Size: 2211 879 492 298 1684
Fragments arranged by size:
2211 1684 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 766 1388 5268 766
Size: 622 3880 1062
Fragments arranged by size:
3880 1062 622
BsaAI yAC'GTr
Cuts at: 2917 2917
Size: 5564
BsaBI GATnn'nnATC
Cuts at: 6 1643 3078 5115 6
Size: 1637 1435 2037 455
Fragments arranged by size:
2037 1637 1435 455
BsaHI Gr'CG_yC
Cuts at: 177 245 918 2995 3243 3945 5441 177
Size: 68 673 2077 248 702 1496 300
Fragments arranged by size:
2077 1496 702 673 300 248 68
BsaWI w'CCGG_w
Cuts at: 1397 2753 3274 3952 4226 4373 4784 1397
Size: 1356 521 678 274 147 411 2177
Fragments arranged by size:
2177 1356 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1267 1297 2026 2056 1267
Size: 30 729 30 4775
Fragments arranged by size:
4775 729 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1172 1175 1252 1328 1172
Size: 3 77 76 5408
Fragments arranged by size:
5408 77 76 3
BseYI C'CCAG_C
Cuts at: 415 636 829 1362 2989 3747 4324 4820 415
Size: 221 193 533 1627 758 577 496 1159
Fragments arranged by size:
1627 1159 758 577 533 496 221 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1258 1258
Size: 5564
BsiEI CG_ry'CG
Cuts at: 104 345 3106 3152 4360 104
Size: 241 2761 46 1208 1308
Fragments arranged by size:
2761 1308 1208 241 46
BsiHKAI G_wGCw'C
Cuts at: 391 436 1579 1827 2578 3356 3546 4338 391
Size: 45 1143 248 751 778 190 792 1617
Fragments arranged by size:
1617 1143 792 778 751 248 190 45
BsmI GAATG_Cn'
Cuts at: 1437 1437
Size: 5564
Bsp1286I G_dGCh'C
Cuts at: 139 391 436 806 1579 1632 1682 1827 2011
Size: 252 45 370 773 53 50 145 184
Cuts at: 2011 2528 2578 3189 3282 3356 3546 3608 4338
Size: 517 50 611 93 74 190 62 730
Cuts at: 4338 139
Size: 1365
Fragments arranged by size:
1365 773 730 611 517 370 252 190
184 145 93 74 62 53 50 50
45
BspEI T'CCGG_A
Cuts at: 4784 4784
Size: 5564
BspHI T'CATG_A
Cuts at: 1440 4740 1440
Size: 3300 2264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 453 535 800 2614 2828 3130 3511 453
Size: 82 265 1814 214 302 381 2506
Fragments arranged by size:
2506 1814 381 302 265 214 82
BsrBI CCG'CTC
Cuts at: 50 3856 3910 5394 5559 50
Size: 3806 54 1484 165 55
Fragments arranged by size:
3806 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 824 2289 2321 3476 824
Size: 1465 32 1155 2912
Fragments arranged by size:
2912 1465 1155 32
BsrFI r'CCGG_y
Cuts at: 340 357 935 1471 3562 3743 3952 340
Size: 17 578 536 2091 181 209 1952
Fragments arranged by size:
2091 1952 578 536 209 181 17
BsrGI T'GTAC_A
Cuts at: 2460 2460
Size: 5564
BssSI C'ACGA_G
Cuts at: 979 3835 4193 979
Size: 2856 358 2350
Fragments arranged by size:
2856 2350 358
BstAPI GCAn_nnn'nTGC
Cuts at: 282 2261 2941 282
Size: 1979 680 2905
Fragments arranged by size:
2905 1979 680
BstBI TT'CG_AA
Cuts at: 3925 3925
Size: 5564
BstEII G'GTnAC_C
Cuts at: 153 1752 153
Size: 1599 3965
BstXI CCAn_nnnn'nTGG
Cuts at: 813 1293 2424 813
Size: 480 1131 3953
Fragments arranged by size:
3953 1131 480
BstYI r'GATC_y
Cuts at: 1 1400 2065 2402 2589 3056 3079 3414 3660
Size: 1399 665 337 187 467 23 335 246
Cuts at: 3660 4672 4758 4770 5116 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1399 1012 665 467 449 346 337 335
246 187 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1187 1187
Size: 5564
BtgI C'CryG_G
Cuts at: 623 623
Size: 5564
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3514 3695 3858 3514
Size: 181 163 5220
Fragments arranged by size:
5220 181 163
BtsI GCAGTG_nn'
Cuts at: 123 480 2259 2807 123
Size: 357 1779 548 2880
Fragments arranged by size:
2880 1779 548 357
DraI TTT'AAA
Cuts at: 2563 2563
Size: 5564
DraIII CAC_nnn'GTG
Cuts at: 2133 2133
Size: 5564
DrdI GACnn_nn'nnGTC
Cuts at: 208 3270 4128 208
Size: 3062 858 1644
Fragments arranged by size:
3062 1644 858
EaeI y'GGCC_r
Cuts at: 164 342 424 1034 1473 3149 3323 3714 3741
Size: 178 82 610 439 1676 174 391 27
Cuts at: 3741 5086 164
Size: 1345 642
Fragments arranged by size:
1676 1345 642 610 439 391 178 174
82 27
EagI C'GGCC_G
Cuts at: 342 3149 342
Size: 2807 2757
EarI CTCTTCn'nnn_
Cuts at: 1795 2474 3587 3797 1795
Size: 679 1113 210 3562
Fragments arranged by size:
3562 1113 679 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3772 4082 4228 5374 3772
Size: 310 146 1146 3962
Fragments arranged by size:
3962 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1577 1825 2576 1577
Size: 248 751 4565
Fragments arranged by size:
4565 751 248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 736 1021 1529 2446 3389 3821 4568 4836
Size: 217 285 508 917 943 432 747 268
Cuts at: 4836 519
Size: 1247
Fragments arranged by size:
1247 943 917 747 508 432 285 268
217
EcoO109I rG'GnC_Cy
Cuts at: 727 802 1589 1613 1628 1629 2538 2703 727
Size: 75 787 24 15 1 909 165 3588
Fragments arranged by size:
3588 909 787 165 75 24 15 1
EcoRI G'AATT_C
Cuts at: 2568 2568
Size: 5564
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1632 2329 3583 1632
Size: 697 1254 3613
Fragments arranged by size:
3613 1254 697
FseI GG_CCGG'CC
Cuts at: 1475 1475
Size: 5564
FspI TGC'GCA
Cuts at: 1387 3345 1387
Size: 1958 3606
HaeII r_GCGC'y
Cuts at: 180 248 715 2998 3246 4268 5247 180
Size: 68 467 2283 248 1022 979 497
Fragments arranged by size:
2283 1022 979 497 467 248 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1385 1417 2026 2058 2503 2535 3406 3436 3438
Size: 32 609 32 445 32 871 30 2
Cuts at: 3438 3468 1385
Size: 30 3481
Fragments arranged by size:
3481 871 609 445 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 789 2603 789
Size: 1814 3750
KasI G'GCGC_C
Cuts at: 176 244 2994 3242 176
Size: 68 2750 248 2498
Fragments arranged by size:
2750 2498 248 68
KpnI G_GTAC'C
Cuts at: 2584 2584
Size: 5564
MluI A'CGCG_T
Cuts at: 4997 4997
Size: 5564
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 662 4235 4419 662
Size: 3573 184 1807
Fragments arranged by size:
3573 1807 184
MscI TGG'CCA
Cuts at: 426 1036 3325 426
Size: 610 2289 2665
Fragments arranged by size:
2665 2289 610
MslI CAynn'nnrTG
Cuts at: 1489 3553 3680 1489
Size: 2064 127 3373
Fragments arranged by size:
3373 2064 127
MspA1I CmG'CkG
Cuts at: 410 419 619 1366 1456 1661 2675 2989 3349
Size: 9 200 747 90 205 1014 314 360
Cuts at: 3349 4362 4607 410
Size: 1013 245 1367
Fragments arranged by size:
1367 1014 1013 747 360 314 245 205
200 90 9
NaeI GCC'GGC
Cuts at: 1473 3745 1473
Size: 2272 3292
NarI GG'CG_CC
Cuts at: 177 245 2995 3243 177
Size: 68 2750 248 2498
Fragments arranged by size:
2750 2498 248 68
NcoI C'CATG_G
Cuts at: 623 623
Size: 5564
NdeI CA'TA_TG
Cuts at: 1953 2385 1953
Size: 432 5132
NgoMIV G'CCGG_C
Cuts at: 1471 3743 1471
Size: 2272 3292
NspI r_CATG'y
Cuts at: 486 1068 1230 1711 2617 3117 4024 486
Size: 582 162 481 906 500 907 2026
Fragments arranged by size:
2026 907 906 582 500 481 162
PciI A'CATG_T
Cuts at: 1064 4020 1064
Size: 2956 2608
PflMI CCAn_nnn'nTGG
Cuts at: 422 2133 422
Size: 1711 3853
PmeI GTTT'AAAC
Cuts at: 2563 2563
Size: 5564
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4703 4735 4703
Size: 32 5532
PpuMI rG'GwC_Cy
Cuts at: 1589 1613 2538 1589
Size: 24 925 4615
Fragments arranged by size:
4615 925 24
PsiI TTA'TAA
Cuts at: 2489 2489
Size: 5564
PspOMI G'GGCC_C
Cuts at: 802 1628 2007 802
Size: 826 379 4359
Fragments arranged by size:
4359 826 379
PspXI vC'TCGA_Gb
Cuts at: 181 181
Size: 5564
PstI C_TGCA'G
Cuts at: 968 1455 2303 2611 3296 968
Size: 487 848 308 685 3236
Fragments arranged by size:
3236 848 685 487 308
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5564
PvuII CAG'CTG
Cuts at: 419 619 1366 1456 1661 2989 3349 419
Size: 200 747 90 205 1328 360 2634
Fragments arranged by size:
2634 1328 747 360 205 200 90
RsrII CG'GwC_CG
Cuts at: 3759 3759
Size: 5564
SacI G_AGCT'C
Cuts at: 1579 1827 2578 1579
Size: 248 751 4565
Fragments arranged by size:
4565 751 248
SalI G'TCGA_C
Cuts at: 2601 2601
Size: 5564
SanDI GG'GwC_CC
Cuts at: 1589 2538 1589
Size: 949 4615
SapI GCTCTTCn'nnn_
Cuts at: 3587 3797 3587
Size: 210 5354
SbfI CC_TGCA'GG
Cuts at: 2611 2611
Size: 5564
ScaI AGT'ACT
Cuts at: 2446 5081 2446
Size: 2635 2929
SfcI C'TryA_G
Cuts at: 33 267 922 964 1451 2299 2607 3292 4285
Size: 234 655 42 487 848 308 685 993
Cuts at: 4285 4476 33
Size: 191 1121
Fragments arranged by size:
1121 993 848 685 655 487 308 234
191 42
SfoI GGC'GCC
Cuts at: 178 246 2996 3244 178
Size: 68 2750 248 2498
Fragments arranged by size:
2750 2498 248 68
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5564
SmaI CCC'GGG
Cuts at: 380 1889 2586 380
Size: 1509 697 3358
Fragments arranged by size:
3358 1509 697
SmlI C'TyrA_G
Cuts at: 181 508 2719 3634 4126 4388 181
Size: 327 2211 915 492 262 1357
Fragments arranged by size:
2211 1357 915 492 327 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5564
SphI G_CATG'C
Cuts at: 2617 3117 2617
Size: 500 5064
StuI AGG'CCT
Cuts at: 812 1292 1992 812
Size: 480 700 4384
Fragments arranged by size:
4384 700 480
StyI C'CwwG_G
Cuts at: 320 623 1103 2698 3052 320
Size: 303 480 1595 354 2832
Fragments arranged by size:
2832 1595 480 354 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2036 3147 3280 3739 3948 2036
Size: 1111 133 459 209 3652
Fragments arranged by size:
3652 1111 459 209 133
TatI w'GTAC_w
Cuts at: 1499 2444 2460 5079 1499
Size: 945 16 2619 1984
Fragments arranged by size:
2619 1984 945 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1267 1299 2057 2089 5081 5113 1267
Size: 32 758 32 2992 32 1718
Fragments arranged by size:
2992 1718 758 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2023 3361 2023
Size: 1338 4226
XbaI T'CTAG_A
Cuts at: 2595 2595
Size: 5564
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1227 3059 1227
Size: 1832 3732
XhoI C'TCGA_G
Cuts at: 181 181
Size: 5564
XmaI C'CCGG_G
Cuts at: 378 1887 2584 378
Size: 1509 697 3358
Fragments arranged by size:
3358 1509 697
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BaeI
BbsI BfrBI BglI BmtI BsiWI BsmBI BssHII BstZ17I
ClaI CspCI EcoNI EcoRV FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI