Restriction map for
pF1KB4190
(Circular) (Six-base) MAPSORT of: pF1KB4190.seq Check: 7290 from: 1 to: 4205
pF1KB4190 4205 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 634 634
Size: 4205
AccI GT'mk_AC
Cuts at: 1243 1243
Size: 4205
Acc65I G'GTAC_C
Cuts at: 1221 1221
Size: 4205
AclI AA'CG_TT
Cuts at: 4045 4045
Size: 4205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 847 2030 2462 3209 3477 145
Size: 702 1183 432 747 268 873
Fragments arranged by size:
1183 873 747 702 432 268
AfeI AGC'GCT
Cuts at: 384 384
Size: 4205
AflIII A'CryG_T
Cuts at: 1557 2661 3638 1557
Size: 1104 977 2124
Fragments arranged by size:
2124 1104 977
AgeI A'CCGG_T
Cuts at: 2593 2593
Size: 4205
AleI CACnn'nnGTG
Cuts at: 760 760
Size: 4205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 475 475
Size: 4205
AlwNI CAG_nnn'CTG
Cuts at: 351 788 870 1133 1510 3077 351
Size: 437 82 263 377 1567 1479
Fragments arranged by size:
1567 1479 437 377 263 82
ApaI G_GGCC'C
Cuts at: 120 120
Size: 4205
ApaLI G'TGCA_C
Cuts at: 2975 2975
Size: 4205
ApoI r'AATT_y
Cuts at: 1209 3558 3814 1209
Size: 2349 256 1600
Fragments arranged by size:
2349 1600 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4205
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
AvaI C'yCGr_G
Cuts at: 853 1225 853
Size: 372 3833
BamHI G'GATC_C
Cuts at: 137 1230 137
Size: 1093 3112
BanI G'GyrC_C
Cuts at: 406 682 700 791 1221 1635 1883 1918 406
Size: 276 18 91 430 414 248 35 2693
Fragments arranged by size:
2693 430 414 276 248 91 35 18
BanII G_rGCy'C
Cuts at: 120 1163 1219 2249 120
Size: 1043 56 1030 2076
Fragments arranged by size:
2076 1043 1030 56
BbeI G_GCGC'C
Cuts at: 795 1639 1887 795
Size: 844 248 3113
Fragments arranged by size:
3113 844 248
BbsI GAAGACnn'nnnn_
Cuts at: 468 555 699 1153 468
Size: 87 144 454 3520
Fragments arranged by size:
3520 454 144 87
BbvCI CC'TCA_GC
Cuts at: 379 379
Size: 4205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 690 724 690
Size: 34 4171
BciVI GTATCCnnnnn_n'
Cuts at: 2103 2864 4205 2103
Size: 761 1341 2103
Fragments arranged by size:
2103 1341 761
BclI T'GATC_A
Cuts at: 488 1725 488
Size: 1237 2968
BglI GCCn_nnn'nGGC
Cuts at: 935 935
Size: 4205
BglII A'GATC_T
Cuts at: 1720 3757 1720
Size: 2037 2168
BlpI GC'TnA_GC
Cuts at: 1317 1317
Size: 4205
Bme1580I G_kGCm'C
Cuts at: 120 703 900 1009 1026 1830 1923 2979 120
Size: 583 197 109 17 804 93 1056 1346
Fragments arranged by size:
1346 1056 804 583 197 109 93 17
BmrI ACTGGGnnnn_n'
Cuts at: 261 1417 1498 1833 3977 261
Size: 1156 81 335 2144 489
Fragments arranged by size:
2144 1156 489 335 81
BmtI G_CTAG'C
Cuts at: 725 725
Size: 4205
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4205
Bpu10I CC'TnA_GC
Cuts at: 379 379
Size: 4205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 577 1381 2260 2752 3050 496
Size: 81 804 879 492 298 1651
Fragments arranged by size:
1651 879 804 492 298 81
BsaI GGTCTCn'nnnn_
Cuts at: 246 1051 3909 246
Size: 805 2858 542
Fragments arranged by size:
2858 805 542
BsaAI yAC'GTr
Cuts at: 954 1558 954
Size: 604 3601
BsaBI GATnn'nnATC
Cuts at: 6 1719 3756 6
Size: 1713 2037 455
Fragments arranged by size:
2037 1713 455
BsaHI Gr'CG_yC
Cuts at: 792 1636 1884 2586 4082 792
Size: 844 248 702 1496 915
Fragments arranged by size:
1496 915 844 702 248
BsaWI w'CCGG_w
Cuts at: 1152 1394 1915 2593 2867 3014 3425 1152
Size: 242 521 678 274 147 411 1932
Fragments arranged by size:
1932 678 521 411 274 242 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 289 319 289
Size: 30 4175
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1069 1098 1171 1069
Size: 29 73 4103
Fragments arranged by size:
4103 73 29
BseYI C'CCAG_C
Cuts at: 421 696 884 888 921 1098 1133 1162 1630
Size: 275 188 4 33 177 35 29 468
Cuts at: 1630 2388 2965 3461 421
Size: 758 577 496 1165
Fragments arranged by size:
1165 758 577 496 468 275 188 177
35 33 29 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 127
Size: 4205
BsiEI CG_ry'CG
Cuts at: 104 1747 1793 3001 104
Size: 1643 46 1208 1308
Fragments arranged by size:
1643 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 767 1046 1219 1997 2187 2979 767
Size: 279 173 778 190 792 1993
Fragments arranged by size:
1993 792 778 279 190 173
Bsp1286I G_dGCh'C
Cuts at: 120 703 767 900 1009 1026 1046 1163 1219
Size: 583 64 133 109 17 20 117 56
Cuts at: 1219 1830 1923 1997 2187 2249 2979 120
Size: 611 93 74 190 62 730 1346
Fragments arranged by size:
1346 730 611 583 190 133 117 109
93 74 64 62 56 20 17
BspEI T'CCGG_A
Cuts at: 1152 3425 1152
Size: 2273 1932
BspHI T'CATG_A
Cuts at: 3381 3381
Size: 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 299 593 634 1255 1469 1771 2152 299
Size: 294 41 621 214 302 381 2352
Fragments arranged by size:
2352 621 381 302 294 214 41
BsrBI CCG'CTC
Cuts at: 50 548 2497 2551 4035 4200 50
Size: 498 1949 54 1484 165 55
Fragments arranged by size:
1949 1484 498 165 55 54
BsrDI GCAATG_nn'
Cuts at: 220 2117 220
Size: 1897 2308
BsrFI r'CCGG_y
Cuts at: 2203 2384 2593 2203
Size: 181 209 3815
Fragments arranged by size:
3815 209 181
BssSI C'ACGA_G
Cuts at: 2476 2834 2476
Size: 358 3847
BstAPI GCAn_nnn'nTGC
Cuts at: 1582 1582
Size: 4205
BstBI TT'CG_AA
Cuts at: 2566 2566
Size: 4205
BstEII G'GTnAC_C
Cuts at: 255 354 255
Size: 99 4106
BstXI CCAn_nnnn'nTGG
Cuts at: 270 1197 270
Size: 927 3278
BstYI r'GATC_y
Cuts at: 1 137 1230 1697 1720 2055 2301 3313 3399
Size: 136 1093 467 23 335 246 1012 86
Cuts at: 3399 3411 3757 1
Size: 12 346 449
Fragments arranged by size:
1093 1012 467 449 346 335 246 136
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 439 439
Size: 4205
BtgI C'CryG_G
Cuts at: 269 410 1190 269
Size: 141 780 3284
Fragments arranged by size:
3284 780 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2155 2336 2499 2155
Size: 181 163 3861
Fragments arranged by size:
3861 181 163
BtsI GCAGTG_nn'
Cuts at: 900 1448 900
Size: 548 3657
DraI TTT'AAA
Cuts at: 1204 1204
Size: 4205
DraIII CAC_nnn'GTG
Cuts at: 999 999
Size: 4205
DrdI GACnn_nn'nnGTC
Cuts at: 1911 2769 1911
Size: 858 3347
EaeI y'GGCC_r
Cuts at: 389 1010 1037 1790 1964 2355 2382 3727 389
Size: 621 27 753 174 391 27 1345 867
Fragments arranged by size:
1345 867 753 621 391 174 27 27
EagI C'GGCC_G
Cuts at: 1790 1790
Size: 4205
EarI CTCTTCn'nnn_
Cuts at: 835 1177 2228 2438 835
Size: 342 1051 210 2602
Fragments arranged by size:
2602 1051 342 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1101 2413 2723 2869 4015 1101
Size: 1312 310 146 1146 1291
Fragments arranged by size:
1312 1291 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1217 1217
Size: 4205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 847 2030 2462 3209 3477 145
Size: 702 1183 432 747 268 873
Fragments arranged by size:
1183 873 747 702 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1125 1125
Size: 4205
EcoO109I rG'GnC_Cy
Cuts at: 116 117 161 867 1344 116
Size: 1 44 706 477 2977
Fragments arranged by size:
2977 706 477 44 1
EcoRI G'AATT_C
Cuts at: 1209 1209
Size: 4205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 4205
FspI TGC'GCA
Cuts at: 1986 1986
Size: 4205
HaeII r_GCGC'y
Cuts at: 154 386 795 1190 1639 1887 2909 3888 154
Size: 232 409 395 449 248 1022 979 471
Fragments arranged by size:
1022 979 471 449 409 395 248 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 953 985 2047 2077 2079 2109 289
Size: 32 632 32 1062 30 2 30 2385
Fragments arranged by size:
2385 1062 632 32 32 30 30 2
HincII GTy'rAC
Cuts at: 816 1244 816
Size: 428 3777
KasI G'GCGC_C
Cuts at: 791 1635 1883 791
Size: 844 248 3113
Fragments arranged by size:
3113 844 248
KpnI G_GTAC'C
Cuts at: 1225 1225
Size: 4205
MluI A'CGCG_T
Cuts at: 3638 3638
Size: 4205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2876 3060 2876
Size: 184 4021
MscI TGG'CCA
Cuts at: 1966 1966
Size: 4205
MslI CAynn'nnrTG
Cuts at: 268 484 760 1093 1195 2194 2321 268
Size: 216 276 333 102 999 127 2152
Fragments arranged by size:
2152 999 333 276 216 127 102
MspA1I CmG'CkG
Cuts at: 888 987 1316 1630 1990 3003 3248 888
Size: 99 329 314 360 1013 245 1845
Fragments arranged by size:
1845 1013 360 329 314 245 99
NaeI GCC'GGC
Cuts at: 2386 2386
Size: 4205
NarI GG'CG_CC
Cuts at: 792 1636 1884 792
Size: 844 248 3113
Fragments arranged by size:
3113 844 248
NcoI C'CATG_G
Cuts at: 269 1190 269
Size: 921 3284
NgoMIV G'CCGG_C
Cuts at: 2384 2384
Size: 4205
NheI G'CTAG_C
Cuts at: 721 721
Size: 4205
NspI r_CATG'y
Cuts at: 1258 1758 2665 1258
Size: 500 907 2798
Fragments arranged by size:
2798 907 500
PciI A'CATG_T
Cuts at: 2661 2661
Size: 4205
PflMI CCAn_nnn'nTGG
Cuts at: 351 351
Size: 4205
PfoI T'CCnGG_A
Cuts at: 133 133
Size: 4205
PmeI GTTT'AAAC
Cuts at: 1204 1204
Size: 4205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3344 3376 3344
Size: 32 4173
PspOMI G'GGCC_C
Cuts at: 116 116
Size: 4205
PstI C_TGCA'G
Cuts at: 180 968 1252 1937 180
Size: 788 284 685 2448
Fragments arranged by size:
2448 788 685 284
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4205
PvuII CAG'CTG
Cuts at: 888 1630 1990 888
Size: 742 360 3103
Fragments arranged by size:
3103 742 360
RsrII CG'GwC_CG
Cuts at: 2400 2400
Size: 4205
SacI G_AGCT'C
Cuts at: 1219 1219
Size: 4205
SalI G'TCGA_C
Cuts at: 1242 1242
Size: 4205
SapI GCTCTTCn'nnn_
Cuts at: 1177 2228 2438 1177
Size: 1051 210 2944
Fragments arranged by size:
2944 1051 210
SbfI CC_TGCA'GG
Cuts at: 1252 1252
Size: 4205
ScaI AGT'ACT
Cuts at: 3722 3722
Size: 4205
SfcI C'TryA_G
Cuts at: 33 176 964 1248 1933 2926 3117 33
Size: 143 788 284 685 993 191 1121
Fragments arranged by size:
1121 993 788 685 284 191 143
SfoI GGC'GCC
Cuts at: 793 1637 1885 793
Size: 844 248 3113
Fragments arranged by size:
3113 844 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
SmaI CCC'GGG
Cuts at: 1227 1227
Size: 4205
SmlI C'TyrA_G
Cuts at: 511 592 1360 2275 2767 3029 511
Size: 81 768 915 492 262 1687
Fragments arranged by size:
1687 915 768 492 262 81
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4205
SphI G_CATG'C
Cuts at: 1258 1758 1258
Size: 500 3705
StuI AGG'CCT
Cuts at: 377 1018 377
Size: 641 3564
StyI C'CwwG_G
Cuts at: 269 1013 1190 1339 1693 269
Size: 744 177 149 354 2781
Fragments arranged by size:
2781 744 354 177 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1788 1921 2380 2589 1788
Size: 133 459 209 3404
Fragments arranged by size:
3404 459 209 133
TatI w'GTAC_w
Cuts at: 3720 3720
Size: 4205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3722 3754 3722
Size: 32 4173
Tth111I GACn'n_nGTC
Cuts at: 2002 2002
Size: 4205
XbaI T'CTAG_A
Cuts at: 1236 1236
Size: 4205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 429 1700 429
Size: 1271 2934
XmaI C'CCGG_G
Cuts at: 1225 1225
Size: 4205
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PciI PflMI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BfrBI
BmgBI BpmI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI