Restriction map for
pF1KB4185
(Circular) (Six-base) MAPSORT of: pF1KB4185.seq Check: 604 from: 1 to: 4268
pF1KB4185 4268 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 397 397
Size: 4268
AccI GT'mk_AC
Cuts at: 197 1306 197
Size: 1109 3159
Acc65I G'GTAC_C
Cuts at: 1284 1284
Size: 4268
AclI AA'CG_TT
Cuts at: 4108 4108
Size: 4268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 2093 2525 2878 3540 352
Size: 1741 432 353 662 1080
Fragments arranged by size:
1741 1080 662 432 353
AflIII A'CryG_T
Cuts at: 1620 3420 3701 1620
Size: 1800 281 2187
Fragments arranged by size:
2187 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 879 1573 3011 879
Size: 694 1438 2136
Fragments arranged by size:
2136 1438 694
ApaLI G'TGCA_C
Cuts at: 3106 3106
Size: 4268
ApoI r'AATT_y
Cuts at: 958 1272 3621 3877 958
Size: 314 2349 256 1349
Fragments arranged by size:
2349 1349 314 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4268
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
AvaI C'yCGr_G
Cuts at: 177 1288 177
Size: 1111 3157
BamHI G'GATC_C
Cuts at: 1293 1293
Size: 4268
BanI G'GyrC_C
Cuts at: 1284 1698 1946 1981 1284
Size: 414 248 35 3571
Fragments arranged by size:
3571 414 248 35
BanII G_rGCy'C
Cuts at: 1282 2312 1282
Size: 1030 3238
BbeI G_GCGC'C
Cuts at: 1702 1950 1702
Size: 248 4020
BciVI GTATCCnnnnn_n'
Cuts at: 2166 3222 4268 2166
Size: 1056 1046 2166
Fragments arranged by size:
2166 1056 1046
BclI T'GATC_A
Cuts at: 1788 1788
Size: 4268
BfrBI ATG'CAT
Cuts at: 977 977
Size: 4268
BglII A'GATC_T
Cuts at: 1783 3820 1783
Size: 2037 2231
BlpI GC'TnA_GC
Cuts at: 587 1070 1380 587
Size: 483 310 3475
Fragments arranged by size:
3475 483 310
Bme1580I G_kGCm'C
Cuts at: 1893 1986 3110 1893
Size: 93 1124 3051
Fragments arranged by size:
3051 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1480 1561 1896 4040 1480
Size: 81 335 2144 1708
Fragments arranged by size:
2144 1708 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 602 2635 602
Size: 2033 2235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 542 553 538
Size: 4 11 4253
Fragments arranged by size:
4253 11 4
Bpu10I CC'TnA_GC
Cuts at: 216 216
Size: 4268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 2323 3037 3335 1444
Size: 879 714 298 2377
Fragments arranged by size:
2377 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3972 3972
Size: 4268
BsaAI yAC'GTr
Cuts at: 1621 1621
Size: 4268
BsaBI GATnn'nnATC
Cuts at: 6 1782 3819 6
Size: 1776 2037 455
Fragments arranged by size:
2037 1776 455
BsaHI Gr'CG_yC
Cuts at: 365 394 1699 1947 2649 4145 365
Size: 29 1305 248 702 1496 488
Fragments arranged by size:
1496 1305 702 488 248 29
BsaWI w'CCGG_w
Cuts at: 288 813 941 1031 1457 1978 2656 3067 3214
Size: 525 128 90 426 521 678 411 147
Cuts at: 3214 3488 288
Size: 274 1068
Fragments arranged by size:
1068 678 525 521 426 411 274 147
128 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 646 676 646
Size: 30 4238
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 457 457
Size: 4268
BseYI C'CCAG_C
Cuts at: 1693 2451 3116 3524 1693
Size: 758 665 408 2437
Fragments arranged by size:
2437 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 336 391 336
Size: 55 4213
BsiEI CG_ry'CG
Cuts at: 104 171 1810 1856 3086 104
Size: 67 1639 46 1230 1286
Fragments arranged by size:
1639 1286 1230 67 46
BsiHKAI G_wGCw'C
Cuts at: 1282 2060 2250 3110 1282
Size: 778 190 860 2440
Fragments arranged by size:
2440 860 778 190
BsmI GAATG_Cn'
Cuts at: 1041 1041
Size: 4268
Bsp1286I G_dGCh'C
Cuts at: 1282 1893 1986 2060 2250 2312 3110 1282
Size: 611 93 74 190 62 798 2440
Fragments arranged by size:
2440 798 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1031 1031
Size: 4268
BspHI T'CATG_A
Cuts at: 689 2700 689
Size: 2011 2257
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1318 1532 1834 2215 1318
Size: 214 302 381 3371
Fragments arranged by size:
3371 381 302 214
BsrBI CCG'CTC
Cuts at: 50 381 564 2560 2614 4098 4263 50
Size: 331 183 1996 54 1484 165 55
Fragments arranged by size:
1996 1484 331 183 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1046 1218 2180 1046
Size: 172 962 3134
Fragments arranged by size:
3134 962 172
BsrFI r'CCGG_y
Cuts at: 489 2266 2447 489
Size: 1777 181 2310
Fragments arranged by size:
2310 1777 181
BsrGI T'GTAC_A
Cuts at: 1244 1244
Size: 4268
BssSI C'ACGA_G
Cuts at: 376 574 2539 3247 376
Size: 198 1965 708 1397
Fragments arranged by size:
1965 1397 708 198
BstAPI GCAn_nnn'nTGC
Cuts at: 1645 1645
Size: 4268
BstBI TT'CG_AA
Cuts at: 2629 2629
Size: 4268
BstYI r'GATC_y
Cuts at: 1 1293 1760 1783 2118 2364 2670 2682 2768
Size: 1292 467 23 335 246 306 12 86
Cuts at: 2768 3820 1
Size: 1052 449
Fragments arranged by size:
1292 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 429 503 542 650 429
Size: 74 39 108 4047
Fragments arranged by size:
4047 108 74 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2218 2399 2562 2218
Size: 181 163 3924
Fragments arranged by size:
3924 181 163
BtsI GCAGTG_nn'
Cuts at: 1511 1511
Size: 4268
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 144 179 144
Size: 35 4233
DraI TTT'AAA
Cuts at: 1267 1267
Size: 4268
DraIII CAC_nnn'GTG
Cuts at: 1009 1009
Size: 4268
DrdI GACnn_nn'nnGTC
Cuts at: 1974 3318 1974
Size: 1344 2924
EaeI y'GGCC_r
Cuts at: 539 647 1853 2027 2418 2445 3790 539
Size: 108 1206 174 391 27 1345 1017
Fragments arranged by size:
1345 1206 1017 391 174 108 27
EagI C'GGCC_G
Cuts at: 1853 1853
Size: 4268
EarI CTCTTCn'nnn_
Cuts at: 691 2291 2501 691
Size: 1600 210 2458
Fragments arranged by size:
2458 1600 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2476 3218 3364 4078 2476
Size: 742 146 714 2666
Fragments arranged by size:
2666 742 714 146
EcoICRI GAG'CTC
Cuts at: 1280 1280
Size: 4268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 538 542 553 2093 2525 2878 3540 352
Size: 186 4 11 1540 432 353 662 1080
Fragments arranged by size:
1540 1080 662 432 353 186 11 4
EcoO109I rG'GnC_Cy
Cuts at: 251 385 623 1407 251
Size: 134 238 784 3112
Fragments arranged by size:
3112 784 238 134
EcoRI G'AATT_C
Cuts at: 1272 1272
Size: 4268
EcoRV GAT'ATC
Cuts at: 147 1105 1260 147
Size: 958 155 3155
Fragments arranged by size:
3155 958 155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 4268
FspI TGC'GCA
Cuts at: 2049 2049
Size: 4268
HaeII r_GCGC'y
Cuts at: 481 1702 1950 3180 3951 481
Size: 1221 248 1230 771 798
Fragments arranged by size:
1230 1221 798 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 160 192 199 231 2110 2140 2142 2172 160
Size: 32 7 32 1879 30 2 30 2256
Fragments arranged by size:
2256 1879 32 32 30 30 7 2
HincII GTy'rAC
Cuts at: 351 1307 351
Size: 956 3312
KasI G'GCGC_C
Cuts at: 1698 1946 1698
Size: 248 4020
KpnI G_GTAC'C
Cuts at: 1288 1288
Size: 4268
MluI A'CGCG_T
Cuts at: 3701 3701
Size: 4268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3027 3211 3027
Size: 184 4084
MscI TGG'CCA
Cuts at: 649 2029 649
Size: 1380 2888
MslI CAynn'nnrTG
Cuts at: 2257 2384 2257
Size: 127 4141
MspA1I CmG'CkG
Cuts at: 505 516 544 673 750 1379 1693 2053 2837
Size: 11 28 129 77 629 314 360 784
Cuts at: 2837 3082 505
Size: 245 1691
Fragments arranged by size:
1691 784 629 360 314 245 129 77
28 11
NaeI GCC'GGC
Cuts at: 491 2449 491
Size: 1958 2310
NarI GG'CG_CC
Cuts at: 1699 1947 1699
Size: 248 4020
NgoMIV G'CCGG_C
Cuts at: 489 2447 489
Size: 1958 2310
NsiI A_TGCA'T
Cuts at: 979 979
Size: 4268
NspI r_CATG'y
Cuts at: 1321 1821 3424 1321
Size: 500 1603 2165
Fragments arranged by size:
2165 1603 500
PciI A'CATG_T
Cuts at: 3420 3420
Size: 4268
PmeI GTTT'AAAC
Cuts at: 1267 1267
Size: 4268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2714 2746 2714
Size: 32 4236
PpuMI rG'GwC_Cy
Cuts at: 251 623 251
Size: 372 3896
PshAI GACnn'nnGTC
Cuts at: 256 256
Size: 4268
PstI C_TGCA'G
Cuts at: 1315 2000 1315
Size: 685 3583
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4268
PvuII CAG'CTG
Cuts at: 516 673 750 1693 2053 516
Size: 157 77 943 360 2731
Fragments arranged by size:
2731 943 360 157 77
RsrII CG'GwC_CG
Cuts at: 2463 2463
Size: 4268
SacI G_AGCT'C
Cuts at: 1282 1282
Size: 4268
SacII CC_GC'GG
Cuts at: 506 545 506
Size: 39 4229
SalI G'TCGA_C
Cuts at: 1305 1305
Size: 4268
SanDI GG'GwC_CC
Cuts at: 623 623
Size: 4268
SapI GCTCTTCn'nnn_
Cuts at: 2291 2501 2291
Size: 210 4058
SbfI CC_TGCA'GG
Cuts at: 1315 1315
Size: 4268
ScaI AGT'ACT
Cuts at: 3785 3785
Size: 4268
SfcI C'TryA_G
Cuts at: 33 1178 1311 1996 2964 3155 33
Size: 1145 133 685 968 191 1146
Fragments arranged by size:
1146 1145 968 685 191 133
SfoI GGC'GCC
Cuts at: 1700 1948 1700
Size: 248 4020
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
SmaI CCC'GGG
Cuts at: 1290 1290
Size: 4268
SmlI C'TyrA_G
Cuts at: 1423 2338 3052 3314 1423
Size: 915 714 262 2377
Fragments arranged by size:
2377 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4268
SphI G_CATG'C
Cuts at: 1321 1821 1321
Size: 500 3768
StyI C'CwwG_G
Cuts at: 1402 1756 1402
Size: 354 3914
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1851 1984 2443 2652 1851
Size: 133 459 209 3467
Fragments arranged by size:
3467 459 209 133
TatI w'GTAC_w
Cuts at: 1244 3783 1244
Size: 2539 1729
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 646 678 3785 3817 646
Size: 32 3107 32 1097
Fragments arranged by size:
3107 1097 32 32
Tth111I GACn'n_nGTC
Cuts at: 2065 2065
Size: 4268
XbaI T'CTAG_A
Cuts at: 1299 1299
Size: 4268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 616 1763 616
Size: 1147 3121
XmaI C'CCGG_G
Cuts at: 1288 1288
Size: 4268
ZraI GAC'GTC
Cuts at: 395 395
Size: 4268
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PciI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI BsiWI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI EcoNI FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PsiI PspOMI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI