Restriction map for
pF1KB4178
(Circular) (Six-base) MAPSORT of: pF1KB4178.seq Check: 9383 from: 1 to: 4148
pF1KB4178 4148 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 1186 1186
Size: 4148
Acc65I G'GTAC_C
Cuts at: 1164 1164
Size: 4148
AclI AA'CG_TT
Cuts at: 1142 3988 1142
Size: 2846 1302
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 1973 2405 3152 3420 593
Size: 1380 432 747 268 1321
Fragments arranged by size:
1380 1321 747 432 268
AflII C'TTAA_G
Cuts at: 814 814
Size: 4148
AflIII A'CryG_T
Cuts at: 212 1500 2604 3581 212
Size: 1288 1104 977 779
Fragments arranged by size:
1288 1104 977 779
AgeI A'CCGG_T
Cuts at: 2536 2536
Size: 4148
AlwNI CAG_nnn'CTG
Cuts at: 1453 3020 1453
Size: 1567 2581
ApaI G_GGCC'C
Cuts at: 293 293
Size: 4148
ApaLI G'TGCA_C
Cuts at: 2918 2918
Size: 4148
ApoI r'AATT_y
Cuts at: 1028 1152 3501 3757 1028
Size: 124 2349 256 1419
Fragments arranged by size:
2349 1419 256 124
AseI AT'TA_AT
Cuts at: 20 20
Size: 4148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
AvaI C'yCGr_G
Cuts at: 1168 1168
Size: 4148
BamHI G'GATC_C
Cuts at: 1173 1173
Size: 4148
BanI G'GyrC_C
Cuts at: 274 1164 1578 1826 1861 274
Size: 890 414 248 35 2561
Fragments arranged by size:
2561 890 414 248 35
BanII G_rGCy'C
Cuts at: 293 326 452 689 1162 2192 293
Size: 33 126 237 473 1030 2249
Fragments arranged by size:
2249 1030 473 237 126 33
BbeI G_GCGC'C
Cuts at: 1582 1830 1582
Size: 248 3900
BbsI GAAGACnn'nnnn_
Cuts at: 131 677 131
Size: 546 3602
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4114
BciVI GTATCCnnnnn_n'
Cuts at: 2046 2807 4148 2046
Size: 761 1341 2046
Fragments arranged by size:
2046 1341 761
BclI T'GATC_A
Cuts at: 1668 1668
Size: 4148
BglII A'GATC_T
Cuts at: 462 577 1663 3700 462
Size: 115 1086 2037 910
Fragments arranged by size:
2037 1086 910 115
BlpI GC'TnA_GC
Cuts at: 1260 1260
Size: 4148
Bme1580I G_kGCm'C
Cuts at: 293 1773 1866 2922 293
Size: 1480 93 1056 1519
Fragments arranged by size:
1519 1480 1056 93
BmgBI CAC'GTC
Cuts at: 213 213
Size: 4148
BmrI ACTGGGnnnn_n'
Cuts at: 1360 1441 1776 3920 1360
Size: 81 335 2144 1588
Fragments arranged by size:
2144 1588 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 288 716 288
Size: 428 3720
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 715 727 1324 2203 2695 2993 673
Size: 42 12 597 879 492 298 1828
Fragments arranged by size:
1828 879 597 492 298 42 12
BsaI GGTCTCn'nnnn_
Cuts at: 3852 3852
Size: 4148
BsaAI yAC'GTr
Cuts at: 1501 1501
Size: 4148
BsaBI GATnn'nnATC
Cuts at: 6 1662 3699 6
Size: 1656 2037 455
Fragments arranged by size:
2037 1656 455
BsaHI Gr'CG_yC
Cuts at: 1579 1827 2529 4025 1579
Size: 248 702 1496 1702
Fragments arranged by size:
1702 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1337 1858 2536 2810 2957 3368 1337
Size: 521 678 274 147 411 2117
Fragments arranged by size:
2117 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 578 943 578
Size: 365 3783
BseYI C'CCAG_C
Cuts at: 1573 2331 2908 3404 1573
Size: 758 577 496 2317
Fragments arranged by size:
2317 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 194 259 1690 1736 2944 104
Size: 90 65 1431 46 1208 1308
Fragments arranged by size:
1431 1308 1208 90 65 46
BsiHKAI G_wGCw'C
Cuts at: 452 689 764 1162 1940 2130 2922 452
Size: 237 75 398 778 190 792 1678
Fragments arranged by size:
1678 792 778 398 237 190 75
Bsp1286I G_dGCh'C
Cuts at: 293 326 452 689 764 1162 1773 1866 1940
Size: 33 126 237 75 398 611 93 74
Cuts at: 1940 2130 2192 2922 293
Size: 190 62 730 1519
Fragments arranged by size:
1519 730 611 398 237 190 126 93
75 74 62 33
BspEI T'CCGG_A
Cuts at: 3368 3368
Size: 4148
BspHI T'CATG_A
Cuts at: 3324 3324
Size: 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 239 1198 1412 1714 2095 239
Size: 959 214 302 381 2292
Fragments arranged by size:
2292 959 381 302 214
BsrBI CCG'CTC
Cuts at: 50 264 2440 2494 3978 4143 50
Size: 214 2176 54 1484 165 55
Fragments arranged by size:
2176 1484 214 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2060 2060
Size: 4148
BsrFI r'CCGG_y
Cuts at: 189 254 271 896 2146 2327 2536 189
Size: 65 17 625 1250 181 209 1801
Fragments arranged by size:
1801 1250 625 209 181 65 17
BssSI C'ACGA_G
Cuts at: 2419 2777 2419
Size: 358 3790
BstAPI GCAn_nnn'nTGC
Cuts at: 1525 1525
Size: 4148
BstBI TT'CG_AA
Cuts at: 2509 2509
Size: 4148
BstYI r'GATC_y
Cuts at: 1 462 577 1173 1640 1663 1998 2244 3256
Size: 461 115 596 467 23 335 246 1012
Cuts at: 3256 3342 3354 3700 1
Size: 86 12 346 449
Fragments arranged by size:
1012 596 467 461 449 346 335 246
115 86 23 12
BtgI C'CryG_G
Cuts at: 356 356
Size: 4148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2098 2279 2442 2098
Size: 181 163 3804
Fragments arranged by size:
3804 181 163
BtsI GCAGTG_nn'
Cuts at: 1391 1391
Size: 4148
DraI TTT'AAA
Cuts at: 1147 1147
Size: 4148
DrdI GACnn_nn'nnGTC
Cuts at: 133 217 1854 2712 133
Size: 84 1637 858 1569
Fragments arranged by size:
1637 1569 858 84
EaeI y'GGCC_r
Cuts at: 191 256 898 1733 1907 2298 2325 3670 191
Size: 65 642 835 174 391 27 1345 669
Fragments arranged by size:
1345 835 669 642 391 174 65 27
EagI C'GGCC_G
Cuts at: 191 256 1733 191
Size: 65 1477 2606
Fragments arranged by size:
2606 1477 65
EarI CTCTTCn'nnn_
Cuts at: 151 308 694 747 789 2171 2381 151
Size: 157 386 53 42 1382 210 1918
Fragments arranged by size:
1918 1382 386 210 157 53 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2356 2666 2812 3958 2356
Size: 310 146 1146 2546
Fragments arranged by size:
2546 1146 310 146
EcoICRI GAG'CTC
Cuts at: 450 687 1160 450
Size: 237 473 3438
Fragments arranged by size:
3438 473 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 288 593 716 1973 2405 3152 3420 288
Size: 305 123 1257 432 747 268 1016
Fragments arranged by size:
1257 1016 747 432 305 268 123
EcoO109I rG'GnC_Cy
Cuts at: 289 290 1287 289
Size: 1 997 3150
Fragments arranged by size:
3150 997 1
EcoRI G'AATT_C
Cuts at: 1152 1152
Size: 4148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 4148
FseI GG_CCGG'CC
Cuts at: 900 900
Size: 4148
FspI TGC'GCA
Cuts at: 1929 1929
Size: 4148
HaeII r_GCGC'y
Cuts at: 1582 1830 2852 3831 1582
Size: 248 1022 979 1899
Fragments arranged by size:
1899 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 362 394 967 999 1990 2020 2022 2052 362
Size: 32 573 32 991 30 2 30 2458
Fragments arranged by size:
2458 991 573 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1125 1187 1125
Size: 62 4086
HindIII A'AGCT_T
Cuts at: 541 691 541
Size: 150 3998
KasI G'GCGC_C
Cuts at: 1578 1826 1578
Size: 248 3900
KpnI G_GTAC'C
Cuts at: 1168 1168
Size: 4148
MluI A'CGCG_T
Cuts at: 3581 3581
Size: 4148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1163 2819 3003 1163
Size: 1656 184 2308
Fragments arranged by size:
2308 1656 184
MscI TGG'CCA
Cuts at: 1909 1909
Size: 4148
MslI CAynn'nnrTG
Cuts at: 2137 2264 2137
Size: 127 4021
MspA1I CmG'CkG
Cuts at: 358 1259 1573 1933 2946 3191 358
Size: 901 314 360 1013 245 1315
Fragments arranged by size:
1315 1013 901 360 314 245
NaeI GCC'GGC
Cuts at: 191 273 898 2329 191
Size: 82 625 1431 2010
Fragments arranged by size:
2010 1431 625 82
NarI GG'CG_CC
Cuts at: 1579 1827 1579
Size: 248 3900
NgoMIV G'CCGG_C
Cuts at: 189 271 896 2327 189
Size: 82 625 1431 2010
Fragments arranged by size:
2010 1431 625 82
NspI r_CATG'y
Cuts at: 1201 1701 2608 1201
Size: 500 907 2741
Fragments arranged by size:
2741 907 500
PciI A'CATG_T
Cuts at: 2604 2604
Size: 4148
PfoI T'CCnGG_A
Cuts at: 119 119
Size: 4148
PmeI GTTT'AAAC
Cuts at: 1147 1147
Size: 4148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 4116
PspOMI G'GGCC_C
Cuts at: 289 289
Size: 4148
PstI C_TGCA'G
Cuts at: 236 894 1195 1880 236
Size: 658 301 685 2504
Fragments arranged by size:
2504 685 658 301
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4148
PvuII CAG'CTG
Cuts at: 1573 1933 1573
Size: 360 3788
RsrII CG'GwC_CG
Cuts at: 2343 2343
Size: 4148
SacI G_AGCT'C
Cuts at: 452 689 1162 452
Size: 237 473 3438
Fragments arranged by size:
3438 473 237
SacII CC_GC'GG
Cuts at: 359 359
Size: 4148
SalI G'TCGA_C
Cuts at: 1185 1185
Size: 4148
SapI GCTCTTCn'nnn_
Cuts at: 308 2171 2381 308
Size: 1863 210 2075
Fragments arranged by size:
2075 1863 210
SbfI CC_TGCA'GG
Cuts at: 1195 1195
Size: 4148
ScaI AGT'ACT
Cuts at: 3665 3665
Size: 4148
SfcI C'TryA_G
Cuts at: 33 232 890 1191 1876 2869 3060 33
Size: 199 658 301 685 993 191 1121
Fragments arranged by size:
1121 993 685 658 301 199 191
SfoI GGC'GCC
Cuts at: 1580 1828 1580
Size: 248 3900
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
SmaI CCC'GGG
Cuts at: 1170 1170
Size: 4148
SmlI C'TyrA_G
Cuts at: 688 694 742 814 1303 2218 2710 2972 688
Size: 6 48 72 489 915 492 262 1864
Fragments arranged by size:
1864 915 492 489 262 72 48 6
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4148
SphI G_CATG'C
Cuts at: 1201 1701 1201
Size: 500 3648
StyI C'CwwG_G
Cuts at: 968 1282 1636 968
Size: 314 354 3480
Fragments arranged by size:
3480 354 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 123 1731 1864 2323 2532 123
Size: 1608 133 459 209 1739
Fragments arranged by size:
1739 1608 459 209 133
TatI w'GTAC_w
Cuts at: 3663 3663
Size: 4148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3665 3697 3665
Size: 32 4116
Tth111I GACn'n_nGTC
Cuts at: 1945 1945
Size: 4148
XbaI T'CTAG_A
Cuts at: 435 1179 435
Size: 744 3404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1643 1643
Size: 4148
XmaI C'CCGG_G
Cuts at: 1168 1168
Size: 4148
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI