Restriction map for
pF1KB4172
(Circular) (Six-base) MAPSORT of: pF1KB4172.seq Check: 9739 from: 1 to: 4190
pF1KB4172 4190 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 581 1228 581
Size: 647 3543
Acc65I G'GTAC_C
Cuts at: 1206 1206
Size: 4190
AclI AA'CG_TT
Cuts at: 4030 4030
Size: 4190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2447 3194 3462 2015
Size: 432 747 268 2743
Fragments arranged by size:
2743 747 432 268
AflIII A'CryG_T
Cuts at: 1542 2646 3623 1542
Size: 1104 977 2109
Fragments arranged by size:
2109 1104 977
AgeI A'CCGG_T
Cuts at: 2578 2578
Size: 4190
AleI CACnn'nnGTG
Cuts at: 1018 1018
Size: 4190
AlwNI CAG_nnn'CTG
Cuts at: 1495 3062 1495
Size: 1567 2623
ApaLI G'TGCA_C
Cuts at: 2960 2960
Size: 4190
ApoI r'AATT_y
Cuts at: 483 1194 3543 3799 483
Size: 711 2349 256 874
Fragments arranged by size:
2349 874 711 256
AseI AT'TA_AT
Cuts at: 20 905 20
Size: 885 3305
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 1210 1210
Size: 4190
BamHI G'GATC_C
Cuts at: 1215 1215
Size: 4190
BanI G'GyrC_C
Cuts at: 685 917 1206 1620 1868 1903 685
Size: 232 289 414 248 35 2972
Fragments arranged by size:
2972 414 289 248 232 35
BanII G_rGCy'C
Cuts at: 1204 2234 1204
Size: 1030 3160
BbeI G_GCGC'C
Cuts at: 1624 1872 1624
Size: 248 3942
BbsI GAAGACnn'nnnn_
Cuts at: 218 218
Size: 4190
BciVI GTATCCnnnnn_n'
Cuts at: 794 988 1117 2088 2849 4190 794
Size: 194 129 971 761 1341 794
Fragments arranged by size:
1341 971 794 761 194 129
BclI T'GATC_A
Cuts at: 1710 1710
Size: 4190
BglI GCCn_nnn'nGGC
Cuts at: 668 668
Size: 4190
BglII A'GATC_T
Cuts at: 1705 3742 1705
Size: 2037 2153
BlpI GC'TnA_GC
Cuts at: 800 1302 800
Size: 502 3688
Bme1580I G_kGCm'C
Cuts at: 920 1815 1908 2964 920
Size: 895 93 1056 2146
Fragments arranged by size:
2146 1056 895 93
BmrI ACTGGGnnnn_n'
Cuts at: 1402 1483 1818 3962 1402
Size: 81 335 2144 1630
Fragments arranged by size:
2144 1630 335 81
BmtI G_CTAG'C
Cuts at: 799 1115 799
Size: 316 3874
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 881 2557 881
Size: 1676 2514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 2245 2737 3035 1366
Size: 879 492 298 2521
Fragments arranged by size:
2521 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1158 3894 1158
Size: 2736 1454
BsaAI yAC'GTr
Cuts at: 1543 1543
Size: 4190
BsaBI GATnn'nnATC
Cuts at: 6 1704 3741 6
Size: 1698 2037 455
Fragments arranged by size:
2037 1698 455
BsaHI Gr'CG_yC
Cuts at: 1621 1869 2571 4067 1621
Size: 248 702 1496 1744
Fragments arranged by size:
1744 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1379 1900 2578 2852 2999 3410 1379
Size: 521 678 274 147 411 2159
Fragments arranged by size:
2159 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 279 309 1127 1157 279
Size: 30 818 30 3312
Fragments arranged by size:
3312 818 30 30
BseYI C'CCAG_C
Cuts at: 755 1615 2373 2950 3446 755
Size: 860 758 577 496 1499
Fragments arranged by size:
1499 860 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1732 1778 2986 104
Size: 1628 46 1208 1308
Fragments arranged by size:
1628 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1204 1982 2172 2964 1204
Size: 778 190 792 2430
Fragments arranged by size:
2430 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 920 1204 1815 1908 1982 2172 2234 2964 920
Size: 284 611 93 74 190 62 730 2146
Fragments arranged by size:
2146 730 611 284 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3410 3410
Size: 4190
BspHI T'CATG_A
Cuts at: 3366 3366
Size: 4190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1140 1240 1454 1756 2137 1140
Size: 100 214 302 381 3193
Fragments arranged by size:
3193 381 302 214 100
BsrBI CCG'CTC
Cuts at: 50 2482 2536 4020 4185 50
Size: 2432 54 1484 165 55
Fragments arranged by size:
2432 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2102 2102
Size: 4190
BsrFI r'CCGG_y
Cuts at: 2188 2369 2578 2188
Size: 181 209 3800
Fragments arranged by size:
3800 209 181
BsrGI T'GTAC_A
Cuts at: 947 947
Size: 4190
BssSI C'ACGA_G
Cuts at: 2461 2819 2461
Size: 358 3832
BstAPI GCAn_nnn'nTGC
Cuts at: 1567 1567
Size: 4190
BstBI TT'CG_AA
Cuts at: 2551 2551
Size: 4190
BstXI CCAn_nnnn'nTGG
Cuts at: 592 592
Size: 4190
BstYI r'GATC_y
Cuts at: 1 1215 1682 1705 2040 2286 3298 3384 3396
Size: 1214 467 23 335 246 1012 86 12
Cuts at: 3396 3742 1
Size: 346 449
Fragments arranged by size:
1214 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 689 1100 107
Size: 582 411 3197
Fragments arranged by size:
3197 582 411
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2140 2321 2484 2140
Size: 181 163 3846
Fragments arranged by size:
3846 181 163
BtsI GCAGTG_nn'
Cuts at: 1433 1433
Size: 4190
DraI TTT'AAA
Cuts at: 1189 1189
Size: 4190
DrdI GACnn_nn'nnGTC
Cuts at: 1896 2754 1896
Size: 858 3332
EaeI y'GGCC_r
Cuts at: 669 1775 1949 2340 2367 3712 669
Size: 1106 174 391 27 1345 1147
Fragments arranged by size:
1345 1147 1106 391 174 27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4190
EarI CTCTTCn'nnn_
Cuts at: 2213 2423 2213
Size: 210 3980
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2398 2708 2854 4000 2398
Size: 310 146 1146 2588
Fragments arranged by size:
2588 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1202 1202
Size: 4190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2447 3194 3462 2015
Size: 432 747 268 2743
Fragments arranged by size:
2743 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 926 926
Size: 4190
EcoO109I rG'GnC_Cy
Cuts at: 1329 1329
Size: 4190
EcoRI G'AATT_C
Cuts at: 1194 1194
Size: 4190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 243 297 482 2209 243
Size: 54 185 1727 2224
Fragments arranged by size:
2224 1727 185 54
FspI TGC'GCA
Cuts at: 1971 1971
Size: 4190
HaeII r_GCGC'y
Cuts at: 1624 1872 2894 3873 1624
Size: 248 1022 979 1941
Fragments arranged by size:
1941 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2032 2062 2064 2094 2032
Size: 30 2 30 4128
Fragments arranged by size:
4128 30 30 2
HincII GTy'rAC
Cuts at: 195 237 582 1229 195
Size: 42 345 647 3156
Fragments arranged by size:
3156 647 345 42
HindIII A'AGCT_T
Cuts at: 313 313
Size: 4190
KasI G'GCGC_C
Cuts at: 1620 1868 1620
Size: 248 3942
KpnI G_GTAC'C
Cuts at: 1210 1210
Size: 4190
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 184 509 803 2861 3045 184
Size: 325 294 2058 184 1329
Fragments arranged by size:
2058 1329 325 294 184
MscI TGG'CCA
Cuts at: 671 1951 671
Size: 1280 2910
MslI CAynn'nnrTG
Cuts at: 1018 2179 2306 1018
Size: 1161 127 2902
Fragments arranged by size:
2902 1161 127
MspA1I CmG'CkG
Cuts at: 497 759 1301 1615 1975 2988 3233 497
Size: 262 542 314 360 1013 245 1454
Fragments arranged by size:
1454 1013 542 360 314 262 245
NaeI GCC'GGC
Cuts at: 2371 2371
Size: 4190
NarI GG'CG_CC
Cuts at: 1621 1869 1621
Size: 248 3942
NcoI C'CATG_G
Cuts at: 107 1100 107
Size: 993 3197
NgoMIV G'CCGG_C
Cuts at: 2369 2369
Size: 4190
NheI G'CTAG_C
Cuts at: 795 1111 795
Size: 316 3874
NspI r_CATG'y
Cuts at: 1243 1743 2650 1243
Size: 500 907 2783
Fragments arranged by size:
2783 907 500
PasI CC'CwG_GG
Cuts at: 913 913
Size: 4190
PciI A'CATG_T
Cuts at: 2646 2646
Size: 4190
PfoI T'CCnGG_A
Cuts at: 786 786
Size: 4190
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 4158
PstI C_TGCA'G
Cuts at: 1151 1237 1922 1151
Size: 86 685 3419
Fragments arranged by size:
3419 685 86
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 759 1615 1975 759
Size: 856 360 2974
Fragments arranged by size:
2974 856 360
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 1204 1204
Size: 4190
SalI G'TCGA_C
Cuts at: 580 1227 580
Size: 647 3543
SapI GCTCTTCn'nnn_
Cuts at: 2213 2423 2213
Size: 210 3980
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4190
ScaI AGT'ACT
Cuts at: 1145 3707 1145
Size: 2562 1628
SexAI A'CCwGG_T
Cuts at: 584 584
Size: 4190
SfcI C'TryA_G
Cuts at: 33 1147 1233 1918 2911 3102 33
Size: 1114 86 685 993 191 1121
Fragments arranged by size:
1121 1114 993 685 191 86
SfiI GGCCn_nnn'nGGCC
Cuts at: 668 668
Size: 4190
SfoI GGC'GCC
Cuts at: 1622 1870 1622
Size: 248 3942
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SmaI CCC'GGG
Cuts at: 1212 1212
Size: 4190
SmlI C'TyrA_G
Cuts at: 1345 2260 2752 3014 1345
Size: 915 492 262 2521
Fragments arranged by size:
2521 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3690
StuI AGG'CCT
Cuts at: 662 662
Size: 4190
StyI C'CwwG_G
Cuts at: 107 377 1100 1324 1678 107
Size: 270 723 224 354 2619
Fragments arranged by size:
2619 723 354 270 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1773 1906 2365 2574 1773
Size: 133 459 209 3389
Fragments arranged by size:
3389 459 209 133
TatI w'GTAC_w
Cuts at: 947 1143 3705 947
Size: 196 2562 1432
Fragments arranged by size:
2562 1432 196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 279 311 3707 3739 279
Size: 32 3396 32 730
Fragments arranged by size:
3396 730 32 32
Tth111I GACn'n_nGTC
Cuts at: 586 1987 586
Size: 1401 2789
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1685 1685
Size: 4190
XmaI C'CCGG_G
Cuts at: 1210 1210
Size: 4190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BpmI
Bpu10I BseRI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NotI NruI NsiI PacI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI