Restriction map for
pF1KB4156
(Circular) (Six-base) MAPSORT of: pF1KB4156.seq Check: 842 from: 1 to: 3539
pF1KB4156 3539 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 577 577
Size: 3539
Acc65I G'GTAC_C
Cuts at: 555 555
Size: 3539
AclI AA'CG_TT
Cuts at: 3379 3379
Size: 3539
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1364 1796 2543 2811 1364
Size: 432 747 268 2092
Fragments arranged by size:
2092 747 432 268
AflIII A'CryG_T
Cuts at: 891 1995 2972 891
Size: 1104 977 1458
Fragments arranged by size:
1458 1104 977
AgeI A'CCGG_T
Cuts at: 1927 1927
Size: 3539
AlwNI CAG_nnn'CTG
Cuts at: 346 844 2411 346
Size: 498 1567 1474
Fragments arranged by size:
1567 1474 498
ApaLI G'TGCA_C
Cuts at: 2309 2309
Size: 3539
ApoI r'AATT_y
Cuts at: 543 2892 3148 543
Size: 2349 256 934
Fragments arranged by size:
2349 934 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3539
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3539
AvaI C'yCGr_G
Cuts at: 559 559
Size: 3539
BamHI G'GATC_C
Cuts at: 564 564
Size: 3539
BanI G'GyrC_C
Cuts at: 555 969 1217 1252 555
Size: 414 248 35 2842
Fragments arranged by size:
2842 414 248 35
BanII G_rGCy'C
Cuts at: 553 1583 553
Size: 1030 2509
BbeI G_GCGC'C
Cuts at: 973 1221 973
Size: 248 3291
BciVI GTATCCnnnnn_n'
Cuts at: 488 1437 2198 3539 488
Size: 949 761 1341 488
Fragments arranged by size:
1341 949 761 488
BclI T'GATC_A
Cuts at: 372 1059 372
Size: 687 2852
BglII A'GATC_T
Cuts at: 1054 3091 1054
Size: 2037 1502
BlpI GC'TnA_GC
Cuts at: 651 651
Size: 3539
Bme1580I G_kGCm'C
Cuts at: 1164 1257 2313 1164
Size: 93 1056 2390
Fragments arranged by size:
2390 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 751 832 1167 3311 751
Size: 81 335 2144 979
Fragments arranged by size:
2144 979 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1906 1906
Size: 3539
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 715 1594 2086 2384 436
Size: 279 879 492 298 1591
Fragments arranged by size:
1591 879 492 298 279
BsaI GGTCTCn'nnnn_
Cuts at: 3243 3243
Size: 3539
BsaAI yAC'GTr
Cuts at: 212 892 212
Size: 680 2859
BsaBI GATnn'nnATC
Cuts at: 6 1053 3090 6
Size: 1047 2037 455
Fragments arranged by size:
2037 1047 455
BsaHI Gr'CG_yC
Cuts at: 970 1218 1920 3416 970
Size: 248 702 1496 1093
Fragments arranged by size:
1496 1093 702 248
BsaWI w'CCGG_w
Cuts at: 728 1249 1927 2201 2348 2759 728
Size: 521 678 274 147 411 1508
Fragments arranged by size:
1508 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 201 231 201
Size: 30 3509
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 209 209
Size: 3539
BseYI C'CCAG_C
Cuts at: 964 1722 2299 2795 964
Size: 758 577 496 1708
Fragments arranged by size:
1708 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1081 1127 2335 104
Size: 977 46 1208 1308
Fragments arranged by size:
1308 1208 977 46
BsiHKAI G_wGCw'C
Cuts at: 553 1331 1521 2313 553
Size: 778 190 792 1779
Fragments arranged by size:
1779 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 553 1164 1257 1331 1521 1583 2313 553
Size: 611 93 74 190 62 730 1779
Fragments arranged by size:
1779 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2759 2759
Size: 3539
BspHI T'CATG_A
Cuts at: 2715 2715
Size: 3539
BspMI ACCTGCnnnn'nnnn_
Cuts at: 589 803 1105 1486 589
Size: 214 302 381 2642
Fragments arranged by size:
2642 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1831 1885 3369 3534 50
Size: 1781 54 1484 165 55
Fragments arranged by size:
1781 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1451 1451
Size: 3539
BsrFI r'CCGG_y
Cuts at: 1537 1718 1927 1537
Size: 181 209 3149
Fragments arranged by size:
3149 209 181
BssSI C'ACGA_G
Cuts at: 1810 2168 1810
Size: 358 3181
BstAPI GCAn_nnn'nTGC
Cuts at: 916 916
Size: 3539
BstBI TT'CG_AA
Cuts at: 1900 1900
Size: 3539
BstYI r'GATC_y
Cuts at: 1 342 564 1031 1054 1389 1635 2647 2733
Size: 341 222 467 23 335 246 1012 86
Cuts at: 2733 2745 3091 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 341 335 246 222
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3539
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1489 1670 1833 1489
Size: 181 163 3195
Fragments arranged by size:
3195 181 163
BtsI GCAGTG_nn'
Cuts at: 782 782
Size: 3539
DraI TTT'AAA
Cuts at: 538 538
Size: 3539
DrdI GACnn_nn'nnGTC
Cuts at: 1245 2103 1245
Size: 858 2681
EaeI y'GGCC_r
Cuts at: 337 1124 1298 1689 1716 3061 337
Size: 787 174 391 27 1345 815
Fragments arranged by size:
1345 815 787 391 174 27
EagI C'GGCC_G
Cuts at: 1124 1124
Size: 3539
EarI CTCTTCn'nnn_
Cuts at: 1562 1772 1562
Size: 210 3329
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1747 2057 2203 3349 1747
Size: 310 146 1146 1937
Fragments arranged by size:
1937 1146 310 146
EcoICRI GAG'CTC
Cuts at: 551 551
Size: 3539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1364 1796 2543 2811 1364
Size: 432 747 268 2092
Fragments arranged by size:
2092 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 424 678 424
Size: 254 3285
EcoRI G'AATT_C
Cuts at: 543 543
Size: 3539
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1558 1558
Size: 3539
FspI TGC'GCA
Cuts at: 1320 1320
Size: 3539
HaeII r_GCGC'y
Cuts at: 973 1221 2243 3222 973
Size: 248 1022 979 1290
Fragments arranged by size:
1290 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1381 1411 1413 1443 1381
Size: 30 2 30 3477
Fragments arranged by size:
3477 30 30 2
HincII GTy'rAC
Cuts at: 578 578
Size: 3539
KasI G'GCGC_C
Cuts at: 969 1217 969
Size: 248 3291
KpnI G_GTAC'C
Cuts at: 559 559
Size: 3539
MluI A'CGCG_T
Cuts at: 2972 2972
Size: 3539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2210 2394 2210
Size: 184 3355
MscI TGG'CCA
Cuts at: 1300 1300
Size: 3539
MslI CAynn'nnrTG
Cuts at: 356 1528 1655 356
Size: 1172 127 2240
Fragments arranged by size:
2240 1172 127
MspA1I CmG'CkG
Cuts at: 650 964 1324 2337 2582 650
Size: 314 360 1013 245 1607
Fragments arranged by size:
1607 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1720 1720
Size: 3539
NarI GG'CG_CC
Cuts at: 970 1218 970
Size: 248 3291
NcoI C'CATG_G
Cuts at: 107 107
Size: 3539
NdeI CA'TA_TG
Cuts at: 312 312
Size: 3539
NgoMIV G'CCGG_C
Cuts at: 1718 1718
Size: 3539
NspI r_CATG'y
Cuts at: 592 1092 1999 592
Size: 500 907 2132
Fragments arranged by size:
2132 907 500
PciI A'CATG_T
Cuts at: 1995 1995
Size: 3539
PmeI GTTT'AAAC
Cuts at: 538 538
Size: 3539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2678 2710 2678
Size: 32 3507
PpuMI rG'GwC_Cy
Cuts at: 424 424
Size: 3539
PstI C_TGCA'G
Cuts at: 123 586 1271 123
Size: 463 685 2391
Fragments arranged by size:
2391 685 463
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3539
PvuII CAG'CTG
Cuts at: 964 1324 964
Size: 360 3179
RsrII CG'GwC_CG
Cuts at: 1734 1734
Size: 3539
SacI G_AGCT'C
Cuts at: 553 553
Size: 3539
SalI G'TCGA_C
Cuts at: 576 576
Size: 3539
SapI GCTCTTCn'nnn_
Cuts at: 1562 1772 1562
Size: 210 3329
SbfI CC_TGCA'GG
Cuts at: 586 586
Size: 3539
ScaI AGT'ACT
Cuts at: 3056 3056
Size: 3539
SfcI C'TryA_G
Cuts at: 33 113 119 582 1267 2260 2451 33
Size: 80 6 463 685 993 191 1121
Fragments arranged by size:
1121 993 685 463 191 80 6
SfoI GGC'GCC
Cuts at: 971 1219 971
Size: 248 3291
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3539
SmaI CCC'GGG
Cuts at: 561 561
Size: 3539
SmlI C'TyrA_G
Cuts at: 415 694 1609 2101 2363 415
Size: 279 915 492 262 1591
Fragments arranged by size:
1591 915 492 279 262
SnaBI TAC'GTA
Cuts at: 212 212
Size: 3539
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3539
SphI G_CATG'C
Cuts at: 592 1092 592
Size: 500 3039
StyI C'CwwG_G
Cuts at: 107 673 1027 107
Size: 566 354 2619
Fragments arranged by size:
2619 566 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 120 1122 1255 1714 1923 120
Size: 1002 133 459 209 1736
Fragments arranged by size:
1736 1002 459 209 133
TatI w'GTAC_w
Cuts at: 3054 3054
Size: 3539
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3056 3088 3056
Size: 32 3507
Tth111I GACn'n_nGTC
Cuts at: 1336 1336
Size: 3539
XbaI T'CTAG_A
Cuts at: 570 570
Size: 3539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1034 1034
Size: 3539
XmaI C'CCGG_G
Cuts at: 559 559
Size: 3539
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI
XhoI XmnI ZraI