Restriction map for
pF1KB4139
(Circular) (Six-base) MAPSORT of: pF1KB4139.seq Check: 7325 from: 1 to: 4421
pF1KB4139 4421 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 776 776
Size: 4421
AccI GT'mk_AC
Cuts at: 1459 1459
Size: 4421
Acc65I G'GTAC_C
Cuts at: 1437 1437
Size: 4421
AclI AA'CG_TT
Cuts at: 4261 4261
Size: 4421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 312 1227 2246 2678 3031 3693 312
Size: 915 1019 432 353 662 1040
Fragments arranged by size:
1040 1019 915 662 432 353
AfeI AGC'GCT
Cuts at: 153 153
Size: 4421
AflIII A'CryG_T
Cuts at: 1773 3573 3854 1773
Size: 1800 281 2340
Fragments arranged by size:
2340 1800 281
AleI CACnn'nnGTG
Cuts at: 614 1118 614
Size: 504 3917
AlwNI CAG_nnn'CTG
Cuts at: 1726 3164 1726
Size: 1438 2983
ApaLI G'TGCA_C
Cuts at: 3259 3259
Size: 4421
ApoI r'AATT_y
Cuts at: 115 277 521 605 857 941 1109 1193 1277
Size: 162 244 84 252 84 168 84 84
Cuts at: 1277 1425 3774 4030 115
Size: 148 2349 256 506
Fragments arranged by size:
2349 506 256 252 244 168 162 148
84 84 84 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 4421
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
AvaI C'yCGr_G
Cuts at: 1441 1441
Size: 4421
BamHI G'GATC_C
Cuts at: 1446 1446
Size: 4421
BanI G'GyrC_C
Cuts at: 1437 1851 2099 2134 1437
Size: 414 248 35 3724
Fragments arranged by size:
3724 414 248 35
BanII G_rGCy'C
Cuts at: 1435 2465 1435
Size: 1030 3391
BbeI G_GCGC'C
Cuts at: 1855 2103 1855
Size: 248 4173
BbsI GAAGACnn'nnnn_
Cuts at: 1075 1075
Size: 4421
BciVI GTATCCnnnnn_n'
Cuts at: 2319 3375 4421 2319
Size: 1056 1046 2319
Fragments arranged by size:
2319 1056 1046
BclI T'GATC_A
Cuts at: 1941 1941
Size: 4421
BfrBI ATG'CAT
Cuts at: 1139 1139
Size: 4421
BglII A'GATC_T
Cuts at: 1936 3973 1936
Size: 2037 2384
BlpI GC'TnA_GC
Cuts at: 1533 1533
Size: 4421
Bme1580I G_kGCm'C
Cuts at: 2046 2139 3263 2046
Size: 93 1124 3204
Fragments arranged by size:
3204 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1633 1714 2049 4193 1633
Size: 81 335 2144 1861
Fragments arranged by size:
2144 1861 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 315 2788 315
Size: 2473 1948
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 803 803
Size: 4421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 903 1597 2476 3190 3488 257
Size: 646 694 879 714 298 1190
Fragments arranged by size:
1190 879 714 694 646 298
BsaI GGTCTCn'nnnn_
Cuts at: 4125 4125
Size: 4421
BsaAI yAC'GTr
Cuts at: 1774 1774
Size: 4421
BsaBI GATnn'nnATC
Cuts at: 6 1935 3972 6
Size: 1929 2037 455
Fragments arranged by size:
2037 1929 455
BsaHI Gr'CG_yC
Cuts at: 1852 2100 2802 4298 1852
Size: 248 702 1496 1975
Fragments arranged by size:
1975 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1610 2131 2809 3220 3367 3641 1610
Size: 521 678 411 147 274 2390
Fragments arranged by size:
2390 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 1378 214
Size: 1164 3257
BseYI C'CCAG_C
Cuts at: 1846 2604 3269 3677 1846
Size: 758 665 408 2590
Fragments arranged by size:
2590 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1963 2009 3239 104
Size: 1859 46 1230 1286
Fragments arranged by size:
1859 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1435 2213 2403 3263 1435
Size: 778 190 860 2593
Fragments arranged by size:
2593 860 778 190
BsmI GAATG_Cn'
Cuts at: 400 1141 400
Size: 741 3680
Bsp1286I G_dGCh'C
Cuts at: 1435 2046 2139 2213 2403 2465 3263 1435
Size: 611 93 74 190 62 798 2593
Fragments arranged by size:
2593 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 972 1131 2853 972
Size: 159 1722 2540
Fragments arranged by size:
2540 1722 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 776 1471 1685 1987 2368 776
Size: 695 214 302 381 2829
Fragments arranged by size:
2829 695 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2713 2767 4251 4416 50
Size: 2663 54 1484 165 55
Fragments arranged by size:
2663 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 468 495 730 2333 468
Size: 27 235 1603 2556
Fragments arranged by size:
2556 1603 235 27
BsrFI r'CCGG_y
Cuts at: 2419 2600 2419
Size: 181 4240
BssSI C'ACGA_G
Cuts at: 2692 3400 2692
Size: 708 3713
BstAPI GCAn_nnn'nTGC
Cuts at: 1798 1798
Size: 4421
BstBI TT'CG_AA
Cuts at: 2782 2782
Size: 4421
BstYI r'GATC_y
Cuts at: 1 1446 1913 1936 2271 2517 2823 2835 2921
Size: 1445 467 23 335 246 306 12 86
Cuts at: 2921 3973 1
Size: 1052 449
Fragments arranged by size:
1445 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4421
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 814 2371 2552 2715 814
Size: 1557 181 163 2520
Fragments arranged by size:
2520 1557 181 163
BtsI GCAGTG_nn'
Cuts at: 1664 1664
Size: 4421
DraI TTT'AAA
Cuts at: 120 429 1420 120
Size: 309 991 3121
Fragments arranged by size:
3121 991 309
DrdI GACnn_nn'nnGTC
Cuts at: 2127 3471 2127
Size: 1344 3077
EaeI y'GGCC_r
Cuts at: 2006 2180 2571 2598 3943 2006
Size: 174 391 27 1345 2484
Fragments arranged by size:
2484 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2006 2006
Size: 4421
EarI CTCTTCn'nnn_
Cuts at: 1358 2444 2654 1358
Size: 1086 210 3125
Fragments arranged by size:
3125 1086 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2629 3371 3517 4231 2629
Size: 742 146 714 2819
Fragments arranged by size:
2819 742 714 146
EcoICRI GAG'CTC
Cuts at: 1433 1433
Size: 4421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 312 803 1227 2246 2678 3031 3693 312
Size: 491 424 1019 432 353 662 1040
Fragments arranged by size:
1040 1019 662 491 432 424 353
EcoO109I rG'GnC_Cy
Cuts at: 1560 1560
Size: 4421
EcoRI G'AATT_C
Cuts at: 521 605 857 1109 1193 1277 1425 521
Size: 84 252 252 84 84 148 3517
Fragments arranged by size:
3517 252 252 148 84 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 4421
FspI TGC'GCA
Cuts at: 2202 2202
Size: 4421
HaeII r_GCGC'y
Cuts at: 155 1855 2103 3333 4104 155
Size: 1700 248 1230 771 472
Fragments arranged by size:
1700 1230 771 472 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 281 313 2263 2293 2295 2325 281
Size: 32 1950 30 2 30 2377
Fragments arranged by size:
2377 1950 32 30 30 2
HincII GTy'rAC
Cuts at: 1460 1460
Size: 4421
HindIII A'AGCT_T
Cuts at: 921 1257 921
Size: 336 4085
KasI G'GCGC_C
Cuts at: 1851 2099 1851
Size: 248 4173
KpnI G_GTAC'C
Cuts at: 1441 1441
Size: 4421
MluI A'CGCG_T
Cuts at: 3854 3854
Size: 4421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 460 3180 3364 460
Size: 2720 184 1517
Fragments arranged by size:
2720 1517 184
MscI TGG'CCA
Cuts at: 2182 2182
Size: 4421
MslI CAynn'nnrTG
Cuts at: 530 614 698 866 977 1034 1118 1202 2410
Size: 84 84 168 111 57 84 84 1208
Cuts at: 2410 2537 530
Size: 127 2414
Fragments arranged by size:
2414 1208 168 127 111 84 84 84
84 57
MspA1I CmG'CkG
Cuts at: 1532 1846 2206 2990 3235 1532
Size: 314 360 784 245 2718
Fragments arranged by size:
2718 784 360 314 245
NaeI GCC'GGC
Cuts at: 2602 2602
Size: 4421
NarI GG'CG_CC
Cuts at: 1852 2100 1852
Size: 248 4173
NcoI C'CATG_G
Cuts at: 107 107
Size: 4421
NgoMIV G'CCGG_C
Cuts at: 2600 2600
Size: 4421
NsiI A_TGCA'T
Cuts at: 1141 1141
Size: 4421
NspI r_CATG'y
Cuts at: 243 1474 1974 3577 243
Size: 1231 500 1603 1087
Fragments arranged by size:
1603 1231 1087 500
PciI A'CATG_T
Cuts at: 3573 3573
Size: 4421
PmeI GTTT'AAAC
Cuts at: 1420 1420
Size: 4421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2867 2899 2867
Size: 32 4389
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 578 610 578
Size: 32 4389
PstI C_TGCA'G
Cuts at: 1468 2153 1468
Size: 685 3736
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4421
PvuII CAG'CTG
Cuts at: 1846 2206 1846
Size: 360 4061
RsrII CG'GwC_CG
Cuts at: 2616 2616
Size: 4421
SacI G_AGCT'C
Cuts at: 1435 1435
Size: 4421
SalI G'TCGA_C
Cuts at: 1458 1458
Size: 4421
SapI GCTCTTCn'nnn_
Cuts at: 2444 2654 2444
Size: 210 4211
SbfI CC_TGCA'GG
Cuts at: 1468 1468
Size: 4421
ScaI AGT'ACT
Cuts at: 3938 3938
Size: 4421
SfcI C'TryA_G
Cuts at: 33 1464 2149 3117 3308 33
Size: 1431 685 968 191 1146
Fragments arranged by size:
1431 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1853 2101 1853
Size: 248 4173
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
SmaI CCC'GGG
Cuts at: 1443 1443
Size: 4421
SmlI C'TyrA_G
Cuts at: 272 918 1576 2491 3205 3467 272
Size: 646 658 915 714 262 1226
Fragments arranged by size:
1226 915 714 658 646 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4421
SphI G_CATG'C
Cuts at: 1474 1974 1474
Size: 500 3921
StuI AGG'CCT
Cuts at: 487 568 652 904 1156 1240 487
Size: 81 84 252 252 84 3668
Fragments arranged by size:
3668 252 252 84 84 81
StyI C'CwwG_G
Cuts at: 107 1555 1909 107
Size: 1448 354 2619
Fragments arranged by size:
2619 1448 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2004 2137 2596 2805 2004
Size: 133 459 209 3620
Fragments arranged by size:
3620 459 209 133
TatI w'GTAC_w
Cuts at: 3936 3936
Size: 4421
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3938 3970 3938
Size: 32 4389
Tth111I GACn'n_nGTC
Cuts at: 2218 2218
Size: 4421
XbaI T'CTAG_A
Cuts at: 1452 1452
Size: 4421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1916 1916
Size: 4421
XmaI C'CCGG_G
Cuts at: 1441 1441
Size: 4421
XmnI GAAnn'nnTTC
Cuts at: 366 1240 1281 366
Size: 874 41 3506
Fragments arranged by size:
3506 874 41
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PmeI PpiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI