Restriction map for
pF1KB4124
(Circular) (Six-base) MAPSORT of: pF1KB4124.seq Check: 3297 from: 1 to: 6092
pF1KB4124 6092 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1597 3130 1597
Size: 1533 4559
Acc65I G'GTAC_C
Cuts at: 125 3108 125
Size: 2983 3109
AclI AA'CG_TT
Cuts at: 5932 5932
Size: 6092
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 1586 3917 4349 4702 5364 964
Size: 622 2331 432 353 662 1692
Fragments arranged by size:
2331 1692 662 622 432 353
AflII C'TTAA_G
Cuts at: 1377 1377
Size: 6092
AflIII A'CryG_T
Cuts at: 805 2609 3444 5244 5525 805
Size: 1804 835 1800 281 1372
Fragments arranged by size:
1804 1800 1372 835 281
AleI CACnn'nnGTG
Cuts at: 616 857 616
Size: 241 5851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 875 875
Size: 6092
AlwNI CAG_nnn'CTG
Cuts at: 766 2458 3397 4835 766
Size: 1692 939 1438 2023
Fragments arranged by size:
2023 1692 1438 939
ApaLI G'TGCA_C
Cuts at: 4930 4930
Size: 6092
ApoI r'AATT_y
Cuts at: 415 775 1894 2020 2483 2527 2737 2756 3096
Size: 360 1119 126 463 44 210 19 340
Cuts at: 3096 5445 5701 415
Size: 2349 256 806
Fragments arranged by size:
2349 1119 806 463 360 340 256 210
126 44 19
AseI AT'TA_AT
Cuts at: 20 1613 20
Size: 1593 4499
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6092
AvaI C'yCGr_G
Cuts at: 2432 3112 2432
Size: 680 5412
BamHI G'GATC_C
Cuts at: 3117 3117
Size: 6092
BanI G'GyrC_C
Cuts at: 125 1903 3108 3522 3770 3805 125
Size: 1778 1205 414 248 35 2412
Fragments arranged by size:
2412 1778 1205 414 248 35
BanII G_rGCy'C
Cuts at: 344 1065 2247 3106 4136 344
Size: 721 1182 859 1030 2300
Fragments arranged by size:
2300 1182 1030 859 721
BbeI G_GCGC'C
Cuts at: 3526 3774 3526
Size: 248 5844
BbsI GAAGACnn'nnnn_
Cuts at: 433 956 433
Size: 523 5569
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1418 1452 1418
Size: 34 6058
BciVI GTATCCnnnnn_n'
Cuts at: 1261 3990 5046 6092 1261
Size: 2729 1056 1046 1261
Fragments arranged by size:
2729 1261 1056 1046
BclI T'GATC_A
Cuts at: 3612 3612
Size: 6092
BfrBI ATG'CAT
Cuts at: 2254 2254
Size: 6092
BglI GCCn_nnn'nGGC
Cuts at: 2681 2681
Size: 6092
BglII A'GATC_T
Cuts at: 975 3607 5644 975
Size: 2632 2037 1423
Fragments arranged by size:
2632 2037 1423
BlpI GC'TnA_GC
Cuts at: 3204 3204
Size: 6092
Bme1580I G_kGCm'C
Cuts at: 884 3717 3810 4934 884
Size: 2833 93 1124 2042
Fragments arranged by size:
2833 2042 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 463 3304 3385 3720 5864 463
Size: 2841 81 335 2144 691
Fragments arranged by size:
2841 2144 691 335 81
BmtI G_CTAG'C
Cuts at: 1327 1327
Size: 6092
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 547 4459 547
Size: 3912 2180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 467 1014 1722 467
Size: 547 708 4837
Fragments arranged by size:
4837 708 547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 956 3268 4147 4861 5159 956
Size: 2312 879 714 298 1889
Fragments arranged by size:
2312 1889 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5796 5796
Size: 6092
BsaAI yAC'GTr
Cuts at: 3445 3445
Size: 6092
BsaBI GATnn'nnATC
Cuts at: 6 3606 5643 6
Size: 3600 2037 455
Fragments arranged by size:
3600 2037 455
BsaHI Gr'CG_yC
Cuts at: 3523 3771 4473 5969 3523
Size: 248 702 1496 3646
Fragments arranged by size:
3646 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2522 3281 3802 4480 4891 5038 5312 2522
Size: 759 521 678 411 147 274 3302
Fragments arranged by size:
3302 759 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1083 1113 1083
Size: 30 6062
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1017 1017
Size: 6092
BseYI C'CCAG_C
Cuts at: 1277 1783 2676 3517 4275 4940 5348 1277
Size: 506 893 841 758 665 408 2021
Fragments arranged by size:
2021 893 841 758 665 506 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 839 881 1091 1242 2033 839
Size: 42 210 151 791 4898
Fragments arranged by size:
4898 791 210 151 42
BsiEI CG_ry'CG
Cuts at: 104 3634 3680 4910 104
Size: 3530 46 1230 1286
Fragments arranged by size:
3530 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 988 3051 3106 3884 4074 4934 988
Size: 2063 55 778 190 860 2146
Fragments arranged by size:
2146 2063 860 778 190 55
BsmI GAATG_Cn'
Cuts at: 2309 3032 3077 2309
Size: 723 45 5324
Fragments arranged by size:
5324 723 45
Bsp1286I G_dGCh'C
Cuts at: 344 884 988 1065 2247 3051 3106 3717 3810
Size: 540 104 77 1182 804 55 611 93
Cuts at: 3810 3884 4074 4136 4934 344
Size: 74 190 62 798 1502
Fragments arranged by size:
1502 1182 804 798 611 540 190 104
93 77 74 62 55
BspHI T'CATG_A
Cuts at: 2213 3023 4524 2213
Size: 810 1501 3781
Fragments arranged by size:
3781 1501 810
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1328 3142 3356 3658 4039 1328
Size: 1814 214 302 381 3381
Fragments arranged by size:
3381 1814 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4384 4438 5922 6087 50
Size: 4334 54 1484 165 55
Fragments arranged by size:
4334 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 393 551 1763 4004 393
Size: 158 1212 2241 2481
Fragments arranged by size:
2481 2241 1212 158
BsrFI r'CCGG_y
Cuts at: 4090 4271 4090
Size: 181 5911
BssSI C'ACGA_G
Cuts at: 4363 5071 4363
Size: 708 5384
BstAPI GCAn_nnn'nTGC
Cuts at: 3469 3469
Size: 6092
BstBI TT'CG_AA
Cuts at: 2775 4453 2775
Size: 1678 4414
BstEII G'GTnAC_C
Cuts at: 580 580
Size: 6092
BstXI CCAn_nnnn'nTGG
Cuts at: 1285 1797 2495 1285
Size: 512 698 4882
Fragments arranged by size:
4882 698 512
BstYI r'GATC_y
Cuts at: 1 975 3117 3584 3607 3942 4188 4494 4506
Size: 974 2142 467 23 335 246 306 12
Cuts at: 4506 4592 5644 1
Size: 86 1052 449
Fragments arranged by size:
2142 1052 974 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 424 424
Size: 6092
BtgI C'CryG_G
Cuts at: 107 948 2666 107
Size: 841 1718 3533
Fragments arranged by size:
3533 1718 841
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1883 4042 4223 4386 1883
Size: 2159 181 163 3589
Fragments arranged by size:
3589 2159 181 163
BtsI GCAGTG_nn'
Cuts at: 822 852 864 2043 3335 822
Size: 30 12 1179 1292 3579
Fragments arranged by size:
3579 1292 1179 30 12
DraI TTT'AAA
Cuts at: 1893 3091 1893
Size: 1198 4894
DrdI GACnn_nn'nnGTC
Cuts at: 3798 5142 3798
Size: 1344 4748
EaeI y'GGCC_r
Cuts at: 945 2040 3677 3851 4242 4269 5614 945
Size: 1095 1637 174 391 27 1345 1423
Fragments arranged by size:
1637 1423 1345 1095 391 174 27
EagI C'GGCC_G
Cuts at: 3677 3677
Size: 6092
EarI CTCTTCn'nnn_
Cuts at: 1868 2837 4115 4325 1868
Size: 969 1278 210 3635
Fragments arranged by size:
3635 1278 969 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4300 5042 5188 5902 4300
Size: 742 146 714 4490
Fragments arranged by size:
4490 742 714 146
EcoICRI GAG'CTC
Cuts at: 3104 3104
Size: 6092
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 467 964 1014 1586 1722 3917 4349 4702 5364
Size: 497 50 572 136 2195 432 353 662
Cuts at: 5364 467
Size: 1195
Fragments arranged by size:
2195 1195 662 572 497 432 353 136
50
EcoO109I rG'GnC_Cy
Cuts at: 1938 2364 3231 1938
Size: 426 867 4799
Fragments arranged by size:
4799 867 426
EcoRI G'AATT_C
Cuts at: 2020 2756 3096 2020
Size: 736 340 5016
Fragments arranged by size:
5016 736 340
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 601 4111 601
Size: 3510 2582
FspI TGC'GCA
Cuts at: 3873 3873
Size: 6092
HaeII r_GCGC'y
Cuts at: 3526 3774 5004 5775 3526
Size: 248 1230 771 3843
Fragments arranged by size:
3843 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 240 272 3934 3964 3966 3996 240
Size: 32 3662 30 2 30 2336
Fragments arranged by size:
3662 2336 32 30 30 2
HincII GTy'rAC
Cuts at: 2391 3131 2391
Size: 740 5352
HindIII A'AGCT_T
Cuts at: 604 604
Size: 6092
KasI G'GCGC_C
Cuts at: 3522 3770 3522
Size: 248 5844
KpnI G_GTAC'C
Cuts at: 129 3112 129
Size: 2983 3109
MluI A'CGCG_T
Cuts at: 5525 5525
Size: 6092
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 221 1021 2681 2733 2856 4851 5035 221
Size: 800 1660 52 123 1995 184 1278
Fragments arranged by size:
1995 1660 1278 800 184 123 52
MscI TGG'CCA
Cuts at: 3853 3853
Size: 6092
MslI CAynn'nnrTG
Cuts at: 616 857 1312 1514 1768 1798 2168 3065 3082
Size: 241 455 202 254 30 370 897 17
Cuts at: 3082 4081 4208 616
Size: 999 127 2500
Fragments arranged by size:
2500 999 897 455 370 254 241 202
127 30 17
MspA1I CmG'CkG
Cuts at: 1476 1732 2680 2932 3203 3517 3877 4661 4906
Size: 256 948 252 271 314 360 784 245
Cuts at: 4906 1476
Size: 2662
Fragments arranged by size:
2662 948 784 360 314 271 256 252
245
NaeI GCC'GGC
Cuts at: 4273 4273
Size: 6092
NarI GG'CG_CC
Cuts at: 3523 3771 3523
Size: 248 5844
NcoI C'CATG_G
Cuts at: 107 2666 107
Size: 2559 3533
NgoMIV G'CCGG_C
Cuts at: 4271 4271
Size: 6092
NheI G'CTAG_C
Cuts at: 1323 1323
Size: 6092
NsiI A_TGCA'T
Cuts at: 2256 2256
Size: 6092
NspI r_CATG'y
Cuts at: 809 1091 1275 2613 3145 3645 5248 809
Size: 282 184 1338 532 500 1603 1653
Fragments arranged by size:
1653 1603 1338 532 500 282 184
PasI CC'CwG_GG
Cuts at: 2802 2802
Size: 6092
PciI A'CATG_T
Cuts at: 805 2609 5244 805
Size: 1804 2635 1653
Fragments arranged by size:
2635 1804 1653
PflMI CCAn_nnn'nTGG
Cuts at: 265 269 336 265
Size: 4 67 6021
Fragments arranged by size:
6021 67 4
PfoI T'CCnGG_A
Cuts at: 444 444
Size: 6092
PmeI GTTT'AAAC
Cuts at: 3091 3091
Size: 6092
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4538 4570 4538
Size: 32 6060
PpuMI rG'GwC_Cy
Cuts at: 1938 2364 1938
Size: 426 5666
PshAI GACnn'nnGTC
Cuts at: 636 808 636
Size: 172 5920
PsiI TTA'TAA
Cuts at: 2629 2629
Size: 6092
PstI C_TGCA'G
Cuts at: 561 859 1173 1475 2038 3139 3824 561
Size: 298 314 302 563 1101 685 2829
Fragments arranged by size:
2829 1101 685 563 314 302 298
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6092
PvuII CAG'CTG
Cuts at: 1732 2680 2932 3517 3877 1732
Size: 948 252 585 360 3947
Fragments arranged by size:
3947 948 585 360 252
RsrII CG'GwC_CG
Cuts at: 4287 4287
Size: 6092
SacI G_AGCT'C
Cuts at: 3106 3106
Size: 6092
SalI G'TCGA_C
Cuts at: 3129 3129
Size: 6092
SapI GCTCTTCn'nnn_
Cuts at: 1868 4115 4325 1868
Size: 2247 210 3635
Fragments arranged by size:
3635 2247 210
SbfI CC_TGCA'GG
Cuts at: 3139 3139
Size: 6092
ScaI AGT'ACT
Cuts at: 206 452 1469 5609 206
Size: 246 1017 4140 689
Fragments arranged by size:
4140 1017 689 246
SexAI A'CCwGG_T
Cuts at: 320 320
Size: 6092
SfcI C'TryA_G
Cuts at: 33 557 855 1169 1200 1471 1721 1727 1778
Size: 524 298 314 31 271 250 6 51
Cuts at: 1778 2034 3135 3820 4788 4979 33
Size: 256 1101 685 968 191 1146
Fragments arranged by size:
1146 1101 968 685 524 314 298 271
256 250 191 51 31 6
SfoI GGC'GCC
Cuts at: 3524 3772 3524
Size: 248 5844
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6092
SmaI CCC'GGG
Cuts at: 3114 3114
Size: 6092
SmlI C'TyrA_G
Cuts at: 971 1377 3247 4162 4876 5138 971
Size: 406 1870 915 714 262 1925
Fragments arranged by size:
1925 1870 915 714 406 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6092
SphI G_CATG'C
Cuts at: 1091 3145 3645 1091
Size: 2054 500 3538
Fragments arranged by size:
3538 2054 500
SspI AAT'ATT
Cuts at: 1208 2382 1208
Size: 1174 4918
StuI AGG'CCT
Cuts at: 1098 1098
Size: 6092
StyI C'CwwG_G
Cuts at: 107 576 585 1967 2666 3226 3580 107
Size: 469 9 1382 699 560 354 2619
Fragments arranged by size:
2619 1382 699 560 469 354 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 257 276 2679 3675 3808 4267 4476 257
Size: 19 2403 996 133 459 209 1873
Fragments arranged by size:
2403 1873 996 459 209 133 19
TatI w'GTAC_w
Cuts at: 204 450 482 707 1467 5607 204
Size: 246 32 225 760 4140 689
Fragments arranged by size:
4140 760 689 246 225 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1083 1115 5609 5641 1083
Size: 32 4494 32 1534
Fragments arranged by size:
4494 1534 32 32
Tth111I GACn'n_nGTC
Cuts at: 3889 3889
Size: 6092
XbaI T'CTAG_A
Cuts at: 2760 3123 2760
Size: 363 5729
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 266 583 1149 3587 266
Size: 317 566 2438 2771
Fragments arranged by size:
2771 2438 566 317
XmaI C'CCGG_G
Cuts at: 3112 3112
Size: 6092
XmnI GAAnn'nnTTC
Cuts at: 1244 1244
Size: 6092
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AloI ApaI AscI
AvrII BaeI BbvCI BmgBI Bpu10I BsiWI BsmBI BspEI
BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NotI NruI PacI
PmlI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI