Restriction map for
pF1KB4120
(Circular) (Six-base) MAPSORT of: pF1KB4120.seq Check: 2353 from: 1 to: 4466
pF1KB4120 4466 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 495 495
Size: 4466
AccI GT'mk_AC
Cuts at: 1504 1504
Size: 4466
Acc65I G'GTAC_C
Cuts at: 1482 1482
Size: 4466
AclI AA'CG_TT
Cuts at: 4306 4306
Size: 4466
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2291 2723 3470 3738 2291
Size: 432 747 268 3019
Fragments arranged by size:
3019 747 432 268
AflIII A'CryG_T
Cuts at: 1818 2922 3899 1818
Size: 1104 977 2385
Fragments arranged by size:
2385 1104 977
AgeI A'CCGG_T
Cuts at: 2854 2854
Size: 4466
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 618 650 618
Size: 32 4434
AlwNI CAG_nnn'CTG
Cuts at: 183 333 943 1771 3338 183
Size: 150 610 828 1567 1311
Fragments arranged by size:
1567 1311 828 610 150
ApaI G_GGCC'C
Cuts at: 1266 1266
Size: 4466
ApaLI G'TGCA_C
Cuts at: 3236 3236
Size: 4466
ApoI r'AATT_y
Cuts at: 1095 1470 3819 4075 1095
Size: 375 2349 256 1486
Fragments arranged by size:
2349 1486 375 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4466
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
AvaI C'yCGr_G
Cuts at: 188 930 1486 188
Size: 742 556 3168
Fragments arranged by size:
3168 742 556
BamHI G'GATC_C
Cuts at: 1251 1491 1251
Size: 240 4226
BanI G'GyrC_C
Cuts at: 1482 1896 2144 2179 1482
Size: 414 248 35 3769
Fragments arranged by size:
3769 414 248 35
BanII G_rGCy'C
Cuts at: 937 1266 1480 2510 937
Size: 329 214 1030 2893
Fragments arranged by size:
2893 1030 329 214
BbeI G_GCGC'C
Cuts at: 1900 2148 1900
Size: 248 4218
BbsI GAAGACnn'nnnn_
Cuts at: 362 362
Size: 4466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 961 995 961
Size: 34 4432
BciVI GTATCCnnnnn_n'
Cuts at: 1012 2364 3125 4466 1012
Size: 1352 761 1341 1012
Fragments arranged by size:
1352 1341 1012 761
BclI T'GATC_A
Cuts at: 1986 1986
Size: 4466
BfrBI ATG'CAT
Cuts at: 307 307
Size: 4466
BglII A'GATC_T
Cuts at: 1981 4018 1981
Size: 2037 2429
BlpI GC'TnA_GC
Cuts at: 936 1058 1578 936
Size: 122 520 3824
Fragments arranged by size:
3824 520 122
Bme1580I G_kGCm'C
Cuts at: 1266 2091 2184 3240 1266
Size: 825 93 1056 2492
Fragments arranged by size:
2492 1056 825 93
BmrI ACTGGGnnnn_n'
Cuts at: 691 945 1678 1759 2094 4238 691
Size: 254 733 81 335 2144 919
Fragments arranged by size:
2144 919 733 335 254 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 4466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 310
Size: 4466
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1642 2521 3013 3311 1642
Size: 879 492 298 2797
Fragments arranged by size:
2797 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 247 804 4170 247
Size: 557 3366 543
Fragments arranged by size:
3366 557 543
BsaAI yAC'GTr
Cuts at: 1819 1819
Size: 4466
BsaBI GATnn'nnATC
Cuts at: 6 1250 1980 4017 6
Size: 1244 730 2037 455
Fragments arranged by size:
2037 1244 730 455
BsaHI Gr'CG_yC
Cuts at: 1897 2145 2847 4343 1897
Size: 248 702 1496 2020
Fragments arranged by size:
2020 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1655 2176 2854 3128 3275 3686 1655
Size: 521 678 274 147 411 2435
Fragments arranged by size:
2435 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 130 160 618 648 130
Size: 30 458 30 3948
Fragments arranged by size:
3948 458 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 151 265 1222 148
Size: 3 114 957 3392
Fragments arranged by size:
3392 957 114 3
BseYI C'CCAG_C
Cuts at: 1891 2649 3226 3722 1891
Size: 758 577 496 2635
Fragments arranged by size:
2635 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2008 2054 3262 104
Size: 1904 46 1208 1308
Fragments arranged by size:
1904 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1480 2258 2448 3240 1480
Size: 778 190 792 2706
Fragments arranged by size:
2706 792 778 190
BsmI GAATG_Cn'
Cuts at: 881 881
Size: 4466
Bsp1286I G_dGCh'C
Cuts at: 937 1266 1480 2091 2184 2258 2448 2510 3240
Size: 329 214 611 93 74 190 62 730
Cuts at: 3240 937
Size: 2163
Fragments arranged by size:
2163 730 611 329 214 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3686 3686
Size: 4466
BspHI T'CATG_A
Cuts at: 3642 3642
Size: 4466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 495 1516 1730 2032 2413 495
Size: 1021 214 302 381 2548
Fragments arranged by size:
2548 1021 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2758 2812 4296 4461 50
Size: 2708 54 1484 165 55
Fragments arranged by size:
2708 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 887 2378 887
Size: 1491 2975
BsrFI r'CCGG_y
Cuts at: 2464 2645 2854 2464
Size: 181 209 4076
Fragments arranged by size:
4076 209 181
BssSI C'ACGA_G
Cuts at: 2737 3095 2737
Size: 358 4108
BstAPI GCAn_nnn'nTGC
Cuts at: 1843 1843
Size: 4466
BstBI TT'CG_AA
Cuts at: 2827 2827
Size: 4466
BstYI r'GATC_y
Cuts at: 1 140 1251 1491 1958 1981 2316 2562 3574
Size: 139 1111 240 467 23 335 246 1012
Cuts at: 3574 3660 3672 4018 1
Size: 86 12 346 449
Fragments arranged by size:
1111 1012 467 449 346 335 246 240
139 86 23 12
BtgI C'CryG_G
Cuts at: 107 558 107
Size: 451 4015
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2416 2597 2760 2416
Size: 181 163 4122
Fragments arranged by size:
4122 181 163
BtsI GCAGTG_nn'
Cuts at: 1709 1709
Size: 4466
ClaI AT'CG_AT
Cuts at: 530 530
Size: 4466
DraI TTT'AAA
Cuts at: 803 1465 803
Size: 662 3804
DraIII CAC_nnn'GTG
Cuts at: 826 826
Size: 4466
DrdI GACnn_nn'nnGTC
Cuts at: 2172 3030 2172
Size: 858 3608
EaeI y'GGCC_r
Cuts at: 311 2051 2225 2616 2643 3988 311
Size: 1740 174 391 27 1345 789
Fragments arranged by size:
1740 1345 789 391 174 27
EagI C'GGCC_G
Cuts at: 2051 2051
Size: 4466
EarI CTCTTCn'nnn_
Cuts at: 420 2489 2699 420
Size: 2069 210 2187
Fragments arranged by size:
2187 2069 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2674 2984 3130 4276 2674
Size: 310 146 1146 2864
Fragments arranged by size:
2864 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1478 1478
Size: 4466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 2291 2723 3470 3738 310
Size: 1981 432 747 268 1038
Fragments arranged by size:
1981 1038 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1263 1605 1263
Size: 342 4124
EcoRI G'AATT_C
Cuts at: 1470 1470
Size: 4466
EcoRV GAT'ATC
Cuts at: 339 977 339
Size: 638 3828
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 4466
FspI TGC'GCA
Cuts at: 2247 2247
Size: 4466
HaeII r_GCGC'y
Cuts at: 163 504 1900 2148 3170 4149 163
Size: 341 1396 248 1022 979 480
Fragments arranged by size:
1396 1022 979 480 341 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 130 162 615 647 1315 1347 2308 2338 2340
Size: 32 453 32 668 32 961 30 2
Cuts at: 2340 2370 130
Size: 30 2226
Fragments arranged by size:
2226 961 668 453 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1505 1505
Size: 4466
HindIII A'AGCT_T
Cuts at: 242 242
Size: 4466
KasI G'GCGC_C
Cuts at: 1896 2144 1896
Size: 248 4218
KpnI G_GTAC'C
Cuts at: 1486 1486
Size: 4466
MfeI C'AATT_G
Cuts at: 1100 1100
Size: 4466
MluI A'CGCG_T
Cuts at: 3899 3899
Size: 4466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1138 3137 3321 1138
Size: 1999 184 2283
Fragments arranged by size:
2283 1999 184
MscI TGG'CCA
Cuts at: 313 2227 313
Size: 1914 2552
MslI CAynn'nnrTG
Cuts at: 892 1070 1150 2455 2582 892
Size: 178 80 1305 127 2776
Fragments arranged by size:
2776 1305 178 127 80
MspA1I CmG'CkG
Cuts at: 1577 1891 2251 3264 3509 1577
Size: 314 360 1013 245 2534
Fragments arranged by size:
2534 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2647 2647
Size: 4466
NarI GG'CG_CC
Cuts at: 1897 2145 1897
Size: 248 4218
NcoI C'CATG_G
Cuts at: 107 558 107
Size: 451 4015
NdeI CA'TA_TG
Cuts at: 1239 1239
Size: 4466
NgoMIV G'CCGG_C
Cuts at: 2645 2645
Size: 4466
NsiI A_TGCA'T
Cuts at: 309 309
Size: 4466
NspI r_CATG'y
Cuts at: 1519 2019 2926 1519
Size: 500 907 3059
Fragments arranged by size:
3059 907 500
PasI CC'CwG_GG
Cuts at: 1423 1423
Size: 4466
PciI A'CATG_T
Cuts at: 2922 2922
Size: 4466
PflMI CCAn_nnn'nTGG
Cuts at: 333 333
Size: 4466
PmeI GTTT'AAAC
Cuts at: 1465 1465
Size: 4466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 618 650 3605 3637 618
Size: 32 2955 32 1447
Fragments arranged by size:
2955 1447 32 32
PsiI TTA'TAA
Cuts at: 701 1363 701
Size: 662 3804
PspOMI G'GGCC_C
Cuts at: 1262 1262
Size: 4466
PstI C_TGCA'G
Cuts at: 122 1218 1513 2198 122
Size: 1096 295 685 2390
Fragments arranged by size:
2390 1096 685 295
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4466
PvuII CAG'CTG
Cuts at: 1891 2251 1891
Size: 360 4106
RsrII CG'GwC_CG
Cuts at: 2661 2661
Size: 4466
SacI G_AGCT'C
Cuts at: 1480 1480
Size: 4466
SalI G'TCGA_C
Cuts at: 1503 1503
Size: 4466
SapI GCTCTTCn'nnn_
Cuts at: 2489 2699 2489
Size: 210 4256
SbfI CC_TGCA'GG
Cuts at: 1513 1513
Size: 4466
ScaI AGT'ACT
Cuts at: 3983 3983
Size: 4466
SfcI C'TryA_G
Cuts at: 33 118 227 1214 1509 2194 3187 3378 33
Size: 85 109 987 295 685 993 191 1121
Fragments arranged by size:
1121 993 987 685 295 191 109 85
SfoI GGC'GCC
Cuts at: 1898 2146 1898
Size: 248 4218
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
SmaI CCC'GGG
Cuts at: 932 1488 932
Size: 556 3910
SmlI C'TyrA_G
Cuts at: 1621 2536 3028 3290 1621
Size: 915 492 262 2797
Fragments arranged by size:
2797 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4466
SphI G_CATG'C
Cuts at: 1519 2019 1519
Size: 500 3966
StyI C'CwwG_G
Cuts at: 107 558 814 1600 1954 107
Size: 451 256 786 354 2619
Fragments arranged by size:
2619 786 451 354 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 927 2049 2182 2641 2850 927
Size: 1122 133 459 209 2543
Fragments arranged by size:
2543 1122 459 209 133
TatI w'GTAC_w
Cuts at: 321 3981 321
Size: 3660 806
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 810 842 3983 4015 810
Size: 32 3141 32 1261
Fragments arranged by size:
3141 1261 32 32
Tth111I GACn'n_nGTC
Cuts at: 2263 2263
Size: 4466
XbaI T'CTAG_A
Cuts at: 1497 1497
Size: 4466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1961 1961
Size: 4466
XmaI C'CCGG_G
Cuts at: 930 1486 930
Size: 556 3910
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PmeI PpiI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BbvCI BglI BmgBI BmtI Bpu10I BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I CspCI
EcoNI FseI FspAI HpaI NheI NotI NruI PacI
PfoI PmlI PpuMI PshAI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI