Restriction map for
pF1KB4099
(Circular) (Six-base) MAPSORT of: pF1KB4099.seq Check: 5086 from: 1 to: 4469
pF1KB4099 4469 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1293 1293
Size: 4469
AccI GT'mk_AC
Cuts at: 1507 1507
Size: 4469
Acc65I G'GTAC_C
Cuts at: 1485 1485
Size: 4469
AclI AA'CG_TT
Cuts at: 323 4309 323
Size: 3986 483
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 224 767 2294 2726 3473 3741 156
Size: 68 543 1527 432 747 268 884
Fragments arranged by size:
1527 884 747 543 432 268 68
AflIII A'CryG_T
Cuts at: 1821 2925 3902 1821
Size: 1104 977 2388
Fragments arranged by size:
2388 1104 977
AgeI A'CCGG_T
Cuts at: 2857 2857
Size: 4469
AhdI GACnn_n'nnGTC
Cuts at: 204 204
Size: 4469
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1114 1146 1114
Size: 32 4437
AlwNI CAG_nnn'CTG
Cuts at: 958 1774 3341 958
Size: 816 1567 2086
Fragments arranged by size:
2086 1567 816
ApaLI G'TGCA_C
Cuts at: 3239 3239
Size: 4469
ApoI r'AATT_y
Cuts at: 300 1473 3822 4078 300
Size: 1173 2349 256 691
Fragments arranged by size:
2349 1173 691 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4469
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
AvaI C'yCGr_G
Cuts at: 1373 1489 1373
Size: 116 4353
BamHI G'GATC_C
Cuts at: 1494 1494
Size: 4469
BanI G'GyrC_C
Cuts at: 523 1485 1899 2147 2182 523
Size: 962 414 248 35 2810
Fragments arranged by size:
2810 962 414 248 35
BanII G_rGCy'C
Cuts at: 1483 2513 1483
Size: 1030 3439
BbeI G_GCGC'C
Cuts at: 1903 2151 1903
Size: 248 4221
BbsI GAAGACnn'nnnn_
Cuts at: 1173 1199 1173
Size: 26 4443
BciVI GTATCCnnnnn_n'
Cuts at: 2367 3128 4469 2367
Size: 761 1341 2367
Fragments arranged by size:
2367 1341 761
BclI T'GATC_A
Cuts at: 1989 1989
Size: 4469
BglII A'GATC_T
Cuts at: 1984 4021 1984
Size: 2037 2432
BlpI GC'TnA_GC
Cuts at: 1581 1581
Size: 4469
Bme1580I G_kGCm'C
Cuts at: 2094 2187 3243 2094
Size: 93 1056 3320
Fragments arranged by size:
3320 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 759 982 1681 1762 2097 4241 759
Size: 223 699 81 335 2144 987
Fragments arranged by size:
2144 987 699 335 223 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2836 2836
Size: 4469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 299 697 1002 1116 186
Size: 113 398 305 114 3539
Fragments arranged by size:
3539 398 305 114 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1645 2524 3016 3314 1645
Size: 879 492 298 2800
Fragments arranged by size:
2800 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1044 4173 1044
Size: 3129 1340
BsaAI yAC'GTr
Cuts at: 1822 1822
Size: 4469
BsaBI GATnn'nnATC
Cuts at: 6 892 1060 1983 4020 6
Size: 886 168 923 2037 455
Fragments arranged by size:
2037 923 886 455 168
BsaHI Gr'CG_yC
Cuts at: 1900 2148 2850 4346 1900
Size: 248 702 1496 2023
Fragments arranged by size:
2023 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1658 2179 2857 3131 3278 3689 1658
Size: 521 678 274 147 411 2438
Fragments arranged by size:
2438 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 184 214 219 249 1114 1144 184
Size: 30 5 30 865 30 3509
Fragments arranged by size:
3509 865 30 30 30 5
BseYI C'CCAG_C
Cuts at: 1894 2652 3229 3725 1894
Size: 758 577 496 2638
Fragments arranged by size:
2638 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2011 2057 3265 104
Size: 1907 46 1208 1308
Fragments arranged by size:
1907 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 590 1483 2261 2451 3243 590
Size: 893 778 190 792 1816
Fragments arranged by size:
1816 893 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1134 1134
Size: 4469
Bsp1286I G_dGCh'C
Cuts at: 590 1483 2094 2187 2261 2451 2513 3243 590
Size: 893 611 93 74 190 62 730 1816
Fragments arranged by size:
1816 893 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3689 3689
Size: 4469
BspHI T'CATG_A
Cuts at: 3645 3645
Size: 4469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 841 1293 1519 1733 2035 2416 841
Size: 452 226 214 302 381 2894
Fragments arranged by size:
2894 452 381 302 226 214
BsrBI CCG'CTC
Cuts at: 50 2761 2815 4299 4464 50
Size: 2711 54 1484 165 55
Fragments arranged by size:
2711 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2381 2381
Size: 4469
BsrFI r'CCGG_y
Cuts at: 113 2467 2648 2857 113
Size: 2354 181 209 1725
Fragments arranged by size:
2354 1725 209 181
BsrGI T'GTAC_A
Cuts at: 664 802 890 664
Size: 138 88 4243
Fragments arranged by size:
4243 138 88
BssSI C'ACGA_G
Cuts at: 1202 2740 3098 1202
Size: 1538 358 2573
Fragments arranged by size:
2573 1538 358
BstAPI GCAn_nnn'nTGC
Cuts at: 958 1846 958
Size: 888 3581
BstBI TT'CG_AA
Cuts at: 571 2830 571
Size: 2259 2210
BstYI r'GATC_y
Cuts at: 1 1494 1961 1984 2319 2565 3577 3663 3675
Size: 1493 467 23 335 246 1012 86 12
Cuts at: 3675 4021 1
Size: 346 449
Fragments arranged by size:
1493 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 374 626 374
Size: 252 4217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2419 2600 2763 2419
Size: 181 163 4125
Fragments arranged by size:
4125 181 163
BtsI GCAGTG_nn'
Cuts at: 1242 1712 1242
Size: 470 3999
DraI TTT'AAA
Cuts at: 306 1468 306
Size: 1162 3307
DrdI GACnn_nn'nnGTC
Cuts at: 2175 3033 2175
Size: 858 3611
EaeI y'GGCC_r
Cuts at: 2054 2228 2619 2646 3991 2054
Size: 174 391 27 1345 2532
Fragments arranged by size:
2532 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2054 2054
Size: 4469
EarI CTCTTCn'nnn_
Cuts at: 776 1168 2492 2702 776
Size: 392 1324 210 2543
Fragments arranged by size:
2543 1324 392 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2677 2987 3133 4279 2677
Size: 310 146 1146 2867
Fragments arranged by size:
2867 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1481 1481
Size: 4469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 186 224 299 697 767 1002 1116 2294
Size: 30 38 75 398 70 235 114 1178
Cuts at: 2294 2726 3473 3741 156
Size: 432 747 268 884
Fragments arranged by size:
1178 884 747 432 398 268 235 114
75 70 38 30
EcoO109I rG'GnC_Cy
Cuts at: 310 1608 310
Size: 1298 3171
EcoRI G'AATT_C
Cuts at: 1473 1473
Size: 4469
EcoRV GAT'ATC
Cuts at: 1370 1370
Size: 4469
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4469
FspI TGC'GCA
Cuts at: 2250 2250
Size: 4469
HaeII r_GCGC'y
Cuts at: 1903 2151 3173 4152 1903
Size: 248 1022 979 2220
Fragments arranged by size:
2220 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 190 216 219 222 251 1310 1342 1354
Size: 6 26 3 3 29 1059 32 12
Cuts at: 1354 1386 2311 2341 2343 2373 184
Size: 32 925 30 2 30 2280
Fragments arranged by size:
2280 1059 925 32 32 30 30 29
26 12 6 3 3 2
HincII GTy'rAC
Cuts at: 1508 1508
Size: 4469
KasI G'GCGC_C
Cuts at: 1899 2147 1899
Size: 248 4221
KpnI G_GTAC'C
Cuts at: 1489 1489
Size: 4469
MluI A'CGCG_T
Cuts at: 3902 3902
Size: 4469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3140 3324 3140
Size: 184 4285
MscI TGG'CCA
Cuts at: 2230 2230
Size: 4469
MslI CAynn'nnrTG
Cuts at: 1066 1448 2458 2585 1066
Size: 382 1010 127 2950
Fragments arranged by size:
2950 1010 382 127
MspA1I CmG'CkG
Cuts at: 675 1117 1270 1580 1894 2254 3267 3512 675
Size: 442 153 310 314 360 1013 245 1632
Fragments arranged by size:
1632 1013 442 360 314 310 245 153
NaeI GCC'GGC
Cuts at: 2650 2650
Size: 4469
NarI GG'CG_CC
Cuts at: 1900 2148 1900
Size: 248 4221
NcoI C'CATG_G
Cuts at: 374 626 374
Size: 252 4217
NgoMIV G'CCGG_C
Cuts at: 2648 2648
Size: 4469
NspI r_CATG'y
Cuts at: 1522 2022 2929 1522
Size: 500 907 3062
Fragments arranged by size:
3062 907 500
PciI A'CATG_T
Cuts at: 2925 2925
Size: 4469
PflMI CCAn_nnn'nTGG
Cuts at: 1315 1315
Size: 4469
PmeI GTTT'AAAC
Cuts at: 1468 1468
Size: 4469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1114 1146 3608 3640 1114
Size: 32 2462 32 1943
Fragments arranged by size:
2462 1943 32 32
PpuMI rG'GwC_Cy
Cuts at: 310 310
Size: 4469
PstI C_TGCA'G
Cuts at: 1516 2201 1516
Size: 685 3784
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4469
PvuII CAG'CTG
Cuts at: 675 1270 1894 2254 675
Size: 595 624 360 2890
Fragments arranged by size:
2890 624 595 360
RsrII CG'GwC_CG
Cuts at: 2664 2664
Size: 4469
SacI G_AGCT'C
Cuts at: 1483 1483
Size: 4469
SalI G'TCGA_C
Cuts at: 1506 1506
Size: 4469
SapI GCTCTTCn'nnn_
Cuts at: 2492 2702 2492
Size: 210 4259
SbfI CC_TGCA'GG
Cuts at: 1516 1516
Size: 4469
ScaI AGT'ACT
Cuts at: 3986 3986
Size: 4469
SfcI C'TryA_G
Cuts at: 33 591 1512 2197 3190 3381 33
Size: 558 921 685 993 191 1121
Fragments arranged by size:
1121 993 921 685 558 191
SfoI GGC'GCC
Cuts at: 1901 2149 1901
Size: 248 4221
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
SmaI CCC'GGG
Cuts at: 1491 1491
Size: 4469
SmlI C'TyrA_G
Cuts at: 1373 1624 2539 3031 3293 1373
Size: 251 915 492 262 2549
Fragments arranged by size:
2549 915 492 262 251
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4469
SphI G_CATG'C
Cuts at: 1522 2022 1522
Size: 500 3969
SspI AAT'ATT
Cuts at: 336 393 336
Size: 57 4412
StyI C'CwwG_G
Cuts at: 374 626 1603 1957 374
Size: 252 977 354 2886
Fragments arranged by size:
2886 977 354 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1018 2052 2185 2644 2853 1018
Size: 1034 133 459 209 2634
Fragments arranged by size:
2634 1034 459 209 133
TatI w'GTAC_w
Cuts at: 664 802 890 3984 664
Size: 138 88 3094 1149
Fragments arranged by size:
3094 1149 138 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3986 4018 3986
Size: 32 4437
Tth111I GACn'n_nGTC
Cuts at: 2266 2266
Size: 4469
XbaI T'CTAG_A
Cuts at: 1500 1500
Size: 4469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 969 1258 1964 969
Size: 289 706 3474
Fragments arranged by size:
3474 706 289
XhoI C'TCGA_G
Cuts at: 1373 1373
Size: 4469
XmaI C'CCGG_G
Cuts at: 1489 1489
Size: 4469
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BseRI BsgI BsiWI BsmI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XmnI ZraI