Restriction map for
pF1KB4098
(Circular) (Six-base) MAPSORT of: pF1KB4098.seq Check: 9966 from: 1 to: 4346
pF1KB4098 4346 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1384 1384
Size: 4346
Acc65I G'GTAC_C
Cuts at: 1362 1362
Size: 4346
AclI AA'CG_TT
Cuts at: 4186 4186
Size: 4346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 341 2171 2603 3350 3618 334
Size: 7 1830 432 747 268 1062
Fragments arranged by size:
1830 1062 747 432 268 7
AfeI AGC'GCT
Cuts at: 138 138
Size: 4346
AflIII A'CryG_T
Cuts at: 1698 2802 3779 1698
Size: 1104 977 2265
Fragments arranged by size:
2265 1104 977
AgeI A'CCGG_T
Cuts at: 2734 2734
Size: 4346
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 983 1015 983
Size: 32 4314
AlwNI CAG_nnn'CTG
Cuts at: 228 393 1651 3218 228
Size: 165 1258 1567 1356
Fragments arranged by size:
1567 1356 1258 165
ApaLI G'TGCA_C
Cuts at: 3116 3116
Size: 4346
ApoI r'AATT_y
Cuts at: 409 805 1350 3699 3955 409
Size: 396 545 2349 256 800
Fragments arranged by size:
2349 800 545 396 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4346
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
AvaI C'yCGr_G
Cuts at: 170 1366 170
Size: 1196 3150
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1278 1311 1278
Size: 33 4313
BamHI G'GATC_C
Cuts at: 1371 1371
Size: 4346
BanI G'GyrC_C
Cuts at: 154 165 1362 1776 2024 2059 154
Size: 11 1197 414 248 35 2441
Fragments arranged by size:
2441 1197 414 248 35 11
BanII G_rGCy'C
Cuts at: 1276 1360 2390 1276
Size: 84 1030 3232
Fragments arranged by size:
3232 1030 84
BbeI G_GCGC'C
Cuts at: 158 1780 2028 158
Size: 1622 248 2476
Fragments arranged by size:
2476 1622 248
BbsI GAAGACnn'nnnn_
Cuts at: 968 1202 968
Size: 234 4112
BciVI GTATCCnnnnn_n'
Cuts at: 1306 2244 3005 4346 1306
Size: 938 761 1341 1306
Fragments arranged by size:
1341 1306 938 761
BclI T'GATC_A
Cuts at: 753 1866 753
Size: 1113 3233
BfrBI ATG'CAT
Cuts at: 250 250
Size: 4346
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4346
BglII A'GATC_T
Cuts at: 1146 1861 3898 1146
Size: 715 2037 1594
Fragments arranged by size:
2037 1594 715
BlpI GC'TnA_GC
Cuts at: 1458 1458
Size: 4346
Bme1580I G_kGCm'C
Cuts at: 170 1971 2064 3120 170
Size: 1801 93 1056 1396
Fragments arranged by size:
1801 1396 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 547 1558 1639 1974 4118 547
Size: 1011 81 335 2144 775
Fragments arranged by size:
2144 1011 775 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 204 2713 204
Size: 2509 1837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 758 758
Size: 4346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1522 2401 2893 3191 1522
Size: 879 492 298 2677
Fragments arranged by size:
2677 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4050 4050
Size: 4346
BsaAI yAC'GTr
Cuts at: 1699 1699
Size: 4346
BsaBI GATnn'nnATC
Cuts at: 6 1151 1860 3897 6
Size: 1145 709 2037 455
Fragments arranged by size:
2037 1145 709 455
BsaHI Gr'CG_yC
Cuts at: 155 1777 2025 2727 4223 155
Size: 1622 248 702 1496 278
Fragments arranged by size:
1622 1496 702 278 248
BsaWI w'CCGG_w
Cuts at: 1535 2056 2734 3008 3155 3566 1535
Size: 521 678 274 147 411 2315
Fragments arranged by size:
2315 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 983 1013 983
Size: 30 4316
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1004 1004
Size: 4346
BseYI C'CCAG_C
Cuts at: 1771 2529 3106 3602 1771
Size: 758 577 496 2515
Fragments arranged by size:
2515 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1031 1031
Size: 4346
BsiEI CG_ry'CG
Cuts at: 104 1888 1934 3142 104
Size: 1784 46 1208 1308
Fragments arranged by size:
1784 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1276 1360 2138 2328 3120 1276
Size: 84 778 190 792 2502
Fragments arranged by size:
2502 792 778 190 84
BsmI GAATG_Cn'
Cuts at: 885 1083 885
Size: 198 4148
Bsp1286I G_dGCh'C
Cuts at: 170 1276 1360 1971 2064 2138 2328 2390 3120
Size: 1106 84 611 93 74 190 62 730
Cuts at: 3120 170
Size: 1396
Fragments arranged by size:
1396 1106 730 611 190 93 84 74
62
BspEI T'CCGG_A
Cuts at: 3566 3566
Size: 4346
BspHI T'CATG_A
Cuts at: 3522 3522
Size: 4346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1396 1610 1912 2293 1396
Size: 214 302 381 3449
Fragments arranged by size:
3449 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2638 2692 4176 4341 50
Size: 2588 54 1484 165 55
Fragments arranged by size:
2588 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 890 2258 890
Size: 1368 2978
BsrFI r'CCGG_y
Cuts at: 157 2344 2525 2734 157
Size: 2187 181 209 1769
Fragments arranged by size:
2187 1769 209 181
BsrGI T'GTAC_A
Cuts at: 541 541
Size: 4346
BssSI C'ACGA_G
Cuts at: 2617 2975 2617
Size: 358 3988
BstAPI GCAn_nnn'nTGC
Cuts at: 1723 1723
Size: 4346
BstBI TT'CG_AA
Cuts at: 477 2707 477
Size: 2230 2116
BstYI r'GATC_y
Cuts at: 1 1146 1371 1838 1861 2196 2442 3454 3540
Size: 1145 225 467 23 335 246 1012 86
Cuts at: 3540 3552 3898 1
Size: 12 346 449
Fragments arranged by size:
1145 1012 467 449 346 335 246 225
86 23 12
BtgI C'CryG_G
Cuts at: 1337 1337
Size: 4346
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2296 2477 2640 2296
Size: 181 163 4002
Fragments arranged by size:
4002 181 163
BtsI GCAGTG_nn'
Cuts at: 719 1043 1589 719
Size: 324 546 3476
Fragments arranged by size:
3476 546 324
ClaI AT'CG_AT
Cuts at: 588 588
Size: 4346
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1313 1348 1313
Size: 35 4311
DraI TTT'AAA
Cuts at: 1345 1345
Size: 4346
DraIII CAC_nnn'GTG
Cuts at: 831 831
Size: 4346
DrdI GACnn_nn'nnGTC
Cuts at: 2052 2910 2052
Size: 858 3488
EaeI y'GGCC_r
Cuts at: 1334 1931 2105 2496 2523 3868 1334
Size: 597 174 391 27 1345 1812
Fragments arranged by size:
1812 1345 597 391 174 27
EagI C'GGCC_G
Cuts at: 1931 1931
Size: 4346
EarI CTCTTCn'nnn_
Cuts at: 360 2369 2579 360
Size: 2009 210 2127
Fragments arranged by size:
2127 2009 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2554 2864 3010 4156 2554
Size: 310 146 1146 2744
Fragments arranged by size:
2744 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1274 1358 1274
Size: 84 4262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 341 758 2171 2603 3350 3618 334
Size: 7 417 1413 432 747 268 1062
Fragments arranged by size:
1413 1062 747 432 417 268 7
EcoO109I rG'GnC_Cy
Cuts at: 1485 1485
Size: 4346
EcoRI G'AATT_C
Cuts at: 409 1350 409
Size: 941 3405
EcoRV GAT'ATC
Cuts at: 327 327
Size: 4346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 281 982 1269 2365 281
Size: 701 287 1096 2262
Fragments arranged by size:
2262 1096 701 287
FspI TGC'GCA
Cuts at: 133 2127 133
Size: 1994 2352
HaeII r_GCGC'y
Cuts at: 140 158 1780 2028 3050 4029 140
Size: 18 1622 248 1022 979 457
Fragments arranged by size:
1622 1022 979 457 248 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2188 2218 2220 2250 2188
Size: 30 2 30 4284
Fragments arranged by size:
4284 30 30 2
HincII GTy'rAC
Cuts at: 549 1385 549
Size: 836 3510
HindIII A'AGCT_T
Cuts at: 784 784
Size: 4346
KasI G'GCGC_C
Cuts at: 154 1776 2024 154
Size: 1622 248 2476
Fragments arranged by size:
2476 1622 248
KpnI G_GTAC'C
Cuts at: 1366 1366
Size: 4346
MfeI C'AATT_G
Cuts at: 364 364
Size: 4346
MluI A'CGCG_T
Cuts at: 3779 3779
Size: 4346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3017 3201 3017
Size: 184 4162
MscI TGG'CCA
Cuts at: 1336 2107 1336
Size: 771 3575
MslI CAynn'nnrTG
Cuts at: 491 2335 2462 491
Size: 1844 127 2375
Fragments arranged by size:
2375 1844 127
MspA1I CmG'CkG
Cuts at: 210 1457 1771 2131 3144 3389 210
Size: 1247 314 360 1013 245 1167
Fragments arranged by size:
1247 1167 1013 360 314 245
NaeI GCC'GGC
Cuts at: 159 2527 159
Size: 2368 1978
NarI GG'CG_CC
Cuts at: 155 1777 2025 155
Size: 1622 248 2476
Fragments arranged by size:
2476 1622 248
NcoI C'CATG_G
Cuts at: 1337 1337
Size: 4346
NdeI CA'TA_TG
Cuts at: 857 857
Size: 4346
NgoMIV G'CCGG_C
Cuts at: 157 2525 157
Size: 2368 1978
NsiI A_TGCA'T
Cuts at: 252 252
Size: 4346
NspI r_CATG'y
Cuts at: 254 1399 1899 2806 254
Size: 1145 500 907 1794
Fragments arranged by size:
1794 1145 907 500
PciI A'CATG_T
Cuts at: 2802 2802
Size: 4346
PfoI T'CCnGG_A
Cuts at: 1072 1072
Size: 4346
PmeI GTTT'AAAC
Cuts at: 1345 1345
Size: 4346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 983 1015 3485 3517 983
Size: 32 2470 32 1812
Fragments arranged by size:
2470 1812 32 32
PstI C_TGCA'G
Cuts at: 1393 2078 1393
Size: 685 3661
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4346
PvuII CAG'CTG
Cuts at: 1771 2131 1771
Size: 360 3986
RsrII CG'GwC_CG
Cuts at: 2541 2541
Size: 4346
SacI G_AGCT'C
Cuts at: 1276 1360 1276
Size: 84 4262
SalI G'TCGA_C
Cuts at: 1383 1383
Size: 4346
SapI GCTCTTCn'nnn_
Cuts at: 2369 2579 2369
Size: 210 4136
SbfI CC_TGCA'GG
Cuts at: 1393 1393
Size: 4346
ScaI AGT'ACT
Cuts at: 1135 3863 1135
Size: 2728 1618
SfcI C'TryA_G
Cuts at: 33 195 1220 1389 2074 3067 3258 33
Size: 162 1025 169 685 993 191 1121
Fragments arranged by size:
1121 1025 993 685 191 169 162
SfoI GGC'GCC
Cuts at: 156 1778 2026 156
Size: 1622 248 2476
Fragments arranged by size:
2476 1622 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
SmaI CCC'GGG
Cuts at: 1368 1368
Size: 4346
SmlI C'TyrA_G
Cuts at: 1501 2416 2908 3170 1501
Size: 915 492 262 2677
Fragments arranged by size:
2677 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4346
SphI G_CATG'C
Cuts at: 1399 1899 1399
Size: 500 3846
SspI AAT'ATT
Cuts at: 577 577
Size: 4346
StyI C'CwwG_G
Cuts at: 1337 1480 1834 1337
Size: 143 354 3849
Fragments arranged by size:
3849 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1929 2062 2521 2730 1929
Size: 133 459 209 3545
Fragments arranged by size:
3545 459 209 133
TatI w'GTAC_w
Cuts at: 541 1133 3861 541
Size: 592 2728 1026
Fragments arranged by size:
2728 1026 592
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 735 767 3863 3895 735
Size: 32 3096 32 1186
Fragments arranged by size:
3096 1186 32 32
Tth111I GACn'n_nGTC
Cuts at: 553 2143 553
Size: 1590 2756
XbaI T'CTAG_A
Cuts at: 1377 1377
Size: 4346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1841 1841
Size: 4346
XmaI C'CCGG_G
Cuts at: 1366 1366
Size: 4346
XmnI GAAnn'nnTTC
Cuts at: 1229 1229
Size: 4346
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI ApaI AscI
AvrII BbvCI BcgI BmgBI BmtI Bpu10I BsiWI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I EcoNI FseI FspAI
HpaI NheI NotI NruI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI