Restriction map for
pF1KB4094
(Circular) (Six-base) MAPSORT of: pF1KB4094.seq Check: 1727 from: 1 to: 4958
pF1KB4094 4958 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1367 1367
Size: 4958
AccI GT'mk_AC
Cuts at: 410 1996 410
Size: 1586 3372
Acc65I G'GTAC_C
Cuts at: 947 1974 947
Size: 1027 3931
AclI AA'CG_TT
Cuts at: 278 4798 278
Size: 4520 438
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 522 1270 1730 2783 3215 3568 4230 136
Size: 386 748 460 1053 432 353 662 864
Fragments arranged by size:
1053 864 748 662 460 432 386 353
AflIII A'CryG_T
Cuts at: 1085 2310 4110 4391 1085
Size: 1225 1800 281 1652
Fragments arranged by size:
1800 1652 1225 281
AleI CACnn'nnGTG
Cuts at: 1411 1411
Size: 4958
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 817 849 817
Size: 32 4926
AlwNI CAG_nnn'CTG
Cuts at: 624 909 2263 3701 624
Size: 285 1354 1438 1881
Fragments arranged by size:
1881 1438 1354 285
ApaI G_GGCC'C
Cuts at: 522 686 1570 522
Size: 164 884 3910
Fragments arranged by size:
3910 884 164
ApaLI G'TGCA_C
Cuts at: 3796 3796
Size: 4958
ApoI r'AATT_y
Cuts at: 1962 4311 4567 1962
Size: 2349 256 2353
Fragments arranged by size:
2353 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4958
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4958
AvaI C'yCGr_G
Cuts at: 283 428 1547 1700 1836 1978 283
Size: 145 1119 153 136 142 3263
Fragments arranged by size:
3263 1119 153 145 142 136
AvrII C'CTAG_G
Cuts at: 1452 1452
Size: 4958
BamHI G'GATC_C
Cuts at: 1983 1983
Size: 4958
BanI G'GyrC_C
Cuts at: 403 568 947 1309 1636 1731 1974 2388 2636
Size: 165 379 362 327 95 243 414 248
Cuts at: 2636 2671 403
Size: 35 2690
Fragments arranged by size:
2690 414 379 362 327 248 243 165
95 35
BanII G_rGCy'C
Cuts at: 290 522 562 686 995 1570 1598 1972 3002
Size: 232 40 124 309 575 28 374 1030
Cuts at: 3002 290
Size: 2246
Fragments arranged by size:
2246 1030 575 374 309 232 124 40
28
BbeI G_GCGC'C
Cuts at: 407 2392 2640 407
Size: 1985 248 2725
Fragments arranged by size:
2725 1985 248
BbsI GAAGACnn'nnnn_
Cuts at: 136 1196 136
Size: 1060 3898
BbvCI CC'TCA_GC
Cuts at: 493 704 1044 493
Size: 211 340 4407
Fragments arranged by size:
4407 340 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1932 1966 1932
Size: 34 4924
BciVI GTATCCnnnnn_n'
Cuts at: 2856 3912 4958 2856
Size: 1056 1046 2856
Fragments arranged by size:
2856 1056 1046
BclI T'GATC_A
Cuts at: 2478 2478
Size: 4958
BglI GCCn_nnn'nGGC
Cuts at: 566 619 766 1138 1513 566
Size: 53 147 372 375 4011
Fragments arranged by size:
4011 375 372 147 53
BglII A'GATC_T
Cuts at: 975 1199 2473 4510 975
Size: 224 1274 2037 1423
Fragments arranged by size:
2037 1423 1274 224
BlpI GC'TnA_GC
Cuts at: 770 2070 770
Size: 1300 3658
Bme1580I G_kGCm'C
Cuts at: 522 686 1312 1570 1639 2583 2676 3800 522
Size: 164 626 258 69 944 93 1124 1680
Fragments arranged by size:
1680 1124 944 626 258 164 93 69
BmgBI CAC'GTC
Cuts at: 162 162
Size: 4958
BmrI ACTGGGnnnn_n'
Cuts at: 556 2170 2251 2586 4730 556
Size: 1614 81 335 2144 784
Fragments arranged by size:
2144 1614 784 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3325 3325
Size: 4958
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 904 1009 1291 1318 429
Size: 475 105 282 27 4069
Fragments arranged by size:
4069 475 282 105 27
Bpu10I CC'TnA_GC
Cuts at: 493 704 1044 1727 493
Size: 211 340 683 3724
Fragments arranged by size:
3724 683 340 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1915 2134 3013 3727 4025 1915
Size: 219 879 714 298 2848
Fragments arranged by size:
2848 879 714 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 474 634 4662 474
Size: 160 4028 770
Fragments arranged by size:
4028 770 160
BsaAI yAC'GTr
Cuts at: 1422 2311 1422
Size: 889 4069
BsaBI GATnn'nnATC
Cuts at: 6 2472 4509 6
Size: 2466 2037 455
Fragments arranged by size:
2466 2037 455
BsaHI Gr'CG_yC
Cuts at: 404 2389 2637 3339 4835 404
Size: 1985 248 702 1496 527
Fragments arranged by size:
1985 1496 702 527 248
BsaWI w'CCGG_w
Cuts at: 586 2147 2668 3346 3757 3904 4178 586
Size: 1561 521 678 411 147 274 1366
Fragments arranged by size:
1561 1366 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 817 847 817
Size: 30 4928
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 997 1210 1297 1408 1540 1606 211
Size: 786 213 87 111 132 66 3563
Fragments arranged by size:
3563 786 213 132 111 87 66
BseYI C'CCAG_C
Cuts at: 186 228 301 1427 2383 3141 3806 4214 186
Size: 42 73 1126 956 758 665 408 930
Fragments arranged by size:
1126 956 930 758 665 408 73 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 1402 1465 1670 1897 853
Size: 549 63 205 227 3914
Fragments arranged by size:
3914 549 227 205 63
BsiEI CG_ry'CG
Cuts at: 104 1291 1517 2500 2546 3776 104
Size: 1187 226 983 46 1230 1286
Fragments arranged by size:
1286 1230 1187 983 226 46
BsiHKAI G_wGCw'C
Cuts at: 215 290 1775 1972 2750 2940 3800 215
Size: 75 1485 197 778 190 860 1373
Fragments arranged by size:
1485 1373 860 778 197 190 75
Bsp1286I G_dGCh'C
Cuts at: 215 290 522 562 686 995 1312 1570 1598
Size: 75 232 40 124 309 317 258 28
Cuts at: 1598 1639 1775 1972 2583 2676 2750 2940 3002
Size: 41 136 197 611 93 74 190 62
Cuts at: 3002 3800 215
Size: 798 1373
Fragments arranged by size:
1373 798 611 317 309 258 232 197
190 136 124 93 75 74 62 41
40 28
BspHI T'CATG_A
Cuts at: 3390 3390
Size: 4958
BspMI ACCTGCnnnn'nnnn_
Cuts at: 875 1253 1367 2008 2222 2524 2905 875
Size: 378 114 641 214 302 381 2928
Fragments arranged by size:
2928 641 381 378 302 214 114
BsrBI CCG'CTC
Cuts at: 50 500 774 1287 3250 3304 4788 4953 50
Size: 450 274 513 1963 54 1484 165 55
Fragments arranged by size:
1963 1484 513 450 274 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1037 1125 2870 1037
Size: 88 1745 3125
Fragments arranged by size:
3125 1745 88
BsrFI r'CCGG_y
Cuts at: 760 1004 1556 1639 2956 3137 760
Size: 244 552 83 1317 181 2581
Fragments arranged by size:
2581 1317 552 244 181 83
BssSI C'ACGA_G
Cuts at: 3229 3937 3229
Size: 708 4250
BstAPI GCAn_nnn'nTGC
Cuts at: 909 1485 2335 909
Size: 576 850 3532
Fragments arranged by size:
3532 850 576
BstBI TT'CG_AA
Cuts at: 3319 3319
Size: 4958
BstEII G'GTnAC_C
Cuts at: 708 708
Size: 4958
BstXI CCAn_nnnn'nTGG
Cuts at: 1269 1269
Size: 4958
BstYI r'GATC_y
Cuts at: 1 395 975 1199 1983 2450 2473 2808 3054
Size: 394 580 224 784 467 23 335 246
Cuts at: 3054 3360 3372 3458 4510 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 784 580 467 449 394 335 306
246 224 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 960 960
Size: 4958
BtgI C'CryG_G
Cuts at: 107 344 953 1531 1570 107
Size: 237 609 578 39 3495
Fragments arranged by size:
3495 609 578 237 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 348 2908 3089 3252 348
Size: 2560 181 163 2054
Fragments arranged by size:
2560 2054 181 163
BtsI GCAGTG_nn'
Cuts at: 1658 2201 1658
Size: 543 4415
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1422 1457 1422
Size: 35 4923
DraI TTT'AAA
Cuts at: 1957 1957
Size: 4958
DrdI GACnn_nn'nnGTC
Cuts at: 2664 4008 2664
Size: 1344 3614
EaeI y'GGCC_r
Cuts at: 347 1002 1006 1154 1288 1514 1641 1820 2543
Size: 655 4 148 134 226 127 179 723
Cuts at: 2543 2717 3108 3135 4480 347
Size: 174 391 27 1345 825
Fragments arranged by size:
1345 825 723 655 391 226 179 174
148 134 127 27 4
EagI C'GGCC_G
Cuts at: 1288 1514 2543 1288
Size: 226 1029 3703
Fragments arranged by size:
3703 1029 226
EarI CTCTTCn'nnn_
Cuts at: 541 2981 3191 541
Size: 2440 210 2308
Fragments arranged by size:
2440 2308 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1162 1317 3166 3908 4054 4768 1162
Size: 155 1849 742 146 714 1352
Fragments arranged by size:
1849 1352 742 714 155 146
EcoICRI GAG'CTC
Cuts at: 288 1970 288
Size: 1682 3276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 429 522 904 1009 1270 1291 1318 1730
Size: 293 93 382 105 261 21 27 412
Cuts at: 1730 2783 3215 3568 4230 136
Size: 1053 432 353 662 864
Fragments arranged by size:
1053 864 662 432 412 382 353 293
261 105 93 27 21
EcoNI CCTnn'n_nnAGG
Cuts at: 431 655 1249 431
Size: 224 594 4140
Fragments arranged by size:
4140 594 224
EcoO109I rG'GnC_Cy
Cuts at: 611 621 1338 1566 1629 1660 1705 2097 611
Size: 10 717 228 63 31 45 392 3472
Fragments arranged by size:
3472 717 392 228 63 45 31 10
EcoRI G'AATT_C
Cuts at: 1962 1962
Size: 4958
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1230 2977 1230
Size: 1747 3211
FseI GG_CCGG'CC
Cuts at: 1008 1008
Size: 4958
FspI TGC'GCA
Cuts at: 1489 2739 1489
Size: 1250 3708
FspAI rTGC'GCAy
Cuts at: 1489 1489
Size: 4958
HaeII r_GCGC'y
Cuts at: 407 1844 2392 2640 3870 4641 407
Size: 1437 548 248 1230 771 724
Fragments arranged by size:
1437 1230 771 724 548 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 392 424 1044 1076 2800 2830 2832 2862 392
Size: 32 620 32 1724 30 2 30 2488
Fragments arranged by size:
2488 1724 620 32 32 30 30 2
HincII GTy'rAC
Cuts at: 258 411 1997 258
Size: 153 1586 3219
Fragments arranged by size:
3219 1586 153
KasI G'GCGC_C
Cuts at: 403 2388 2636 403
Size: 1985 248 2725
Fragments arranged by size:
2725 1985 248
KpnI G_GTAC'C
Cuts at: 951 1978 951
Size: 1027 3931
MluI A'CGCG_T
Cuts at: 4391 4391
Size: 4958
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 535 3717 3901 535
Size: 3182 184 1592
Fragments arranged by size:
3182 1592 184
MscI TGG'CCA
Cuts at: 1822 2719 1822
Size: 897 4061
MslI CAynn'nnrTG
Cuts at: 1411 2947 3074 1411
Size: 1536 127 3295
Fragments arranged by size:
3295 1536 127
MspA1I CmG'CkG
Cuts at: 144 703 897 2069 2383 2743 3527 3772 144
Size: 559 194 1172 314 360 784 245 1330
Fragments arranged by size:
1330 1172 784 559 360 314 245 194
NaeI GCC'GGC
Cuts at: 1006 1558 3139 1006
Size: 552 1581 2825
Fragments arranged by size:
2825 1581 552
NarI GG'CG_CC
Cuts at: 404 2389 2637 404
Size: 1985 248 2725
Fragments arranged by size:
2725 1985 248
NcoI C'CATG_G
Cuts at: 107 953 107
Size: 846 4112
NdeI CA'TA_TG
Cuts at: 481 481
Size: 4958
NgoMIV G'CCGG_C
Cuts at: 1004 1556 3137 1004
Size: 552 1581 2825
Fragments arranged by size:
2825 1581 552
NspI r_CATG'y
Cuts at: 807 1089 2011 2511 4114 807
Size: 282 922 500 1603 1651
Fragments arranged by size:
1651 1603 922 500 282
PasI CC'CwG_GG
Cuts at: 624 624
Size: 4958
PciI A'CATG_T
Cuts at: 1085 4110 1085
Size: 3025 1933
PflMI CCAn_nnn'nTGG
Cuts at: 624 624
Size: 4958
PfoI T'CCnGG_A
Cuts at: 632 1166 1334 632
Size: 534 168 4256
Fragments arranged by size:
4256 534 168
PmeI GTTT'AAAC
Cuts at: 1957 1957
Size: 4958
PmlI CAC'GTG
Cuts at: 1422 1422
Size: 4958
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 817 849 1308 1340 3404 3436 817
Size: 32 459 32 2064 32 2339
Fragments arranged by size:
2339 2064 459 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1338 1660 1338
Size: 322 4636
PspOMI G'GGCC_C
Cuts at: 518 682 1566 518
Size: 164 884 3910
Fragments arranged by size:
3910 884 164
PspXI vC'TCGA_Gb
Cuts at: 1836 1836
Size: 4958
PstI C_TGCA'G
Cuts at: 896 902 1139 2005 2690 896
Size: 6 237 866 685 3164
Fragments arranged by size:
3164 866 685 237 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4958
PvuII CAG'CTG
Cuts at: 703 897 2383 2743 703
Size: 194 1486 360 2918
Fragments arranged by size:
2918 1486 360 194
RsrII CG'GwC_CG
Cuts at: 3153 3153
Size: 4958
SacI G_AGCT'C
Cuts at: 290 1972 290
Size: 1682 3276
SalI G'TCGA_C
Cuts at: 409 1995 409
Size: 1586 3372
SapI GCTCTTCn'nnn_
Cuts at: 2981 3191 2981
Size: 210 4748
SbfI CC_TGCA'GG
Cuts at: 1139 2005 1139
Size: 866 4092
ScaI AGT'ACT
Cuts at: 4475 4475
Size: 4958
SexAI A'CCwGG_T
Cuts at: 206 206
Size: 4958
SfcI C'TryA_G
Cuts at: 33 892 898 1135 2001 2686 3654 3845 33
Size: 859 6 237 866 685 968 191 1146
Fragments arranged by size:
1146 968 866 859 685 237 191 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 619 1513 619
Size: 894 4064
SfoI GGC'GCC
Cuts at: 405 2390 2638 405
Size: 1985 248 2725
Fragments arranged by size:
2725 1985 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4958
SmaI CCC'GGG
Cuts at: 1549 1980 1549
Size: 431 4527
SmlI C'TyrA_G
Cuts at: 428 1836 1930 2113 3028 3742 4004 428
Size: 1408 94 183 915 714 262 1382
Fragments arranged by size:
1408 1382 915 714 262 183 94
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4958
SphI G_CATG'C
Cuts at: 2011 2511 2011
Size: 500 4458
StuI AGG'CCT
Cuts at: 1141 1949 1141
Size: 808 4150
StyI C'CwwG_G
Cuts at: 107 221 953 1320 1452 2092 2446 107
Size: 114 732 367 132 640 354 2619
Fragments arranged by size:
2619 732 640 367 354 132 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1327 2541 2674 3133 3342 1327
Size: 1214 133 459 209 2943
Fragments arranged by size:
2943 1214 459 209 133
TatI w'GTAC_w
Cuts at: 4473 4473
Size: 4958
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 324 356 514 546 1330 1362 4475 4507 324
Size: 32 158 32 784 32 3113 32 775
Fragments arranged by size:
3113 784 775 158 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2755 2755
Size: 4958
XbaI T'CTAG_A
Cuts at: 978 1989 978
Size: 1011 3947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 159 453 2453 159
Size: 294 2000 2664
Fragments arranged by size:
2664 2000 294
XhoI C'TCGA_G
Cuts at: 428 1836 428
Size: 1408 3550
XmaI C'CCGG_G
Cuts at: 1547 1978 1547
Size: 431 4527
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AscI BaeI
BfrBI BmtI BsiWI BsmI BsmBI BspEI BsrGI BssHII
BstZ17I ClaI DraIII EcoRV HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PshAI PsiI PsrI SacII
SanDI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI