Restriction map for
pF1KB4091
(Circular) (Six-base) MAPSORT of: pF1KB4091.seq Check: 6148 from: 1 to: 4553
pF1KB4091 4553 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1370 1591 1370
Size: 221 4332
Acc65I G'GTAC_C
Cuts at: 1569 1569
Size: 4553
AclI AA'CG_TT
Cuts at: 4393 4393
Size: 4553
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 1113 2378 2810 3557 3825 311
Size: 802 1265 432 747 268 1039
Fragments arranged by size:
1265 1039 802 747 432 268
AfeI AGC'GCT
Cuts at: 115 115
Size: 4553
AflIII A'CryG_T
Cuts at: 1148 1905 3009 3986 1148
Size: 757 1104 977 1715
Fragments arranged by size:
1715 1104 977 757
AgeI A'CCGG_T
Cuts at: 2941 2941
Size: 4553
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 354 354
Size: 4553
AlwNI CAG_nnn'CTG
Cuts at: 1858 3425 1858
Size: 1567 2986
ApaLI G'TGCA_C
Cuts at: 3323 3323
Size: 4553
ApoI r'AATT_y
Cuts at: 550 762 1413 1557 3906 4162 550
Size: 212 651 144 2349 256 941
Fragments arranged by size:
2349 941 651 256 212 144
AseI AT'TA_AT
Cuts at: 20 20
Size: 4553
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
AvaI C'yCGr_G
Cuts at: 431 1573 431
Size: 1142 3411
BamHI G'GATC_C
Cuts at: 1578 1578
Size: 4553
BanI G'GyrC_C
Cuts at: 898 1569 1983 2231 2266 898
Size: 671 414 248 35 3185
Fragments arranged by size:
3185 671 414 248 35
BanII G_rGCy'C
Cuts at: 146 654 1567 2597 146
Size: 508 913 1030 2102
Fragments arranged by size:
2102 1030 913 508
BbeI G_GCGC'C
Cuts at: 1987 2235 1987
Size: 248 4305
BbsI GAAGACnn'nnnn_
Cuts at: 914 1020 914
Size: 106 4447
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1001 1035 1001
Size: 34 4519
BciVI GTATCCnnnnn_n'
Cuts at: 393 686 2451 3212 4553 393
Size: 293 1765 761 1341 393
Fragments arranged by size:
1765 1341 761 393 293
BclI T'GATC_A
Cuts at: 2073 2073
Size: 4553
BglII A'GATC_T
Cuts at: 513 2068 4105 513
Size: 1555 2037 961
Fragments arranged by size:
2037 1555 961
BlpI GC'TnA_GC
Cuts at: 145 1665 145
Size: 1520 3033
Bme1580I G_kGCm'C
Cuts at: 544 2178 2271 3327 544
Size: 1634 93 1056 1770
Fragments arranged by size:
1770 1634 1056 93
BmgBI CAC'GTC
Cuts at: 691 691
Size: 4553
BmrI ACTGGGnnnn_n'
Cuts at: 156 601 1765 1846 2181 4325 156
Size: 445 1164 81 335 2144 384
Fragments arranged by size:
2144 1164 445 384 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 4553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1104 1104
Size: 4553
Bpu10I CC'TnA_GC
Cuts at: 582 582
Size: 4553
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1729 2608 3100 3398 1729
Size: 879 492 298 2884
Fragments arranged by size:
2884 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4257 4257
Size: 4553
BsaAI yAC'GTr
Cuts at: 1906 1906
Size: 4553
BsaBI GATnn'nnATC
Cuts at: 6 191 2067 4104 6
Size: 185 1876 2037 455
Fragments arranged by size:
2037 1876 455 185
BsaHI Gr'CG_yC
Cuts at: 1984 2232 2934 4430 1984
Size: 248 702 1496 2107
Fragments arranged by size:
2107 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1742 2263 2941 3215 3362 3773 1742
Size: 521 678 274 147 411 2522
Fragments arranged by size:
2522 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1555 1555
Size: 4553
BseYI C'CCAG_C
Cuts at: 1527 1978 2736 3313 3809 1527
Size: 451 758 577 496 2271
Fragments arranged by size:
2271 758 577 496 451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 1518 324
Size: 1194 3359
BsiEI CG_ry'CG
Cuts at: 104 2095 2141 3349 104
Size: 1991 46 1208 1308
Fragments arranged by size:
1991 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 654 1567 2345 2535 3327 654
Size: 913 778 190 792 1880
Fragments arranged by size:
1880 913 792 778 190
BsmI GAATG_Cn'
Cuts at: 478 1206 478
Size: 728 3825
Bsp1286I G_dGCh'C
Cuts at: 146 544 654 1567 2178 2271 2345 2535 2597
Size: 398 110 913 611 93 74 190 62
Cuts at: 2597 3327 146
Size: 730 1372
Fragments arranged by size:
1372 913 730 611 398 190 110 93
74 62
BspEI T'CCGG_A
Cuts at: 3773 3773
Size: 4553
BspHI T'CATG_A
Cuts at: 3729 3729
Size: 4553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 322 1603 1817 2119 2500 322
Size: 1281 214 302 381 2375
Fragments arranged by size:
2375 1281 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2845 2899 4383 4548 50
Size: 2795 54 1484 165 55
Fragments arranged by size:
2795 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 887 1017 1353 1510 2465 887
Size: 130 336 157 955 2975
Fragments arranged by size:
2975 955 336 157 130
BsrFI r'CCGG_y
Cuts at: 2551 2732 2941 2551
Size: 181 209 4163
Fragments arranged by size:
4163 209 181
BsrGI T'GTAC_A
Cuts at: 508 884 1311 508
Size: 376 427 3750
Fragments arranged by size:
3750 427 376
BssSI C'ACGA_G
Cuts at: 2824 3182 2824
Size: 358 4195
BstAPI GCAn_nnn'nTGC
Cuts at: 1930 1930
Size: 4553
BstBI TT'CG_AA
Cuts at: 2914 2914
Size: 4553
BstYI r'GATC_y
Cuts at: 1 513 1248 1578 2045 2068 2403 2649 3661
Size: 512 735 330 467 23 335 246 1012
Cuts at: 3661 3747 3759 4105 1
Size: 86 12 346 449
Fragments arranged by size:
1012 735 512 467 449 346 335 330
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1371 1371
Size: 4553
BtgI C'CryG_G
Cuts at: 748 748
Size: 4553
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2503 2684 2847 2503
Size: 181 163 4209
Fragments arranged by size:
4209 181 163
BtsI GCAGTG_nn'
Cuts at: 1112 1796 1112
Size: 684 3869
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 480 515 480
Size: 35 4518
DraI TTT'AAA
Cuts at: 1552 1552
Size: 4553
DrdI GACnn_nn'nnGTC
Cuts at: 2259 3117 2259
Size: 858 3695
EaeI y'GGCC_r
Cuts at: 745 1215 2138 2312 2703 2730 4075 745
Size: 470 923 174 391 27 1345 1223
Fragments arranged by size:
1345 1223 923 470 391 174 27
EagI C'GGCC_G
Cuts at: 2138 2138
Size: 4553
EarI CTCTTCn'nnn_
Cuts at: 2576 2786 2576
Size: 210 4343
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2761 3071 3217 4363 2761
Size: 310 146 1146 2951
Fragments arranged by size:
2951 1146 310 146
EcoICRI GAG'CTC
Cuts at: 652 1565 652
Size: 913 3640
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 1104 1113 2378 2810 3557 3825 311
Size: 793 9 1265 432 747 268 1039
Fragments arranged by size:
1265 1039 793 747 432 268 9
EcoO109I rG'GnC_Cy
Cuts at: 334 1692 334
Size: 1358 3195
EcoRI G'AATT_C
Cuts at: 1557 1557
Size: 4553
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4553
FspI TGC'GCA
Cuts at: 2334 2334
Size: 4553
HaeII r_GCGC'y
Cuts at: 117 1987 2235 3257 4236 117
Size: 1870 248 1022 979 434
Fragments arranged by size:
1870 1022 979 434 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 438 470 2395 2425 2427 2457 289
Size: 32 117 32 1925 30 2 30 2385
Fragments arranged by size:
2385 1925 117 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1592 1592
Size: 4553
KasI G'GCGC_C
Cuts at: 1983 2231 1983
Size: 248 4305
KpnI G_GTAC'C
Cuts at: 1573 1573
Size: 4553
MluI A'CGCG_T
Cuts at: 3986 3986
Size: 4553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1285 3224 3408 1285
Size: 1939 184 2430
Fragments arranged by size:
2430 1939 184
MscI TGG'CCA
Cuts at: 1217 2314 1217
Size: 1097 3456
MslI CAynn'nnrTG
Cuts at: 1147 2542 2669 1147
Size: 1395 127 3031
Fragments arranged by size:
3031 1395 127
MspA1I CmG'CkG
Cuts at: 727 1664 1978 2338 3351 3596 727
Size: 937 314 360 1013 245 1684
Fragments arranged by size:
1684 1013 937 360 314 245
NaeI GCC'GGC
Cuts at: 2734 2734
Size: 4553
NarI GG'CG_CC
Cuts at: 1984 2232 1984
Size: 248 4305
NdeI CA'TA_TG
Cuts at: 948 948
Size: 4553
NgoMIV G'CCGG_C
Cuts at: 2732 2732
Size: 4553
NspI r_CATG'y
Cuts at: 1152 1606 2106 3013 1152
Size: 454 500 907 2692
Fragments arranged by size:
2692 907 500 454
PciI A'CATG_T
Cuts at: 1148 3009 1148
Size: 1861 2692
PmeI GTTT'AAAC
Cuts at: 1552 1552
Size: 4553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3724 3692
Size: 32 4521
PpuMI rG'GwC_Cy
Cuts at: 334 334
Size: 4553
PsiI TTA'TAA
Cuts at: 712 712
Size: 4553
PstI C_TGCA'G
Cuts at: 732 1600 2285 732
Size: 868 685 3000
Fragments arranged by size:
3000 868 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4553
PvuII CAG'CTG
Cuts at: 727 1978 2338 727
Size: 1251 360 2942
Fragments arranged by size:
2942 1251 360
RsrII CG'GwC_CG
Cuts at: 2748 2748
Size: 4553
SacI G_AGCT'C
Cuts at: 654 1567 654
Size: 913 3640
SalI G'TCGA_C
Cuts at: 1590 1590
Size: 4553
SapI GCTCTTCn'nnn_
Cuts at: 2576 2786 2576
Size: 210 4343
SbfI CC_TGCA'GG
Cuts at: 1600 1600
Size: 4553
ScaI AGT'ACT
Cuts at: 4070 4070
Size: 4553
SfcI C'TryA_G
Cuts at: 33 728 855 1596 2281 3274 3465 33
Size: 695 127 741 685 993 191 1121
Fragments arranged by size:
1121 993 741 695 685 191 127
SfoI GGC'GCC
Cuts at: 1985 2233 1985
Size: 248 4305
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
SmaI CCC'GGG
Cuts at: 1575 1575
Size: 4553
SmlI C'TyrA_G
Cuts at: 1708 2623 3115 3377 1708
Size: 915 492 262 2884
Fragments arranged by size:
2884 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4553
SphI G_CATG'C
Cuts at: 1606 2106 1606
Size: 500 4053
StyI C'CwwG_G
Cuts at: 801 1165 1486 1687 2041 801
Size: 364 321 201 354 3313
Fragments arranged by size:
3313 364 354 321 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2136 2269 2728 2937 2136
Size: 133 459 209 3752
Fragments arranged by size:
3752 459 209 133
TatI w'GTAC_w
Cuts at: 508 884 1311 4068 508
Size: 376 427 2757 993
Fragments arranged by size:
2757 993 427 376
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 333 365 4070 4102 333
Size: 32 3705 32 784
Fragments arranged by size:
3705 784 32 32
Tth111I GACn'n_nGTC
Cuts at: 2350 2350
Size: 4553
XbaI T'CTAG_A
Cuts at: 1584 1584
Size: 4553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 909 2048 909
Size: 1139 3414
XmaI C'CCGG_G
Cuts at: 1573 1573
Size: 4553
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BstZ17I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NspI PciI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI BsaXI BsiWI
BsmBI BssHII BstEII BstXI Bsu36I ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI