Restriction map for
pF1KB4086
(Circular) (Six-base) MAPSORT of: pF1KB4086.seq Check: 5371 from: 1 to: 4673
pF1KB4086 4673 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 247 812 1711 247
Size: 565 899 3209
Fragments arranged by size:
3209 899 565
Acc65I G'GTAC_C
Cuts at: 1689 1689
Size: 4673
AclI AA'CG_TT
Cuts at: 4513 4513
Size: 4673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1071 1133 1658 2498 2930 3677 3945 1071
Size: 62 525 840 432 747 268 1799
Fragments arranged by size:
1799 840 747 525 432 268 62
AflIII A'CryG_T
Cuts at: 1665 2025 3129 4106 1665
Size: 360 1104 977 2232
Fragments arranged by size:
2232 1104 977 360
AgeI A'CCGG_T
Cuts at: 3061 3061
Size: 4673
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 156 156
Size: 4673
AlwNI CAG_nnn'CTG
Cuts at: 511 1636 1978 3545 511
Size: 1125 342 1567 1639
Fragments arranged by size:
1639 1567 1125 342
ApaLI G'TGCA_C
Cuts at: 3443 3443
Size: 4673
ApoI r'AATT_y
Cuts at: 1677 4026 4282 1677
Size: 2349 256 2068
Fragments arranged by size:
2349 2068 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4673
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
AvaI C'yCGr_G
Cuts at: 1693 1693
Size: 4673
BamHI G'GATC_C
Cuts at: 1698 1698
Size: 4673
BanI G'GyrC_C
Cuts at: 1362 1689 2103 2351 2386 1362
Size: 327 414 248 35 3649
Fragments arranged by size:
3649 414 327 248 35
BanII G_rGCy'C
Cuts at: 1687 2717 1687
Size: 1030 3643
BbeI G_GCGC'C
Cuts at: 2107 2355 2107
Size: 248 4425
BbsI GAAGACnn'nnnn_
Cuts at: 427 507 1034 1365 427
Size: 80 527 331 3735
Fragments arranged by size:
3735 527 331 80
BbvCI CC'TCA_GC
Cuts at: 1193 1193
Size: 4673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4639
BciVI GTATCCnnnnn_n'
Cuts at: 2571 3332 4673 2571
Size: 761 1341 2571
Fragments arranged by size:
2571 1341 761
BclI T'GATC_A
Cuts at: 201 2193 201
Size: 1992 2681
BfrBI ATG'CAT
Cuts at: 490 490
Size: 4673
BglII A'GATC_T
Cuts at: 176 2188 4225 176
Size: 2012 2037 624
Fragments arranged by size:
2037 2012 624
BlpI GC'TnA_GC
Cuts at: 1785 1785
Size: 4673
Bme1580I G_kGCm'C
Cuts at: 2298 2391 3447 2298
Size: 93 1056 3524
Fragments arranged by size:
3524 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 828 1885 1966 2301 4445 828
Size: 1057 81 335 2144 1056
Fragments arranged by size:
2144 1057 1056 335 81
BmtI G_CTAG'C
Cuts at: 1076 1076
Size: 4673
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 561 3040 561
Size: 2479 2194
Bpu10I CC'TnA_GC
Cuts at: 1193 1193
Size: 4673
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 1849 2728 3220 3518 193
Size: 1656 879 492 298 1348
Fragments arranged by size:
1656 1348 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4377 4377
Size: 4673
BsaAI yAC'GTr
Cuts at: 2026 2026
Size: 4673
BsaBI GATnn'nnATC
Cuts at: 6 2187 4224 6
Size: 2181 2037 455
Fragments arranged by size:
2181 2037 455
BsaHI Gr'CG_yC
Cuts at: 1512 2104 2352 3054 4550 1512
Size: 592 248 702 1496 1635
Fragments arranged by size:
1635 1496 702 592 248
BsaWI w'CCGG_w
Cuts at: 1862 2383 3061 3335 3482 3893 1862
Size: 521 678 274 147 411 2642
Fragments arranged by size:
2642 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1598 1628 1598
Size: 30 4643
BseYI C'CCAG_C
Cuts at: 2098 2856 3433 3929 2098
Size: 758 577 496 2842
Fragments arranged by size:
2842 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 267
Size: 4673
BsiEI CG_ry'CG
Cuts at: 104 2215 2261 3469 104
Size: 2111 46 1208 1308
Fragments arranged by size:
2111 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1687 2465 2655 3447 1687
Size: 778 190 792 2913
Fragments arranged by size:
2913 792 778 190
BsmI GAATG_Cn'
Cuts at: 135 1560 135
Size: 1425 3248
Bsp1286I G_dGCh'C
Cuts at: 1687 2298 2391 2465 2655 2717 3447 1687
Size: 611 93 74 190 62 730 2913
Fragments arranged by size:
2913 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3893 3893
Size: 4673
BspHI T'CATG_A
Cuts at: 887 3849 887
Size: 2962 1711
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1723 1937 2239 2620 1723
Size: 214 302 381 3776
Fragments arranged by size:
3776 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2965 3019 4503 4668 50
Size: 2915 54 1484 165 55
Fragments arranged by size:
2915 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2585 2585
Size: 4673
BsrFI r'CCGG_y
Cuts at: 2671 2852 3061 2671
Size: 181 209 4283
Fragments arranged by size:
4283 209 181
BsrGI T'GTAC_A
Cuts at: 1139 1139
Size: 4673
BssSI C'ACGA_G
Cuts at: 2944 3302 2944
Size: 358 4315
BstAPI GCAn_nnn'nTGC
Cuts at: 2050 2050
Size: 4673
BstBI TT'CG_AA
Cuts at: 3034 3034
Size: 4673
BstXI CCAn_nnnn'nTGG
Cuts at: 258 258
Size: 4673
BstYI r'GATC_y
Cuts at: 1 176 824 1698 2165 2188 2523 2769 3781
Size: 175 648 874 467 23 335 246 1012
Cuts at: 3781 3867 3879 4225 1
Size: 86 12 346 449
Fragments arranged by size:
1012 874 648 467 449 346 335 246
175 86 23 12
BstZ17I GTA'TAC
Cuts at: 248 248
Size: 4673
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2623 2804 2967 2623
Size: 181 163 4329
Fragments arranged by size:
4329 181 163
BtsI GCAGTG_nn'
Cuts at: 1916 1916
Size: 4673
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 137 172 137
Size: 35 4638
DraI TTT'AAA
Cuts at: 1672 1672
Size: 4673
DrdI GACnn_nn'nnGTC
Cuts at: 2379 3237 2379
Size: 858 3815
EaeI y'GGCC_r
Cuts at: 145 2258 2432 2823 2850 4195 145
Size: 2113 174 391 27 1345 623
Fragments arranged by size:
2113 1345 623 391 174 27
EagI C'GGCC_G
Cuts at: 2258 2258
Size: 4673
EarI CTCTTCn'nnn_
Cuts at: 440 1090 1204 2696 2906 440
Size: 650 114 1492 210 2207
Fragments arranged by size:
2207 1492 650 210 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2881 3191 3337 4483 2881
Size: 310 146 1146 3071
Fragments arranged by size:
3071 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1685 1685
Size: 4673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1071 1133 1658 2498 2930 3677 3945 1071
Size: 62 525 840 432 747 268 1799
Fragments arranged by size:
1799 840 747 525 432 268 62
EcoO109I rG'GnC_Cy
Cuts at: 1812 1812
Size: 4673
EcoRI G'AATT_C
Cuts at: 1677 1677
Size: 4673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 294 2692 294
Size: 2398 2275
FspI TGC'GCA
Cuts at: 2454 2454
Size: 4673
HaeII r_GCGC'y
Cuts at: 2107 2355 3377 4356 2107
Size: 248 1022 979 2424
Fragments arranged by size:
2424 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 721 753 1318 1350 2515 2545 2547
Size: 32 400 32 565 32 1165 30 2
Cuts at: 2547 2577 289
Size: 30 2385
Fragments arranged by size:
2385 1165 565 400 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 528 1419 1712 528
Size: 891 293 3489
Fragments arranged by size:
3489 891 293
HindIII A'AGCT_T
Cuts at: 137 137
Size: 4673
HpaI GTT'AAC
Cuts at: 528 528
Size: 4673
KasI G'GCGC_C
Cuts at: 2103 2351 2103
Size: 248 4425
KpnI G_GTAC'C
Cuts at: 1693 1693
Size: 4673
MluI A'CGCG_T
Cuts at: 4106 4106
Size: 4673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 363 609 3344 3528 194
Size: 169 246 2735 184 1339
Fragments arranged by size:
2735 1339 246 184 169
MscI TGG'CCA
Cuts at: 147 2434 147
Size: 2287 2386
MslI CAynn'nnrTG
Cuts at: 319 710 2662 2789 319
Size: 391 1952 127 2203
Fragments arranged by size:
2203 1952 391 127
MspA1I CmG'CkG
Cuts at: 438 508 800 1645 1784 2098 2458 3471 3716
Size: 70 292 845 139 314 360 1013 245
Cuts at: 3716 438
Size: 1395
Fragments arranged by size:
1395 1013 845 360 314 292 245 139
70
NaeI GCC'GGC
Cuts at: 2854 2854
Size: 4673
NarI GG'CG_CC
Cuts at: 2104 2352 2104
Size: 248 4425
NdeI CA'TA_TG
Cuts at: 698 698
Size: 4673
NgoMIV G'CCGG_C
Cuts at: 2852 2852
Size: 4673
NheI G'CTAG_C
Cuts at: 1072 1072
Size: 4673
NsiI A_TGCA'T
Cuts at: 492 492
Size: 4673
NspI r_CATG'y
Cuts at: 494 709 1669 1726 2226 3133 494
Size: 215 960 57 500 907 2034
Fragments arranged by size:
2034 960 907 500 215 57
PasI CC'CwG_GG
Cuts at: 1431 1431
Size: 4673
PciI A'CATG_T
Cuts at: 1665 3129 1665
Size: 1464 3209
PflMI CCAn_nnn'nTGG
Cuts at: 548 1413 548
Size: 865 3808
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1491 1523 3812 3844 1491
Size: 32 2289 32 2320
Fragments arranged by size:
2320 2289 32 32
PstI C_TGCA'G
Cuts at: 1720 2405 1720
Size: 685 3988
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4673
PvuII CAG'CTG
Cuts at: 438 508 1645 2098 2458 438
Size: 70 1137 453 360 2653
Fragments arranged by size:
2653 1137 453 360 70
RsrII CG'GwC_CG
Cuts at: 2868 2868
Size: 4673
SacI G_AGCT'C
Cuts at: 1687 1687
Size: 4673
SalI G'TCGA_C
Cuts at: 1710 1710
Size: 4673
SapI GCTCTTCn'nnn_
Cuts at: 2696 2906 2696
Size: 210 4463
SbfI CC_TGCA'GG
Cuts at: 1720 1720
Size: 4673
ScaI AGT'ACT
Cuts at: 1186 4190 1186
Size: 3004 1669
SfcI C'TryA_G
Cuts at: 33 617 749 990 1716 2401 3394 3585 33
Size: 584 132 241 726 685 993 191 1121
Fragments arranged by size:
1121 993 726 685 584 241 191 132
SfoI GGC'GCC
Cuts at: 2105 2353 2105
Size: 248 4425
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
SmaI CCC'GGG
Cuts at: 1695 1695
Size: 4673
SmlI C'TyrA_G
Cuts at: 172 1828 2743 3235 3497 172
Size: 1656 915 492 262 1348
Fragments arranged by size:
1656 1348 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4673
SphI G_CATG'C
Cuts at: 494 1726 2226 494
Size: 1232 500 2941
Fragments arranged by size:
2941 1232 500
StyI C'CwwG_G
Cuts at: 1807 2161 1807
Size: 354 4319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1420 2256 2389 2848 3057 1420
Size: 836 133 459 209 3036
Fragments arranged by size:
3036 836 459 209 133
TatI w'GTAC_w
Cuts at: 1139 1184 1256 4188 1139
Size: 45 72 2932 1624
Fragments arranged by size:
2932 1624 72 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4190 4222 4190
Size: 32 4641
Tth111I GACn'n_nGTC
Cuts at: 2470 2470
Size: 4673
XbaI T'CTAG_A
Cuts at: 1704 1704
Size: 4673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2168 2168
Size: 4673
XmaI C'CCGG_G
Cuts at: 1693 1693
Size: 4673
XmnI GAAnn'nnTTC
Cuts at: 952 952
Size: 4673
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI
NsiI NspI PasI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BglI BmgBI BpmI BseRI BsiWI
BsmBI BssHII BstEII Bsu36I BtgI ClaI DraIII EcoNI
EcoRV FseI FspAI MfeI NcoI NotI NruI PacI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI