Restriction map for
pF1KB4085
(Circular) (Six-base) MAPSORT of: pF1KB4085.seq Check: 7418 from: 1 to: 6032
pF1KB4085 6032 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1848 1848
Size: 6032
AatII G_ACGT'C
Cuts at: 2185 2239 2185
Size: 54 5978
AccI GT'mk_AC
Cuts at: 308 3070 308
Size: 2762 3270
Acc65I G'GTAC_C
Cuts at: 621 1300 1944 3048 621
Size: 679 644 1104 3605
Fragments arranged by size:
3605 1104 679 644
AclI AA'CG_TT
Cuts at: 5872 5872
Size: 6032
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1268 2502 2515 3857 4289 4642 5304 1268
Size: 1234 13 1342 432 353 662 1996
Fragments arranged by size:
1996 1342 1234 662 432 353 13
AflIII A'CryG_T
Cuts at: 1724 2294 3384 5184 5465 1724
Size: 570 1090 1800 281 2291
Fragments arranged by size:
2291 1800 1090 570 281
AleI CACnn'nnGTG
Cuts at: 334 982 2419 334
Size: 648 1437 3947
Fragments arranged by size:
3947 1437 648
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1304 1304
Size: 6032
AlwNI CAG_nnn'CTG
Cuts at: 243 357 877 3337 4775 243
Size: 114 520 2460 1438 1500
Fragments arranged by size:
2460 1500 1438 520 114
ApaLI G'TGCA_C
Cuts at: 1732 2635 4870 1732
Size: 903 2235 2894
Fragments arranged by size:
2894 2235 903
ApoI r'AATT_y
Cuts at: 462 732 1031 1112 1154 2836 3036 5385 5641
Size: 270 299 81 42 1682 200 2349 256
Cuts at: 5641 462
Size: 853
Fragments arranged by size:
2349 1682 853 299 270 256 200 81
42
AseI AT'TA_AT
Cuts at: 20 20
Size: 6032
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6032
AvaI C'yCGr_G
Cuts at: 228 1576 2540 3052 228
Size: 1348 964 512 3208
Fragments arranged by size:
3208 1348 964 512
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1936 1969 1936
Size: 33 5999
BamHI G'GATC_C
Cuts at: 232 2265 3057 232
Size: 2033 792 3207
Fragments arranged by size:
3207 2033 792
BanI G'GyrC_C
Cuts at: 328 381 621 1300 1944 3048 3462 3710 3745
Size: 53 240 679 644 1104 414 248 35
Cuts at: 3745 328
Size: 2615
Fragments arranged by size:
2615 1104 679 644 414 248 240 53
35
BanII G_rGCy'C
Cuts at: 1498 2174 2494 3046 4076 1498
Size: 676 320 552 1030 3454
Fragments arranged by size:
3454 1030 676 552 320
BbeI G_GCGC'C
Cuts at: 3466 3714 3466
Size: 248 5784
BciVI GTATCCnnnnn_n'
Cuts at: 523 2712 3930 4986 6032 523
Size: 2189 1218 1056 1046 523
Fragments arranged by size:
2189 1218 1056 1046 523
BclI T'GATC_A
Cuts at: 2682 3552 2682
Size: 870 5162
BglII A'GATC_T
Cuts at: 3547 5584 3547
Size: 2037 3995
BlpI GC'TnA_GC
Cuts at: 2373 3144 2373
Size: 771 5261
Bme1580I G_kGCm'C
Cuts at: 1736 2639 3657 3750 4874 1736
Size: 903 1018 93 1124 2894
Fragments arranged by size:
2894 1124 1018 903 93
BmgBI CAC'GTC
Cuts at: 564 1230 1545 564
Size: 666 315 5051
Fragments arranged by size:
5051 666 315
BmrI ACTGGGnnnn_n'
Cuts at: 2558 3244 3325 3660 5804 2558
Size: 686 81 335 2144 2786
Fragments arranged by size:
2786 2144 686 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4399 4399
Size: 6032
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 448 541 1361 1807 2412 2729 385
Size: 63 93 820 446 605 317 3688
Fragments arranged by size:
3688 820 605 446 317 93 63
Bpu10I CC'TnA_GC
Cuts at: 350 1292 350
Size: 942 5090
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3208 4087 4801 5099 3208
Size: 879 714 298 4141
Fragments arranged by size:
4141 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2932 5736 2932
Size: 2804 3228
BsaAI yAC'GTr
Cuts at: 1725 1971 2073 3385 1725
Size: 246 102 1312 4372
Fragments arranged by size:
4372 1312 246 102
BsaBI GATnn'nnATC
Cuts at: 6 844 3546 5583 6
Size: 838 2702 2037 455
Fragments arranged by size:
2702 2037 838 455
BsaHI Gr'CG_yC
Cuts at: 403 1820 2182 2236 3463 3711 4413 5909 403
Size: 1417 362 54 1227 248 702 1496 526
Fragments arranged by size:
1496 1417 1227 702 526 362 248 54
BsaWI w'CCGG_w
Cuts at: 2812 3221 3742 4420 4831 4978 5252 2812
Size: 409 521 678 411 147 274 3592
Fragments arranged by size:
3592 678 521 411 409 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1256 1286 1916 1946 1987 2017 2590 2620 1256
Size: 30 630 30 41 30 573 30 4668
Fragments arranged by size:
4668 630 573 41 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 328 1795 2478 2659 2950 328
Size: 1467 683 181 291 3410
Fragments arranged by size:
3410 1467 683 291 181
BseYI C'CCAG_C
Cuts at: 236 937 3457 4215 4880 5288 236
Size: 701 2520 758 665 408 980
Fragments arranged by size:
2520 980 758 701 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 528
Size: 6032
BsiEI CG_ry'CG
Cuts at: 104 477 1954 2051 3574 3620 4850 104
Size: 373 1477 97 1523 46 1230 1286
Fragments arranged by size:
1523 1477 1286 1230 373 97 46
BsiHKAI G_wGCw'C
Cuts at: 1709 1736 2342 2428 2482 2639 3046 3824 4014
Size: 27 606 86 54 157 407 778 190
Cuts at: 4014 4874 1709
Size: 860 2867
Fragments arranged by size:
2867 860 778 606 407 190 157 86
54 27
BsmI GAATG_Cn'
Cuts at: 2654 2654
Size: 6032
Bsp1286I G_dGCh'C
Cuts at: 1498 1709 1736 2174 2342 2428 2482 2494 2639
Size: 211 27 438 168 86 54 12 145
Cuts at: 2639 3046 3657 3750 3824 4014 4076 4874 1498
Size: 407 611 93 74 190 62 798 2656
Fragments arranged by size:
2656 798 611 438 407 211 190 168
145 93 86 74 62 54 27 12
BspHI T'CATG_A
Cuts at: 4464 4464
Size: 6032
BspMI ACCTGCnnnn'nnnn_
Cuts at: 537 1186 1848 3082 3296 3598 3979 537
Size: 649 662 1234 214 302 381 2590
Fragments arranged by size:
2590 1234 662 649 381 302 214
BsrBI CCG'CTC
Cuts at: 50 287 1692 1745 4324 4378 5862 6027 50
Size: 237 1405 53 2579 54 1484 165 55
Fragments arranged by size:
2579 1484 1405 237 165 55 54 53
BsrDI GCAATG_nn'
Cuts at: 209 1307 3944 209
Size: 1098 2637 2297
Fragments arranged by size:
2637 2297 1098
BsrFI r'CCGG_y
Cuts at: 594 1822 1890 2040 2393 4030 4211 594
Size: 1228 68 150 353 1637 181 2415
Fragments arranged by size:
2415 1637 1228 353 181 150 68
BsrGI T'GTAC_A
Cuts at: 2081 2354 2081
Size: 273 5759
BssHII G'CGCG_C
Cuts at: 1811 1813 1986 2054 2399 1811
Size: 2 173 68 345 5444
Fragments arranged by size:
5444 345 173 68 2
BssSI C'ACGA_G
Cuts at: 1777 4303 5011 1777
Size: 2526 708 2798
Fragments arranged by size:
2798 2526 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3409 3409
Size: 6032
BstBI TT'CG_AA
Cuts at: 4393 4393
Size: 6032
BstXI CCAn_nnnn'nTGG
Cuts at: 2199 2334 2436 2199
Size: 135 102 5795
Fragments arranged by size:
5795 135 102
BstYI r'GATC_y
Cuts at: 1 232 2265 2851 3057 3524 3547 3882 4128
Size: 231 2033 586 206 467 23 335 246
Cuts at: 4128 4434 4446 4532 5584 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2033 1052 586 467 449 335 306 246
231 206 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 146 146
Size: 6032
BtgI C'CryG_G
Cuts at: 1190 1514 1529 1948 2163 2186 2192 2435 1190
Size: 324 15 419 215 23 6 243 4787
Fragments arranged by size:
4787 419 324 243 215 23 15 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 330 2292 3982 4163 4326 330
Size: 1962 1690 181 163 2036
Fragments arranged by size:
2036 1962 1690 181 163
BtsI GCAGTG_nn'
Cuts at: 1894 2357 2695 3275 1894
Size: 463 338 580 4651
Fragments arranged by size:
4651 580 463 338
ClaI AT'CG_AT
Cuts at: 848 848
Size: 6032
DraI TTT'AAA
Cuts at: 2555 2784 3031 2555
Size: 229 247 5556
Fragments arranged by size:
5556 247 229
DraIII CAC_nnn'GTG
Cuts at: 1974 1974
Size: 6032
DrdI GACnn_nn'nnGTC
Cuts at: 3738 5082 3738
Size: 1344 4688
EaeI y'GGCC_r
Cuts at: 288 1538 2189 2220 2692 3617 3791 4182 4209
Size: 1250 651 31 472 925 174 391 27
Cuts at: 4209 5554 288
Size: 1345 766
Fragments arranged by size:
1345 1250 925 766 651 472 391 174
31 27
EagI C'GGCC_G
Cuts at: 3617 3617
Size: 6032
EarI CTCTTCn'nnn_
Cuts at: 1130 4055 4265 1130
Size: 2925 210 2897
Fragments arranged by size:
2925 2897 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 388 510 2137 4240 4982 5128 5842 388
Size: 122 1627 2103 742 146 714 578
Fragments arranged by size:
2103 1627 742 714 578 146 122
EcoICRI GAG'CTC
Cuts at: 3044 3044
Size: 6032
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 448 541 1268 1361 1807 2412 2502 2515
Size: 63 93 727 93 446 605 90 13
Cuts at: 2515 2729 3857 4289 4642 5304 385
Size: 214 1128 432 353 662 1113
Fragments arranged by size:
1128 1113 727 662 605 446 432 353
214 93 93 90 63 13
EcoNI CCTnn'n_nnAGG
Cuts at: 427 2116 2644 427
Size: 1689 528 3815
Fragments arranged by size:
3815 1689 528
EcoO109I rG'GnC_Cy
Cuts at: 188 354 420 2226 2677 3171 188
Size: 166 66 1806 451 494 3049
Fragments arranged by size:
3049 1806 494 451 166 66
EcoRI G'AATT_C
Cuts at: 462 3036 462
Size: 2574 3458
EcoRV GAT'ATC
Cuts at: 687 687
Size: 6032
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4051 4051
Size: 6032
FspI TGC'GCA
Cuts at: 3813 3813
Size: 6032
HaeII r_GCGC'y
Cuts at: 674 1439 2261 2380 2435 3466 3714 4944 5715
Size: 765 822 119 55 1031 248 1230 771
Cuts at: 5715 674
Size: 991
Fragments arranged by size:
1230 1031 991 822 771 765 248 119
55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1321 1343 1353 1375 1441 1473 1480 1512 1987
Size: 22 10 22 66 32 7 32 475
Cuts at: 1987 2019 3874 3904 3906 3936 1321
Size: 32 1855 30 2 30 3417
Fragments arranged by size:
3417 1855 475 66 32 32 32 30
30 22 22 10 7 2
HincII GTy'rAC
Cuts at: 1164 2763 3071 1164
Size: 1599 308 4125
Fragments arranged by size:
4125 1599 308
HpaI GTT'AAC
Cuts at: 1164 1164
Size: 6032
KasI G'GCGC_C
Cuts at: 3462 3710 3462
Size: 248 5784
KpnI G_GTAC'C
Cuts at: 625 1304 1948 3052 625
Size: 679 644 1104 3605
Fragments arranged by size:
3605 1104 679 644
MluI A'CGCG_T
Cuts at: 5465 5465
Size: 6032
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1014 1307 4791 4975 1014
Size: 293 3484 184 2071
Fragments arranged by size:
3484 2071 293 184
MscI TGG'CCA
Cuts at: 2694 3793 2694
Size: 1099 4933
MslI CAynn'nnrTG
Cuts at: 334 982 2419 4021 4148 334
Size: 648 1437 1602 127 2218
Fragments arranged by size:
2218 1602 1437 648 127
MspA1I CmG'CkG
Cuts at: 543 1950 2535 2774 3143 3457 3817 4601 4846
Size: 1407 585 239 369 314 360 784 245
Cuts at: 4846 543
Size: 1729
Fragments arranged by size:
1729 1407 784 585 369 360 314 245
239
NaeI GCC'GGC
Cuts at: 1824 1892 4213 1824
Size: 68 2321 3643
Fragments arranged by size:
3643 2321 68
NarI GG'CG_CC
Cuts at: 3463 3711 3463
Size: 248 5784
NcoI C'CATG_G
Cuts at: 1190 1190
Size: 6032
NgoMIV G'CCGG_C
Cuts at: 1822 1890 4211 1822
Size: 68 2321 3643
Fragments arranged by size:
3643 2321 68
NspI r_CATG'y
Cuts at: 2625 3085 3585 5188 2625
Size: 460 500 1603 3469
Fragments arranged by size:
3469 1603 500 460
PciI A'CATG_T
Cuts at: 5184 5184
Size: 6032
PflMI CCAn_nnn'nTGG
Cuts at: 1974 1974
Size: 6032
PfoI T'CCnGG_A
Cuts at: 899 2111 2670 899
Size: 1212 559 4261
Fragments arranged by size:
4261 1212 559
PmeI GTTT'AAAC
Cuts at: 3031 3031
Size: 6032
PmlI CAC'GTG
Cuts at: 1971 2073 1971
Size: 102 5930
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4478 4510 4478
Size: 32 6000
PpuMI rG'GwC_Cy
Cuts at: 354 420 354
Size: 66 5966
PshAI GACnn'nnGTC
Cuts at: 917 917
Size: 6032
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 605 637 1573 1605 605
Size: 32 936 32 5032
Fragments arranged by size:
5032 936 32 32
PstI C_TGCA'G
Cuts at: 443 548 1766 1799 1859 2487 3079 3764 443
Size: 105 1218 33 60 628 592 685 2711
Fragments arranged by size:
2711 1218 685 628 592 105 60 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6032
PvuII CAG'CTG
Cuts at: 2535 2774 3457 3817 2535
Size: 239 683 360 4750
Fragments arranged by size:
4750 683 360 239
RsrII CG'GwC_CG
Cuts at: 4227 4227
Size: 6032
SacI G_AGCT'C
Cuts at: 3046 3046
Size: 6032
SacII CC_GC'GG
Cuts at: 1951 1951
Size: 6032
SalI G'TCGA_C
Cuts at: 3069 3069
Size: 6032
SapI GCTCTTCn'nnn_
Cuts at: 4055 4265 4055
Size: 210 5822
SbfI CC_TGCA'GG
Cuts at: 3079 3079
Size: 6032
ScaI AGT'ACT
Cuts at: 5549 5549
Size: 6032
SexAI A'CCwGG_T
Cuts at: 3023 3023
Size: 6032
SfcI C'TryA_G
Cuts at: 33 439 544 1762 1795 1855 2483 3075 3760
Size: 406 105 1218 33 60 628 592 685
Cuts at: 3760 4728 4919 33
Size: 968 191 1146
Fragments arranged by size:
1218 1146 968 685 628 592 406 191
105 60 33
SfoI GGC'GCC
Cuts at: 3464 3712 3464
Size: 248 5784
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6032
SgrAI Cr'CCGG_yG
Cuts at: 594 1822 594
Size: 1228 4804
SmaI CCC'GGG
Cuts at: 230 3054 230
Size: 2824 3208
SmlI C'TyrA_G
Cuts at: 2540 3187 4102 4816 5078 2540
Size: 647 915 714 262 3494
Fragments arranged by size:
3494 915 714 647 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6032
SphI G_CATG'C
Cuts at: 2625 3085 3585 2625
Size: 460 500 5072
Fragments arranged by size:
5072 500 460
StyI C'CwwG_G
Cuts at: 1190 3166 3520 1190
Size: 1976 354 3702
Fragments arranged by size:
3702 1976 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1948 3615 3748 4207 4416 1948
Size: 1667 133 459 209 3564
Fragments arranged by size:
3564 1667 459 209 133
TatI w'GTAC_w
Cuts at: 110 1580 2081 2354 5547 110
Size: 1470 501 273 3193 595
Fragments arranged by size:
3193 1470 595 501 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1256 1288 5549 5581 1256
Size: 32 4261 32 1707
Fragments arranged by size:
4261 1707 32 32
Tth111I GACn'n_nGTC
Cuts at: 1240 2238 3829 1240
Size: 998 1591 3443
Fragments arranged by size:
3443 1591 998
XbaI T'CTAG_A
Cuts at: 663 3063 663
Size: 2400 3632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3527 3527
Size: 6032
XhoI C'TCGA_G
Cuts at: 2540 2540
Size: 6032
XmaI C'CCGG_G
Cuts at: 228 3052 228
Size: 2824 3208
XmnI GAAnn'nnTTC
Cuts at: 785 785
Size: 6032
ZraI GAC'GTC
Cuts at: 2183 2237 2183
Size: 54 5978
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AloI ApaI AscI AvrII
BbsI BbvCI BcgI BfrBI BglI BmtI BsiWI BsmBI
BspEI BstEII BstZ17I CspCI FseI FspAI HindIII MfeI
NdeI NheI NotI NruI NsiI PacI PasI PsiI
PspOMI PspXI SanDI SfiI SnaBI SrfI SspI StuI
SwaI